[Analysis of the quality of preventive examinations of children (according to the survey of dentists)]. / Analiz kachestva provedeniya profilakticheskikh osmotrov detei (po dannym anketirovaniya vrachei-stomatologov).

OBJECTIVE: To improve the methodology for conducting preventive examinations of children by a dentist by identifying and preventing the risks of achieving their qualitative results. MATERIAL AND METHODS: A test version of the questionnaire was created, which was tested in a pilot study to assess validity and correction. A survey of 100 general dentists in Smolensk, Kaluga, Kaliningrad, Tula, who had previously participated in preventive dental examinations of children, was conducted. Questions were asked regarding the problems of organizing inspections, training, proposals for improving inspections. A comparative analysis of the risks of reducing the quality of examinations for each of the regions was carried out, proposals were made to improve the organization and conduct of medical examinations of children. RESULTS: As a result of the survey, a significant similarity of opinions of dentists in four cities of the Russian Federation on the problems and risks of annual preventive examinations of children was established. Among the weaknesses of the process, one should highlight the lack of time to examine the child; lack of specialized premises and a nurse; the absence of a unified form of a dental preventive examination card. This reduces the quality of diagnostics and continuity of medical care. Self-assessment by general practice dentists of their training in relation to diagnostics in children demonstrated a low level of knowledge of the issues of bite pathology, oral mucosa, and age periods of the dentoalveolar system. The lack of medical knowledge of more than 70% of doctors involved in preventive examinations of children is a key risk that requires immediate corrective action. CONCLUSION: The results of this study are the basis for the recommendation of advanced training of dentists at least once every three years on the subject of preventive examinations of children. The process of dental medical examination of the child population needs to be corrected at the legislative and executive levels.

Neuromuscular organization and haptoral armament of Polyclithrum ponticum (Monogenea: Gyrodactylidae).

Most gyrodactylids have a haptor armed with a pair of hamuli, two connecting bars and 16 marginal hooks. In some gyrodactylids, however, the haptor is disc-shaped and reinforced by additional sclerites. The genus Polyclithrum has arguably the most elaborate haptor in this group. This study aimed to gain better understanding of the anatomy of Polyclithrum by examining neuromusculature and haptoral armament of Polyclithrum ponticum, a species parasitizing Mugil cephalus in the Black Sea, with emphasis on haptoral sclerites and musculature in connection with host-attachment mechanisms. Musculature was stained by phalloidin, the nervous system by anti-serotonin and anti-FMRFamide antibodies, and haptoral sclerites were visualized in reflected light. The study provided new information on sclerites: in addition to previously described supplementary sclerites (A1-6), ear-shaped sclerites (ESSs) and two paired groups of ribs, reflected light revealed a rod-shaped process on the ESSs and a pair of small posterior sclerites. The sclerites were shown to be operated by 16 muscles, the most prominent of which were two transverse muscles connecting the hamular roots, three muscles attached to sclerite A2, the muscle fibres of anterior ribs and a set of extrinsic muscles. The nervous system consists of a pair of cerebral ganglia connected by a commissure and three pairs of nerve cords that unite in the haptor to form a loop between the opposite cords. The arrangement of sclerites and muscles suggests that Polyclithrum initiates the attachment by clamping a host's surface with longitudinally folded haptor and then secures its position with marginal hooks.

[INDUCTION OF EFFECTORS OF INNATE AND ADAPTIVE IMMUNITY IN THE PROCESS OF THERAPY OF TOPIC FORM OF RECOMBINANT INTERFERON- α2b DURING RESPIRATORY INFECTIONS IN PREGNANT].

AIM: Study immunologic phenotype of lymphocytes in the process of therapy of topic form of recombinant interferon-α2b during respiratory infections in pregnant. MATERIALS AND METHODS: 74 pregnant women from 14 weeks of gestation took part in the study, among them 55 - within 24 hours with symptoms of acute respiratory infection (ARI) of light and me- dium, severe course of infection, who do not need hospitalization. Group I - 34 pregnant womenwithARI receivingbasic therapywith human recombinant interferon-(α2b in gelform. Group II - 21 pregnant with ARI receiving only basic therapy. Control group had 19 pregnant women without signs of ARI. Relative content of principle lymphocyte subpopulations was studied by flow cytofluorimetry: CD3+, CD3+CD4+, CD3+CD8+, CD3-CD19+, CD3- CD16+56+, CD3-CD8+; immune regulatory indexwas calculated inblood within 24 hours from the onset of the disease and 8 - 10 days later. RESULTS: A disbalance of lymphocyte sub- populations was noted in pregnant women with light or medium severity course of acute respiratory infections, that was characterized by an increased content of CD3-CD16+56+ and CD3+CD8+, as well as a reduced content of CD3+ and CD3+CD8+. Inclusion of a topical form of recombinant interferon-α2b during the first days of development ofthe disease has a systemic effect on cell immunity and results in normalization of subpopulation compo- sition of blood lymphocytes that is characteristic for physiological course of pregnancy. CONCLUSION: Administration of topic form of recombinant interferon in pregnant with light or medium severity of ARI can be accompanied by activation of factors of innate and adaptive immunity.

[Influence of the Concentration of Dissolved Oxygen on Embryonic Development of the Common Toad (Bufo bufo)].

Several series of experiments investigating the influence of dissolved oxygen concentrations on the growth rates and mortality in the embryogenesis of the common toad Bufo bufo were carried out. The experiments showed that, when the eggs develop singly, the lack of oxygen does not lead to an increase in mortality by the time of hatching and results only in a change in the dynamics of mortality: mortality occurs at an earlier stage of development than in the conditions of normal access to oxygen. Taking into account the combined effect of the density of eggs and the dissolved oxygen concentration, we increase the accuracy of analysis of the experimental results and improve the interpretation of the results. In the conditions of different initial density of eggs, the impact of the concentration of dissolved oxygen on mortality and rates of development of the common toad embryos is manifested in different ways. At high density, only a small percentage of embryos survives by the time of hatching, and the embryos are significantly behind in their development compared with the individuals that developed in normal oxygen conditions. The lack of oxygen dissolved in the water slows down the development of embryos of the common toad.

[On psychologic profile of locomotive crew workers in Russian Railways JSC and its role in accidents prevention].

The article covers personality characters of locomotive crew workers of Russian Railways JSC, and specifying a block of methods for psychologic support of these workers' occupational activities. According to the results obtained, the authors presented a thorough description of characterologic, motivational personality traits characterizing a select of locomotive crew workers, and analyzed a relationship between the traits and levels of accidents in the workers' occupational activity.

[Safety criteria for blood refusion in military field surgery and trauma surgery].

Massive intracavitary hemorrhage in case if severe concomitant injury is the most frequent cause of death. Necessity of blood loss replacement requires effective and safe methods. One of them is blood reinfusion - technically less complicated method. The article shows advantages of using the hardware and filtration methods of blood sampling and reinfusion, developed scale rapid diagnosis of the level of hemolysis of autologous blood. Using of the method of filtration blood sampling provides 2X minimal amount of free hemoglobin than using an aspirator and reinfusion of blood with the contents therein to free hemoglobin 2.0g/ L prevents the risk of developing the syndrome and massive blood transfusion reduces the incidence of deaths at 11%. In patients with massive intracavitary hemorrhage it is advisable to use the method of semi-quantitative rapid analysis of free hemoglobin color scale.

[Structure and dynamics of depressive disorders in patients with a new coronavirus infection]. / Struktura i dinamika depressivnykh rasstroistv u patsientok, perenesshikh novuyu koronavirusnuyu infektsiyu (COVID-19).

OBJECTIVE: To analyze the dynamics of depressive disorders in patients who underwent COVID-19. MATERIAL AND METHODS: The study included female patients (n=30) with mean age 35.4±3.0 years, who received inpatient treatment of depressive spectrum symptoms in accordance with ICD-10 rubrics F31-F34 and who have undergone COVID-19 (U04.9, U07.1, U07.2). Clinical-psychopathological and statistical methods were used. RESULTS: Among all patients, who underwent inpatient treatment for affective disorders, the most common symptoms were: low mood (96.7%), anxiety (83.3%), decreased interest in daily activities (40%), loss of appetite (36.7%). In patients who have had depressive symptoms before COVID-19, an increase in the number of affective spectrum symptoms (suicidal thoughts - 83.3%), as well as in asthenic manifestations in the form of increased fatigue (60%), sleep disorders (73.3%), feelings of fatigue, weakness (60%), emotional lability (40%). CONCLUSION: In patients with depressive disorders who underwent COVID-19, hypothymia is most often accompanied by anxiety, suicidal thoughts, as well as asthenic manifestations in the form of fatigue, sleep disturbances, and emotional lability. An increase in the number of asthenic spectrum symptoms in patients with depressive disorders may indicate a possible link between COVID-19 and affective pathology. The impact of coronavirus infection on the structure and dynamics of depressive disorders requires further study, taking into account the severity of affective symptoms, the impact of psychopharmacotherapy, and the severity of the infectious disease.

Molecular-genetic approaches to species identif ication of platyhelminthes of the genus Ligophorus (Monogenea) parasitising f lathead mullet.

Mugil cephalus L., 1758 (flathead mullet) is a valuable commercial fish and a promising object of artificial breeding in the Black Sea and the Sea of Azov, and the study of its parasite fauna is important for fishery and mariculture. Monogeneans of the genus Ligophorus are common ectoparasites dwelling on the gills of mullets. Two representatives of this genus parasitise flathead mullet in the Azov-Black Sea region, namely Ligophorus mediterraneus Sarabeev, Balbuena et Euzet, 2005 and Ligophorus cephali Rubtsova, Balbuena, Sarabeev, Blasco- Costa et Euzet, 2006. Morphological identification of these species requires spending much time and a high level of experience in monogenean taxonomy. For quick and correct species identification of these parasites, we have developed a genotyping approach based on the polymerase chain reaction of allele-specific gene sites for various Monogenea species. A fragment of the 28S ribosomal gene, which includes conserved and variable sites, was chosen as a genetic marker. Three approaches were used as follows: amplified fragment length analysis, allelespecific PCR with endpoint detection and allele-specific real-time PCR using SYBR Green intercalating dye. The first approach was by obtaining PCR products of different lengths that were specific either to L. mediterraneus or to L. cephali. This approach was implemented due to the presence of several variable sites located at a distance from each other. The PCR mixture contained three primers: one forward and two reverse. The forward primer was complementary to the conserved site, which did not differ between species. Reverse primers were speciesspecific and, for each species, they were complementary to different DNA regions located 100 bp apart. As a result, L. mediterraneus was characterized by shorter amplicons than L. cephali. For the second and third approaches, a pair of primers was designed according to the following principle: the forward primer was complementary to both species, since it was selected for the conserved gene region. Reverse primers were species-specific and were designed for the 28S variable region. The two parasite species were distinguished by three-point mutations. Thus, one pair of primers was complementary to L. mediterraneus, the other, to L. cephali. The amplified fragment length analysis and the allele-specific real-time PCR demonstrated 100 % coincidence of genotyping results compared with Sanger sequencing. The developed genotyping protocols can be used not only to distinguish two species of Ligophorus from flathead mullet in ecological studies and veterinary practice but also for further development of similar approaches for other monogeneans, among which there are many pathogenic species.

Solid-phase analytical derivatization as a tool for the quantification of steroid hormones in human urine with HPLC-Q-ToF detection.

A possibility of application of solid-phase analytical derivatization (SPAD) for the quantification of seven steroid hormones (testosterone, dihydrotestosterone, cortisone, cortisol, progesterone, 11α-hydroxyprogesterone, and estrone) in human urine was evaluated. To prepare urine samples for instrumental analysis, SPAD with hydroxylamine was applied after enzymatic hydrolysis of the sample. To achieve high recovery values, extraction and derivatization conditions were optimized. Cartridges packed with end-capped octadecylsilyl silica sorbent provided optimum extraction of target analytes, while the reaction with hydroxylamine in the cartridge was found as a simple and efficient way for the chemical derivatization of steroids. The obtained derivatives were detected by using reversed-phase ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The proposed procedure was validated and applied to the analysis of real urine samples to prove the applicability of the proposed method for the routine analysis.

[Predictors of suicidal behaviour in adolescents with depressive disorders]. / Prediktory suitsidal'nogo povedeniia u podrostkov s depressivnymi rasstroistvami.

A review of recent publications is devoted to risk factors for suicidal behavior in adolescents with depressive disorders. The authors summarized Russian and international studies on the clinical course of affective disorders, in particular bipolar disorders, their clinical heterogeneity and incompleteness in adolescence. Particular attention is given to the complexity of early identification of predictors of suicidal behavior in depressive disorders due to comprehensiveness of exogenous and endogenous effects characteristic of adolescence. The role of genetic loading for affective disorders, problems of children having parents with affective disorders, social and psychological factors (violence in families, school bullying etc.) and risks of suicidal behavior are described. It is of great significance to investigate premorbid adjustment, subaffective manifestations of disorders in adolescence to predict the trajectory of the disease, its complications in the form of suicidal behavior and opportunities for early intervention.

[Ultrastructural changes in the MP3 neuron of the mollusk Lymnaea stagnalis after cryopreservation of the isolated brain].

Investigation of a possibility of long-term storage of frozen (-196 degrees C) viable neurons and nervous tissue is one of the central present day problems. In this study ultrastructural changes in neurons of frozen-thawed snail brain were examined as a function of time. We studied the influence of cryopreservation, cryoprotectant (Me2SO), cooling to 4-6 degrees C, and a prolonged incubation in physiological solution at 4-6 degrees C on dictyosomes of Golgi apparatus, endoplasmic reticulum (ER) cisternae and mitochondria. It has been found that responses of these intracellular structures of cryopreserved neurons to the above influences are similar: dissociation of Golgi dictyosomes, swelling of endoplasmic reticulum cisternae and mitochondrial cristae. Both freezing-thawing and cryoprotectant were seen to cause an increase in the number of lysosomes, liposomes, myelin-like structures, and to form large vacuoles. The structural changes in molluscan neurons caused by cryopreservation with Me2SO (2 M) were reversible.

[A description of a new species Gyrodactylus moldovicus sp. n. (Monogenea: Gyrodactylidae) from the European mudminnow Umbra krameri Walbaum, 1792 from the lower Dnester basin].

Gyrodactylus moldovicus sp. n. found on gills, body and in nasal cavities of the European mudminnow (Umbra krameri) differs from G. slovacicus Ergens, 1963 also living on the this host by bigger size of the body, anchors and marginal hooks; from G. cylindriformes Mueller et Van Cleave, 1932 living on the American mudminnow Umbra limi--by bigger size of the body; from G. limi Wood et Mizelle, 1957 also from U. limi--by the form of ventral and dorsal bars and form of marginal hooks. It differs from other freshwater gyrodactylids by special type of marginal hooks which have a hook-like end of the blade. Gyrodactylus moldovicus, G. slovacicus and G. limi have marginal hooks of quite different morphological types. By the morphology of anchors, ventral and sometimes dorsal bars and also morphology of cirrus, G. moldovicus is most related to three species from Cyprininae: G. stankovichi Ergens, 1970, G. longoacuminatus Zitnan, 1964 f. typica and G. shulmani Ling, 1962.

[Ultrastructure of the cells and DNA synthesis in skeletal muscle regeneration. A study of the regeneration of the frog sartorius muscle by an electron microscopic autoradiographic method]. / Ul'trastruktura kletok i sintez DNK pri regeneratsii skeletnykh myshts. Issledovanie regeneratsii portniazhkoi myshtsy liagushki metodom.

The ultrastructure of cells of the regenerating frog's sartorius muscle and their capacity to synthesize DNA was studied by means of 3H-thymidine (3HT) electron microscope autoradiography. On the 8-17th post injury (p.i.) days, 2 hours following 3HT administration, only mononuclear cells were seen labeled, the myotube nuclei incorporating no 3HT. Along with the endothelial cells, fibroblasts, phagocytes and cells identified conventionally as myoblasts, satellite cells examined from both necrotic and viable parts of injured myofibers were labeled. No myoblast sequestration from the injured myofibers occurred. By the 13-15th p.i. days, numerous myoblast-like cells are accumulated beneath the glycocalix layer covering the free ends of myotubes which are rich in ribosomes and display an active sarcomerogenesis. Some of these myoblast-like cells become labeled after 3HT pulse. The 13 day p.i. regenerates examined 72 hours following 3HT injection display labeling in numerous myotube nuclei. This is indicative of the myoblast fusion, which is believed to play a principal role in the regenerative somatic myogenesis. Within the myonuclei adjacent to the areas of the regeneration, membranous and/or fibrillar structures of an unknown origin were frequently observed.

[Functional activity of ganglion neurons from the pond snail upon temperature variations]. / Issledovanie funktsional'noi aktivnosti neironov gangliev bol'shogo prudovika pri izmenenii temperatury.

Changes in the functional state of the synthetic apparatus of neurons of the peripharyngeal ring of the mollusk Lymnaea stagnalis upon elevating the temperature of the habitat from 4 to 18-22 degrees C were studied. In the first series of experiments, it was found that the synthetic activity of neurons of the great and small parietal, visceral, and pleural ganglia increases on the average by 70%. In the second series of experiments, changes in the average synthetic activity for all ganglia at 4.0, 6.5, 13.0, 16.8, 20.5, 21.5, 21.9, and 22.0 degrees C were studied. The increase in the synthetic activity of neuron cytoplasm (35% higher than the control values at 4.0 degrees C) was maximal at 13.0 degrees C; then the synthetic activity decreased to reach the level 20% higher than the control. The physiological range of temperature-dependent changes in the functional state of neurons was determined.

[Cryopreserved neurons of a mollusc are capable of morphological differentiation in culture in vitro]. / Kriokonservirovannye neirony molluska sposobny k morfoligicheskoi diffentsirovke v kul'ture in vitro.

It was shown in culture in vitro that neurons isolated from the cryopreserved brain of adult molluscs Lymnaea stagnalis L. retain viability. Isolated brains were frozen in liquid nitrogen vapors at a rate of 400-500 degrees C/min in the presence of 2 M dimethylsulfoxide. The samples were then plunged into liquid nitrogen and stored from 1 month to 2 years. Upon thawing and removing the cryoprotectant, the neurons were isolated from the brain and then introduced into a cellular culture in vitro. It was shown that the thawed neurons were capable of regenerating new nerve processes similar to those formed by unfrozen neurons in the control.

[The functional activity of anti-endotoxin factors in viral hepatitis A and B]. / Funktsional'naia aktivnost' antiéndotoksinovykh faktorov pri virusnykh gepatitakh A i B.

Bacterial endotoxins (BE) that are lipopolysaccharide complexes (LPS) are a structural component of the external membrane of gram-negative bacteria. In normalcy, BE interact with many types of cells in the mammals. In terms of the concentration, BE may cause cell damage or stimulate the production of many biological mediators, such as interleukins, prostaglandinds, alpha-TNF. Many gastrointestinal bacteria in humans are gram-negative and BE constantly enter the blood. In health, the absence of a toxic response to BE is explained by the presence of natural humoral and cellular antiendotoxic systems and the hepatic absorption of LPS. In patients with hepatitis A and B, the following indices of the blood antiendotoxic systems were determined: the level of antiendotoxic antibodies to Re-chemotype glycolipids was assessed by the passive hemagglutination reaction in the "Antiendotox-1-test"; the count of polymorphonuclear leukocyte (PMNL) fixating LPS on their own surface and endotoxin binding function of PMNL was in vitro measured by the strain ELISA and sandwich ELISA with Re-glycolipids, respectively (LPS-test); the endotoxin fixation function of serum high density lipoproteins (HDL) was also assessed. The humoral and cellular antiendotoxic systems in patients with mild advanced hepatitis A and B was studied when the disease was most clinically significant, at an early convalescence, and at convalescence itself. Finally, the findings indicate that there is a significant decrease in Re-antibody levels and there is a greater absorption ability of HDL than that in the control. Six different types of an antiendotoxic fixation reaction of PMNL were identified in patients with viral hepatitis in the different periods of the disease. The alterations observed may play an important role in the pathogenesis of toxemia in patients with viral hepatitis.

[Nucleolus-forming regions in the chromosomes in continuous cell cultures]. / Iadryshkoobrazuiushchie raiony v khromosomakh perevivaemykh kul'tur kletok.

Localization of nucleoli-forming (NF) areas in chromosomes of continuous cell cultures of African green monkey kidney Vero, CV-1, GMK, BGM, BS-C-1. Macaca rhesus kidney LLC-MK2, dog kidney MDCK, pig kidney SPEV, PK-15 was studied. The NF-areas in chromosomes of African green monkey, M. rhesus, and pig cell cultures were shown to be localized in sites of secondary chromosomal stangulation, and there was also a definite correlation between the intensity of staining of NF-areas and sizes of the zone of secondary strangulation. In chromosomes of MDCK cell line, NF-areas are located in telomere ends of six-nine chromosomes, and association of two chromosomes by NF-areas was observed. The possibility of using the method of identification of nucleoi-forming areas for the evaluation of productivity of continuous cell cultures is discussed.

[The role of Fas/FasL system in induction of hepatocyte apoptosis in chronic viral hepatitides]. / Rol' sistemy Fas/FasL v induktsii apoptoza gepatotsitov pri khronicheskikh virusnykh gepatitakh.

The aim of the study was assessment of hepatocyte apoptosis depending on expression of Fas and FasL proteins by various liver cells in patients with chronic viral hepatitis B (CVHB) or chronic viral hepatitis C (CVHC). The symptoms of hepatocyte apoptosis were observed in 3 of 12 patients with CVHB and in 9 of 14 patients with CVHC, the proportion of apoptotic cells being 12-65%. Hepatocytes of healthy people and patients with hepatitis B or C express Fas protein in the cytoplasm diffusely, as granules or on cell membrane. In health, hepatocytes do not express FasL, but in CVH they do. The highest apoptosis was observed in Fas protein location as granules in cytoplasm or in their preferable location on the cell membrane. The severity of hepatocyte apoptosis in CVH directly correlated with FasL expression by the cells of the lymphoid-histiocytic infiltrate in the liver and inversely correlated with FasL expression by hepatocytes. Thus, a great part of hepatocytes in CVH are killed by the virus; Fas/FasL interaction is leading in damage to hepatocytes in CVH.

[A redescription of Gyrodactylus atherinae Bychowsky, 1933 based on the collection of B. E. Bychowsky of 1947 from Atherina boyeri pontica in the Black Sea]. / Pereopisanie Gyrodactylus atherinae Bychowsky, 1933 po sboram B E. Bykhovskogo 1947 goda s Atherina boyeri pontica iz Chernogo moria.

A redescription and comparative diagnosis of Gyrodactylus atherinae Bychowsky, 1933 is made based on the material collected of B. E. Bychowsky from Atherina boyeri pontica caught near Karadag (Black Sea) in summer of 1947.

[Policlithrum ponticum sp. n. (Monogenea: Gyrodactylidae: Polyclithrinae) from Mugil cephalus from the Black Sea and problems of suprageneric systematics of the gydrodactylids]. / Polyclithrum ponticum sp. n. (Monogenea: Gyrodactylidae: Polyclithrinae) s Mugil cephalus iz Chernogo Moria i problema nadrodovoi sistematiki gidrodaktilid.

Polyclithrum ponticum sp. n. is described and P. mugilini Rogers, 1967 is redescribed. Both monogenean species are parasites of Mugil cephalus in the Black Sea. The new species differs from P. mugilini, P. alberti and P. boegeri by the lesser size of anchors, while it is distinguished from P. corallense by the larger size of these structures. P. ponticum sp. n. differs from all formerly described species by the greater length of dorsal connective bar. In both species from the Black Sea, "ear-like" structures situated near the external roots of anchors are described for the first time. It is suggested, that these structures take part in longitudinal, two-lobe folding of the haptor. The process of opening the haptor is probably performed by the additional bars of the haptor (bars 2 and 3 after: Rogers, 1967), joined to each other and with the anchors. The fifth pair of additional bars (Ernst e. a., 2000) derives from the "beard" of ventral connective bar and is united with its basal part. The sixth pair of additional bars (Ernst e. a., 2000) is considered as a typical "ribs" of the haptor, and therefore the "ribs" are represented by three pairs. Differences between marginal hooks of P. ponticum sp. n. and P. mugilini are insignificant, that probably depends on the presence of "ribs" of the haptor. Based on the subdivision of marginal hooks into two groups, the presence of additional supporting structure in the haptor, and the presence of the seminal receptacle, it is suggested that the subfamily Polyclithrinae Rogers, 1967 should include the genera Polyclithrum Rogers, 1967, Swingleus Rogers, 1969, Macrogyrodactylus Mamlberg, 1959, and probably Fundulotrema Hargis, 1955. Based on such characters as the lack of the anchors, the presence of suckers in the haptor, and ovipositing of eggs, it seems to be expedient to use the following taxa in systematics of gyrodactylids: Isancistrinae Fuhrmann, 1928 (genera Isancistrum, Anacanthocotyle); Gyrdicotylinae Vercammen-Grandjean, 1960 (Gyrdicotyle) and Ooegyrodactylinae Harris, 1983 (genera Phanerothecium, Ooegyrodactylus, Nothogyrodactylus, Hyperopletes).