ABSTRACT
Investigating the effect of different phases on the optical performance is crucial for thermal sensing phosphor materials. Ba(2-x)SrxMgWO6:Er3+, Yb3+, K+ double perovskite phosphors were successfully prepared using a high-temperature solid-phase method. The dominant up-conversion luminescent (UCL) mechanism was deduced by analyzing the power-dependence spectra and energy level diagrams. By X-ray diffraction tests and tolerance factor calculations, it was demonstrated that the substitution of Sr2+ ions for Ba2+ ions led to the phase changing from cubic to tetragonal. The phase transition led to a decrease in the crystallographic symmetry of the compounds and changes in the optical thermometric properties. The optical temperature sensing properties were investigated using the fluorescence intensity ratio of thermally coupled energy levels (2H11/2 and 4S3/2 to the ground state energy level 4I15/2) of Er3+ ions in Ba2MgWO6, BaSrMgWO6 and Sr2MgWO6. The maximum absolute sensitivities obtained for Ba2MgWO6, BaSrMgWO6 and Sr2MgWO6 doped with 7% Er3+, 2% Yb3+ and 9% K+ were 6.77 × 10-4 K-1, 10.09 × 10-4 K-1 and 23.4 × 10-4 K-1, respectively. The comparison revealed that the phase transition caused an increase in the luminescence intensity and absolute sensitivity. This provides a useful pathway for modulating the subsequent thermometric performance.
ABSTRACT
The characteristics of neonatal immune cells display intrinsic differences compared with adult immune cells. Therefore, a comprehensive analysis of key gene expression regulation is required to understand the response of the human fetal immune system to infections. Here, we applied single-cell RNA sequencing (scRNA-seq) and single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) to systematically profile umbilical cord blood (UCB) nucleated cells and peripheral blood mononuclear cells (PBMCs) to identify their composition and differentially expressed genes. The immune cells in neonatal UCB demonstrated the expression of key genes, such as HBG2, NFKBIA, JUN, FOS, and TNFAIP3. In contrast, natural killer and T cells, which are constituents of adult PBMCs, exhibited high cytotoxic gene expression. Furthermore, we obtained similar results from the data of scATAC-seq by identifying the status of chromatin accessibility of key genes. Therefore, scRNA-seq and scATAC-seq of neonatal UCB nucleated cells and adult PBMCs could serve as an invaluable resource for elucidating the regulatory mechanisms of responses of distinct immune cell types and further identifying the differences between neonatal and adult immune responses to predict the potential underlying mechanism for neonatal immune tolerance.
Subject(s)
Fetal Blood , Single-Cell Analysis , Adult , Chromatin/metabolism , Humans , Immune Tolerance/genetics , Infant, Newborn , Leukocytes, Mononuclear/metabolism , Single-Cell Analysis/methods , Transposases/geneticsABSTRACT
Optical anti-counterfeiting has been developed as a promising optical-sensing technique. A self-activated KGaSiO4 phosphor was successfully prepared using the traditional solid-state method. The photoluminescence spectra of the as-synthesized phosphors indicate that the ultra-narrow band emission with green light peak at 503 nm is obtained when phosphors are excited by 254 nm UV light. Additionally, the measured afterglow curve shows that the emission of this phosphor can last more than 1200 s after UV excitation stops, which indicates that KGaSiO4 is a potential candidate for anti-counterfeiting materials. The luminescent and decay mechanism are discussed by theoretical calculation and thermo-luminescent spectra in detail. The theoretical model can provide support for explaining the mechanism of narrow band or persistent phosphor.
ABSTRACT
A series of Ca15(PO4)2(SiO4)6:xCe3+,yTb3+ phosphors have been prepared by a high-temperature solid-state reaction. Under the excitation of near-UV with 371 nm wavelength, Ca15(PO4)2(SiO4)6:xCe3+ phosphors exhibit strong blue emission with a broad peak at 432 nm. Based on the photoluminescence of Ca15(PO4)2(SiO4)6:xCe3+ phosphors, the coordination environment around Ce3+ ions and the concentration quenching mechanism are inferred. With the doping of Tb3+ ions into Ca15(PO4)2(SiO4)6:1.33%Ce3+, the luminescence color from blue to cyan can be well tuned. By measuring the luminescence intensity and lifetime of the as-prepared phosphors, it can be judged that there exists an energy transfer from Ce3+ to Tb3+. To achieve white light, the optimal Ca15(PO4)2(SiO4)6:1.33%Ce3+, 9%Tb3+ phosphors are mixed with commercial SrAlSiN3:Eu2+ powders and finally warm white light emission could be obtained. The results show that Ca15(PO4)2(SiO4)6:xCe3+,yTb3+ phosphors have potential applications in warm white light-emitting diodes.
ABSTRACT
A gate electrode is usually used to controllably tune the carrier concentrations, further modulating the electrical conductivity and the Seebeck coefficient to obtain the optimum thermoelectric figure of merit (ZT) in two-dimensional materials. On the other hand, it is necessary to investigate how an electric field induced by a gate voltage affects the electronic structures, further determining the thermoelectric properties. Therefore, by using density functional calculations in combination with Boltzmann theory, the thermoelectric properties of bilayer MX2 (M = W, Mo; X = S, Se) with or without a 1 V nm-1 perpendicular electric field are comparatively investigated. First of all, the variations of the electrical conductivity (σ), electron thermal conductivity and Seebeck coefficient (S) with the carrier concentration are studied. Due to the trade-off relationship between S and σ, there is an optimum concentration to obtain the maximum ZT, which increases with the temperature due to the enhancement of the Seebeck coefficient. Moreover, N-type bilayers have larger optimum ZTs than P-type bilayers. In addition, the electric field results in the increase of the Seebeck coefficient in low hole-doped MS2 bilayers and high hole-doped MSe2 bilayers, thus leading to similar variations in ZT. The optimum ZTs are reduced from 2.11 × 10-2, 3.19 × 10-2, 2.47 × 10-2, and 2.58 × 10-2 to 1.57 × 10-2, 1.51 × 10-2, 2.08 × 10-2, and 1.43 × 10-2 for the hole-doped MoS2, MoSe2, and WSe2 bilayers, respectively. For N-type bilayers, the electric field shows a destructive effect, resulting in the obvious reduction of the Seebeck coefficient in the MSe2 layers and the low electron-doped MS2 bilayers. In electron-doped bilayers, the optimum ZTs will decrease from 3.03 × 10-2, 6.64 × 10-2, and 6.69 × 10-2 to 2.81 × 10-2, 3.59 × 10-2, and 4.39 × 10-2 for the MoS2, MoSe2, and WSe2 bilayers, respectively.
ABSTRACT
This study is built on density functional calculations in combination with the non-equilibrium Green's function, and we probe the thermoelectric transport mechanisms through C60 molecules anchored to Al nano-electrodes in three different ways, such as, the planar, pyramidal, and asymmetric surfaces. When the electrode is switched from the planar and pyramidal surfaces, the electrical conductance (σ) and electron's thermal conductance (κel) decrease almost two orders of magnitude due to the reduction of the molecule-electrode contact coupling, whereas the Seebeck coefficients (S) are reduced by â¼55%. Furthermore, the maximum electron's thermoelectric figure of merit (ZelT = S2σT/κel, assuming a vanishing phonon's thermal conductance) is about 0.12 in the asymmetric junction. In particular, all σ, S, κel, and ZelT increase along with the average temperature (T) in all C60-junctions, although their growth is really quite negligible in the pyramidal junction because the Fermi level is far away from the frontier orbitals. In addition, when the strain increases from the compressive (-1.0 Å) to tensile (1.0 Å) strain, the Seebeck coefficient in the planar junction increases drastically, while the Seebeck coefficients in the asymmetric and pyramidal junctions reach their maximum values at 0.2 Å tensile and -0.4 Å compressive strains, respectively. This is because the Seebeck coefficient is inversely proportional to the magnitudes and proportional to the slopes of the transmission spectrum around the Fermi level. Finally, it is found that the shift of the Fermi level is an effective scheme to obtain the maximum ZelT of any molecular junction, including fullerene-based junctions.
ABSTRACT
We report enhanced thermoelectric (TE) performance in CdO by using thermally insulating nano-particles to mimic nano pores in the composite. Through simply mechanical alloying, we fabricated CdO-SiO2 composites with varying nano-SiO2 concentration from 0.1 to 3 at%. Due to the very low thermal conductivity of nano-SiO2 distributed in the CdO matrix, the thermal conductivity of the composite was substantially reduced by about 80%, which lead to the dimensionless figure of merit (ZT) value increment about 28% (from 0.32 to 0.41) at 1000 K. TEM shows the nano inclusions formed within the CdO matrix and grain boundaries as well, which is thought to contribute to the reduction of thermal conductivity of the composite by additional scattering mechanism for the mid- to long-wavelength phonons. This facile and low-cost approach might be widely adopted and synergized to other TE materials systems to further improve their performance.
ABSTRACT
RNA editing is a post-transcriptional modification with a cell-specific manner and important biological implications. Although single-cell RNA-seq (scRNA-seq) is an effective method for studying cellular heterogeneity, it is difficult to detect and study RNA editing events from scRNA-seq data because of the low sequencing coverage. To overcome this, we develop a computational method to systematically identify RNA editing sites of cell types from scRNA-seq data. To demonstrate its effectiveness, we apply it to scRNA-seq data of human hematopoietic stem/progenitor cells (HSPCs) with an annotated lineage differentiation relationship according to previous research and study the impacts of RNA editing on hematopoiesis. The dynamic editing patterns reveal the relevance of RNA editing on different HSPCs. For example, four microRNA (miRNA) target sites on 3' UTR of EIF2AK2 are edited across all HSPC populations, which may abolish the miRNA-mediated inhibition of EIF2AK2. Elevated EIF2AK2 may thus activate the integrated stress response (ISR) pathway to initiate global translational attenuation as a protective mechanism to maintain cellular homeostasis during HSPCs' differentiation. Besides, our findings also indicate that RNA editing plays an essential role in the coordination of lineage commitment and self-renewal of hematopoietic stem cells (HSCs). Taken together, we demonstrate the capacity of scRNA-seq data to exploit RNA editing events of cell types, and find that RNA editing may exert multiple modules of regulation in hematopoietic processes.
Subject(s)
MicroRNAs , Single-Cell Gene Expression Analysis , Humans , Single-Cell Analysis/methods , MicroRNAs/genetics , Hematopoiesis/genetics , Cell Differentiation , Sequence Analysis, RNA/methods , 3' Untranslated Regions , Gene Expression Profiling/methodsABSTRACT
BACKGROUND: Hematopoietic stem cells (HSCs) from different sources show varied repopulating capacity, and HSCs lose their stemness after long-time ex vivo culture. A deep understanding of these phenomena may provide helpful insights for HSCs. METHODS: Here, we applied single-cell RNA-seq (scRNA-seq) to analyse the naïve and stimulated human CD34+ cells from cord blood (CB) and mobilised peripheral blood (mPB). RESULTS: We collected over 16 000 high-quality single-cell data to construct a comprehensive inference map and characterised the HSCs under a quiescent state on the hierarchy top. Then, we compared HSCs in CB with those in mPB and HSCs of naïve samples to those of cultured samples, and identified stemness-related genes (SRGs) associated with cell source (CS-SRGs) and culture time (CT-SRGs), respectively. Interestingly, CS-SRGs and CT-SRGs share genes enriched in the signalling pathways such as mRNA catabolic process, translational initiation, ribonucleoprotein complex biogenesis and cotranslational protein targeting to membrane, suggesting dynamic protein translation and processing may be a common requirement for stemness maintenance. Meanwhile, CT-SRGs are enriched in pathways involved in glucocorticoid and corticosteroid response that affect HSCs homing and engraftment. In contrast, CS-SRGs specifically contain genes related to purine and ATP metabolic process, which is crucial for HSC homeostasis in the stress settings. Particularly, when CT-SRGs are used as reference genes for the construction of the development trajectory of CD34+ cells, lymphoid and myeloid lineages are clearly separated after HSCs/MPPs. Finally, we presented an application through a small-scale drug screening using Connectivity Map (CMap) against CT-SRGs. A small molecule, cucurbitacin I, was found to efficiently expand HSCs ex vivo while maintaining its stemness. CONCLUSIONS: Our findings provide new perspectives for understanding HSCs, and the strategy to identify candidate molecules through SRGs may be applicable to study other stem cells.
Subject(s)
Cell Differentiation , Fetal Blood , Hematopoietic Stem Cells , Humans , Antigens, CD34/analysis , Fetal Blood/cytology , Hematopoietic Stem Cells/cytology , Single-Cell Analysis , Gene Expression Profiling , Cell Differentiation/geneticsABSTRACT
Eu2+ doped BaCl(x)Br(2-x), phosphors were prepared by solid state method in the present paper. The crystal structure and luminescent properties were studied by XRD, excitation, emission, and photostimulation. The XRD patterns indicate thatthe samples are single phase of BaCl(x)Br(2-x). The X-ray diffraction peak shifts to larger angle as the value of X increases. The emission spectra is a narrow band with a peak locating at 405 nm, which is attributed to the transition of 4f(6)5d-->4f(7). The excitation spectrum excited by 405 nm is a broad band ranging from 250-380 nm with a peak locating at 303 nm. The photostimulation spectrum is a broad band ranging from 480-800 nm with a peak locating at 575 nm. Through fitting the spectrum curve, the photostimulation spectrum is composed of three bands with peaks locating at about 550, 610 and 685 nm. The three fitting bands correspond to the three color-centers belonging to F(Cl-), F(C1-Br) and F(Br-) centers, respectively. The photostimulation peaks show a blue shift with increasing the ratio of Cl/Br.
ABSTRACT
Placenta plays essential role in successful pregnancy, as the most important organ connecting and interplaying between mother and fetus. However, the cellular characteristics and molecular interaction of cell populations within the fetomaternal interface is still poorly understood. Here, we surveyed the single-cell transcriptomic landscape of human full-term placenta and revealed the heterogeneity of cytotrophoblast cell (CTB) and stromal cell (STR) with the fetal/maternal origin consecutively localized from fetal section (FS), middle section (Mid_S) to maternal section (Mat_S) of maternal-fetal interface. Then, we highlighted a subpopulation of CTB, named trophoblast progenitor-like cells (TPLCs) existed in the full-term placenta and mainly distributed in Mid_S, with high expression of a pool of putative cell surface markers. Further, we revealed the putative key transcription factor PRDM6 that might promote the differentiation of endovascular extravillous trophoblast cells (enEVT) by inhibiting cell proliferation, and down-regulation of PRDM6 might lead to an abnormal enEVT differentiation process in PE. Together, our study offers important resources for better understanding of human placenta and stem cell-based therapy, and provides new insights on the study of tissue heterogeneity, the clinical prevention and control of PE as well as the maternal-fetal interface.
Subject(s)
Fetus , Trophoblasts , Cell Differentiation/genetics , Female , Humans , Placenta/metabolism , Pregnancy , Stem Cells , Trophoblasts/metabolismABSTRACT
Interspecies blastocyst complementation enables organ-specific enrichment of xenogeneic pluripotent stem cell (PSC) derivatives, which raises an intriguing possibility to generate functional human tissues/organs in an animal host. However, differences in embryo development between human and host species may constitute the barrier for efficient chimera formation. Here, to understand these differences we constructed a complete single-cell landscape of early embryonic development of pig, which is considered one of the best host species for human organ generation, and systematically compared its epiblast development with that of human and monkey. Our results identified a developmental coordinate of pluripotency spectrum among pigs, humans and monkeys, and revealed species-specific differences in: (1) pluripotency progression; (2) metabolic transition; (3) epigenetic and transcriptional regulations of pluripotency; (4) cell surface proteins; and (5) trophectoderm development. These differences may prevent proper recognition and communication between donor human cells and host pig embryos, resulting in low integration and survival of human cells. These results offer new insights into evolutionary conserved and divergent processes during mammalian development and may be helpful for developing effective strategies to overcome low human-pig chimerism, thereby enabling the generation of functional human organs in pigs in the future.
ABSTRACT
Human umbilical cord-derived mesenchymal stem/stromal cells (UMSCs) demonstrate great therapeutic potential in regenerative medicine. The use of UMSCs for clinical applications requires high quantity and good quality of cells usually by in vitro expansion. However, the heterogeneity and the characteristics of cultured UMSCs and the cognate human umbilical cord tissue at single-cell resolution remain poorly defined. In this study, we created a single-cell transcriptome profile of human umbilical cord tissue and the cognate culture-expanded UMSCs. Based on the inferred characteristics of cell clusters and trajectory analysis, we identified three subgroups in culture-expanded UMSCs and putative novel transcription factors (TFs) in regulating UMSC state transition. Further, putative ligand-receptor interaction analysis demonstrated that cellular interactions most frequently occurred in epithelial-like cells with other cell groups in umbilical cord tissue. Moreover, we dissected the transcriptomic differences of in vitro and in vivo subgroups and inferred the telomere-related molecules and pathways that might be activated in UMSCs for cell expansion in vitro. Our study provides a comprehensive and integrative study of the transcriptomics of human umbilical cord tissue and their cognate-cultured counterparts, which paves the way for a deeper understanding of cellular heterogeneity and offers fundamental biological insight of UMSCs-based cell therapy.
Subject(s)
Genetic Heterogeneity , Mesenchymal Stem Cells/metabolism , Transcriptome/genetics , Umbilical Cord/metabolism , Cell Differentiation/genetics , Cells, Cultured , Gene Expression Profiling , Gene Expression Regulation/genetics , Humans , Mesenchymal Stem Cell Transplantation , Single-Cell Analysis , Umbilical Cord/cytologyABSTRACT
ß-Thalassemia is one of the most prevalent genetic diseases worldwide. The current treatment for ß-thalassemia is allogeneic hematopoietic stem cell transplantation, which is limited due to lack of matched donors. Gene therapy has been developed as an alternative therapeutic option for transfusion-dependent ß-thalassemia (TDT). However, successful gene therapy for ß-thalassemia patients in China has not been reported. Here, we present the results of preclinical studies of an optimally designed lentiviral vector (LV) named LentiHBBT87Q in hematopoietic stem and progenitor cells (HSPCs) derived from Chinese TDT patients. LentiHBBT87Q was selected from a series of LVs with optimized backbone and de novo cloning strategy. It contains an exogenous T87Q ß-globin gene (HBBT87Q) driven by a specific reconstituted locus control region, and efficiently expresses HBB mRNA and HBB protein in erythroblasts derived from cord blood HSPCs. To facilitate clinical transformation, we manufactured clinical-grade LentiHBBT87Q (cLentiHBBT87Q) and optimized its transduction procedure. Importantly, transduction of cLentiHBBT87Q restored expression of HBB monomer and adult hemoglobin tetramer to relatively normal level in erythroblasts from bone marrow HSPCs of Chinese TDT patients that carry the most common mutation types and cover various genotypes, including ß0/ß0. Furthermore, viral integration sites (VISs) of cLentiHBBT87Q were similar to other LVs safely used in previous clinical trials, and gene-ontology (term) analysis of VIS targeted genes suggests that no tumor-associated pathways were enriched in treated samples. Taken together, we have engineered the cLentiHBBT87Q that can restore ß-globin expression in the HSPCs-derived erythroblasts of Chinese TDT patients with minimal risk of tumorigenesis, providing a favorable starting point for future clinical application.
Subject(s)
beta-Globins , beta-Thalassemia , Genetic Therapy , Genetic Vectors/genetics , Humans , Lentivirus/genetics , beta-Globins/genetics , beta-Thalassemia/genetics , beta-Thalassemia/therapyABSTRACT
ß-thalassemia, caused by mutations in the human hemoglobin ß (HBB) gene, is one of the most common genetic diseases in the world. The HBB -28(A>G) mutation is one of the five most common mutations in Chinese patients with ß-thalassemia. However, few studies have been conducted to understand how this mutation affects the expression of pathogenesis-related genes, including globin genes, due to limited homozygote clinical materials. Therefore, we developed an efficient technique using CRISPR/Cas9 combined with asymmetric single-stranded oligodeoxynucleotides (assODNs) to generate a K562 cell model with HBB -28(A>G) named K562-28(A>G). Then, we systematically analyzed the differences between K562-28(A>G) and K562 at the transcriptome level by high-throughput RNA-seq before and after erythroid differentiation. We found that the HBB -28(A>G) mutation not only disturbed the transcription of HBB, but also decreased the expression of HBG, which may further aggravate the thalassemia phenotype and partially explain the more severe clinical outcome of ß-thalassemia patients with the HBB -28(A>G) mutation. Moreover, we found that the K562-28(A>G) cell line is more sensitive to hypoxia and shows a defective erythrogenic program compared with K562 before differentiation. Importantly, all abovementioned abnormalities in K562-28(A>G) were reversed after correction of this mutation with CRISPR/Cas9 and assODNs, confirming the specificity of these phenotypes. Overall, this is the first time to analyze the effects of the HBB -28(A>G) mutation at the whole-transcriptome level based on isogenic cell lines, providing a landscape for further investigation of the mechanism of ß-thalassemia with the HBB -28(A>G) mutation.
ABSTRACT
Human mesenchymal stem cells (hMSCs) are widely used in clinical research because of their multipotential, immunomodulatory, and reparative properties. Previous studies determined that hMSC spheroids from a three-dimensional (3D) culture possess higher therapeutic efficacy than conventional hMSCs from a monolayer (2D) culture. To date, various 3D culture methods have been developed to form hMSC spheroids but most of them used culture medium containing fetal bovine serum (FBS), which is not suitable for further clinical use. Here, we demonstrate that dissociated single MSCs seeded in induced pluripotent stem medium (MiPS) adhere loosely to the dish and spontaneously migrate to form spheroids during day 3 to day 6. Through component deletion screening and complementation experiments, the knockout serum replacement (KSR) was identified as necessary and sufficient for hMSC spheroid formation. Transcriptome analysis showed that the overall expression profiles were highly similar between 2D culture with FBS and KSR-derived spheroids. Interestingly, genes related to inflammatory response, immune response, and angiogenesis were upregulated in spheroids at day 6 and qPCR results further validated the increased expression level of related genes, including STC1, CCL7, HGF, IL24, and TGFB3. When spheroids were replated in normal FBS medium, cells formed a typical spindle-shaped morphology and FACS results showed that the recovered cells retained MSC-specific surface markers, such as CD73, CD90, and CD105. In summary, we developed a practical and convenient method to generate hMSC spheroids for clinical research and therapy.
ABSTRACT
Integrative analysis of multi-omics layers at single cell level is critical for accurate dissection of cell-to-cell variation within certain cell populations. Here we report scCAT-seq, a technique for simultaneously assaying chromatin accessibility and the transcriptome within the same single cell. We show that the combined single cell signatures enable accurate construction of regulatory relationships between cis-regulatory elements and the target genes at single-cell resolution, providing a new dimension of features that helps direct discovery of regulatory patterns specific to distinct cell identities. Moreover, we generate the first single cell integrated map of chromatin accessibility and transcriptome in early embryos and demonstrate the robustness of scCAT-seq in the precise dissection of master transcription factors in cells of distinct states. The ability to obtain these two layers of omics data will help provide more accurate definitions of "single cell state" and enable the deconvolution of regulatory heterogeneity from complex cell populations.
Subject(s)
Chromatin/genetics , Epigenomics , Gene Expression Regulation , Single-Cell Analysis/methods , Transcriptome , Chromatin/metabolism , Embryo, Mammalian/cytology , Embryo, Mammalian/metabolism , HCT116 Cells , HeLa Cells , Humans , K562 Cells , Regulatory Sequences, Nucleic Acid/genetics , Sequence Analysis, DNA/methodsABSTRACT
Spermatogenic lineage has been directly generated in spermatogonial stem cell (SSC) conditions from human pluripotent stem cells (PSCs). However, it remains unknown whether mouse embryonic stem cells (ESCs) can directly differentiate into advanced male germ cell lineage in the same conditions. Here, we showed rather low efficiency of germ-like cell generation from mouse ESCs in SSC conditions. Interestingly, addition of retinoic acid (RA) into SSC conditions enabled efficient differentiation of mouse ESCs into germ-like cells, as shown by the activation of spermatogenesis-associated genes such as Mvh, Dazl, Prdm14, Stella, Scp1, Scp3, Stra8 and Rec8 In contrast, for cells cultured in control medium, the activation of the above genes barely occurred. In addition, RA with SSC conditions yielded colonies of Acrosin-expressing cells and the positive ratio reached a peak at day 6. Our work thus establishes a simple and cost-efficient approach for male germ like cell differentiation from mouse PSCs and may propose a useful strategy for studying spermatogenesis in vitro.
Subject(s)
Adult Germline Stem Cells/physiology , Germ Cells/physiology , Stem Cells/physiology , Tretinoin/pharmacology , Adult , Animals , Cell Differentiation/physiology , Cells, Cultured , Humans , Male , Mice , Spermatogenesis/physiologyABSTRACT
The photoelectron decay characteristic directly reflects the photographic efficiency of silver halide crystals. Measurement of the electronic decay time-resolved spectrum of silver halide microcrystals can provide important information about the photoelectron decay action in latent image formation process. In order to know the influence of shallow electron trap dopant K4 Fe (CN)6 and S+Au on photoelectron decay, the photoelectron decay time-resolved spectra of AgCl emulsion doped by K4 Fe(CN) and that doped by K4 Fe(CN) firstly and then sensitized by S+Au were detected by microwave absorption dielectric technique, which can be used to study the decay process of free photoelectrons and shallow-trapped electrons in semiconductor crystals. The experimental results show that when the doping content is 10(-8)-10(-7) mol x mol(-1) Ag, the photoelectron decay process becomes slower, namely, the photoelectron decay time is longer, as the doping is near the grain surface before sensitization. After S+Au sensitization, the photoelectron decay becomes faster, showing that the sensitization centre acts as a deep electron trap. And when the doping is near the grain surface with 90% Ag, the photoelectron decay time becomes shorter, showing that the doping centre and the sensitization centre may interact.
ABSTRACT
Molybdenum disulfide (MoS2) nanosheets were synthesized by using a simple heating-up approach, in which 1-dodecanethiol (DDT) was used not only as a sulfur source but also as the surface ligand. The sheet-like morphology was confirmed by the transmission electron microscopy (TEM) and atomic force microscopy (AFM) results, and the X-ray diffraction (XRD) patterns and Raman spectrum were employed to characterize the structure of the as-synthesized MoS2 nanosheets. The as-obtained MoS2 nanosheets blending with a polymer could be used to fabricate an electrically bistable device through a simple spin-coating method, and the device exhibited an obvious electrical bistability in the I-V curve. The charge transport of the device was discussed based on the organic electronic models.