ABSTRACT
With the rapid development of the economy and population, the improvement of life level, enormous organic wastes have been generated. Black soldier fly larvae (BSFL) treatment is an attractive management method as it provides a strategy for waste treatment while also generate biofertilizers. The aim of this study was to evaluate the BSFL processing residue quality through the physical and chemical parameters. The sewage sludge (T1) and food waste (T2) were employed with BSFL (7:1.2 ration on fresh weight basis) and without BSFL T3 and T4 was marked control and composted for 9 days. The results showed that the BSFL composting reduced the organic matter by 14.51-21.98% and the accumulation of volatile fatty acids by 10.12-28.50%. While BSFL composting greatly increased total kjeldahl nitrogen by 23.15% compared with T4, T1 remained essentially unchanged. The additive of BSFL was significantly increased the total phosphorous and potassium in T2, but T1 remained stable compared with control. These results showed that the BSFL could improve the quality of end product and promote the food waste degradation. The current study indicates that the BSFL management provides an environmentally relevant alternative with very convenience in food waste. Further research should focus on residue sanitation.
Subject(s)
Composting , Refuse Disposal , Simuliidae , Animals , Food , Larva , SewageABSTRACT
The increasing number of livestock farms has led to a great deal of manure generation, and its improper treatment results in threats to the environment. Black soldier fly larvae (BSFL) have the potential to effectively convert manure into high-quality fertilizer. The aims of this investigation were to observe the organic matter transformation of different livestock manures and volatile fatty acids (VFAs), and to evaluate the end product quality. Three types of manure [chicken (T1), pig (T2), and cow (T3)] were inoculated with BSFL (1.2:7 ratio on fresh weight basis), three types without BSFL were used as control (T4, T5, and T6), and both were composted for 9 days. The results showed that the BSFL composting reduced the organic matter by 20.31-22.18% and the accumulation of VFAs by 25.58-80.08% as compared to the control. BSFL composting greatly decreased the nitrogen, by 6.08-14.37%. The employment of BSFL significantly increased the total phosphorous (TP), total Kjeldahl nitrogen (TKN), and total nutrients by 42.30-64.16%, 45.41-88.17%, and 26.51-33.34%, respectively. This study showed that employing BSFL could improve the quality of the product and the maturity degree of the composting. Therefore, the BSFL could be added as a high-efficiency transformation agent for converting organic manure into stable compost, especially in developing countries, where adopting technical devices for composting is expensive and difficult to manipulate.
Subject(s)
Composting , Simuliidae , Animals , Cattle , Female , Fertilizers , Larva , Manure , SwineABSTRACT
Rice bran protein (RBP) aggregates were prepared by heating of RBP solution at 90 °C for 4 h at pH 2, 7, or 11 and used for preparing of packaging films. The structure and properties of RBP aggregates and RBP-based films were characterized with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transmission electron microscopy, scanning electron microscope, differential scanning calorimetry, Fourier transform infrared spectroscopy and circular dichroism. The results showed formation of fibrillar, globular, and large molecular protein aggregates during the heating at pH 2, 7 and 11. The heat-aggregated RBP-based films exhibited lower opacity, moisture content, water solubility, and water vapor permeability than those of untreated RBP-based films. Also, improved mechanical and thermal properties were found for the heat-aggregated RBP-based films. In addition, the heat-aggregated RBP-based film at pH 11 showed homogenous and smooth surface as well as compact appearance compared with the untreated RBP-based films or heat-aggregated RBP-based film at pH 2 or 7. Furthermore, the secondary structure of heat-aggregated RBP film exhibited an increase in ß-sheet content and molecular interactions through non-covalent bonds. The obtained results indicated that formation of protein aggregates could improve physical, mechanical, and thermal properties of RBP-based film, especially at pH 11.
ABSTRACT
To increase the anti-digestion ability of extruded rice starch (ERS), the influence of rice glutelin (RG) on digestive and structural characteristics of ERS were investigated. The resistant starch content increased from 4.49 % to 18.08 % as the RG content increased, while the digestion rate and digestion velocity constant were reduced by the incorporation of RG. Morphological observations showed that ERS was adhered and encapsulated by RG, and the specific area of starch granules were decreased after the addition of RG. The results of XRD and FTIR suggested that the long-range and short-range orders of ERS were improved due to the complexation with RG. The thickness of crystalline of ERS was increased while its amorphous region thickness was reduced by the supplementation with RG. The 1H NMR and 13C NMR data revealed that the branching degree and double helix content of ERS was increased by 46.24 % and 52.67 % when RG content reached to 12 %. Additionally, the addition of RG altered the molecular weight and chain length distribution of ERS. The α-amylase activity and glucoamylase activity was inhibited by RG. These results could provide a valuable basis for the application of RG in extruded rice starchy foods with lower glycemic index.
Subject(s)
Oryza , Starch , Starch/chemistry , Oryza/chemistry , Glutens/metabolism , Digestion , Glycemic IndexABSTRACT
This work studied effects of different amounts of rice glutelin (RG) on physicochemical and structural properties of extruded rice starch (ERS) and explored the underlying mechanism of interaction between rice starch and RG upon extrusion processing. The results showed that the addition of RG altered the pasting properties, improved the viscoelastic, and increased the water mobility of ERS. The weight loss of ERS decreased from 71.40 % to 62.61 %, while the degradation temperature increased from 290.48 °C to 296.25 °C as the RG content increased from 0 % to 12 %. The complex index of extruded starch-glutelin complexes significantly elevated from 10.40 % to 35.81 % when RG content increased from 6 % to 12 %. Fourier-transform infrared spectra confirmed that RG interacted with starch via Maillard reactions, and the binding strength between RG and starch was enhanced at a higher RG content. Furthermore, results of rheological property and chemical interactions demonstrated that hydrogen bonding, hydrophobic, and electrostatic interaction were formed between RG and starch during extrusion. In summary, the obtained results of this study can further enrich the theory of starch-protein interactions and show the possibility of RG applied in the extruded starchy foods.
Subject(s)
Oryza , Starch , Starch/chemistry , Glutens/chemistry , Temperature , Hydrogen Bonding , Maillard Reaction , Oryza/chemistryABSTRACT
This study investigated effects of carboxymethylcellulose sodium (CMC) on the conformational evolution of pea protein during the high moisture extrusion process. The morphological observation showed that the addition of CMC facilitated the formation of fibrous structure of pea protein. In comparison with the pea protein in the melting zone and extrudate, the combination of CMC increased the denaturation enthalpy of pea protein by 2.09 % and 2.34 %. Compared with the material in the mixing zone, the degree of grafting between CMC and pea protein in the die was enhanced by 98.95 %. In general, the supplementation of CMC depressed the exposure of hydrophobic groups in the pea protein. In the extrusion barrel, the CMC increased the unfolding of protein molecular chains while it promoted the refolding of protein chains in the die. For the extrudate, the addition of CMC decreased the contents of α-helix and ß-sheet of pea protein by 9.67 % and 6.93 % while the contents of ß-turn and random coil were increased, leading to changes in the molecular weight distribution of protein molecules. In conclusion, these results provided new strategies toward producing the high-quality pea protein-based meat analogues by adding CMC.
ABSTRACT
Reconstituted rice produced by extrusion has been attracted attention due to nutritional fortification and convenient production. Nevertheless, how to achieve desirable qualities and physicochemical properties of reconstituted rice nearly to natural rice by regulating extrusion process parameters is difficult. Herein, rice starch/glutelin mixture as raw material of reconstituted rice was extruded at varying extrusion conditions. Specific mechanical energy (SME) and sectional expansion index (SEI) dropped with rise in density (R2 = 0.9117 and 0.8207). Solubility was enhanced with increase in product temperature (R2 = 0.9085), color darkened and shifted to reddish and yellowish as extrusion temperature increased (R2 = 0.8577). These trends were well fitted by sigmoid models. Furthermore, SME enhanced hydrophobic and electrostatic interactions between rice starch and glutelin and caused the reduction in crystallinity and thermal stability, promoting the formation of a bi-continuous matrix of protein aggregates with rice starch. The obtained results can be applied to guide the production of reconstituted rice with desirable qualities.
Subject(s)
Food Handling , Oryza , Food Handling/methods , Oryza/chemistry , Glutens , Starch/chemistry , TemperatureABSTRACT
Extruded glutelin/rice starch composites were prepared using twin-screw extrusion at various specific mechanical energies (SME), and the interaction mechanism of glutelin and rice starch was investigated at the molecular level. The results indicated that the structure of glutelin was destroyed, hydrophobic interactions and hydrogen bonds between rice starch and glutelin were formed and enhanced as the SME increased, and new hydrogen bonds were formed at the carbonyl (δ- and γ-carbons of glutelin) and C-1 of Tyr. Molecular docking studies confirmed that SME promoted the simultaneous occurrence of the Millard reaction and non-covalent reaction between glutelin and small molecular sugars produced by starch degradation, providing information on binding sites. Additionally, scanning electron microscopy (SEM) revealed dense and uniform flake-like structures induced by these binding interactions. Overall, insights into the interaction mechanism of rice starch and glutelin provide theoretical references for generating reconstituted rice products using extrusion processing.
Subject(s)
Glutens , Oryza , Glutens/chemistry , Oryza/chemistry , Molecular Docking Simulation , Starch/chemistry , Hydrogen BondingABSTRACT
Bacterial communities were dynamically tracked at four stages of biochar-driven sheep manure pile composting, and the co-occurrence networks with keystone taxa were established. The succession of bacterial community obvious varied during the composting process, Proteobacteria predominant in initial stage (39%) then shifted into Firmicutes in thermophilic (41%) and mesophilic (27%) stages, finally the maturation stage dominant by Bacteroidota (26%). Visualizations of bacterial co-occurrence networks demonstrate more cooperative mutualism and complex interactions in the thermophilic and mesophilic phases. Noticeably, the 7.5 and 10% biochar amended composts shown highest connections (736 and 663 total links) and positive cooperation (97.37 and 97.13% positive link) as well as higher closeness centrality and betweenness centrality of keystone taxa. Overall, appropriate biochar addition alters bacterial community succession and strengthens connection between keystone taxa and other bacteria, with 7.5 and 10% biochar amended composts has intense mutualistic symbiosis among bacterial communities.
Subject(s)
Composting , Animals , Sheep , Bacteria , Charcoal , Manure/microbiology , SoilABSTRACT
At present, plant-based simulated meat is attracting more and more attention as a meat substitute. This study discusses the possibility of partial substitution of rice bran (RB) for soybean protein isolate (SPI) in preparing plant-based simulated meat. RB was added to SPI at 0%, 5%, 10%, 15%, and 20% to prepare RB-SPI plant-based simulated meat by the high moisture extrusion technique. RB-SPI plant-based simulated meat revealed greater polyphenol content and preferable antioxidant capacity (DPPH radical scavenging capacity, ABTS scavenging ability, and FRAP antioxidant capacity) compared to SPI plant-based simulated meat. The aromatic amino acids (tryptophan and tyrosine) of RB-SPI plant-based simulated meats tend to be masked first, and then the hydrophobic groups are exposed as RB content increases and the polarity of the surrounding environment increases due to the change in the disulfide conformation of RB-SPI plant-based simulated meats from a stable gauche-gauche-gauche conformation to a trans-gauche-trans conformation.
ABSTRACT
Based on the sustainable development practice-zero growth in chemical fertilizer application, this article used bagasse organic fertilizer and rice husk derived biochar to investigate the response of soil bacterial community in apple orchard. Aimed at realize the soil quality improvement and biomass resource recovery to contribute agricultural and environmental sustainability. The co-trophic Proteobacteria was predominant in all the treatments (29-36 %) and enriched in non-nitrifying Alphaproteobacteria (9-11 %) and ammonia oxidant Betaproteobacteria (8-10 %), especially richest in bagasse fertilizer combine biochar treated soil. In addition, bacterial community variation was assessed by alpha and beta diversity, four treatments dispersed distribution and richer abundance observed in combined apply bagasse fertilizer and biochar treatment (3909.22 observed-species) than single application (3729.88 and 3646.58 observed-species). Biochar as microbial carrier combined organic fertilizer were established synergistic interaction and favorable to organic matter availability during sustainable agriculture. Finally, integrated biochar-bagasse fertilizer was richer than single organic or biochar fertilization in improving soil bacterial diversity, notably by promoting the metabolism of copiotrophic bacteria, nutrient cycling, plant growth and disease inhibit-related bacteria.
Subject(s)
Fertilizers , Malus , Agriculture , Bacteria/genetics , Cellulose , Charcoal , Fertilizers/analysis , Soil , Soil MicrobiologyABSTRACT
Rice Bran (RB) was added to soybean protein isolate (SPI) at 0%, 5%, 10%, 15% and 20% as addition to produce simulated meat by high moisture extrusion, and the apparent properties and structural characteristics of RB-SPI simulated meat were studied. The addition of 10% RB weakened the interaction among hydrogen bond (HB), hydrophobic bond (HI) and disulfide bond (DB), further increasing the hardness of simulated meat. Meanwhile, it decreased the content of intermolecular hydrogen bonding and enhanced the interaction between HI and HB, resulted in an increased tension. Adding 5% RB weakened the interaction between HB, HI and DB, decreased the content of random coils in the secondary structure, but strengthened the DB and ultimately increased the thermal stability of simulated meat.
Subject(s)
Oryza , Soy Foods , Soybean Proteins , Food Handling , HardnessABSTRACT
Aimed to evaluate the coexistence of bacterial and fungal diversity and their correlation with enzymatic activity in response to biochar. This study performed aerobic composting based on typical agricultural wastes of sheep manure with additive apple tree branch biochar at distinct concentration (0, 2.5, 5, 7.5, 10 and 12.5% corresponding from T1 to T6). The result demonstrated that appropriate amendment of biochar enriched bacterial diversity (1646-1686 OTUs) but interestingly decreased fungal diversity (542-630 OTUs) compared to control (1444 and 682 OTUs). Biochar addition enhanced all enzymatic activities and its correlation with bacterial was more complex than fungal community (786 and 359 connect edges). The dominant microbes comprised of Firmicutes (45.2-35.2%), Proteobacteria (14.0-17.5%), Basidiomycota (32.4-49.5%) and Ascomycota (11.3-37.5%) among all the treatments. Overall, biochar regulates the composting microenvironment by influencing the microbial diversity and associated enzymatic activities.
Subject(s)
Composting , Animals , Charcoal , Manure , Sheep , SoilABSTRACT
This study aimed to clarify that long-term leguminous grass mulching (crown vetch (CV) and white clover (WC)) and gramineous grass (orchardgrass (OG)) drive the distribution of soil aggregates and are associated with dissolved organic matter (DOM) components. Excitation emission spectroscopy and parallel factor analysis (EEM-PARAFAC) were used to evaluate the influence of different grass mulches among aggregates. The results indicated that legumes had a more significant impact on the distribution of aggregates and DOM content than gramineae grass mulching. Leguminous grass mulching significantly increased the proportion of macroaggregates >250 µm (74.65%-83.50%) and aggregates associated with dissolved organic carbon (DOC), especially in microaggregates <250 µm (172.27 mg kg-1 to 391.55 mg kg-1). In addition, leguminous grass mulching (CV and WC) contributed more to the increase in soil total nitrogen (TN) and three identified fluorescent components (UVC humic-like, UVA humic-like and protein-like). The component of UVC humic-like relative abundance decreased (48.66%-36.57%), and the protein-like component increased (21.88%-36.50%) as the aggregate size decreased, but the DOM three compositions did not change. The DOM of macroaggregates had higher aromaticity and lower molecular weight than microaggregates, and the highest abundance of UVC humic-like component (54.52%) was found in the gramineous (OG) large macroaggregates, while the higher abundance of protein-like components (31.07%-36.50%) occurs in leguminous mulching (CV and WC) microaggregates. The results contribute to a further understanding of the dynamic process by which grass mulching mediates aggregate formation and DOM component transformation in semiarid apple orchards under grass waste management.
Subject(s)
Carbon , Humic Substances , Factor Analysis, Statistical , Humic Substances/analysis , Poaceae , Soil , Spectrometry, FluorescenceABSTRACT
The grass-waste management model affects soil organic carbon (SOC) and the microorganism community structure; however, studies on the relationship between the fungal community structure and the SOC chemical component at the aggregate level are poor. Solid-state 13C NMR and 18 S rDNA methods were used to evaluate the relationship between the SOC chemical composition and fungal community abundance at the aggregate level. Grass mulching significantly increased the percentage of labile carbon O-alkyl C (5.19%-11.79%) and decreased the instability of SOC (1.38-0.69). Microaggregates contained higher alkyl C (33.77%) and lower aromatic C (18.31%), and the A/O-A ratio (1.03) was higher than that of macroaggregates (0.89-0.96). Ascomycota, Basidiomycota and Mortierellomycota dominated the fungal community at the phylum level, and their abundance increased after grass mulching. Microaggregates supported more microbial diversity and richness and were rich in the Ascomycota (36.69%-67.49%) phylum, while LM aggregates were rich in Basidiomycota (5.62%-39.84%). We proved that changes in the O-alkyl C, carbonyl C, aromatic C and alkyl C of SOC chemical components were closely connected to fungal community composition, which together explained the change in fungal composition by 63.81%-71.99% among aggregates. We concluded that alterations in the chemical form of organic carbon were closely related to a change in the soil fungal community. This connection has a positive impact on soil nutrient utilization and SOC conversion in fruit-grass composite ecosystems and promotes the understanding of the relationship between the soil microbial community and nutrient cycling during long-term grass waste utilization.
Subject(s)
Malus , Microbiota , Mycobiome , Carbon/analysis , China , Poaceae , Soil , Soil MicrobiologyABSTRACT
Blueberry (Vaccinium spp.) is one of the five major healthy foods for humans and is recognized as the "king of the world fruit", which has attracted great interest in the phytogenic prebiotics market. Blueberry fruit is favored for its delicious taste and its various functional ingredients (organic acids, phenolics, minerals and vitamins) with multitherapeutic value (antioxidant, anti-inflammatory, anticancer, neuroprotective and vision improvement properties). However, fresh blueberries are highly perishable since they are vulnerable to mechanical damage and microbial decay, resulting in a short shelf life and inevitable subsequent economic losses. Due to the strong seasonal availability and limited storage period of blueberries, their derived bioactive products have emerged as functional foods. Novel food developments that are currently available include blueberry fruit juice, wine, vinegar, jam, dried fruit, pulp powder, colorant and flavoring additives used in cake, biscuit, bread, yogurt, and jelly. This review systematically describes the current status of blueberry fruit as bioactive ingredients and valuable food products with greater nutraceutical health potential of blueberries.
Subject(s)
Blueberry Plants , Acetic Acid , Antioxidants , Fruit , Humans , Powders , VitaminsABSTRACT
Present study was focus on the pollution control aspect of gaseous mitigation and heavy metal passivation as well as their associated bacterial communities driven by apple tree branch biochar (BB) during sheep manure composting. Six treatment was performed with distinct concentration of BB from 0%, 2.5%, 5%, 7.5%, 10%, and 12.5% as T1 to T6. Compared with compost without additive, biochar-based composting recorded faster thermophilic process (4thd) and longer duration (12-14d), lower gaseous emission in terms of ammonia (5.37-10.29 g), nitrous oxide (0.12-0.47 g) and methane (4.38-30.29 g). Notably highest temperature (65.3 â) and active thermophilic duration (14d), minimized gaseous volatilization were detected in 10%BB composting. Aspect of non-degradability and enrichment-concentration properties of heavy metals, the total copper (Cu) and zinc (Zn) were increased (from initial 12.71-17.91 to final 16.36-29.36 mg/kg and 107.39-146.58-161.48-211.91 mg/kg). In view of available diethylene triamine pentacetic acid (DTPA) extractable form, DTPA-Cu and DTPA-Zn from 4.29 to 6.57 and 31.66-39.32 mg/kg decreased to 3.75-4.82 and 23.43-40.54 mg/kg, especially the maximized passivation rate of 46.95% and 56.27% were present in 10%BB composting. Additionally, bacterial diversity of biochar-based composting was increased (1817-2310 OTUs) than control (1686 OTUs) and dominant by Firmicutes (52.75%), Bacteroidetes (28.41%) and Actinobacteriota (13.98%). Validated 10% biochar-based composting is the optimal option for effectively control environmental pollution to obtain hygienic composting.
Subject(s)
Composting , Metals, Heavy , Animals , Charcoal , Environmental Pollution , Gases , Manure , Metals, Heavy/analysis , Sheep , SoilABSTRACT
In this study, Novozym 435-catalyzed interesterification of ethyl ferulate (EF) with phosphatidylcholine (PC) in a two-phase system consisting of an ionic liquid (IL) and toluene was optimized to prepare feruloylated lysophospholipids (FLPs). Optimum conditions for the interesterification process were found to be [Bmim][Tf2N]/toluene ratio of 1:1 (v/v), solvent volume of 4 mL, molecular sieves (4 Å) concentration of 80 mg/mL, reaction temperature of 55°C, substrate molar ratio of 5:1 (PC/EF), Novozym 435 concentration of 50 mg/mL. Under these conditions, two FLPs products (1-FLP and 2-FLP) with total conversion rate of 50.79% were obtained. Because the formation of 1-FLP was significantly higher than 2-FLP, 1-FLP was purified and characterized by LC-MS and NMR. In addition, 1-FLP showed DPPH scavenging activity comparable with those of EF and BHT. Therefore, this study provides a good method for transformation of ferulic acid to improve its solubility and promote its application as functional ingredient in the food and pharmaceutical industries.
Subject(s)
Antioxidants , Caffeic Acids/chemistry , Ionic Liquids/chemistry , Lipase/chemistry , Lysophospholipids/chemical synthesis , Phosphatidylcholines/chemistry , Toluene/chemistry , Catalysis , Drug Industry , Enzymes, Immobilized , Esterification , Food Industry , Fungal Proteins , Lysophospholipids/chemistry , Lysophospholipids/pharmacology , Solubility , Solvents/chemistry , TemperatureABSTRACT
Present study evaluated the utilization of clean technology for biochar combined with organic fertilizer in apple orchard aspect of soil organic carbon fractions and microbial community. Four treatments were performed with control (CK), rice husk biochar alone (B), bagasse fermented organic fertilizer alone (O) and biochar combined with organic fertilizer (BO). The results demonstrated that utilization of organic fertilizer integrated with biochar were obviously enhanced the total and active fractions organic carbon in the top-soil (0-20 cm), enriched the bacterial community diversity and the richest abundance presented in BO treatment with 4253 operational taxonomic unit. The visualization illustrated the superior bacterial community was affiliated with Proteobacteria (35.14%), Actinobacteria (21.34%), Acidobacteria (16.82%) and Firmicutes (14.70%). Additionally, redundancy analysis suggested the strong interaction between microorganisms and organic carbon fractions. Overall, the application of biochar combine with organic fertilizer was favorable approach in apple orchard management, attributed to the influence of essential factors by improve organic carbon and bacterial diversity especially conductive to the profitable strain proliferation.
Subject(s)
Malus , Soil , Carbon , Charcoal , Fertilizers/analysis , Soil Microbiology , TechnologyABSTRACT
Huge quantities of apple orchard waste (AOW) generated could be regarded as a promising alternative energy source for fuel and material production. Conventional and traditional processes for disposal of these wastes are neither economical nor environment friendly. Hence, sustainable technologies are required to be developed to solve this long-term existence and continuous growing problem. In light of these issues, this review pays attention towards sustainable and renewable systems, various value-added products from an economic and environmental perspective. Refined bio-product derived from AOW contributes to resource and energy demand comprising of biomethane, bioethanol, biofuels, bio-fertilizers, biochar, and biochemicals, such as organic acid, and enzymes. However, the market implementation of biological recovery requires reliable process technology integrated with an eco-friendly and economic production chain, classified management.