ABSTRACT
A simple immunosensor based on a conductivity method was developed for determination of methamphetamine (MA, a stimulant drug) in urine. Anti-MA antibody was immobilized onto the surface of a pair of platinum electrodes. The reaction of MA with the antibody causes a decrease in the conductivity of the anti-MA immobilized layer between the electrodes. A linear relationship was obtained between the conductivity and MA concentration in the range of 1-10 micrograms/ml. The method requires the sample to be rinsed with water on the electrodes after the immunoreaction. This detection system was applied to the determination of MA in urine and proved to be a useful and a simple detection technique of MA in forensic science in comparison with a gas chromatography-mass spectrometry method.
Subject(s)
Biosensing Techniques , Central Nervous System Stimulants/urine , Methamphetamine/urine , Calibration , Electric Conductivity , Humans , Sensitivity and SpecificityABSTRACT
A continuous monitoring system for cyanide with a galvanic hydrogen cyanide sensor and an aeration pump for purging was developed. Hydrogen cyanide evolved from cyanide solution using a purging pump was measured with the hydrogen cyanide sensor. The system showed good performance in terms of stability and selectivity. A linear calibration curve was obtained in the concentrating range from 0 to 15 mg dm(3) of cyanide ion with a slope of -0.24 microA mg(-1) dm(-3). The lower detection limit was 0.1 mg dm(-3). The 90% response time of the sensor system was within 3.5 min for a 0.5 mg dm(-3) cyanide solution, when the flow rate of the purging air was 1 dm(3) min(-1). The system maintained the initial performance for 6 months in the field test. The developed galvanic sensor system was not subject to interference from sulfide and residual chlorine, compared with a potentiometric sensor system developed previously. The analytical results obtained by the present system were in good agreement with those obtained by the pyridine pyrazolone method. The correlation factor and regression line between both methods were 0.979 and Y=2.30 x 10(-4)+1.12X, respectively. This system was successfully applied for a continuous monitoring of cyanide ion in waste water.
ABSTRACT
A simple flow injection analysis (FIA) system for residual chlorine in tap water has been developed by using a Pb(II) ion-selective electrode (ISE) detector. The method is based on a specific response of the Pb(II)-ISE to residual chlorine. The FIA system consists of a millivolt meter, a peristaltic pump, a Pb(II)-ISE detector and a recorder. A linear working curve between peak height and concentration of residual chlorine was obtained from 0.1 to 1 mg l(-1) for the developed FIA system. The relative standard deviation for repeated injections of a 0.2 mg l(-1) residual chlorine sample was 2%. The regression line and its correlation factor between the conventional o-tolidine colorimetric method and the present method were Y=0.75X+0.17 and 0.967, respectively, for this determination.