Effects of Compound Trace Minerals on the Growth Performance, Carcass Characteristics and Meat Quality of Fattening Pigs.

The objective of this study was to evaluate the effects of supplemental levels of compound minerals (CM), which was formulated from a mixture of 300 mg calcium, 150 mg magnesium, 25 mg zinc, 15 mg iron, 4 mg manganese, and 2 mg copper per gram, on the growth performance and feed conversion ratio of Duroc × (Landrace × Yorkshire) fattening pigs. A total of 53 female pigs and 45 male pigs at 35 days of age were individually notched and randomly allocated to three treatments, including0 (basal diet), 0.5 (basal diet with 0.5 mg CM/kg feed), and 1 (basal diet with 1 mg CM/kg feed). There were three replicates for each of the three treatments with 28, 34, and 36 pigs in the first, second, and third treatments, respectively. The experiment was divided into two phases, the growing stage, and the finishing stage. Supplementation of compound minerals in the diet led to an increased average daily weight gain and improved feed conversion ratio and meat quality without effects on the lean meat percentage. An addition of 1 mg of compound minerals per 1 kg of feed is the recommended dose to improve the production performance of fattening pigs. These results suggest that the use of compound minerals could improve the growth traits of fattening pigs under tropical environmental conditions.

Biomarker and proteome analysis of milk from dairy cows with clinical mastitis: Determining the effect of different bacterial pathogens on the response to infection.

Antimicrobial usage (AMU) could be reduced by differentiating the causative bacteria in cases of clinical mastitis (CM) as either Gram-positive or Gram-negative bacteria or identifying whether the case is culture-negative (no growth, NG) mastitis. Immunoassays for biomarker analysis and a Tandem Mass Tag (TMT) proteomic investigation were employed to identify differences between samples of milk from cows with CM caused by different bacteria. A total of 94 milk samples were collected from cows diagnosed with CM across seven farms in Scotland, categorized by severity as mild (score 1), moderate (score 2), or severe (score 3). Bovine haptoglobin (Hp), milk amyloid A (MAA), C-reactive protein (CRP), lactoferrin (LF), α-lactalbumin (LA) and cathelicidin (CATHL) were significantly higher in milk from cows with CM, regardless of culture results, than in milk from healthy cows (all P-values <0.001). Milk cathelicidin (CATHL) was evaluated using a novel ELISA technique that utilises an antibody to a peptide sequence of SSEANLYRLLELD (aa49-61) common to CATHL 1-7 isoforms. A classification tree was fitted on the six biomarkers to predict Gram-positive bacteria within mastitis severity scores 1 or 2, revealing that compared to the rest of the samples, Gram-positive samples were associated with CRP < 9.5 µg/ml and LF ≥ 325 µg/ml and MAA < 16 µg/ml. Sensitivity of the tree model was 64%, the specificity was 91%, and the overall misclassification rate was 18%. The area under the ROC curve for this tree model was 0.836 (95% bootstrap confidence interval: 0.742; 0.917). TMT proteomic analysis revealed little difference between the groups in protein abundance when the three groups (Gram-positive, Gram-negative and no growth) were compared, however when each group was compared against the entirety of the remaining samples, 28 differentially abundant protein were identified including ß-lactoglobulin and ribonuclease. Whilst further research is required to draw together and refine a suitable biomarker panel and diagnostic algorithm for differentiating Gram- positive/negative and NG CM, these results have highlighted a potential panel and diagnostic decision tree. Host-derived milk biomarkers offer significant potential to refine and reduce AMU and circumvent the many challenges associated with microbiological culture, both within the lab and on the farm, while providing the added benefit of reducing turnaround time from 14 to 16 h of microbiological culture to just 15 min with a lateral flow device (LFD).

Effects of Stocking Density in Group Cages on Egg Production, Profitability, and Aggressive Pecking of Hens.

There is an increasing concern about welfare issues related to battery cages, which are commonly used in Vietnam, and requires a modified cage that improves hen welfare while retaining its economic and management advantages. We combined adjacent conventional cages to form group cages to examine the effects of stocking density on egg production, economic returns, and aggressive pecking of hens. The control group included triplicate conventional cages with four birds/single cage (12 hens per three cages) or 450 cm2 area per hen. Three group cage treatments were set up with 10, 12, and 14 birds per group cage or 540, 450, and 386 cm2 of floor area per hen, respectively. Compared to 14 birds per cage, hens housed at 10 birds per group cage had a higher hen-day production, consumed less feed, and thus had a better feed conversion ratio/dozen eggs. Reducing the stocking density to 10 birds per group cage resulted in additional production cost, but it was compensated for by a high egg income, and significantly decreased aggressive pecks. Group cages benefit hen performance, profitability, and welfare when decreasing the stocking density to 10 birds per cage with 540 cm2/hen.

Quantitative proteomics of cerebrospinal fluid using tandem mass tags in dogs with recurrent epileptic seizures.

This prospective study included four dog groups (group A: healthy dogs, groups B: dogs with idiopathic epilepsy under antiepileptic medication (AEM), C: idiopathic epilepsy dogs without AEM administration, D: dogs with structural epilepsy). The purpose of the study was to compare the proteomic profile among the four groups. Samples were analyzed by a quantitative Tandem Mass Tags approach using a Q-Exactive-Plus mass-spectrometer. Identification and relative quantification were performed using Proteome Discoverer, and data were analyzed using R. Gene ontology terms were analyzed based on Canis lupus familiaris database. Data are available via ProteomeXchange with identifier PXD018893. Eighteen proteins were statistically significant among the four groups (P < 0.05). MMP2 and EFEMP2 appeared down-regulated whereas HP and APO-A1 were up-regulated (groups B, D). CLEC3B and PEBP4 were up-regulated whereas APO-A1 was down-regulated (group C). IGLL1 was down-regulated (groups B, C) and up-regulated (group D). EFEMP2 was the only protein detected among the four groups and PEBP4 was significantly different among the epileptic dogs. Western blot and SPARCL immunoassay were used to quantify HP abundance change, validating proteomic analysis. Both, showed good correlation with HP levels identified through proteomic analysis (r = 0.712 and r = 0.703, respectively). SIGNIFICANCE: The proteomic analysis from CSF of dogs with epileptic seizures could reflect that MMP2, HP and APO-A1 may contribute to a blood-brain barrier disruption through the seizure-induced inflammatory process in the brain. MMP2 change may indicate the activation of protective mechanisms within the brain tissue. Antiepileptic medication could influence several cellular responses and alter the CSF proteome composition.