ABSTRACT
HBsAg gene was previously introduced into cherry tomato (Lycopersicum esculentum var. cerasiforme) by Agrobacterium-mediated transformation. To investigate the side effect of HBsAg gene in cherry tomato, we analyzed morphological and physiological characteristics of the transgenic mutant N244. The process was performed under field conditions. The results suggested that the mutant N244 exhibited morphological, cytological and physiological variation. First of all, compared with the wild plants NK, N244 had fleshy and dark green leaves, the fewer notches of leaf edge, more adventitious roots and barren seeds. Moreover, the chromosome of N244 were found to be triploid (n = 36) by flow cytometric analysis. Furthermore, N244 has obvious physiological alterations, as compared to NK. It was speculated that transformation of the genes probably led to ploidy variation, and further caused phenotype and physiological changes of plants. Our study will reveal side effects of the mutants, and promote cultivation of transgenic plants in the field.
Subject(s)
Hepatitis B Surface Antigens/biosynthesis , Mutation , Plants, Genetically Modified/growth & development , Solanum lycopersicum/growth & development , Hepatitis B Surface Antigens/genetics , Solanum lycopersicum/anatomy & histology , Solanum lycopersicum/genetics , Plant Leaves/anatomy & histology , Plant Leaves/genetics , Plant Leaves/growth & development , Plant Roots/anatomy & histology , Plant Roots/genetics , Plant Roots/growth & development , Plants, Genetically Modified/anatomy & histology , Plants, Genetically Modified/genetics , Ploidies , Seeds/anatomy & histology , Seeds/genetics , Seeds/growth & developmentABSTRACT
Gabor schemes of image representation have shown great success in many texture related computer vision applications. This is mainly because the primitives of image representation in vision have a wavelet form similar to Gabor elementary functions (GEF's). We use Gabor approach to analyze the texture of liver CT-images and extract the corresponding feature vectors. Then, the feature vectors are used to facilitate content-based image retrieval (CBIR). In experiments, a batch of liver CT images containing several types of CT findings is collected, and retrieval results by Gabor texture are present.