ABSTRACT
On our ongoing searching for bioactive natural products derived from entophytes, two polyketides possessing novel skeletons, alternatones A-B (1-2), were identified from the culture of Alternaria alternate L-10. Their structures were established by a combination of spectroscopic and single-crystal X-ray diffraction with Cu Ka radiation. Alternatone A (1) exhibited cytotoxic activity against human hepatoma carcinoma HepG-2 cell line. The putative biosynthetic pathways for compounds 1-2 were also proposed.
Subject(s)
Antineoplastic Agents , Polyketides , Alternaria/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Molecular Structure , Polyketides/chemistry , Polyketides/pharmacology , SkeletonABSTRACT
Marine actinomycetes are prolific chemical sources of complex and novel natural products, providing an excellent chance for new drug discovery. The chemical investigation of the marine-derived Streptomyces sp. ITBB-ZKa6, from Zhaoshu island, Hainan, led to the discovery of two unique antimycin-type depsipeptides, zhaoshumycins A (1) and B (2), along with the isolation of the four known neoantimycins A (3), F (4), D (5), and E (6). The structures of the new compounds 1 and 2 were elucidated on the basis of the analysis of diverse spectroscopic data and biogenetic consideration. Zhaoshumycins A (1) and B (2) represent a new class of depsipeptides, featuring two neoantimycin monomers (only neoantimycin D or neoantimycins D and E) linked to a 1,4-disubstituted benzene ring via an imino group. Initial toxicity tests of 1-6 in MCF7 human breast cancer cells revealed that compounds 5 and 6 possess weak cytotoxic activity. Further structure-activity relationship analysis suggested the importance of the NH2 group at C-34 in 5 and 6 for cytotoxicity in MCF7 cells.
Subject(s)
Antimycin A , Antineoplastic Agents , Depsipeptides , Streptomyces , Animals , Humans , Antimycin A/analogs & derivatives , Antimycin A/chemistry , Antimycin A/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Aquatic Organisms , Cell Line, Tumor/drug effects , Depsipeptides/chemistry , Depsipeptides/pharmacology , Structure-Activity RelationshipABSTRACT
Six new macrolides named myrothecines D-G (1-4), 16-hydroxymytoxin B (5), and 14'-dehydrovertisporin (6), including four 10,13-cyclotrichothecane derivatives, in addition to 12 known compounds (7-18), were isolated from three endophytic Myrothecium roridum, IFB-E008, IFB-E009, and IFB-E012. The isolated compounds were characterized by MS, NMR, CD, and single-crystal X-ray crystallography. The isolated macrolides exhibited an antiproliferation effect against chronic myeloid leukemia K562 and colorectal carcinoma SW1116 cell lines. Compounds 1-6 were cytotoxic, with IC50 values ranging between 56 nM and 16 µM. Since slight structural changes led to obvious activity differences, the CoMFA (comparative molecular field analysis) and CoMSIA (comparative molecular similarity indices analysis) methods were then used to explore the 3D QSAR (three-dimensional quantitative structure-activity relationship) of these macrolides. The result showed that the steric, electrostatic, hydrophobic, and H-bond acceptor factors were involved in their cytotoxicity and provided an in-depth understanding of the structure-activity relationships of these metabolites.
Subject(s)
Anti-Bacterial Agents/pharmacology , Antineoplastic Agents/pharmacology , Hypocreales/chemistry , Macrolides/pharmacology , Mitosporic Fungi/chemistry , Protein Synthesis Inhibitors/pharmacology , Trichothecenes/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/isolation & purification , Antineoplastic Agents/chemistry , Antineoplastic Agents/isolation & purification , Crystallography, X-Ray , Macrolides/chemistry , Macrolides/isolation & purification , Magnetic Resonance Spectroscopy , Molecular Structure , Protein Synthesis Inhibitors/chemistry , Protein Synthesis Inhibitors/isolation & purification , Quantitative Structure-Activity Relationship , Trichothecenes/chemistry , Trichothecenes/isolation & purificationABSTRACT
Marine fungi are a promising source of novel bioactive natural products with diverse structure. In our search for new bioactive natural products from marine fungi, three new phenone derivatives, asperphenone A-C (1-3), have been isolated from the ethyl acetate extract of the fermentation broth of the mangrove-derived fungus, Aspergillus sp. YHZ-1. The chemical structures of these natural products were elucidated on the basis of mass spectrometry, one- and two-dimensional NMR spectroscopic analysis and asperphenone A and B were confirmed by single-crystal X-ray crystallography. Compounds 1 and 2 exhibited weak antibacterial activity against four Gram-positive bacteria, Staphylococcus aureus CMCC(B) 26003, Streptococcus pyogenes ATCC19615, Bacillus subtilis CICC 10283 and Micrococcus luteus, with the MIC values higher than 32.0 µM.
Subject(s)
Anti-Bacterial Agents/pharmacology , Aspergillus/metabolism , Benzene Derivatives/pharmacology , Rhizophoraceae/microbiology , Anti-Bacterial Agents/isolation & purification , Aspergillus/isolation & purification , Benzene Derivatives/isolation & purification , Gram-Positive Bacteria/drug effects , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Models, Molecular , Molecular Structure , X-Ray DiffractionABSTRACT
Chaetospirolactone (1), a novel spiro-lactone bearing a rare 1-oxaspiro [4.4] non-7-ene-2,6-dione skeleton, and orsellide F (2), together with six known compounds (3-8), were isolated from an endophytic fungus Chaetomium sp. NF00754. Their structures were determined by interpretation of spectroscopic data. The absolute configurations of 1 and 2 were established by analysis of single X-ray crystallographic data and CD spectra. Compounds 3, 4, and 6 showed moderate acetylcholinesterase inhibitory activity with IC50 values of 7.34, 5.19, and 7.67 µM, respectively.
Subject(s)
Chaetomium/chemistry , Cholinesterase Inhibitors/isolation & purification , Cholinesterase Inhibitors/pharmacology , Lactones/isolation & purification , Resorcinols/isolation & purification , Spiro Compounds/isolation & purification , Acetylcholinesterase/drug effects , Cholinesterase Inhibitors/chemistry , Crystallography, X-Ray , Lactones/chemistry , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Resorcinols/chemistry , Spiro Compounds/chemistryABSTRACT
Five new p-terphenyls named prenylterphenyllin D (1), prenylterphenyllin E (2), 2'-O-methylprenylterphenyllin (3), 4-O-methylprenylterphenyllin (4) and 3'-O-methylterphenyllin (5) together with seven known compounds (6-12), were isolated from cultures of Aspergillus sp. YXf3. The structures of the new compounds were elucidated by extensive MS and NMR analyses. The NMR and MS data of 5 is reported for the first time, as its structure was listed in SciFinder Scholar with no associated reference. Compounds 6 and 7 were distinguished from each other on the basis of 2D NMR experiments. Compounds 1, 2, 3 and 8 showed antibacterial activities against X. oryzae pv. oryzicola Swings and E. amylovora with the same MIC values of 20µg/mL while 10 exhibited activities against E. amylovora with an MIC value of 10µg/mL.
Subject(s)
Anti-Bacterial Agents/pharmacology , Aspergillus/chemistry , Erwinia amylovora/drug effects , Terphenyl Compounds/pharmacology , Xanthomonas/drug effects , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/isolation & purification , Dose-Response Relationship, Drug , Microbial Sensitivity Tests , Molecular Structure , Structure-Activity Relationship , Terphenyl Compounds/chemistry , Terphenyl Compounds/isolation & purificationABSTRACT
Two new alkaloids, strepchazolins A (1) and B (2), together with a previously reported compound, streptazolin (3), were isolated from a marine actinomycete, Streptomyces chartreusis NA02069, collected in the Coast of Hainan Island, China. The structures of new compounds were determined by extensive NMR, mass spectroscopic and X-ray crystallographic analysis, as well as modified Mosher's method. Compound 1 showed weak anti-Bacillus subtilis activity with the MIC value of 64.0 µM, and weak inhibitory activity against acetylcholinesterase (AChE) in vitro with IC50 value of 50.6 µM, while its diastereoisomer, Compound 2, is almost inactive.
Subject(s)
Alkaloids/isolation & purification , Anti-Bacterial Agents/isolation & purification , Streptomyces/chemistry , Acetylcholinesterase/metabolism , Alkaloids/chemistry , Alkaloids/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Bacillus subtilis/drug effects , China , Cholinesterase Inhibitors/pharmacology , Crystallography, X-Ray , Inhibitory Concentration 50 , Marine Biology , Microbial Sensitivity Tests , Molecular Conformation , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Piperidines/chemistry , Piperidines/isolation & purification , StereoisomerismABSTRACT
Prompted by the pressing necessity to conquer phytopathogenic infections, the antimicrobial compounds were characterized with bioassay-guided method from the ethanol extract derived from the solid-substrate fermentation of Aspergillus sp. IFB-YXS, an endophytic fungus residing in the apparently healthy leave of Ginkgo biloba L. The aim of this work was to evaluate the antimicrobial activity and mechanism(s) of these bioactive compounds against phytopathogens. Among the compounds, xanthoascin (1) is significantly inhibitory on the growth of the phytopathogenic bacterium Clavibacter michiganense subsp. Sepedonicus with a minimum inhibitory concentration (MIC) value of 0.31µg/ml, which is more potent than streptomycin (MIC 0.62µg/ml), an antimicrobial drug co-assayed herein as a positive reference. Moreover, terphenyl derivatives 3, 5 and 6 are also found to be active against other phytopathogens including Xanthomonas oryzae pv. oryzae Swings, Xanthomonas oryzae pv. oryzicola Swings, Erwinia amylovora and Pseudomonas syringae pv. lachrymans etc. The antibacterial mechanism of xanthoascin (1) was addressed to change the cellular permeability of the phytopathogens, leading to the remarkable leakage of nucleic acids out of the cytomembrane. The work highlights the possibility that xanthoascin (1), an analogue of xanthocillin which is used to be an approved antibiotic, may find its renewed application as a potent antibacterial agrichemical. This study contributes to the development of new antimicrobial drugs, especially against C. michiganense subsp. Sepedonicus.
Subject(s)
Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Aspergillus/chemistry , Phenols/chemistry , Phenols/pharmacology , Actinobacteria/drug effects , Agrochemicals/chemistry , Agrochemicals/isolation & purification , Agrochemicals/pharmacology , Anti-Infective Agents/isolation & purification , Aspergillus/isolation & purification , Butadienes/chemistry , Butadienes/pharmacology , Drug Discovery , Endophytes/chemistry , Endophytes/isolation & purification , Fermentation , Ginkgo biloba/microbiology , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Phenols/isolation & purification , Plant Diseases/microbiology , Plants/microbiology , Structure-Activity RelationshipABSTRACT
In the present study, the effect of Fumigaclavine C, a fungal metabolite, on murine experimental colitis induced by dextran sulfate sodium (DSS) and its possible mechanism were examined in vivo and vitro. Oral administration of Fumigaclavine C dose-dependently attenuated the loss of body weight and shortening of colon length induced by DSS. The disease activity index, histopathologic scores of musco was also significantly reduced by Fumigaclavine C treatment. Protein and mRNA levels of DSS-induced pro-inflammatory cytokines in colon, including TNF-α, IL-1ß and IL-17A, were markedly suppressed by Fumigaclavine C. At the same time, decreased activation of caspase-1 in peritoneal macrophages was detected in Fumigaclavine C -treated mice which suggested that the NLRP3 inflammasome activation was suppressed. Furthermore, in the LPS plus ATP cell model, we found that Fumigaclavine C dose-dependent inhibited IL-1ß release and caspase-1 activation. Taken together, our results demonstrate the ability of Fumigaclavine C to inhibit NLRP3 inflammasome activation and give some evidence for its potential use in the treatment of inflammatory bowel diseases.
Subject(s)
Carrier Proteins/antagonists & inhibitors , Colitis, Ulcerative/chemically induced , Colitis, Ulcerative/drug therapy , Ergot Alkaloids/pharmacology , Ergot Alkaloids/therapeutic use , Indole Alkaloids/pharmacology , Indole Alkaloids/therapeutic use , Inflammasomes/antagonists & inhibitors , Interleukin-1beta/metabolism , Administration, Oral , Animals , Caspase 1/metabolism , Cells, Cultured , Colon/drug effects , Dextran Sulfate , Dose-Response Relationship, Drug , Ergot Alkaloids/administration & dosage , Female , Humans , Indole Alkaloids/administration & dosage , Interleukin-17/metabolism , Macrophages, Peritoneal/metabolism , Mice, Inbred C57BL , NLR Family, Pyrin Domain-Containing 3 Protein , Tumor Necrosis Factor-alpha/metabolism , Weight Loss/drug effectsABSTRACT
Three new polyketides, named daldinone F (1), nodulisporin G (2), and dalmanol C (3), together with five known compounds, 4-8, were isolated from cultures of Daldinia eschscholzii. The structures of the new compounds were elucidated by extensive NMR and MS analyses. Compound 1 showed moderate cytotoxic activity against SW480 cancer cells with an IC50 value of 9.59â µM, and its absolute configuration was determined by single crystal X-ray diffraction.
Subject(s)
Polyketides/analysis , Xylariales/chemistry , Crystallography, X-Ray , Magnetic Resonance Spectroscopy , Models, MolecularABSTRACT
Three new drimane sesquiterpenoids, astellolides C-E (1-3, resp.), four new drimane sesquiterpenoid p-hydroxybenzoates, astellolides F-I (4-7, resp.), together with two known compounds astellolides A and B (8 and 9, resp.), have been isolated from the liquid culture of Aspergillus oryzae (strain No.â QXPC-4). Their structures were established by comprehensive analysis of spectroscopic data. The relative and absolute configurations were determined on the basis of NOESY and CD data, together with single-crystal X-ray diffraction analyses of compounds 1-3. The metabolites were evaluated for their cytotoxic activities, however, no compounds showed a significant cytotoxicity against the tested cell lines at a concentration of 20â µM.
Subject(s)
Aspergillus oryzae/chemistry , Sesquiterpenes/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/pharmacology , Cell Line, Tumor , Crystallography, X-Ray , Humans , Models, Molecular , Neoplasms/drug therapy , Polycyclic Sesquiterpenes , Sesquiterpenes/isolation & purification , Sesquiterpenes/pharmacologyABSTRACT
Two new tricycloalternarenes I (1) and J (2), together with five known derivatives (3-7), were isolated from the culture of marine fungus Alternaria sp. The structures were elucidated by a combination of spectroscopic approach ((1)H, (13)C NMR, HMBC, COSY, and NOESY) and the low-temperature (100 K) single-crystal X-ray crystallography analysis. The antimicrobial assays of tricycloalternarenes I (1) and J (2) were tested.
Subject(s)
Alternaria/chemistry , Anti-Bacterial Agents/isolation & purification , Terpenes/isolation & purification , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Bacillus subtilis/drug effects , China , Crystallography, X-Ray , Marine Biology , Microbial Sensitivity Tests , Molecular Structure , Mycobacterium smegmatis/drug effects , Nuclear Magnetic Resonance, Biomolecular , Streptococcus pyogenes/drug effects , Terpenes/chemistry , Terpenes/pharmacologyABSTRACT
A series of novel 4,5-dihydropyrazole derivatives (4a-4t), containing the dinitrobenzotrifluoride moiety, as DNA gyrase inhibitors were designed and synthesized. Based on the preliminary results, compounds 4d, 4f and 4t with potent inhibitory activity in bacterial growth may be wonderful antibacterial agents; among them, compound 4t displayed the most potent activity with minimum inhibitory concentration (MIC) values of 3.125, 0.39, 0.39 and 0.39 µg mL(-1) against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli respectively, which was comparable with penicillin and kanamycin B with corresponding MIC values of 3.125, 3.125, 0.39, 0.39 µg mL(-1) and 1.562, 1.562, 1.562, 1.562 µg mL(-1), respectively. In particular, compound 4d showed the most potent anti-Gram-positive bacterial activity with a MIC value of 0.39 µg mL(-1) against the tested Gram-positive bacterial strains and exhibited the most potent B. subtilis DNA gyrase and S. aureus DNA gyrase inhibitory activity with an IC50 of 0.125 µg mL(-1). Docking simulation was performed to insert compound 4d into the S. aureus DNA gyrase active site to determine the probable binding conformation.
Subject(s)
Anti-Bacterial Agents/pharmacology , DNA Gyrase/metabolism , Phenyl Ethers/pharmacology , Pyrazoles/pharmacology , Topoisomerase II Inhibitors/pharmacology , Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/chemistry , Bacillus subtilis/drug effects , Bacillus subtilis/enzymology , Dose-Response Relationship, Drug , Escherichia coli/drug effects , Microbial Sensitivity Tests , Models, Molecular , Molecular Structure , Phenyl Ethers/chemical synthesis , Phenyl Ethers/chemistry , Pseudomonas aeruginosa/drug effects , Pyrazoles/chemical synthesis , Pyrazoles/chemistry , Staphylococcus aureus/drug effects , Staphylococcus aureus/enzymology , Structure-Activity Relationship , Topoisomerase II Inhibitors/chemical synthesis , Topoisomerase II Inhibitors/chemistryABSTRACT
Five new flavonoids, cryptoconones A-E (1-5), along with six known compounds (6-11), were isolated from the stems of Cryptocarya concinna. The structures of these compounds were elucidated on the basis of spectroscopic data interpretation, and the absolute configurations were determined via circular dichroism spectra and X-ray crystal analysis. The cytotoxic and antimicrobial activities of these compounds were also evaluated. Compounds 9 and 10 exhibited moderate cytotoxic activities against HCT116, HT-29, SW480, and MDA-MB-231 cell lines with IC50 values ranging from 6.25 to 9.35 µM. Compounds 8 and 11 exhibited antimicrobial activity against Fusarium moniliforme and Botrytis cinerea, respectively, with the same minimum inhibitory concentration of 5 µg/mL.
Subject(s)
Anti-Infective Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Cryptocarya/chemistry , Flavonoids/pharmacology , Anti-Infective Agents/chemistry , Anti-Infective Agents/isolation & purification , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Botrytis/drug effects , Cell Line, Tumor , Cell Survival/drug effects , Crystallography, X-Ray , Drug Screening Assays, Antitumor , Flavonoids/chemistry , Flavonoids/isolation & purification , Fusarium/drug effects , Humans , Inhibitory Concentration 50 , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Molecular Structure , Plant Stems/chemistryABSTRACT
Peptides are a particular molecule class with inherent attributes of some small-molecule drugs and macromolecular biologics, thereby inspiring continuous searches for peptides with therapeutic and/or agrochemical potentials. However, the success rate is decreasing, presumably because many interesting but less-abundant peptides are so scarce or labile that they are likely 'overlooked' during the characterization effort. Here, we present the biochemical characterization and druggability improvement of an unprecedented minor fungal RiPP (ribosomally synthesized and post-translationally modified peptide), named acalitide, by taking the relevant advantages of metabolomics approach and disulfide-bridged substructure which is more frequently imprinted in the marketed peptide drug molecules. Acalitide is biosynthetically unique in the macrotricyclization via two disulfide bridges and a protease (AcaB)-catalyzed lactamization of AcaA, an unprecedented precursor peptide. Such a biosynthetic logic was successfully re-edited for its sample supply renewal to facilitate the identification of the in vitro and in vivo antiparkinsonian efficacy of acalitide which was further confirmed safe and rendered brain-targetable by the liposome encapsulation strategy. Taken together, the work updates the mining strategy and biosynthetic complexity of RiPPs to unravel an antiparkinsonian drug candidate valuable for combating Parkinson's disease that is globally prevailing in an alarming manner.
ABSTRACT
An isolate of rare actinobacteria strain Amycolatopsis sp. HCa1 obtained from the gut of grasshopper produced seven different metabolites in vitro. The metabolites isolated from its mycelia cakes were characterized by NMR and MS analyses. Actinotetraose hexatiglate (or tigloside; 1) with nonreducing glucotetraose skeleton was isolated as a major constituent; three new tetrasaccharide derivatives actinotetraoses I-K (2-4, resp.) and three known actinotetraoses A-C (5-7, resp.) were also isolated.
Subject(s)
Actinobacteria/chemistry , Grasshoppers/microbiology , Oligosaccharides/chemistry , Actinobacteria/isolation & purification , Animals , Crotonates/chemistry , Crotonates/isolation & purification , Magnetic Resonance Spectroscopy , Mass Spectrometry , Oligosaccharides/isolation & purificationABSTRACT
A new alkaloid was isolated from the ethyl acetate extract of the culture of a termite-associated Streptomyces koyangensis BY-4. The structure of 1 was elucidated by using MS, NMR, electronic circular dichroism data, and computational approaches. Compound 1 showed weak antimicrobial activities against a panel of test microbes.
Subject(s)
Alkaloids/isolation & purification , Indolizines/isolation & purification , Isoptera/microbiology , Streptomyces/chemistry , Alkaloids/chemistry , Alkaloids/pharmacology , Animals , Aspergillus niger/drug effects , Bacillus subtilis/drug effects , Candida albicans/drug effects , Circular Dichroism , Cryptococcus neoformans/drug effects , Escherichia coli/drug effects , Indolizines/chemistry , Indolizines/pharmacology , Microbial Sensitivity Tests , Micrococcus luteus/drug effects , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Pseudomonas aeruginosa/drug effects , Staphylococcus aureus/drug effects , Streptococcus pyogenes/drug effectsABSTRACT
Chemical study of the ethyl acetate extract of the plant endophytic fungus Cladosporium sp. (strain no. IFB3lp-2) yielded three new polyketides (1-3), together with nine known compounds. All of the structures were elucidated on the basis of spectroscopic methods. The isolated compounds were screened for their cytotoxic, antiviral, and acetyl cholinesterase inhibitory activities. Regretfully, no compounds showed any significant activity in these assays.
Subject(s)
Antiviral Agents/isolation & purification , Cholinesterase Inhibitors/isolation & purification , Cladosporium/chemistry , Polyketides/isolation & purification , Antiviral Agents/chemistry , Antiviral Agents/pharmacology , China , Cholinesterase Inhibitors/chemistry , Cholinesterase Inhibitors/pharmacology , Drug Screening Assays, Antitumor , Female , HCT116 Cells , Hep G2 Cells , Humans , Microbial Sensitivity Tests , Molecular Structure , Plant Leaves/microbiology , Polyketides/chemistry , Polyketides/pharmacology , Rhizophoraceae/microbiologyABSTRACT
A type II polyketide synthase biosynthetic gene cluster (nap) was identified in Streptomyces eurocidicus CGMCC 4.1086 via genome mining. The heterologous expression of the cryptic nap gene cluster in Streptomyces albus J1074 generated dimerized aromatic polyketide naphthocyclinones (1-3), whose structures were determined via extensive analysis using nuclear magnetic resonance and high-resolution electrospray ionization mass spectroscopy. The biological pathway of naphthocyclinone synthesis was revealed via in vivo gene deletion, in vitro biochemical reactions, and comparative genomics. Remarkably, 3 played a crucial role in inhibiting Phytophthora capsici and Phytophthora sojae, with EC50 values of 6.1 and 20.2 µg/mL, respectively. Furthermore, 3 exhibited a potent protective effect against P. capsici and P. sojae in greenhouse tests.
Subject(s)
Anti-Infective Agents , Streptomyces , Naphthalenes/metabolism , Anti-Infective Agents/pharmacology , Anti-Infective Agents/metabolism , Streptomyces/genetics , Streptomyces/metabolism , Multigene FamilyABSTRACT
Drimane-type sesquiterpenoids are widely distributed in fungi. From the ethyl acetate extract of the earwig-derived Aspergillus sp. NF2396, seven new drimane-type sesquiterpenoids, named drimanenoids A-G (1-7), were isolated. Their structures were elucidated by diverse spectroscopic analysis including high-resolution ESI-MS, one- and two-dimensional NMR spectroscopy. Drimanenoids A-F (1-6) are new members of drimane-type sesquiterpenoid esterified with unsaturated fatty acid side chain at C-6. Drimanenoids C (3), D (4) and F (6) showed antibacterial activity against five types of bacteria with different inhibition diameters. Drimanenoid D (4) exhibited moderate cytotoxicity against human myelogenous leukemia cell line K562 with an IC50 value of 12.88 ± 0.11 µmol·L-1.