ABSTRACT
[This retracts the article DOI: 10.1007/s12088-011-0068-7.].
ABSTRACT
Community level physiological profiles (CLPPs) have been rarely applied to mushroom compost ecosystem, probably for the lack of standardized methodology. Recently, however CLPPs have been employed as a tool to investigate the degree of maturity of compost (Mondini and Insam, 2005, Compost Science and Utilization, 13(1): 27-33). The potential of CLPPs to detect compost maturity test is considerably significant in that it provides sensitivity and the simplicity of the assay. The aim of this work was to investigate the maturity of casing that comprised of farm yard manure and spent compost and influence of casing type on the behaviour of bacterial community during the growth cycle of mushroom Agaricus bisporus (Lange) Imbach employing standardized inoculum density and effects of different data interpretation based on the kinetics of colour formation. Casing samples of different age were extracted at a particular dilution and then inoculated in 96 well microtitre plates. Optical density (OD) in well was measured at 590 nm every 24 hours for 5 days. Principal component analysis (PCA) was performed by employing OD values at fixed average well colour development (AWCD). PCA of fresh samples showed that classification and ordination of samples according to their age were significant with fixed AWCD.
ABSTRACT
Plant growth-promoting rhizobacteria (PGPR) that produce antifungal metabolites are potential threats for the arbuscular mycorrhizal (AM) fungi known for their beneficial symbiosis with plants that is crucially important for low-input sustainable agriculture. To address this issue, we used a compartmented container system where test plants, Vigna radiata, could only reach a separate nutrient-rich compartment indirectly via the hyphae of AM fungi associated with their roots. In this system, where plants depended on nutrient uptake via AM symbiosis, we explored the impact of various PGPR. Plants were inoculated with or without a consortium of four species of AM fungi (Glomus coronatum, Glomus etunicatum, Glomus constrictum, and Glomus intraradices), and one or more of the following PGPR strains: phenazine producing (P(+)) and phenazine-less mutant (P(-)), diacetylphloroglucinol (DAPG) producing (G(+)) and DAPG-less mutant (G(-)) strains of Pseudomonas fluorescens, and an unknown antifungal metabolite-producing Alcaligenes faecalis strain, SLHRE425 (D). PGPR exerted only a small if any effect on the performance of AM symbiosis. G(+) enhanced AM root colonization and had positive effects on shoot growth and nitrogen content when added alone, but not in combination with P(+). D negatively influenced AM root colonization, but did not affect nutrient acquisition. Principal component analysis of all treatments indicated correlation between root weight, shoot weight, and nutrient uptake by AM fungus. The results indicate that antifungal metabolites producing PGPR do not necessarily interfere with AM symbiosis and may even promote it thus carefully chosen combinations of such bioinoculants could lead to better plant growth.
Subject(s)
Antibiosis , Bacterial Physiological Phenomena , Fabaceae/growth & development , Fabaceae/microbiology , Mycorrhizae/drug effects , Mycorrhizae/growth & development , Alcaligenes faecalis/physiology , Antifungal Agents/metabolism , Antifungal Agents/pharmacology , Biomass , Colony Count, Microbial , Phenazines/metabolism , Phenazines/pharmacology , Phloroglucinol/metabolism , Phloroglucinol/pharmacology , Plant Roots/growth & development , Plant Roots/microbiology , Plant Shoots/growth & development , Pseudomonas fluorescens/physiologyABSTRACT
Microorganisms and plants sustain themselves under iron-deprived conditions by releasing siderophores. Among others, fluorescent pseudomonads are known to exert extensive biocontrol action against soil and root borne phytopathogens through release of antimicrobials and siderophores. In this study, production and regulation of siderophores by fluorescent Pseudomonas strain GRP3A was studied. Among various media tested, standard succinate medium (SSM) promoted maximum siderophore production of 56.59 mg l(-1). There were low levels of siderophore in complex media like King's B medium, trypticase soya medium and nutrient medium (41.27, 29.86 and 27.63 mg l(-1)), respectively. In defferrated SSM, siderophore level was quantified to be 68.74 mg l(-1). Supplementation with iron (FeCl3) resulted in decreased siderophore levels depending on concentration. Siderophore production was promoted by Zn2+ (78.94 mg l(-1)), Cu2+ (68.80 mg l(-1)) whereas Co2+ (57.33 mg l(-1)) and Fe3+ reduced siderophore production (37.44 mg l(-1) as compared to control (55.97 mg l(-1)). Strain GRP3A showed plant growth promotion under iron limited conditions.
Subject(s)
Fabaceae/microbiology , Iron/pharmacology , Pseudomonas fluorescens/growth & development , Carbon/pharmacology , Chlorides , Culture Media/chemistry , Culture Media/pharmacology , Fabaceae/drug effects , Fabaceae/growth & development , Ferric Compounds/pharmacology , Fluorescence , Iron/metabolism , Iron Deficiencies , Pseudomonas fluorescens/drug effects , Pseudomonas fluorescens/metabolism , Siderophores/biosynthesis , Succinic Acid/pharmacology , Trace Elements/pharmacologyABSTRACT
A combination of cultivation-based methods with a molecular biological approach was employed to investigate whether bacteria with identical 16S rRNA gene sequences can represent distinct eco- and genotypes. A set of eight bacterial strains wherein three were Pseudomonas putida and rest were Acinetobacter calcoaceticus, were isolated from casing soils community by conventional plating. These strains had identical 16S rRNA gene sequences and represented the dominant phylotype in the plateable fraction. Each strain utilized a specific combination of 154 carbon substrates, and the niche overlap indices were low, suggesting that each strain occupied a different ecological niche. Our results have implications for assessment of the diversity and biogeography of bacteria and increase the perception of natural diversity beyond the level of 16S rRNA gene sequences. It is worthwhile approach to explore prokaryotic diversity in different ecological niches.
ABSTRACT
Biological control of soil-borne pathogens comprises the decrease of inoculum or of the disease producing activity of a pathogen through one or more mechanisms. Interest in biological control of soil-borne plant pathogens has increased considerably in the last few decades, because it may provide control of diseases that cannot or only partly be managed by other control strategies. Recent advances in microbial and molecular techniques have significantly contributed to new insights in underlying mechanisms by which introduced bacteria function. Colonization of plant roots is an essential step for both soil-borne pathogenic and beneficial rhizobacteria. Colonization patterns showed that rhizobacteria act as biocontrol agents or as growth-promoting bacteria form microcolonies or biofilms at preferred sites of root exudation. Such microcolonies are sites for bacteria to communicate with each other (quorum sensing) and to act in a coordinated manner. Elicitation of induced systemic resistance (ISR) by plant-associated bacteria was initially demonstrated using Pseudomonas spp. and other Gram-negative bacteria. Several strains of the species Bacillus amyloliquefaciens, B. subtilis, B. pasteurii, B. cereus, B. pumilus, B. mycoides, and B. sphaericus elicit significant reductions in the incidence or severity of various diseases on a diversity of hosts. Elicitation of ISR by these strains has been demonstrated in greenhouse or field trials on tomato, bell pepper, muskmelon, watermelon, sugar beet, tobacco, Arabidopsis sp., cucumber, loblolly pine, and two tropical crops (long cayenne pepper and green kuang futsoi). Protection resulting from ISR elicited by Bacillus spp. has been reported against leaf-spotting fungal and bacterial pathogens, systemic viruses, a crown-rotting fungal pathogen, root-knot nematodes, and a stem-blight fungal pathogen as well as damping-off, blue mold, and late blight diseases. This progress will lead to a more efficient use of these strains which is worthwhile approach to explore in context of biocontrol strategies.
Subject(s)
Bacillus/physiology , Immunity, Innate , Plant Diseases/immunology , Plants/microbiology , Plant Diseases/microbiology , Plant Roots/immunology , Plant Roots/microbiology , Plants/immunologyABSTRACT
The cyclodepsipeptide serratamolide A ( 1) and five closely related compounds together with three new glucosamine derivatives were isolated by bioactivity-guided chromatography from the XAD adsorber resin extract of a Serratia sp. The structures of the compounds were elucidated by 2D NMR and MS analyses. In addition to the known serratamolide A ( 1) with two C 10 alkyl chains, its derivatives always contained one C 10 chain combined with either C 12:1, C 12, C 11, C 9, or C 8 chains. The glucosamine derivatives contained a common core consisting of an N-butyl-alpha-glucopyranosylamide, which was acylated at the C-1 oxygen with valine. The differences between the derivatives arise from the nature of the acyl groups attached to the N-terminus of valine, which were identified as the linear fatty acid moieties C 16:1, C 15, or C 14. Each compound was present in two isomeric forms arising from racemization of the valine moiety. All compounds showed antibiotic activity against Mycobacterium diernhoferi and other rapidly growing mycobacteria.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Depsipeptides/isolation & purification , Depsipeptides/pharmacology , Mycobacterium/drug effects , Anti-Bacterial Agents/chemistry , Depsipeptides/chemistry , Fatty Acids/chemistry , Microbial Sensitivity Tests , Molecular Structure , SerratiaABSTRACT
Based on in vitro screening for PGP and anti-mycelial activity against three zoosporic pathogenic oomycetes, Pythium aphanidermatum 123, P. aphanidermatum 4746, and Phytophthora nicotianae 4747, seven bacterial isolates were selected for field trials on tomato and chile to test for plant growth promotion under natural and artificial disease-infested field sites in both winter and wet seasons. The effectiveness of isolates in the field trials correlated with the in vitro antagonism screening data. Pseudomonas sp. FQP PB-3, FQA PB-3 and GRP(3) showed substantial beneficial effects on plant growth promotion and lowered considerably the incidence of pre- and post-emergence damping-off in both the crops under various disease scenarios. For example, seed bacterization with these bacterial strains reduced pre-emergence-damping off by ca. 60-70% in the two natural sites, with and without histories of fungicide use in the winter season, and to a lesser extent, ca. 20-40%, in the warmer wet (high humidity; 85-92%) season. The suppression efficacy for post-emergence damping-off was less compared to pre-emergence damping-off although still significant (P > 0.05). Our data unambiguously show that screening of a large number of bacterial pool identifies promising isolates that show beneficial effects on all stages of plant growth in natural oomycete-infested regimes.
Subject(s)
Antibiosis , Capsicum/microbiology , Plant Diseases/microbiology , Pseudomonas/metabolism , Soil Microbiology , Solanum lycopersicum/microbiology , Capsicum/drug effects , Capsicum/growth & development , Fungicides, Industrial/pharmacology , India , Solanum lycopersicum/drug effects , Solanum lycopersicum/growth & development , Phytophthora/microbiology , Pythium/microbiologyABSTRACT
Pythium and Phytophthora species are associated with damping-off diseases in vegetable nurseries and reduce seedling stand and yield. In this study, bacterial isolates were selected on the basis of in vitro antagonism potential to inhibit mycelial growth of damping-off pathogens along with plant growth properties for field assessment in wet and winter seasons. We demonstrate efficacy of bacterial isolates to protect chile and tomato plants under natural vegetable nursery and artificially created pathogen-infested (Pythium and Phytophthora spp.) nursery conditions. After 21 days of sowing, chile and tomato plants were harvested and analysed for peroxidase and phenylalanine ammonia-lyase activities. Pseudomonas sp. strains FQP PB-3, FQA PB-3 and GRP(3 )were most effective in increasing shoot length (P > 0.05%) in both artificial and natural field sites. For example, Pseudomonas sp. FQA PB-3 treatment increased shoot length by 40% in the artificial Pythium 4746 infested nursery site in chile plants in the wet season. The bacterial treatments significantly increased the activity of peroxidase and phenylalanine ammonia-lyase in chile and tomato plant tissues, which are well known as indicators of an active lignification process. Thus, we conclude that treatment with potential bacterial plant growth promoting agents help plants against pathogen invasion by modulating plant peroxidase and phenylalanine ammonia-lyase activities.
Subject(s)
Antibiosis , Capsicum , Gene Expression Regulation, Enzymologic , Pest Control, Biological , Phytophthora/growth & development , Pseudomonas/classification , Pythium/growth & development , Solanum lycopersicum , Capsicum/enzymology , Capsicum/growth & development , Capsicum/microbiology , Gene Expression Regulation, Plant , Solanum lycopersicum/enzymology , Solanum lycopersicum/growth & development , Solanum lycopersicum/microbiology , Plant Diseases/microbiology , Plant Roots/microbiology , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Pseudomonas/genetics , Pseudomonas/growth & development , Pseudomonas/isolation & purification , Soil MicrobiologyABSTRACT
A detailed screening of bacterial isolates from the Central Himalayan region for plant growth promotion and antimycelial activity against Pythium and Phytophthora strains afforded seven isolates, of which three were particularly effective against the incidence of damping-off in field trials on chilli and tomato. In this investigation an initial spectroscopic survey of the methanolic extracts of the seven bacterial isolates showed complex mixtures except for Pseudomonas sp. GRP3, one of the most promising isolates on the basis of field studies. Strain GRP3 was selected for structural characterization of its secondary metabolites, particularly glycolipids. The extracellular secondary metabolites were enriched by Amberlite XAD-16 adsorber resin followed by separation and structural analysis using TLC, LC-MS, MS-MS, and NMR spectroscopy. Acquired data show the presence of a number of mono- and dirhamnolipids and include rhamnose (Rha)-C8-C10, Rha-C10-C8, Rha-C10-C10, Rha-C10-C12:1, Rha-C10-C12, Rha-Rha-C8-C10, Rha-Rha-C10-C10, Rha-Rha-C10-C10:1, Rha-Rha-C10-C12, Rha-Rha-C10-C12:1, Rha-Rha-C12-C12:1, and Rha-Rha-C12-C12 in strain GRP3. Since rhamnolipids are involved in the lysis of the plasma membrane of zoospores of fungi, application of such rhamnolipid-producing rhizobacteria could facilitate control of damping-off plant pathogens.
Subject(s)
Capsicum/drug effects , Glycolipids/isolation & purification , Glycolipids/pharmacology , Plant Diseases , Pseudomonas/chemistry , Solanum lycopersicum/drug effects , Glycolipids/chemistry , Glycolipids/classification , Molecular Structure , Pseudomonas/metabolism , Spectrometry, Mass, Electrospray IonizationABSTRACT
Biological control is an accepted important component of current plant disease management strategies. Introduction of bacterized seeds carrying bacterial isolates with proven growth-promotion capabilities and antagonistic characteristics offer a valid alternative to chemical protectants. Root colonization of disease-susceptible (PS 1024) and moderately resistant (PS1042) varieties of soyabean (Glycine Max L) by fluorescent pseudomonad (FLPs) strains GRP3, PEn-4, PRS1, and WRS-24 was studied in relation to natural occurrence of anthracnose caused by Colletotrichum dematium (Pers Ex Fr.) Grove. Rhizoplane population of FLPs was maintained at a critical level (5.3 cfu) up to 30 days of plant growth, followed by a steep decline. Indigenous FLPs population, however, remained nearly unchanged (3.0 to 2.4 log g(-1) root) between 30 days and 75 days of plant growth. The relative FLPs population in rhizosphere was lower than that in rhizoplane. Although intervarietal difference was observed, the root/shoot length remained unaffected. Compared to nonbacterized control, dry root weight was improved by FLPs treatment. Severity of foliar anthracnose was reduced significantly after FLPs treatment in the variety PS 1042. Because the point of FLPs treatment (seed bacterization) was away from the site of disease appearance (leaf), operation of induced systemic resistance in strains PEn-4 and GRP3 appears imminent.
Subject(s)
Colletotrichum/growth & development , Glycine max/microbiology , Pest Control, Biological , Pseudomonas/physiology , Plant Diseases/microbiology , Plant Roots/microbiology , Seeds/microbiology , Glycine max/growth & developmentABSTRACT
The rhizosphere or the zone of influence around roots harbors a multitude of microorganisms that are affected by both abiotic and biotic stresses. Among these are the dominant rhizobacteria that prefer living in close vicinity to the root or on its surface and play a crucial role in soil health and plant growth. Both free-living and symbiotic bacteria are involved in such specific ecological niches and help in plant matter degradation, nutrient mobilization and biocontrol of plant disease. While the rhizosphere as a domain of fierce microbial activity has been studied for over a century, the availability of modern tools in microbial ecology has now permitted the study of microbial communities associated with plant growth and development, in situ localization of important forms, as well as the monitoring of introduced bacteria as they spread in the soil and root environment. This interest is linked to environmental concerns for reduced use of chemicals for disease control as well as an appreciation for utilization of biologicals and organics in agriculture. Indian researchers have studied the diversity of rhizobacteria in a variety of plants, cereals, legumes and others along with assessment of their functionality based on the release of enzymes (soil dehydrogenase, phosphatase, nitrogenase, etc.), metabolites (siderophores, antifungals, HCN, etc.), growth promoters (IAA, ethylene) and as inducers of systemic disease resistance (ISR). Based on such primary screening protocols, effective rhizobacteria have been field tested with success stories from various agroecological zones of the country, as reflected in the control of root- and soil-borne diseases, improved soil health and increased crop yields. Several commercial formulations, mostly based on dry powder (charcoal, lignite, farmyard manure, etc.) have been prepared and field tested, however, problems of appropriate shelf-life and cell viability are still to be solved. Also, inherent in such low cost technologies are the problems of variability in field performance and successful establishment of introduced inoculants in the root zone. In addition, most products available in the market are not properly monitored for quality before they reach the farmer. As a consequence, the acceptance of rhizobacterial formulations in the country is limited. However, several laboratories have now developed protocols for the rapid characterization of effective isolates based on molecular fingerprinting and other similar tools. Also, the use of molecular markers (gus, lux, gfp, etc.) makes it easy to monitor introduced inoculants in situ in soil and rhizosphere environments. The government initiative in integrated nutrient management and pest management systems has provided additional incentives to relate rhizobacterial science to other ongoing activities so that the benefit of this research leads to technologies that are environmentally and socially acceptable.