Differences in protein fractions of avian plasma among three commercial electrophoresis systems.

Previous studies have defined the presence of 6 protein fractions in plasma from many psittaciform species. Additionally, extensive reference intervals have been published for many of these species with the Beckman Paragon electrophoresis system, which had been commonly used in clinical laboratories to analyze the protein fractions of avian plasma. In mid-2009, Beckman discontinued the Paragon product line, leaving 2 primary alternative systems: Helena and Sebia. To compare electrophoresis results from the 3 commercial protein electrophoresis systems, specimens from 40 African grey parrots (Psittacus erithacus) were analyzed with the electrophoresis systems from Beckman, Helena, and Sebia. Marked differences in fraction migration were found between the Beckman/Helena and Sebia systems, which manifested as a large decrease in prealbumin and an increase in alpha1 globulins in the latter system. Both proportional and constant errors were observed among the fraction quantitation data of both the Helena and Sebia systems compared with the Beckman system. Based on Bland-Altman plot data and imprecision studies, the Helena system appears more similar with the Beckman system, although neither the Helena nor the Sebia systems are identical to the Beckman system. Because of the differences in electrophoresis methods, clinicians should be careful to consistently use particular clinical laboratories. For best application, reference intervals should be established based on both species and electrophoresis system.

A dog model to investigate the relationship between obstructive sleep apnoea and blood pressure regulation.

Patients exhibiting obstructive sleep apnoea (OSA) do not display a normal circadian pattern of blood pressure. It is not clear whether this disruption of the circadian blood pressure pattern is a result of the intermittent airway obstruction during sleep or is the result of confounding factors, such as obesity and age, which are common in OSA and may independently affect blood pressure. To determine if a cause and effect relationship exists between repetitive airway obstruction during sleep and blood pressure regulation a chronically instrumented canine model of OSA has been developed. This canine model has been shown to reproduce the characteristic apnoea and hypersomnolence of human OSA. Furthermore, in this model a 12-h nocturnal period of repetitive airway obstruction during sleep caused an increase in baseline blood pressure of more than 10 mmHg that was sustained for at least two hours following the restoration of normal airway patency. These results imply that there is a cause and effect relationship between intermittent airway obstruction during sleep and elevated blood pressure.

Effect of hypertension on upper airway function and sleep apnoea.

Current evidence suggests that elevations in blood pressure during obstructive apnoeic episodes increase pharyngeal collapsibility and the severity of obstructive sleep apnoea.

The socially weighted encoding of spoken words: a dual-route approach to speech perception.

Spoken words are highly variable. A single word may never be uttered the same way twice. As listeners, we regularly encounter speakers of different ages, genders, and accents, increasing the amount of variation we face. How listeners understand spoken words as quickly and adeptly as they do despite this variation remains an issue central to linguistic theory. We propose that learned acoustic patterns are mapped simultaneously to linguistic representations and to social representations. In doing so, we illuminate a paradox that results in the literature from, we argue, the focus on representations and the peripheral treatment of word-level phonetic variation. We consider phonetic variation more fully and highlight a growing body of work that is problematic for current theory: words with different pronunciation variants are recognized equally well in immediate processing tasks, while an atypical, infrequent, but socially idealized form is remembered better in the long-term. We suggest that the perception of spoken words is socially weighted, resulting in sparse, but high-resolution clusters of socially idealized episodes that are robust in immediate processing and are more strongly encoded, predicting memory inequality. Our proposal includes a dual-route approach to speech perception in which listeners map acoustic patterns in speech to linguistic and social representations in tandem. This approach makes novel predictions about the extraction of information from the speech signal, and provides a framework with which we can ask new questions. We propose that language comprehension, broadly, results from the integration of both linguistic and social information.

VARICOSE, a WD-domain protein, is required for leaf blade development.

To gain insight into the processes controlling leaf development, we characterized an Arabidopsis mutant, varicose (vcs), with leaf and shoot apical meristem defects. The vcs phenotype is temperature dependent; low temperature growth largely suppressed defects, whereas high growth temperatures resulted in severe leaf and meristem defects. VCS encodes a putative WD-domain containing protein, suggesting a function involving protein-protein interactions. Temperature shift experiments indicated that VCS is required throughout leaf development, but normal secondary vein patterning required low temperature early in leaf development. The low-temperature vcs phenotype is enhanced in axr1-3 vcs double mutants and in vcs mutants grown in the presence of polar auxin transport inhibitors, however, vcs has apparently normal auxin responses. Taken together, these observations suggest a role for VCS in leaf blade formation.