ABSTRACT
This work first showed that very high amounts of phycocyanins, such as 11.3 mg/mL C-phycocyanin (C-PC), 3.1 mg/mL allophycocyanin (APC), and 0.8 mg/mL phycoerythrin (PE), can be obtained using an ultrasonic extraction process (UE) with a 60 kHz frequency and 3 h of process time at 25 °C, without any other pretreatments. These yields were higher than those from most conventional water extractions at 4 °C for 24 h (Control condition) or at 25 °C for 24 h (WE), namely, 9.8 and 5.7 mg/mL C-PC, 2.3 and 1.2 mg/mL APC, and 0.7 and 0.3 mg/mL PE, respectively. These yields were also shown to be even higher than yields from other reported data. Structural changes in C-PC in the extracts were also found for the first time, according to extraction conditions, showing that the total concentration of C-PC and of the α-subunit of C-PC in the UE were much higher than in the WE, with little difference in the amount of ß-subunit of C-PC in the UE or WE. It was also shown that the structural changes in C-PC in the WE decreased both antioxidant and anti-inflammation activities-29.83% vs. 32.09% of α,α-diphenyl-ß-picrylhydrazyl (DPPH) scavenging activity and 8.21 vs. 7.25 µM of NO production for the WE and UE, respectively-while the UE, with similar patterns to standard C-PC, showed very high biological effects, which may suggest that the biologically active part is the α-subunit of C-PC, not the ß-subunit.
Subject(s)
Free Radical Scavengers/chemistry , Phycocyanin/chemistry , Spirulina/radiation effects , Ultrasonic Waves , Chemical Fractionation/methods , Free Radical Scavengers/metabolism , Phycocyanin/metabolism , Spirulina/chemistry , Spirulina/metabolismABSTRACT
BACKGROUND: Glutamate (an endogenous excitatory neurotransmitter) at high concentrations contributes to the development of neurodegenerative diseases. Aronia melanocarpa (A. melanocarpa) berries contain anthocyanins and have high antioxidant activities. In this study, we evaluated whether A. melanocarpa berries could protect neuronal cells against glutamate-induced oxidative stress. METHOD: A. melanocarpa berries exerted a protective effect against cytotoxicity in HT22 mouse hippocampal cells by MTT assay. We evaluated oxidative stress parameters including ROS level, intracellular Ca2+ level, glutathione level and antioxidant enzyme activity in HT22 cells to elucidate the mechanism of its neuroprotective effect. RESULTS: A. melanocarpa berries decreased glutamate-induced death of HT22 cells. In addition, A. melanocarpa berries reduced ROS and intracellular Ca2+ levels. Glutathione level, antioxidant enzymes, glutathione reductase and glutathione peroxide activities and mitochondrial membrane potential were also increased in HT22 cells. CONCLUSION: These results suggested that A. melanocarpa berries protected HT22 cells by exerting an antioxidant effect.
Subject(s)
Glutamic Acid/adverse effects , Neurodegenerative Diseases/metabolism , Neurons/drug effects , Neuroprotective Agents/pharmacology , Oxidative Stress/drug effects , Photinia/chemistry , Plant Extracts/pharmacology , Animals , Cell Line, Tumor , Fruit/chemistry , Glutathione/metabolism , Humans , Mice , Neurodegenerative Diseases/drug therapy , Neurons/metabolism , Reactive Oxygen Species/metabolismABSTRACT
Spirulina maxima, a microalga containing high levels of protein and many polyphenols, including chlorophyll a and C-phycocyanin, has antioxidant and anti-inflammatory therapeutic effects. However, the mechanisms where by Spirulina maxima ameliorates cognitive disorders induced by amyloid-ß 1-42 (Aß1-42) are not fully understood. In this study, we investigated whether a 70% ethanol extract of Spirulina maxima (SM70EE) ameliorated cognitive impairments induced by an intracerebroventricular injection of Aß1-42 in mice. SM70EE increased the step-through latency time in the passive avoidance test and decreased the escape latency time in the Morris water maze test in Aß1-42-injected mice. SM70EE reduced hippocampal Aß1-42 levels and inhibited amyloid precursor protein processing-associated factors in Aß1-42-injected mice. Additionally, acetylcholinesterase activity was suppressed by SM70EE in Aß1-42-injected mice. Hippocampal glutathione levels were examined to determine the effects of SM70EE on oxidative stress in Aß1-42-injected mice. SM70EE increased the levels of glutathione and its associated factors that were reduced in Aß1-42-injected mice. SM70EE also promoted activation of the brain-derived neurotrophic factor/phosphatidylinositol-3 kinase/serine/threonine protein kinase signaling pathway and inhibited glycogen synthase kinase-3ß phosphorylation. These findings suggested that SM70EE ameliorated Aß1-42-induced cognitive impairments by inhibiting the increased phosphorylation of glycogen synthase kinase-3ß caused by intracerebroventricular injection of Aß1-42 in mice.
Subject(s)
Glycogen Synthase Kinase 3 beta/metabolism , Maze Learning , Memory Disorders/drug therapy , Plant Extracts/therapeutic use , Spirulina/chemistry , Acetylcholinesterase/metabolism , Amyloid beta-Peptides/administration & dosage , Amyloid beta-Peptides/toxicity , Animals , Glutathione/metabolism , Hippocampus/drug effects , Hippocampus/metabolism , Injections, Intraventricular , Male , Memory Disorders/etiology , Mice , Mice, Inbred ICR , Peptide Fragments/administration & dosage , Peptide Fragments/toxicity , Phosphorylation , Plant Extracts/pharmacology , Protein Processing, Post-TranslationalABSTRACT
Spirulina maxima is a microalgae which contains flavonoids and other polyphenols. Although Spirulina maxima 70% ethanol extract (SM70EE) has diverse beneficial effects, its effects on neurotoxicity have not been fully understood. In this study, we investigated the neuroprotective effects of SM70EE against trimethyltin (TMT)-induced neurotoxicity in HT-22 cells. SM70EE inhibited the cleavage of poly-ADP ribose polymerase (PARP). Besides, ROS production was decreased by down-regulating oxidative stress-associated enzymes. SM70EE increased the factors of brain-derived neurotrophic factor (BDNF)/cyclic AMPresponsive elementbinding protein (CREB) signalling pathways. Additionally, acetylcholinesterase (AChE) was suppressed by SM70EE. Furthermore, we investigated whether SM70EE prevents cognitive deficits against scopolamine-induced neurotoxicity in mice by applying behavioral tests. SM70EE increased step-through latency time and decreased the escape latency time. Therefore, our data suggest that SM70EE may prevent TMT neurotoxicity through promoting activation of BDNF/CREB neuroprotective signaling pathways in neuronal cells. In vivo study, SM70EE would prevent cognitive deficits against scopolamine-induced neurotoxicity in mice.
Subject(s)
Cell Extracts/chemistry , Neurons/drug effects , Neurotoxicity Syndromes/drug therapy , Spirulina/chemistry , Animals , Brain-Derived Neurotrophic Factor/genetics , CREB-Binding Protein/genetics , Cell Extracts/pharmacology , Cell Line , Flavonoids/chemistry , Flavonoids/pharmacology , Gene Expression Regulation/drug effects , Humans , Mice , Neurons/pathology , Neuroprotective Agents/pharmacology , Neurotoxicity Syndromes/pathology , Poly(ADP-ribose) Polymerases/genetics , Polyphenols/chemistry , Polyphenols/pharmacology , Signal Transduction/drug effectsABSTRACT
This study describes a new effort toward understanding the interaction mechanisms between antibiotic-resistant Salmonella Typhimurium and phages. The antibiotic susceptibility, ß-lactamase activity, bacterial motility, gene expression, and lytic activity were evaluated in ciprofloxacin-induced antibiotic-sensitive Salmonella Typhimurium (ASST(CIP)) and ciprofloxacin-induced antibiotic-resistant S. Typhimurium (ARST(CIP)), which were compared to the wild-type strains (ASST(WT) and ARST(WT)). The MIC values of ampicillin, norfloxacin, chloramphenicol, and tetracycline were significantly increased to > 512, 16, 16, and 256 µg/ml, respectively, in the ARST(CIP). The lowest and highest extracellular lactamase activities were observed in ASST(WT) (6.85 µmol/min/ml) and ARST(CIP) (48.83 µmol/min/ml), respectively. The acrA, lpfE, and hilA genes were significantly upregulated by more than tenfold in both ASST(CIP) and ARST(CIP). The induction of multiple antibiotic resistance resulted from the increased efflux pump activity (AcrAB-TolC). The highest phage adsorption rates were more than 95 % for ASST(WT), ASST(CIP), and ARST(WT), while the lowest adsorption rate was 52 % for ARST(CIP) at 15 min of infection. The least lytic activity of phage was 20 % against the ARST(CIP), followed by ASST(CIP) (30 %). The adsorption rate of phage against ARST(CIP) was 52 % at 15 min of infection, which resulted in the decrease in lytic activity (12 %). Understanding the interaction of phage and bacteria is essential for the practical application of phage to control and detect antibiotic-resistant bacteria. The results provide useful information for understanding the binding specificity of phages for multiple antibiotic-resistant pathogens.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteriophages/genetics , Ciprofloxacin/pharmacology , Drug Resistance, Multiple, Bacterial/genetics , Salmonella typhimurium/drug effects , Salmonella typhimurium/genetics , Ampicillin/pharmacology , Bacterial Proteins/biosynthesis , Bacterial Proteins/genetics , Binding Sites , Chloramphenicol/pharmacology , Membrane Transport Proteins/biosynthesis , Membrane Transport Proteins/genetics , Microbial Sensitivity Tests , Norfloxacin/pharmacology , Salmonella typhimurium/virology , Tetracycline/pharmacology , Trans-Activators/biosynthesis , Trans-Activators/genetics , beta-Lactamases/geneticsABSTRACT
BACKGROUND: The effect of pressure-assisted thermal processing (PATP) on the inactivation of Geobacillus stearothermophilus spores was determined in deionized water, cooked ground beef, egg patty mince, whole milk and mashed potatoes at 105 °C under 500 and 700 MPa. RESULTS: The numbers of G. stearothermophilus spores in deionized water and milk were reduced by more than 6 log CFU mL(-1) at 700 MPa and 105 °C, whereas those in cooked beef were reduced by 4.27 log CFU g(-1). The inactivation patterns of G. stearothermophilus spores in all food matrices followed nonlinear behavior, showing that Weibull model fitted well to the inactivation curves of G. stearothermophilus spores in low-acid foods. CONCLUSION: The complex food matrices caused a protective effect on the inactivation of G. stearothermophilus spores during PATP. The results provide useful information in inactivation kinetics of bacterial spores for validating PATP-processed low-acid foods.
Subject(s)
Food Preservation/methods , Geobacillus stearothermophilus , Spores, Bacterial , Animals , Cattle , Eggs/microbiology , Food Handling/methods , Hot Temperature , Meat/microbiology , Milk/microbiology , Pressure , Solanum tuberosum/microbiology , Spores, Bacterial/physiology , Water MicrobiologyABSTRACT
In this study, we sought to increase the anti-inflammatory activity of Scutellaria baicalensis extracts using a nanoencapsulation process. The ethanol extract of Scutellaria baicalensis was encapsulated with lecithin and two other extracts as follows: aqueous extraction at 100 °C for 24 h (AE), 70% ethanol extracts at 80°C for 24 h (EE), which were also compared as controls. The ethanol extract of S. baicalensis with lecithin was estimated to be 94.3 nm while the encapsulation efficiency of the nanoparticles was measured as 61.4% higher than other encapsulation processes. Antioxidant activity was also observed as 60% inhibition of DPPH radical scavenging activity, and nitric oxide production by RAW264.7 cells was also reduced by 5.1 µM after the addition of 0.5 mg/mL nanoparticles. Only 743.7 pg/mL of PGE2 was produced by RAW 264.7 macrophages after the addition of 0.5 mg/mL of nanoparticles, as compared to 1105.6 pg/mL and 962.3 pg/mL of PGE2 production after the addition of 1.0 mg/mL of aqueous and ethanol extracts, respectively. This is the first report, to our knowledge, of real-time penetration of nanoparticles into human fibroblasts using a confocal scanning microscope.
Subject(s)
Anti-Inflammatory Agents/administration & dosage , Anti-Inflammatory Agents/pharmacology , Nanocapsules/therapeutic use , Plant Extracts/administration & dosage , Plant Extracts/pharmacology , Animals , Antioxidants/pharmacology , Biphenyl Compounds/antagonists & inhibitors , Cell Culture Techniques , Dinoprostone/biosynthesis , Dose-Response Relationship, Drug , Fibroblasts , Humans , Lecithins/chemistry , Mice , Microscopy, Confocal , Nanocapsules/administration & dosage , Nanocapsules/chemistry , Nitric Oxide/biosynthesis , Picrates/antagonists & inhibitors , Rats , Scutellaria baicalensis , Toxicity TestsABSTRACT
In this study, the effect of Codonopsis lanceolata fermented by lactic acid on controlling gene expression levels related to obesity was observed in an oligonucleotide chip microarray. Among 8170 genes, 393 genes were up regulated and 760 genes were down regulated in feeding the fermented C. lanceolata (FCL). Another 374 genes were up regulated and 527 genes down regulated without feeding the sample. The genes were not affected by the FCL sample. It was interesting that among those genes, Chytochrome P450, Dmbt1, LOC76487, and thyroid hormones, etc., were mostly up or down regulated. These genes are more related to lipid synthesis. We could conclude that the FCL possibly controlled the gene expression levels related to lipid synthesis, which resulted in reducing obesity. However, more detailed protein expression experiments should be carried out.
Subject(s)
Codonopsis/metabolism , Obesity/drug therapy , Obesity/genetics , Plant Extracts/pharmacology , Animals , Calcium-Binding Proteins , Cytochrome P-450 Enzyme System/biosynthesis , DNA-Binding Proteins , Fermentation , Gene Expression Profiling , Gene Expression Regulation , Lactic Acid/chemistry , Lipids/biosynthesis , Liver/enzymology , Liver/metabolism , Male , Mice , Mice, Inbred ICR , Mucins/biosynthesis , Oligonucleotide Array Sequence Analysis , Plants, Medicinal/metabolism , Thyroid Hormones/biosynthesis , Tumor Suppressor ProteinsABSTRACT
In this study, the immuno-modulatory and anticancer activities of marine algae, Spirulina maxima grown in deep-sea water (DSW), were investigated. It was found that the extract of S. maxima, cultured in DSW, effectively suppressed the expression of Bcl2 in A549 cells as well as inhibiting various human cancer cells with concentration dependency, which possibly implies that the extracts may play more important roles in controlling cancer cell growth. The secretion of cytokines IL-6 and TNF-α from human B cells was also greatly increased, compared to those of the extract grown in conventional sea-water. The growth of Human Natural Killer (NK) cells in the presence of the extracts from DSW was significantly higher (12.2 × 104 viable cells/mL) when compared to the control (1.1 × 104 viable cells/mL). Based on HPLC analysis, the increase in the biological activities of the extracts from DSW was caused by considerably high amounts of ß-carotene and ascorbic acid because the DSW contained high concentrations and good ratios of several key minerals for biosynthesizing ß-carotene and ascorbic acid, as well as maintaining high cell growth.
Subject(s)
Immunity , Seawater , Spirulina/growth & development , Antineoplastic Agents/pharmacology , B-Lymphocytes/drug effects , B-Lymphocytes/metabolism , Cell Death/drug effects , Cell Line , Cell Proliferation/drug effects , Chromatography, High Pressure Liquid , Humans , Immunity/drug effects , Interleukin-6/metabolism , Killer Cells, Natural/drug effects , Minerals/analysis , Proto-Oncogene Proteins c-bcl-2/metabolism , T-Lymphocytes/drug effects , T-Lymphocytes/metabolism , Tumor Necrosis Factor-alpha/metabolism , WaterABSTRACT
Nanoencapsulation of thiamine dilauryl sulfate (TDS), a vitamin B1 derivative, was proved to effectively inhibit the spore germination of Fusarium oxysporum f. sp. raphani (F. oxysporum), as well as mycelial growth. The average diameter of nanoparticles was measured as 136 nm by being encapsulated with an edible encapsulant, lecithin, whose encapsulation efficiency was about 55% in containing 200 ppm of TDS concentration: the 100 ppm TDS nanoparticle solution showed a mycelial growth inhibition rate of 59%. These results were about similar or even better than the cases of treating 100 ppm of dazomet, a positive antifungal control (64%). Moreover, kinetic analysis of inhibiting spore germination were estimated as 6.6% reduction of spore germination rates after 24 h treatment, which were 3.3% similar to the case of treating 100 ppm of a positive control (dazomet) for the same treatment time. It was also found that TDS itself could work as an antifungal agent by inhibiting both mycelial growth and spore germination, even though its efficacy was lower than those of nanoparticles. Nanoparticles especially played a more efficient role in limiting the spore germination, due to their easy penetration into hard cell membranes and long resident time on the surface of the spore shell walls. In this work, it was first demonstrated that the nanoparticle of TDS not a harmful chemical can control the growth of F. oxysporum by using a lower dosage than commercial herbicides, as well as the inhibiting mechanism of the TDS. However, field trials of the TDS nanoparticles encapsulated with lecithin should be further studied to be effectively used for field applications.
ABSTRACT
A method for stably purifying a functional dye, phycocyanin from Spirulina platensis was developed by a hexane extraction process combined with high pressure. This was necessary because this dye is known to be very unstable during normal extraction processes. The purification yield of this method was estimated as 10.2%, whose value is 3%-5% higher than is the case from another conventional separation method using phosphate buffer. The isolated phycocyanin from this process also showed the highest purity of 0.909 based on absorbance of 2.104 at 280 nm and 1.912 at 620 nm. Two subunits of phycocyanin namely α-phycocyanin (18.4 kDa) and ß-phycocyanin (21.3 kDa) were found to remain from the original mixtures after being extracted, based on SDS-PAGE analysis, clearly demonstrating that this process can stably extract phycocyanin and is not affected by extraction solvent, temperature, etc. The stability of the extracted phycocyanin was also confirmed by comparing its DPPH (α,α-diphenyl-ß-picrylhydrazyl) scavenging activity, showing 83% removal of oxygen free radicals. This activity was about 15% higher than that of commercially available standard phycocyanin, which implies that the combined extraction method can yield relatively intact chromoprotein through absence of degradation. The results were achieved because the low temperature and high pressure extraction effectively disrupted the cell membrane of Spirulina platensis and degraded less the polypeptide subunits of phycocyanin (which is a temperature/pH-sensitive chromoprotein) as well as increasing the extraction yield.
Subject(s)
Bacterial Proteins/isolation & purification , Phycocyanin/isolation & purification , Spirulina/chemistry , Bacterial Proteins/chemistry , Phycocyanin/chemistry , PressureABSTRACT
Marine microalga, Scenedesmus sp., which is known to be suitable for biodiesel production because of its high lipid content, was subjected to the conventional Folch method of lipid extraction combined with high-pressure homogenization pretreatment process at 1200 psi and 35°C. Algal lipid yield was about 24.9% through this process, whereas only 19.8% lipid can be obtained by following a conventional lipid extraction procedure using the solvent, chloroform:methanol (2:1, v/v). Present approach requires 30 min process time and a moderate working temperature of 35°C as compared to the conventional extraction method which usually requires >5 hrs and 65°C temperature. It was found that this combined extraction process followed second-order reaction kinetics, which means most of the cellular lipids were extracted during initial periods of extraction, mostly within 30 min. In contrast, during the conventional extraction process, the cellular lipids were slowly and continuously extracted for >5 hrs by following first-order kinetics. Confocal and scanning electron microscopy revealed altered texture of algal biomass pretreated with high-pressure homogenization. These results clearly demonstrate that the Folch method coupled with high-pressure homogenization pretreatment can easily destruct the rigid cell walls of microalgae and release the intact lipids, with minimized extraction time and temperature, both of which are essential for maintaining good quality of the lipids for biodiesel production.
Subject(s)
Cell Fractionation/methods , Lipid Metabolism/physiology , Lipids/isolation & purification , Liquid-Liquid Extraction/methods , Scenedesmus/chemistry , Scenedesmus/metabolism , Oceans and Seas , PressureABSTRACT
Ethanol production using hemicelluloses has recently become a focus of many researchers. In order to promote D: -xylose fermentation, we cloned the bacterial xylA gene encoding for xylose isomerase with 434 amino acid residues from Agrobacterium tumefaciens, and successfully expressed it in Saccharomyces cerevisiae, a non-xylose assimilating yeast. The recombinant strain S. cerevisiae W303-1A/pAGROXI successfully colonized a minimal medium containing D: -xylose as a sole carbon source and was capable of growth in minimal medium containing 2% xylose via aerobic shake cultivation. Although the recombinant strain assimilates D: -xylose, its ethanol productivity is quite low during fermentation with D: -xylose alone. In order to ascertain the key enzyme in ethanol production from D: -xylose, we checked the expression levels of the gene clusters involved in the xylose assimilating pathway. Among the genes classified into four groups by their expression patterns, the mRNA level of pyruvate decarboxylase (PDC1) was reduced dramatically in xylose media. This reduced expression of PDC1, an enzyme which converts pyruvate to acetaldehyde, may cause low ethanol productivity in xylose medium. Thus, the enhancement of PDC1 gene expression may provide us with a useful tool for the fermentation of ethanol from hemicellulose.
Subject(s)
Aldose-Ketose Isomerases/metabolism , Ethanol/metabolism , Pyruvate Decarboxylase/biosynthesis , Saccharomyces cerevisiae Proteins/biosynthesis , Saccharomyces cerevisiae/enzymology , Xylose/metabolism , Agrobacterium/enzymology , Agrobacterium/genetics , Aldose-Ketose Isomerases/genetics , Cloning, Molecular , Ethanol/isolation & purification , Pyruvate Decarboxylase/isolation & purification , Recombinant Proteins/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae Proteins/isolation & purification , TransfectionABSTRACT
An agar-degrading marine bacterium identified as a novel member of the family Flavobacteriaceae (strain S85) was isolated from seawater in Micronesia. The sequenced strain S85 genome is composed of 3,384,629 bp in a circular chromosome, which includes 2,883 complete open reading frames.
Subject(s)
DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Flavobacteriaceae/genetics , Genome, Bacterial , Agar/metabolism , Chromosomes, Bacterial , Flavobacteriaceae/isolation & purification , Flavobacteriaceae/metabolism , Micronesia , Molecular Sequence Data , Open Reading Frames , Seawater/microbiology , Sequence Analysis, DNAABSTRACT
To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l(-1) h(-1), respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production.
Subject(s)
Candida tropicalis/genetics , Candida tropicalis/metabolism , D-Xylulose Reductase/genetics , D-Xylulose Reductase/metabolism , Xylitol/biosynthesis , Base Sequence , Biotechnology , DNA, Fungal/genetics , Fermentation , Fungal Proteins/genetics , Fungal Proteins/metabolism , Genes, Fungal , Kinetics , Mutagenesis, Site-Directed , Mutant Proteins/genetics , Mutant Proteins/metabolism , Mutation , Protein Engineering , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Xylose/metabolismABSTRACT
The aim of this work was to investigate the immunomodulatory activities of Rubus coreanus Miquel extract-loaded gelatin nanoparticles. The mean size of the produced nanoparticles was 143 ± 18 nm with a bandwidth of 76 nm in the size distribution and a maximum size of ~200 nm, which allows effective nanoparticle uptake by cells. Confocal imaging confirmed this, since the nanoparticles were internalized within 30 min and heterogeneously distributed throughout the cell. Zeta-potential measurements showed that from pH = 5 onwards, the nanoparticles were highly negatively charged, which prevents agglomeration to clusters by electrostatic repulsion. This was confirmed by TEM imaging, which showed a well dispersed colloidal solution. The encapsulation efficiency was nearly 60%, which is higher than for other components encapsulated in gelatin nanoparticles. Measurements of immune modulation in immune cells showed a significant effect by the crude extract, which was only topped by the nanoparticles containing the extract. Proliferation of B-, T- and NK cells was notably enhanced by Rubus coreanus-gelatin nanoparticles and in general ~2-3 times higher than control and on average ~2 times higher than ferulic acid. R. coreanus-gelatin nanoparticles induced cytokine secretion (IL-6 and TNF-α) from B- and T-cells on average at a ~2-3 times higher rate compared with the extract and ferulic acid. In vivo immunomodulatory activity in mice fed with R. coreanus-gelatin nanoparticles at 1 mL/g body weight showed a ~5 times higher antibody production compared to control, a ~1.3 times higher production compared to the extract only, and a ~1.6 times higher production compared to ferulic acid. Overall, our results suggest that gelatin nanoparticles represent an excellent transport vehicle for Rubus coreanus extract and extracts from other plants generally used in traditional Asian medicine. Such nanoparticles ensure a high local concentration that results in enhancement of immune cell activities, including proliferation, cytokine secretion, and antibody production.
Subject(s)
Immunologic Factors/pharmacology , Lymphocytes/drug effects , Nanoparticles , Plant Extracts/pharmacology , Rubus/chemistry , Animals , Cell Proliferation/drug effects , Gelatin/chemistry , Humans , Interleukin-6/genetics , Interleukin-6/metabolism , Lymphocytes/metabolism , Mice , Mice, Inbred ICR , Plant Extracts/chemistry , Tumor Necrosis Factor-alpha/genetics , Tumor Necrosis Factor-alpha/metabolismABSTRACT
This study was designed to characterize the viability and potential virulence of bofilm-forming Salmonella enterica serovar Typhimurium under different pH levels, ranging from 5 to 7. The plate count method and real-time reverse transcription-PCR (RT-PCR) were used to evaluate the survival of S. Typhimurium grown in Trypticase soy broth (TSB) adjusted to pH 5, 6, and 7 (TSB-5, TSB-6, and TSB-7, respectively) at 37°C for 10 days. In TSB-5 and TSB-6, the numbers of viable cells estimated by using the real-time RT-PCR were greater than the culturable counts enumerated by the plate count method. Reflectance micro-Fourier transform infrared (micro-FTIR) spectroscopy was used to evaluate the biochemical changes in biofilm cells. Considerable changes in chemical components were observed in the biofilm cells grown in TSB-5 and TSB-6 when compared to the cells grown in TSB-7. The enterotoxin production and invasive ability of planktonic and biofilm S. Typhimurium cells were inferred by the relative levels of expression of stn and invA. The levels of expression of stn and invA were significantly increased in biofilm S. Typhimurium cells grown in TSB-5 (1.9-fold and 3.2-fold) and TSB-6 (2.1-fold and 22.3-fold) after 10 days of incubation. These results suggest that the biofilm-forming S. Typhimurium under different pH levels might change the virulence production and stress response mechanisms.
Subject(s)
Acids/toxicity , Biofilms/growth & development , Salmonella typhimurium/physiology , Colony Count, Microbial , Culture Media/chemistry , Enterotoxins/biosynthesis , Gene Expression Profiling , Hydrogen-Ion Concentration , Metabolome , Microbial Viability/drug effects , Reverse Transcriptase Polymerase Chain Reaction , Salmonella typhimurium/drug effects , Salmonella typhimurium/growth & development , Salmonella typhimurium/pathogenicity , Spectroscopy, Fourier Transform Infrared , Temperature , Time Factors , VirulenceABSTRACT
Farnesyl diphosphate synthase (FPS) plays an essential role in organ development in plants. However, FPS has not previously been identified as a key regulatory enzyme in triterpene biosynthesis. To elucidate the functions of FPS in triterpene biosynthesis, C. asiatica was transformed with a construct harboring Panax ginseng FPS (PgFPS)-encoding cDNA coupled to the cauliflower mosaic virus 35S promoter. Higher levels of CaDDS (C. asiatica dammarenediol synthase) and CaCYS (C. asiatica cycloartenol synthase) mRNA were detected in all hairy root lines overexpressing when compared with the controls. However, no differences were detected in any expression of the CaSQS (C. asiatica squalene synthase) gene. In particular, the upregulation of CaDDS transcripts suggests that FPS may result in alterations in triterpene biosynthesis capacity. Squalene contents in the T17, T24, and T27 lines were increased to 1.1-, 1.3- and 1.5-fold those in the controls, respectively. The total sterol contents in the T24 line were approximately three times higher than those of the controls. Therefore, these results indicated that FPS performs a regulatory function in phytosterol biosynthesis. To evaluate the contribution of FPS to triterpene biosynthesis, we applied methyl jasmonate as an elicitor of hairy roots expressing PgFPS. The results of HPLC analysis revealed that the content of madecassoside and asiaticoside in the T24 line was transiently increased by 1.15-fold after 14 days of MJ treatment. This result may indicate that FPS performs a role not only in phytosterol regulation, but also in triterpene biosynthesis.
Subject(s)
Centella/genetics , Geranyltranstransferase/metabolism , Panax/enzymology , Phytosterols/biosynthesis , Plant Roots/enzymology , Triterpenes/metabolism , Acetates , Centella/enzymology , Chromatography, High Pressure Liquid , Cyclopentanes , Gene Expression Regulation, Plant , Geranyltranstransferase/genetics , Oxylipins , Panax/genetics , Plant Roots/genetics , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/genetics , RNA, Plant/genetics , Squalene/analysis , Transformation, Genetic , Triterpenes/analysis , Up-RegulationABSTRACT
INTRODUCTION: Behenyltrimethylammonium chloride (BTAC) and stearic acid (SA) could be associated with each other through salt bridges, and the associated BTAC/SA could build bilayer vesicles with the aid of hinokitiol (HKL). METHOD: The vesicles were prepared by a precipitation method and used to enhance the skin permeation of HKL. RESULTS: In case the molar ratio of BTAC/SA/HKL was 1/1/0, no vesicle was observed on transmission electron microscope photos. When the molar ratio of BTAC/SA/HKL was 1/1/0.4, vesicle was observed together with some agglomerates. When the content of HKL increased to the ratios of 1/1/0.8 and 1/1/1.2, vesicles were exclusively observed. In vitro fluxes for 18 hours through hairless mouse skin of HKL dissolved in alcoholic solutions were less than 1 mg/cm2/h. Whereas the fluxes of HKL encapsulated in the vesicles were about three times higher than that of HKL in the alcoholic solutions. CONCLUSION: The vesicles could be used for the hair growth promotion.
Subject(s)
Methylamines/pharmacokinetics , Monoterpenes/pharmacokinetics , Skin Absorption/physiology , Stearic Acids/pharmacokinetics , Tropolone/analogs & derivatives , Administration, Cutaneous , Animals , Chlorides/administration & dosage , Chlorides/pharmacokinetics , Drug Carriers/administration & dosage , Drug Carriers/pharmacokinetics , Female , Methylamines/administration & dosage , Mice , Mice, Hairless , Monoterpenes/administration & dosage , Permeability/drug effects , Skin/drug effects , Skin/metabolism , Skin Absorption/drug effects , Stearic Acids/administration & dosage , Tropolone/administration & dosage , Tropolone/pharmacokineticsABSTRACT
BACKGROUND: To evaluate the combined effects of high pressure extraction (HPE) and probiotic fermentation on the antimicrobial and antimutagenic activities, Berberis koreana was subjected to 500 MPa for 30 min and then fermented with Bifidobacterium longum B6 (HPE-BLF) and Lactobacillus paracasei (HPE-LPF) at 37 °C for 6 days. RESULTS: The phenol content was significantly increased to 228 mg GAE g(-1) by the HPE compared to the conventional extraction (CE, 188 mg GAE g(-1)). The HPE-BLF and HPE-LPF showed the highest antimicrobial activity (MIC < 4 mg mL(-1)) against ß-lactam antibiotic sensitive and resistant Staphylococcus aureus. No significant mutagenic effect was observed for CE, HPE, HPE-BLF, and HPE-LPF extracts. The highest antimutagenic activities against frame-shift mutant Salmonella typhimurium were observed at the HPE-LPF (82%), followed by the HPE-BLF (77%). CONCLUSION: The combined HPE and fermentation process could be used as an alternative extraction method for improving the extraction efficacy of medicinal plants. The results will provide pharmaceutically useful information and potential direction for finding new drug sources from medicinal plants.