ABSTRACT
It has been increasingly recognized that CWIN (cell wall invertase) and sugar transporters including STP (sugar transport protein) and SWEET (sugar will eventually be exported transporters) play important roles in plant-pathogen interactions. However, the information available in the literature comes from diverse systems and often yields contradictory findings and conclusions. To solve this puzzle, we provide here a comprehensive assessment of the topic. Our analyses revealed that the regulation of plant-microbe interactions by CWIN, SWEET, and STP is conditioned by the specific pathosystems involved. The roles of CWINs in plant resistance are largely determined by the lifestyle of pathogens (biotrophs versus necrotrophs or hemibiotrophs), possibly through CWIN-mediated salicylic acid or jasmonic acid signaling and programmed cell death pathways. The up-regulation of SWEETs and STPs may enhance or reduce plant resistance, depending on the cellular sites from which pathogens acquire sugars from the host cells. Finally, plants employ unique mechanisms to defend against viral infection, in part through a sugar-based regulation of plasmodesmatal development or aperture. Our appraisal further calls for attention to be paid to the involvement of microbial sugar metabolism and transport in plant-pathogen interactions, which is an integrated but overlooked component of such interactions.
Subject(s)
Sugars , beta-Fructofuranosidase , Biological Transport , Gene Expression Regulation, Plant , Plant Proteins/genetics , Plant Proteins/metabolism , Plants/metabolism , Sugars/metabolism , beta-Fructofuranosidase/metabolismABSTRACT
BACKGROUND: We investigated whether glycemic control affects the relation between endothelial dysfunction and coronary artery disease in patients with type 2 diabetes mellitus (T2DM). METHODS: In 102 type 2 diabetic patients with stable angina, endothelial function was evaluated using brachial artery flow-mediated dilation (FMD) with high-resolution ultrasound, and significant stenosis of major epicardial coronary arteries (≥ 50% diameter narrowing) and degree of coronary atherosclerosis (Gensini score and SYNTAX score) were determined. The status of glycemic control was assessed by blood concentration of glycated hemoglobin (HbA1c). RESULTS: The prevalence of significant coronary artery stenosis (67.9% vs. 37.0%, P = 0.002) and degree of coronary atherosclerosis (Gensini score: 48.99 ± 48.88 vs. 15.07 ± 21.03, P < 0.001; SYNTAX score: 15.88 ± 16.36 vs. 7.28 ± 10.54, P = 0.003) were higher and FMD was lower (6.03 ± 2.08% vs. 6.94 ± 2.20%, P = 0.036) in diabetic patients with poor glycemic control (HbA1c ≥ 7.0%; n = 56) compared to those with good glycemic control (HbA1c < 7.0%; n = 46). Multivariate regression analysis revealed that tertile of FMD was an independent determinant of presence of significant coronary artery stenosis (OR = 0.227 95% CI 0.056-0.915, P = 0.037), Gensini score (ß = - 0.470, P < 0.001) and SYNTAX score (ß = - 0.349, P = 0.004) in diabetic patients with poor glycemic control but not for those with good glycemic control (P > 0.05). CONCLUSION: Poor glycemic control negatively influences the association of endothelial dysfunction and coronary artery disease in T2DM patients.
Subject(s)
Blood Glucose/drug effects , Brachial Artery/physiopathology , Coronary Angiography , Coronary Artery Disease/diagnostic imaging , Coronary Stenosis/diagnostic imaging , Diabetes Mellitus, Type 2/drug therapy , Endothelium, Vascular/physiopathology , Glycemic Control , Hypoglycemic Agents/therapeutic use , Vasodilation , Aged , Biomarkers/blood , Blood Glucose/metabolism , Brachial Artery/diagnostic imaging , Cross-Sectional Studies , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/diagnosis , Diabetes Mellitus, Type 2/physiopathology , Female , Glycated Hemoglobin/metabolism , Humans , Male , Middle Aged , Predictive Value of Tests , Severity of Illness Index , Treatment OutcomeABSTRACT
BACKGROUND: Sulfation of tyrosine, yielding O-sulfotyrosine, is a common but fixed post-translational modification in eukaryotes. Patients with increased circulating O-sulfotyrosine levels experience a faster decline in renal function with progression to end-stage renal disease (ESRD). In the present study, we measured serum O-sulfotyrosine levels in individuals with chronic kidney disease (CKD) and acute kidney injury (AKI) to explore its ability to differentiate AKI from CKD. METHODS: A total of 135 patients (20 with AKI and 115 with CKD) were recruited prospectively for liquid chromatography-mass spectrometry assessment of circulating O-sulfotyrosine. We also studied C57BL/6 mice with CKD after 5/6 nephrectomy (Nx). Blood samples were drawn from the tail vein on Day 1, 3, 5, 7, 14, 30, 60, and 90 after CKD. Serum separation and characterization of creatinine, blood urea nitrogen (BUN), and O-sulfotyrosine was performed. Thus, the time-concentration curves of the O-sulfotyrosine level demonstrate the variation of kidney dysfunction. RESULTS: The serum levels of O-sulfotyrosine were markedly increased in patients with CKD compared with AKI. Median O-sulfotyrosine levels in CKD patients versus AKI, respectively, were as follows:243.61 ng/mL(interquartile range [IQR] = 171.90-553.86) versus 126.55 ng/mL (IQR = 48.19-185.03, P = 0.004). In patients with CKD, O-sulfotyrosine levels were positively correlated with creatinine, BUN, and Cystatin C (r = 0.63, P < 0.001; r = 0.49, P < 0.001; r = 0.61, P < 0.001, respectively) by the multivariate linear regression analysis (ß = 0.71, P < 0.001; ß = 0.40, P = 0.002; ß = 0.73, P < 0.001, respectively). However, this association was not statistically significant in patients with AKI (r = - 0.17, P = 0.472; r = 0.11, P = 0.655; r = 0.09, P = 0.716, respectively). The receiver operating characteristic (ROC) analysis illustrated that the area under the curve was 0.80 (95% confidence interval [CI] 0.71-0.89; P < 0.001) and the optimal cut-off value of serum O-sulfotyrosine suggesting AKI was < 147.40 ng/mL with a sensitivity and specificity of 80.90 and 70.00% respectively. In animal experiments, serum levels of O-sulfotyrosine in mice were elevated on Day 7 after 5/6 nephrectomy (14.89 ± 1.05 vs. 8.88 ± 2.62 ng/mL, P < 0.001) until Day 90 (32.65 ± 5.59 vs. 8.88 ± 2.62 ng/mL, P < 0.001). CONCLUSION: Serum O-sulfotyrosine levels were observed correlated with degrading renal function and in CKD patients substantially higher than those in AKI patients. Thus serum O-sulfotyrosine facilitated the differential diagnosis of AKI from CKD.
Subject(s)
Acute Kidney Injury/blood , Acute Kidney Injury/diagnosis , Renal Insufficiency, Chronic/blood , Renal Insufficiency, Chronic/diagnosis , Tyrosine/analogs & derivatives , Aged , Animals , Diagnosis, Differential , Female , Humans , Male , Mice , Mice, Inbred C57BL , Middle Aged , Tyrosine/bloodABSTRACT
BACKGROUND: Coronary artery ectasia (CAE) is an angiographic finding of abnormal coronary dilatation. Inflammation plays a major role in all phases of atherosclerosis. We investigated the relationship between CAE and serum high-sensitivity C-reactive protein (hs-CRP) and interleukin-6 (IL-6) levels to test our hypothesis that patient age is associated with the efficacy of anti-inflammatory therapy for CAE. METHODS: We conducted a prospective analysis of 217 patients with CAE treated at the Department of Cardiology, Shanghai East Hospital, Ji'an Campus and the Baoshan People's Hospital, from January 1, 2015 to July 30, 2019. Baseline data of patients, including sex; age; and history of hypertension, hyperlipidemia, and diabetes, were collected from patient medical records. Study participants were grouped by age as follows: CAE-A (n = 60, age ≤ 50 years), CAE-B (n = 83, 50 years
Subject(s)
Anti-Inflammatory Agents/therapeutic use , Coronary Artery Disease/drug therapy , Coronary Vessels/drug effects , Rosuvastatin Calcium/therapeutic use , Age Factors , Aged , Biomarkers/blood , C-Reactive Protein/metabolism , Case-Control Studies , China , Coronary Artery Disease/blood , Coronary Artery Disease/diagnostic imaging , Coronary Vessels/diagnostic imaging , Dilatation, Pathologic , Female , Humans , Inflammation Mediators/blood , Interleukin-6/blood , Male , Middle Aged , Prospective Studies , Time Factors , Treatment OutcomeABSTRACT
Reduced cell wall invertase (CWIN) activity has been shown to be associated with poor seed and fruit set under abiotic stress. Here, we examined whether genetically increasing native CWIN activity would sustain fruit set under long-term moderate heat stress (LMHS), an important factor limiting crop production, by using transgenic tomato (Solanum lycopersicum) with its CWIN inhibitor gene silenced and focusing on ovaries and fruits at 2 d before and after pollination, respectively. We found that the increase of CWIN activity suppressed LMHS-induced programmed cell death in fruits. Surprisingly, measurement of the contents of H2O2 and malondialdehyde and the activities of a cohort of antioxidant enzymes revealed that the CWIN-mediated inhibition on programmed cell death is exerted in a reactive oxygen species-independent manner. Elevation of CWIN activity sustained Suc import into fruits and increased activities of hexokinase and fructokinase in the ovaries in response to LMHS Compared to the wild type, the CWIN-elevated transgenic plants exhibited higher transcript levels of heat shock protein genes Hsp90 and Hsp100 in ovaries and HspII17.6 in fruits under LMHS, which corresponded to a lower transcript level of a negative auxin responsive factor IAA9 but a higher expression of the auxin biosynthesis gene ToFZY6 in fruits at 2 d after pollination. Collectively, the data indicate that CWIN enhances fruit set under LMHS through suppression of programmed cell death in a reactive oxygen species-independent manner that could involve enhanced Suc import and catabolism, HSP expression, and auxin response and biosynthesis.
Subject(s)
Cell Wall/enzymology , Fruit/enzymology , Plant Proteins/metabolism , Reactive Oxygen Species/metabolism , beta-Fructofuranosidase/metabolism , Apoptosis/genetics , Catalase/genetics , Catalase/metabolism , Flowers/genetics , Flowers/metabolism , Fructokinases/genetics , Fructokinases/metabolism , Fruit/genetics , Gene Expression Regulation, Plant , Heat-Shock Proteins/genetics , Heat-Shock Proteins/metabolism , Hexokinase/genetics , Hexokinase/metabolism , Hot Temperature , Hydrogen Peroxide/metabolism , Solanum lycopersicum/enzymology , Solanum lycopersicum/genetics , Solanum lycopersicum/metabolism , Malondialdehyde/metabolism , Plant Proteins/genetics , Plants, Genetically Modified , RNA Interference , Reverse Transcriptase Polymerase Chain Reaction , Stress, Physiological , beta-Fructofuranosidase/geneticsABSTRACT
BACKGROUND: Yunnan Province is located in southwestern China and neighbors the Southeast Asian countries, all of which are dengue-endemic areas. In 2000-2013, sporadic imported cases of dengue fever (DF) were reported almost annually in Yunnan Province. During 2013-2015, we confirmed that a large-scale indigenous DF outbreak emerged in cities of Yunnan Province near the China-Myanmar-Laos border. METHODS: Epidemiological characteristics of DF in Yunnan Province during 2013-2015 were evaluated by retrospective analysis. A total of 232 dengue virus (DENV)-positive sera were randomly collected for sequence analysis of the capsid/premembrane region of DENV from patients with DF in Yunnan Province. The envelope gene of DENV isolates was also amplified and sequenced. Phylogenetic analyses were performed using the neighbor-joining method with the Tajima-Nei model. RESULTS: Phylogenetically, all DENV-positive samples could be classified into DENV-1 genotype I and DENV-2 Asian I genotype during 2013-2015 and DENV-4 genotype I in 2015 from Ruili City; and DENV-3 genotype II in 2013 and DENV-2 Cosmopolitan genotype in 2015 from Xishuangbanna Prefecture. CONCLUSIONS: Our results indicated that imported DF from patients from Laos and Myanmar was the primary cause of the DF epidemic in Yunnan Province. Additionally, DENV strains of all four serotypes were identified in indigenous cases in Yunnan Province during the same time period, while the dengue epidemic pattern observed in southwestern Yunnan showed characteristics of a hypoendemic nature: circulation of DENV-1 and DENV-2 over consecutive years.
Subject(s)
Dengue Virus/genetics , Dengue/diagnosis , Dengue/epidemiology , Phylogeny , Adult , Capsid Proteins/genetics , China/epidemiology , Cities , Dengue Virus/isolation & purification , Dengue Virus/pathogenicity , Disease Outbreaks , Epidemics , Female , Genotype , Humans , Laos , Male , Middle Aged , Myanmar , Retrospective Studies , Rural Population , Seasons , Serogroup , Young AdultABSTRACT
Reactive astrocytosis has been considered either beneficial or detrimental effection in neuroinflammatory disease. HSPA12B, a new member belongs to the 70-kDa family of heat shock proteins (HSP70) which could modulate inflammatory response, also shows an connection with the astrocyte activation. Recently, it was reported that Src-Suppressed-C Kinase Substrate (SSeCKS) was detected in heat shock protein A12B (HSPA12B) interacting proteins using a yeast 2-hybrid system. SSeCKS, a major Lipopolysaccharide (LPS) response protein, has been involved in regulating astrocyte activation via production of proinflammatory factor in CNS inflammation. In this study, we found HSPA12B might regulate the expression and activity of SSeCKS to promote astrocyte inflammatory activation and release of inflammatory mediators, such as TNF-α and IL-1ß in spinal cord primary astroglial cultures exposed to LPS treatment. The promoting mechanism of interaction between HSPA12B and SSeCKS on LPS-induced astrocyte activation was mediated via the activation of JNK and p38 signaling pathways but not ERK1/2 MAPK signaling pathway. HSPA12B binded to SSeCKS via its both N terminus consisted of amino acids 1-330 and C terminus consisted of amino acids 1278-1596. And, in vivo, we confirmed the interaction between HSPA12B and SSeCKS of astrocyte activation in the pathogenesis of EAE. The regulatory mechanisms of HSPA12B-SSeCKS interaction may possibly be the key therapeutic strategy of neuroinflammatory disease.
Subject(s)
A Kinase Anchor Proteins/metabolism , Astrocytes/metabolism , Cell Cycle Proteins/metabolism , HSP70 Heat-Shock Proteins/metabolism , Inflammation/metabolism , Animals , Astrocytes/drug effects , Astrocytes/immunology , Cells, Cultured , Female , Guinea Pigs , HEK293 Cells , Humans , Inflammation/immunology , Lipopolysaccharides/pharmacology , Rats , Rats, Inbred LewABSTRACT
Wheeled mobile robots (WMRs) have a wide range of applications in logistics transportation and industrial productions, among which the motion control has always been one of the hot spots in the current WMR researches. However, most of previous designed controllers assumed that the WMR motion had no slippage. Ignoring the slippage factors usually results in a decrease in control performance and even leads to unstable motion. To address such a challenge, a kinematic model with differential flatness is established through dynamic feedback-linearization, which comprehensively considers the multidirectional slippage of mobile robot, including longitudinal and steering slippage. Subsequently, benefited from the one-to-one mapping of states and inputs to flat outputs in differential flat system, an adaptive robust control (ARC) method is proposed to stabilize the system. Different from previous robust control studies, even if the knowledge of the upper bound of system uncertainties is unknown in advance, the proposed adaptive robust controller can still achieve satisfying performance by adaptive estimation of the upper bound of system uncertainties. The effectiveness and feasibility of the proposed method are confirmed by comparative experiments on WMR with slippage disturbance.
ABSTRACT
OBJECTIVE: To explore the clinical efficacies of scleral tunnel sutureless trabeculectomy plus phacoemulsification and intraocular lens implantation in the treatment of glaucoma and cataract. METHODS: A total of 49 cataract patients with glaucoma (52 eyes) from our hospital during the period of February 2009 to June 2012. Their visual acuities were < 0.3 and intraocular pressure was not desirable or in need of various ocular hypotensive drugs. A phaco tunnel knife was employed to prepare a 4 mm × 4 mm scleral tunnel flap with 1/2-2/3 whole layer of sclera depth. Phacoemulsification instrument was used to emulsify cataracts. Foldable intraocular lens was implanted. A trabecular cut of 2 mm × 1.5 mm was made under the scleral tunnel. Tile scleral flap was not sutured and conjunctival flap tightly closed. RESULTS: After a 12-month follow-up, > 0.5 in 42 eyes (80.8%). The intraocular pressure was 11.32 - 17.63 mm Hg (1 mm Hg = 0.133 kPa) and the mean intraocular pressure (13.59 ± 4.26) mm Hg. There was a mean pressure reduction of 11.2 mm Hg from (24.53 ± 5.71) mm Hg pre-operation. Statistical significance existed (t' = 11.073, P < 0.01). The filtering blebs of type I-II was 43 eyes (82.6%) and the mean central anterior chamber 3.28 mm ± 0.42 mm. There was statistical difference (t' = 20.486, P < 0.01) with (1.79 ± 0.31) mm at pre-operation. On 52 eyes, the mean postoperative astigmatism was (1.01 ± 0.62) D versus (1.13 ± 0.74) D at pre-operation. There was no statistical significance (F = 2.0673, P > 0.05). No severe complication occurred in all cases. CONCLUSION: Compared with traditional trabeculectomy and triple procedure, this improved and updated procedure lowers intraocular pressure, improves visual acuity and decreases the occurrences of complications. It is a safe, reasonable, quick and effective treatment for cataract patients with glaucoma.
Subject(s)
Lens Implantation, Intraocular/methods , Sclera/surgery , Trabeculectomy/methods , Aged , Aged, 80 and over , Cataract/complications , Cataract/therapy , Female , Glaucoma/complications , Glaucoma/surgery , Humans , Male , Middle Aged , Phacoemulsification , Treatment OutcomeABSTRACT
This paper studies the energy scheduling for Denial-of-Service (DoS) attack against remote state estimation over multi-hop networks. A smart sensor observes a dynamic system, and transmits its local state estimate to a remote estimator. Due to the limited communication range of the sensor, some relay nodes are employed to deliver data packets from the sensor to the remote estimator, which constitutes a multi-hop network. To maximize the estimation error covariance with energy constraint, a DoS attacker needs to determine the energy level implemented on each channel. This problem is formulated as an associated Markov decision process (MDP), and the existence of an optimal deterministic and stationary policy (DSP) is proved for the attacker. Besides, a simple threshold structure of the optimal policy is obtained, which significantly reduces the computational complexity. Furthermore, an up-to-date deep reinforcement learning (DRL) algorithm, dueling double Q-network (D3QN), is introduced to approximate the optimal policy. Finally, a simulation example illustrates the developed results and verifies the effectiveness of D3QN for optimal DoS attack energy scheduling.
Subject(s)
Algorithms , Wireless Technology , Computer Simulation , Markov Chains , Physical PhenomenaABSTRACT
Most existing approximation-based adaptive control (AAC) approaches for unknown pure-feedback nonaffine systems retain a dilemma that all closed-loop signals are semiglobally uniformly bounded (SGUB) rather than globally uniformly bounded (GUB). To achieve the GUB stability result, this article presents a neuro-adaptive backstepping control approach by blending the mean value theorem (MVT), the barrier Lyapunov functions (BLFs), and the technique of neural approximation. Specifically, we first resort the MVT to acquire the intermediate and actual control inputs from the nonaffine structures directly. Then, neural networks (NNs) are adopted to approximate the unknown nonlinear functions, in which the compact sets for maintaining the approximation capabilities of NNs are predetermined actively through the BLFs. It is shown that, with the developed neuro-adaptive control scheme, global stability of the resulting closed-loop system is ensured. Simulations are conducted to verify and clarify the developed approach.
ABSTRACT
BACKGROUND AND AIMS: Coordination of sugar transport and metabolism between developing seeds and their enclosing fruit tissues is little understood. In this study the physiological mechanism is examined using two genotypes of asparagus bean (Vigna unguiculata ssp. sesquipedialis) differing in pod wall and seed growth rates. Pod growth dominates over seed growth in genotype 'Zhijiang 121' but not in 'Zhijiang 282' in which a 'bulging pod' phenotype is apparent from 8 d post-anthesis (dpa) onward. METHODS: Seed and pod wall growth rates and degree of pod-bulging were measured in the two genotypes together with assays of activities of sucrose-degrading enzymes and sugar content in pod wall and seed and evaluation of cellular pathways of phloem unloading in seed coat using a symplasmic fluorescent dye, 5(6)-carboxyfluorescein (CF). KEY RESULTS: Activities of cell wall, cytoplasmic and vacuolar invertases (CWIN, CIN and VIN) were significantly smaller in pod walls of '282' than in '121' at 10 dpa onwards. Low INV activities were associated with weak pod wall growth of '282'. In seed coats, CF was confined within the vasculature in '282' but moved beyond the vasculature in '121', indicating apoplasmic and symplasmic phloem unloading, respectively. Higher CWIN activity in '282' seed coats at 6-8 dpa correlated with high hexose concentration in embryos and enhanced early seed growth. However, CWIN activity in '282' decreased significantly compared with '121' from 10 dpa onwards, coinciding with earlier commencement of nuclei endoreduplication in their embryos. CONCLUSIONS: The study shows genotypic differences between 'bulging pod' and 'non-bulging' phenotypes of asparagus bean in sucrose metabolism in relation to the pathway of phloem unloading in developing seed coats, and to pod and seed growth. Low INV activity in pod wall corresponds to its shortened and weak growth period; by contrast, the apoplasmic path in the seed coat is associated with high CWIN activity and strong early seed growth.
Subject(s)
Fabaceae/embryology , Seeds/growth & development , beta-Fructofuranosidase/metabolism , Biological Transport , Fabaceae/enzymology , Fabaceae/growth & development , GenotypeABSTRACT
Three heat shock protein 70 family transcripts, named PcHsp70-1, PcHsp70-2 and PcHsp70-3, were isolated from the citrus red mite, Panonychus citri. PcHsp70-1, PcHsp70-2, and PcHsp70-3 contained an open reading frame of 1977, 1968, and 2028 nucleotides that encoded 658, 655 and 675 amino acid residues, respectively. Comparison of deduced amino acid sequences of PcHsp70-1 and PcHsp70-2 showed 86.34% identity, while the amino acid sequence of PcHsp70-3 was only 57.39 and 58.75% identical to that of PcHsp70-1 and PcHsp70-2, respectively. Sequences and phylogenetic analyses suggested that PcHsp70-1 and PcHsp70-2 were cytosolic Hsps, whereas PcHsp70-3 was located in ER (endoplasmic reticulum). To accurately validate mRNA expression profiles of the three Hsp70s under thermal stress conditions, seven housekeeping genes were evaluated. Alpha-tubulin and RpII were selected as optimal endogenous references for cold shock and heat shock conditions, respectively. Real-time quantitative RT-PCR revealed that only the mRNA expression of PcHsp70-2 was up-regulated under heat shocks, and all of the three Hsp70s were constitutively expressed under cold shocks. The results suggest that the three Hsp70s were more critical to coping with heat than cold shocks.
Subject(s)
Citrus/parasitology , Gene Expression Profiling , HSP70 Heat-Shock Proteins/genetics , Heat-Shock Response/genetics , Pigmentation/genetics , Amino Acid Sequence , Animals , Base Sequence , Cold Temperature , Gene Expression Regulation , HSP70 Heat-Shock Proteins/chemistry , HSP70 Heat-Shock Proteins/metabolism , Mites/genetics , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Reference Standards , Sequence Analysis, DNA , SoftwareABSTRACT
In this article, the problem of adaptive tracking control is tackled for a class of high-order nonlinear systems. In contrast to existing results, the considered system contains not only unknown nonlinear functions but also unknown rational powers. By utilizing the fuzzy approximation approach together with the barrier Lyapunov functions (BLFs), we present a new adaptive tracking control strategy. Remarkably, the BLFs are employed to determine a priori the compact set for maintaining the validity of fuzzy approximation. The primary advantage of this article is that the developed controller is independent of the powers and can be capable of ensuring global stability. Finally, two illustrative examples are given to verify the effectiveness of the theoretical findings.
ABSTRACT
OBJECTIVE: To evaluate the CT imaging characteristics of incomplete and complete myocardial bridges-mural coronary artery (MB-MCA). METHODS: Fifty subjects with dual source coronary CT angiography (DSCTA) evidenced MB were included. The subjects were divided into incomplete MB-MCA and complete MB-MCA groups. The diameter of MCA in best systole phase and diastole phase, the MCA stenosis rate, the presence of atheromatous change proximal to the MB were evaluated. RESULTS: There were 58 MB, the average length was (2.02 ± 1.02) cm, 23 were incomplete MB and 35 were complete MB. Thirty-two MB were in the middle segments of left anterior descending artery (55.2%); 17 MB were in the distal segment of the left anterior descending artery (29.3%); 1 MB was in the proximal segment of left anterior descending artery; 3 MB in diagonal branch; 4 MB in obtuse marginal branch, 1 MB in distal right coronary artery. It was statistically significant difference between the incomplete MB-MCA and the complete MB-MCA of the diameter change in diastole and systole phase [(1.93 ± 0.49) mm, (1.71 ± 0.45) mm vs. (2.21 ± 0.41) mm, (1.63 ± 0.52) mm, P = 0.008] and stenosis rate (10.38% ± 20.2% vs. 25.12% ± 21.02%, P = 0.01). Atherosclerotic finding was evidenced in 8 incomplete MB (34.78%) and 15 complete MB (42.86%) at the proximal vessel of mural coronary artery (P > 0.05). CONCLUSION: DSCTA can vividly display the incomplete and complete myocardial MB, accurately evaluate the shape change of MB-MCA in diastole and systole phase and detect the atherosclerotic change in the proximal vessel of MB.
Subject(s)
Atherosclerosis/diagnostic imaging , Coronary Angiography , Myocardial Bridging/diagnostic imaging , Adult , Aged , Aged, 80 and over , Coronary Vessels , Female , Humans , Male , Middle Aged , Retrospective Studies , Tomography, X-Ray ComputedABSTRACT
BACKGROUND: Primary anaplastic large cell lymphoma of the lung represents a diagnostic challenge due to diverse manifestations and non-specific radiological findings, particularly in cases that lack extra-pulmonary manifestations and lung biopsy. CASE SUMMARY: A 40-year-old woman presented with a 6-d history of fever, dry coughing, and dyspnea. Her white blood cell count was 20100/mm3 with 90% neutrophils. PaO2 was 60 mmHg and SaO2 was 90% when breathing ambient air. Chest computed tomography (CT) identified a solid nodule, 15 mm in diameter, with a poorly defined boundary in the upper right lung, and several smaller solid nodules throughout both lungs. Pulmonary artery CT and subsequent bedside X-ray showed diffuse patchy shadows throughout both lungs. Repeated cultures of blood samples and alveolar lavage failed to identify any pathogen. Due to the mismatch between clinical and imaging features, we conducted a bone marrow biopsy, and the results showed proliferation along all three lineages but no atypical or malignant cells. The patient received empirical antibacterial, antiviral, and antifungal treatments, as well as corticosteroids. The patient's condition deteriorated rapidly despite treatment. The patient died 6 d after hospitalization due to respiratory failure. Post-mortem lung biopsy failed to show inflammation but identified widespread infiltration of alveolar septum by anaplastic lymphoma kinase (ALK)-positive anaplastic cells. CONCLUSION: ALK-positive anaplastic large cell lymphoma could present as a primary pulmonary disease without extra-pulmonary manifestations.
ABSTRACT
Tumor-associated macrophages (TAMs) regulate tumor immunity. Previous studies have shown that the programmed cell death protein 1 (PD-1)-positive TAMs have an M2 macrophage phenotype. CD68 is a biomarker of TAMs and is considered to be a poor prognostic marker of several malignancies. Our results show that PD-1-positive TAMs can be a negative survival indicator in patients with muscle-invasive bladder cancer (MIBC), and that the mechanistic effects could result due to a combination of PD-1 and CD68 activity. We analyzed 22 immune cell types using data from 402 patients with MIBC from the TCGA database, and found that a high immune score and M2 TAMs were strongly associated with poor clinical outcomes in patients with MIBC. Further, we analyzed resected samples from 120 patients with MIBC and found that individuals with PD-1-positive TAMs showed a reduction in 5-year overall survival and disease-free survival. Additionally, PD-1-positive TAMs showed a significant association with higher programmed death-ligand 1 (PD-L1) expression, the Ki67 index, the pT stage and fewer CD8-positive T cells. Through the co-immunoprecipitation (co-IP) assay of THP-1 derived macrophages, we found that CD68 can bind to PD-1. The binding of CD68 and PD-1 can induce M2 polarization of THP-1 derived macrophages and promote cancer growth. The anti-CD68 treatment combined with peripheral blood mononuclear cells (PBMC) showed obvious synergy effects on inhibiting the proliferation of T24 cells. Together, these results indicate for the first time that CD68/PD-1 may be a novel target for the prognosis of patients with MIBC.
ABSTRACT
Four housekeeping genes named Lbbeta-Actin1, Lbbeta-Actin2, Lbalpha-Tubulin, and LbGapdh were cloned from Liposcelis bostrychophila using the combined techniques of reverse transcriptase-PCR (RT-PCR) with rapid amplification of cDNA ends (RACE). The GenBank accession numbers were FJ196622, FJ447483, FJ595242, and FJ595241, respectively. The full-length cDNA of Lbbeta-Actin1 was a 1,772 bp sequence with an open reading frame (ORF) encoded 376 amino acids, while Lbbeta-Actin2 was 1,350 bp in length containing an ORF encoded 376 amino acids. Furthermore, the 1,565 bp cDNA of Lbalpha-Tubulin had an ORF of 1,350 bp encoding 450 amino acids. And LbGapdh possessed an ORF of 333 amino acids. Sequences analysis and phylogenetic trees generated from the nucleotide sequences of their coding regions revealed a relationship that was closer to other insects than to mammals. The four genes together with 18S rRNA were quantified for transcription stability in L. bostrychophila, and the geNorm software ranked from the most to least were Lbbeta-Actin1 > LbGapdh > Lbalpha-Tubulin > Lbbeta-Actin2 > Lb18S rRNA for deltamethrin induction, while ranked Lbbeta-Actin1 > Lbalpha-Tubulin > Lbbeta-Actin2 > LbGapdh > Lb18S rRNA for the different developmental stages.
Subject(s)
Gene Expression Regulation, Developmental , Genes, Insect , Insecta/genetics , Insecticides/pharmacology , Up-Regulation/drug effects , Animals , Gene Expression Profiling , Gene Expression Regulation, Developmental/drug effects , Genes, Insect/drug effects , Insect Control/methods , Insect Control/standards , Insecta/drug effects , Insecta/growth & development , Insecticide Resistance/genetics , Life Cycle Stages/drug effects , Life Cycle Stages/genetics , Nitriles/pharmacology , Phylogeny , Pyrethrins/pharmacology , Reference Standards , Sequence Analysis, DNAABSTRACT
The citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae), is regarded as one of the most important citrus pests in many countries, such as Japan, Spain, and China. In this study, the mitochondrial cytochrome oxidase subunit I gene was used to analyze genetic diversity and population structure of 15 P. citri populations collected from three citrus (Citrus spp.) planting regions of China. Our analysis found that these populations had relatively low genetic diversities. Bayesian tree and haplotype network showed that the 22 haplotypes of P. citri formed two lineages with low Bayesian posterior probabilities (0.55 and 0.63), and haplotypes in a sample were scattered throughout the Bayesian tree, indicating that there may be no significant genealogical structure among populations. However, when Yuxi and Danjiangkou populations (both without H7 haplotype) were excluded from the analysis, analysis of molecular variance found a weak, but significant, geographic structuring. Mantel test indicated a significant and positive correlation between genetic and geographical distances, reflecting certain degree of isolation by distance. The genetic differentiation based on pairwise F(ST) was not significant between most populations, and some F(ST) were even negative, indicating remarkable gene flow among these populations. The weak population structure of P. citri in this study was probably influenced by high gene flow between some populations due to long-distance dispersal of this species, which may be largely relied on the movement of plants between populations. The mismatch distribution analysis showed that no signal of population growth, but the Fu's F(S) value was significantly negative for total populations and the star-like shape of haplotype network, suggesting a history of population expansion of P. citri in China.
Subject(s)
Electron Transport Complex IV/genetics , Genetic Variation , Genetics, Population , Tetranychidae/genetics , Animals , China , Haplotypes , Phylogeny , Sequence Analysis, DNAABSTRACT
In this article, a globally stable adaptive control strategy for uncertain strict-feedback systems is proposed within predefined neural network (NN) approximation sets, despite the presence of unknown system nonlinearities. In contrast to the conventional adaptive NN control results in the literature, a primary benefit of the developed approach is that the barrier Lyapunov function is employed to predefine the compact set for maintaining the validity of NN approximation at each step, thus accomplishing the global boundedness of all the closed-loop signals. Simulation results are performed to clarify the effectiveness of the proposed methodology.