1.
Opt Express
; 16(25): 20258-65, 2008 Dec 08.
Article
in English
| MEDLINE
| ID: mdl-19065164
ABSTRACT
We combine single molecule fluorescence orientation imaging with single-pair fluorescence resonance energy transfer microscopy, using a total internal reflection microscope. We show how angles and FRET efficiencies can be determined for membrane proteins at the single molecule level and provide data from the epidermal growth factor receptor system in cells.