ABSTRACT
In order to grow, solid tumors need to develop new blood vessels. Neoplastic cells secrete growth factors that stimulate angiogenesis and tumor growth. Since Carrageenan acts as in vitro blocking agent which interferes with growth factor-receptor binding, we tested its action in vivo in order to analyze its growth inhibition capability in an experimental murine fibrosarcoma model. Indomethacin was used as a non-steroidal anti-inflammatory agent to neutralize the inflammatory action of Carrageenan. A murine fibrosarcoma was induced with methylcholanthrene in Balb/c mice and maintained by serial passage of tumor cells in mice of the same strain. Tumor volume was evaluated measuring two dimensions and applying the formula V = 0.4 x d2 x D. The mice with tumors were separated into groups; one of them was used as control and the other ones were treated with Indomethacin, Carrageenan and Carrageenan-Indomethacin. Tumor volume was compared between groups using the Student t Test. We demonstrated that Carrageenan and Indomethacin inhibit tumor growth. The inhibitory action of Carrageenan is significantly higher than the antitumoral effect of either Indomethacin or Carrageenan-Indomethacin.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/antagonists & inhibitors , Carrageenan/pharmacology , Fibrosarcoma/drug therapy , Indomethacin/antagonists & inhibitors , Receptors, Growth Factor/drug effects , Animals , Carcinogens , Carrageenan/therapeutic use , Female , Fibrosarcoma/chemically induced , Fibrosarcoma/metabolism , Male , Methylcholanthrene , Mice , Mice, Inbred BALB C , Neoplastic ProcessesABSTRACT
The present study evaluated the effect of sodium saccharin on mouse tracheal epithelium, in relation to its possible structural alterations. Mice of the C3H strain were fed with standard pellets supplemented with sodium saccharin for 180 days. At that time the mice were sacrificed and their trachea were processed for transmission electronic microscopy. We demonstrated that sodium saccharin produces alterations in cellular surface cilia, with cytoplasmic excrescences and ciliary malformations. These results suggest that sodium saccharin is not an innocuous sweetener and that it may cause structural alterations in epithelial tissues.
Subject(s)
Saccharin/pharmacology , Trachea/drug effects , Animals , Epithelium/drug effects , Male , MiceABSTRACT
Saccharin and cyclamates have been proved to cause organic damage. This work attempt to describe drug-induced changes brought about by drugs in 1/1000 saccharin and cyclamate-fed rats for 90 days. The ultrastructural findings show: microvilli hypertrophy; membranous mitochondrial increase in absorptive cells; and secretion changes in calciform cells. Such changes are cell-response phenomena to interference or mutagenic action on nuclear DNA or on cytoplasmatic metabolism.
Subject(s)
Cyclamates/adverse effects , Intestine, Large/drug effects , Intestine, Large/ultrastructure , Saccharin/adverse effects , Animals , Cell Membrane/drug effects , Cell Membrane/ultrastructure , Cyclamates/toxicity , Diet , Female , Male , Mice , Mice, Inbred C3H , Saccharin/toxicityABSTRACT
Sodium saccharin has found to be a tumoral promoter in the rat's bladder epithelium, property not demonstrated in humans. Nevertheless, at present there's no references on the possible alterations produced by sodium saccharin in the epithelium of the mice colon. In this work we describe the alterations produced by low doses of sodium saccharin in the epithelium of the mice colon. The changer produced by sodium saccharin consist in pleomorphic microvill with variations of form, length diameter and curvature and demonstrate by transmission electron microscopy.
Subject(s)
Colon/drug effects , Intestinal Mucosa/drug effects , Saccharin/adverse effects , Sweetening Agents/adverse effects , Animals , Colon/pathology , Female , Intestinal Absorption/drug effects , Intestinal Mucosa/pathology , Intestinal Mucosa/ultrastructure , Intestinal Neoplasms/chemically induced , Male , Mice , Microscopy, Electron , Microvilli , SodiumABSTRACT
The aim of the present paper is to show lead toxicity and cell deposition of concentrations lower than those regarded as toxic on an experimental model with rats C3Hs., forty five grams-rats were used. A standard diet was administered together with water ad-libitium, containing very low doses of lead acetate which was constantly administered and at fixed periods. Light and electron microscopy were used to study the liver and the spleen. These organs are considered to harbour a great amount of constant macrophages with phagocytic function. The findings showed lesions and lead deposits which confirmed the causative agent as well as its toxic contaminating action.
Subject(s)
Lead Poisoning/pathology , Liver/pathology , Spleen/pathology , Animals , Mice , Mice, Inbred C3HABSTRACT
Se ha demostrado que la sacarina y los ciclamatos provocan lesiones en el organismo. Describimos en este trabajo las alteraciones producidas por estas drogas en ratones alimentados con una solución de sacarina y ciclamato al 1/1000, durante 90 días. El estudio ultraestructural revela: Hipertrofia microvellositaria e incremento membranoso mitocondrial en las células absortivas y alteraciones en la secreción de células calciformes. Estas modificaciones son fenómenos de respuesta celular por interferencia o acción mutágena sobre el ADN nuclear o metabolismo citoplasmático. Los autores agradecen a la señora Carolina S. de Santolaya por el apoyo técnico realizado. Esta investigación fue apoyada financieramente por el Consejo de Investigación de la Unviersidad Nacional de Tucumán
Subject(s)
Animals , Male , Female , Mice , Cyclamates/adverse effects , Intestine, Large/ultrastructure , Saccharin/adverse effects , Sweetening Agents/administration & dosage , Cyclamates/toxicity , Diet , Saccharin/toxicityABSTRACT
Se ha comprobado que la sacarina de sodio es un promotor tumoral del epitelio de la vegija de rata, propiedad no demostrada en el ser humano y actualmente discutida. En este trabajo se describen las alteraciones que produce este edulcorante en el epitelio del colon descendente de ratones cepa C3H, cuando es adicional al alimento en bajas dosis. Se muestra por microscopia electrónica de transmisión, que la sacarina de sodio produce en las células absortivas del epitelio del colon un pleomorfismo microvellositario constituido por microvellosidades de diferente forma, longitud, diámetro y curvatura.