ABSTRACT
The purpose of this study was to evaluate the histopathological effects of camphorquinone (CQ) and 9-fluorenone (9F) with or without visible light (VL) irradiation on the oral mucous membranes of mice. VL irradiation resulted in a higher degree of tissue damage after CQ or 9F application, particularly the latter. Necrosis and apoptosis were responsible for the tissue damage after application of either agent in the presence of VL irradiation.
Subject(s)
Dental Materials/toxicity , Fluorenes/toxicity , Light , Mouth Mucosa/drug effects , Terpenes/toxicity , Animals , Apoptosis/drug effects , Cell Nucleus/drug effects , Cell Nucleus/ultrastructure , Coloring Agents , Cytoplasmic Granules/drug effects , Cytoplasmic Granules/ultrastructure , Cytoplasmic Vesicles/drug effects , Cytoplasmic Vesicles/ultrastructure , Dental Materials/radiation effects , Edema/chemically induced , Edema/pathology , Epithelial Cells/drug effects , Fluorenes/radiation effects , Fluorescent Dyes , In Situ Nick-End Labeling , Materials Testing , Mice , Mice, Inbred ICR , Mouth Mucosa/pathology , Necrosis , Stomatitis/chemically induced , Stomatitis/pathology , Terpenes/radiation effectsABSTRACT
The purpose of this study was to evaluate the histopathological effects of eugenol (EUG) and iso-eugenol (IsoEUG)--with or without visible light (VL) irradiation--on oral mucous membranes. Oral mucous membranes of mice were applied with three agents, EUG, IsoEUG, and aceton (as the control) in the absence or presence of VL irradiation. VL irradiation resulted in more tissue damage for EUG- or IsoEUG-treated mucosa compared to corresponding compounds without VL irradiation, and that damage under IsoEUG treatment was greater than that under EUG treatment. Necrosis, but not apoptosis, was preferentially expressed in EUG- or IsoEUG-treated mucous membranes in the presence of VL irradiation.
Subject(s)
Eugenol/toxicity , Light/adverse effects , Mouth Mucosa/drug effects , Mouth Mucosa/radiation effects , Animals , Eugenol/analogs & derivatives , In Situ Nick-End Labeling , Mice , Mice, Inbred ICR , NecrosisABSTRACT
The purpose of this study was to evaluate the histopathological effects of curcumin and capsaicin, with or without visible light (VL) irradiation for 5 min, on the oral mucous membrane in mice. Capsaicin-treated, but not curcumin-treated, buccal epithelium exhibited slight tissue damage; VL irradiation caused excessive tissue damage, particularly when combined with the former treatment. The TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method demonstrated that both capsaicin and curcumin induced apoptosis, with the apoptotic effect of capsaicin appearing at an early stage of application. VL irradiation increased the number of apoptotic cells, particularly those upon in the capsaicin-treated area. Capsaicin and curcumin acted as photosensitizers exposure to VL, in the presence of oxygen. Curcumin and capsaicin with VL irradiation could thus be used for photodynamic therapy in the clinical setting, especially in precancerous oral diseases.