Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 95
Filter
Add more filters

Country/Region as subject
Publication year range
1.
Ceska Slov Farm ; 72(6): 277-287, 2024.
Article in English | MEDLINE | ID: mdl-38346905

ABSTRACT

INTRODUCTION: The rate of pharmacoresistance among in patients diagnosed with schizophrenia is around 30%. Clozapineis the drug of choice for these patients; however, an adequate response to treatment doesn't always occur. One of the possible augmentation approaches, specifically for non-adherent patients, is the administration of long-acting parenteral antipsychotics. Our goal was to evaluate previous experiences of administering a combination of the atypical antipsychotic clozapine and long-acting injectable antipsychotics to pharmacoresistant patients at the Department of Psychiatry the Czech Republic and to assess the safety and effectiveness of such administration. METHODS: A retrospective evaluation of patient case studies was conducted for those who were hospitalized in the Ward for the therapy of Psychotic disorders between 2016 and 2020 and had a medication history of combining clozapine and depot antipsychotics. RESULTS: Over half of the patients had no illness relapses during the observed period. The clinical manifestation of adverse effects from combination therapy appears low in our patient sample, primarily involving mild and pharmacologically manageable side effects (tachycardia). Only one of the cases recorded neutropenia, which led to discontinuation of clozapine; the patient was maintained on long-acting injectable antipsychotics medication. CONCLUSION: From our findings, it can be inferred that augmenting clozapine with depot antipsychotics is a potential therapeutic intervention that pharmacoresistant patients could benefit from. However, it is essential to emphasize that this therapeutic approach should only be administered after carefully considering the patient's existing treatment. It should be strictly individualized based on the treating physician's or clinical pharmacist's sufficient professional experience.


Subject(s)
Antipsychotic Agents , Clozapine , Schizophrenia , Humans , Antipsychotic Agents/adverse effects , Clozapine/adverse effects , Schizophrenia/drug therapy , Schizophrenia/chemically induced , Schizophrenia, Treatment-Resistant , Mental Health , Retrospective Studies
2.
Rozhl Chir ; 103(6): 232-235, 2024.
Article in English | MEDLINE | ID: mdl-38991788

ABSTRACT

In this article, we present case reports of two patients admitted to the University Hospital in Pilsen for acute abdomen due to a disorder of the passage through the gastrointestinal tract (GIT). Both were indicated for surgery. The patients were diagnosed intraoperatively with rarely occurring cecal volvulus (CV). The findings required an ileocecal resection; nevertheless, both patients fully recovered despite the need the resection.


Subject(s)
Abdomen, Acute , Cecal Diseases , Intestinal Volvulus , Humans , Intestinal Volvulus/surgery , Intestinal Volvulus/diagnostic imaging , Intestinal Volvulus/complications , Abdomen, Acute/etiology , Cecal Diseases/surgery , Cecal Diseases/complications , Cecal Diseases/diagnostic imaging , Cecal Diseases/diagnosis , Male , Ileus/surgery , Ileus/etiology , Ileus/diagnostic imaging , Female , Middle Aged , Aged
5.
Physiol Res ; 72(Suppl 2): S113-S126, 2023 07 31.
Article in English | MEDLINE | ID: mdl-37565416

ABSTRACT

Contemporary society is characterized by rapid changes. Various epidemiological, political and economic crises represent a burden to mental health of nowadays population, which may at least partially explain the increasing incidence of mental disorders, including schizophrenia. Schizophrenia is associated with premature mortality by at least 13-15 years. The leading cause of premature mortality in schizophrenia patients is high incidence of cardiovascular diseases. The specific-cause mortality risk for cardiovascular diseases in schizophrenia patients is more than twice higher as compared to the general population. Several factors are discussed as the factor of cardiovascular diseases development. Intensive efforts to identify possible link between schizophrenia and cardiovascular diseases are made. It seems that sigma 1 receptor may represent such link. By modulation of the activity of several neurotransmitter systems, including dopamine, glutamate, and GABA, sigma 1 receptor might play a role in pathophysiology of schizophrenia. Moreover, significant roles of sigma 1 receptor in cardiovascular system have been repeatedly reported. The detailed role of sigma 1 receptor in both schizophrenia and cardiovascular disorders development however remains unclear. The article presents an overview of current knowledge about the association between schizophrenia and cardiovascular diseases and proposes possible explanations with special emphasis on the role of the sigma 1 receptor.


Subject(s)
Cardiovascular Diseases , Cardiovascular System , Schizophrenia , Humans , Cardiovascular Diseases/diagnosis , Cardiovascular Diseases/epidemiology , Schizophrenia/diagnosis , Schizophrenia/epidemiology
6.
Physiol Res ; 72(S5): S535-S542, 2023 12 29.
Article in English | MEDLINE | ID: mdl-38165757

ABSTRACT

Pressure ulcers (PUs), also known as pressure injuries, are chronic wounds that represent potential lifelong complications. Pressure ulcers of a deep category (III and IV) are often indicated for surgical treatment - debridement and surgical reconstruction. Sharp surgical debridement is widely used in the debridement of PUs; however, the Versajet® hydrosurgery system is becoming an increasingly popular tool for tangential excision in surgery due to its numerous advantages. This work focused on the expression of selected genes, especially those associated with oxidative stress, in PUs debrided by two approaches - sharp surgical debridement and debridement using Versajet® hydrosurgery system. Expression of following genes was evaluated: NFE2L2, ACTA2, NFKB1, VEGFA, MKI67, HMOX1, HMOX2, HIF1A, and SOD2. ACTB and PSMB were used as housekeeping genes. So far, five patients have been enrolled in the study. Preliminary results suggest no significant difference in gene expression with different pressure ulcer treatment approaches except NFE2L2, despite the macroscopic differences. However, the results revealed correlations between the expression of some genes, namely HIF1A and SOD2, VEGFA and SOD2 and VEGFA and HIF1A. These results may indicate a connection between hypoxia, oxidative stress, pressure ulcer healing processes and angiogenesis.


Subject(s)
Pressure Ulcer , Wound Healing , Humans , Wound Healing/genetics , Debridement/methods , Pressure Ulcer/genetics , Pressure Ulcer/surgery , Pilot Projects , Treatment Outcome , Gene Expression , Suppuration
7.
Physiol Res ; 71(S2): S211-S218, 2022 12 31.
Article in English | MEDLINE | ID: mdl-36647909

ABSTRACT

Based on the World Health Organization statistics, cardiovascular diseases represent the major cause of death worldwide. Although a wide range of treatment approaches and pharmaceuticals is available, the therapy is often not effective enough and therefore health risks for the patient persist. Thus, it is still essential to test new drug candidates for the treatment of various pathophysiological conditions related to cardiovascular system. In vivo models represent indispensable part of preclinical testing of such substances. Anesthetized guinea pig as a whole-body model allows to evaluate complex reactions of cardiovascular system to tested substance. Moreover, action potential of guinea pig cardiomyocyte is quite comparable to that of human. Hence, the results from this model are then quite well translatable to clinical medicine. Aim of this paper was to summarize the methodology of this model, including its advantages and/or limitations and risks, based on the effects of two substances with adrenergic activity on the ECG parameters. The model of anesthetized guinea pig proved to be valuable and suitable for testing of drugs with cardiovascular effects.


Subject(s)
Cardiovascular System , Drug Evaluation, Preclinical , Electrocardiography , Myocardial Contraction , Animals , Guinea Pigs , Humans , Cardiovascular System/drug effects , Heart/drug effects , Heart/physiology , Heart Rate , Myocardial Contraction/drug effects , Myocardial Contraction/physiology , Blood Pressure/drug effects , Disease Models, Animal
8.
Physiol Res ; 71(S2): S251-S257, 2022 12 31.
Article in English | MEDLINE | ID: mdl-36647913

ABSTRACT

Gastropathy is one of the most common diseases of the human gastrointestinal tract. Apart from its consequences in the stomach, it is also manifested in other parts of the digestive tract, particularly in the duodenum. The aim of this pilot study was to verify on animal model the empirically observed alleviation of gastropathy symptoms in patients who underwent a drinking treatment of Vincentka natural mineral water during their spa treatment. Sixteen male Wistar rats were included in the study. The animals were randomly divided into two groups: experimental group (E; n=8) and control group (C; n=8). The experimental protocol consisted of three phases: (1) handling phase (7 days); (2) mineral water (E)/tap water (C) administration (7 days); (3) acute gastritis induction (1 day). Twenty-four hours after the induction of acute gastritis, the animals were sacrificed. The collected tissues (stomach and duodenum) and blood were examined by standard histological microscopy, and by immunohistochemical and biochemical methods. Histopathological analysis revealed significantly reduced damage to the gastric mucosa in the experimental group. Significantly different values of blood plasma antioxidant capacity, oxidative stress parameters and blood plasma biochemical parameters were also found. Based on these results, we conclude that the mineral water Vincentka has a positive impact on development and symptoms of acute gastric ulcers.


Subject(s)
Gastritis , Mineral Waters , Humans , Rats , Animals , Male , Rats, Wistar , Pilot Projects , Ulcer , Gastritis/chemically induced , Gastritis/pathology
9.
Physiol Res ; 59 Suppl 1: S51-S58, 2010.
Article in English | MEDLINE | ID: mdl-20626220

ABSTRACT

Three-dimensional electrogram was used for analysis of ischemia manifestation in isolated hearts. Three parameters based on spherical coordination system were used in this study - amplitude of electrical heart vector, its azimuth and elevation. The parameters were presented as a trend. This approach reflected ischemic changes in a manner which can be easily observed and evaluated. Ischemia was analysed in seven isolated hearts of New Zealand white rabbits. It was found that (a) ischemia changes heart electric vector, (b) ischemic preconditioning has a protective effect, and (c) both of these findings can be clearly observed by the proposed method.


Subject(s)
Electrophysiologic Techniques, Cardiac , Image Interpretation, Computer-Assisted , Imaging, Three-Dimensional , Myocardial Ischemia/diagnosis , Vectorcardiography , Action Potentials , Animals , Disease Models, Animal , Ischemic Preconditioning, Myocardial , Models, Cardiovascular , Myocardial Ischemia/prevention & control , Predictive Value of Tests , Rabbits
10.
Physiol Res ; 59 Suppl 1: S65-S69, 2010.
Article in English | MEDLINE | ID: mdl-20626222

ABSTRACT

Bupivacaine is a widely used long-acting local anaesthetic. In clinical practice, a mixture of bupivacaine and lidocaine is often used in order to combine the faster onset of sensory blockade of lidocaine with more profound and longer duration of blockade by bupivacaine. The aim of this study was to compare the cardiotoxicity of large doses of bupivacaine and mixture of bupivacaine with lidocaine in the isolated rat heart and to estimate whether or not the addition of lidocaine in clinically relevant concentration increases bupivacaine-induced toxicity. Experiments were performed on 21 adult male rats divided into three groups: B (6 microg/ml bupivacaine), BL (6 microg/ml bupivacaine and 12 microg/ml lidocaine) and L (12 microg/ml lidocaine). The experiment consisted of three 30 min periods: stabilisation, perfusion and washout. The isolated hearts were perfused according to Langendorff with Krebs-Henseleit solution at constant pressure (80 mmHg) and 37 degrees C (CaCl(2) 1.25 mM) and the heart rate (based on RR interval assessment), PQ and QRS intervals were measured. The present study shows that the mixture of tested anaesthetics - bupivacaine and lidocaine - impairs the intraventricular conduction parameters (QRS interval prolongation) to a lesser extent than bupivacaine itself, and that this effect is marked mainly at the beginning of perfusion.


Subject(s)
Anesthetics, Combined/toxicity , Anesthetics, Local/toxicity , Bupivacaine/toxicity , Heart Conduction System/drug effects , Heart Rate/drug effects , Lidocaine/toxicity , Animals , Heart Conduction System/physiopathology , In Vitro Techniques , Male , Perfusion , Rats , Rats, Wistar , Time Factors
11.
Physiol Res ; 59 Suppl 1: S71-S80, 2010.
Article in English | MEDLINE | ID: mdl-20626223

ABSTRACT

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.


Subject(s)
Electrophysiologic Techniques, Cardiac , Fluorescence , Heart/physiology , Action Potentials , Animals , Artifacts , Fluorescent Dyes , In Vitro Techniques , Perfusion , Predictive Value of Tests , Rabbits , Reproducibility of Results , Signal Processing, Computer-Assisted , Time Factors
12.
Biotechnol Bioeng ; 102(1): 29-37, 2009 Jan 01.
Article in English | MEDLINE | ID: mdl-18683252

ABSTRACT

The aim of this work is to increase the efficiency of the biodegradation of polychlorinated biphenyls (PCBs) by the introduction of bacterial genes into the plant genome. For this purpose, we selected the bphC gene encoding 2,3-dihydroxybiphenyl-1,2-dioxygenase from Pseudomonas testosteroni B-356 to be cloned into tobacco plants. The dihydroxybiphenyldioxygenase enzyme is the third enzyme in the biphenyl degradation pathway, and its unique function is the cleavage of biphenyl. Three different constructs were designed and prepared in E. coli: the bphC gene being fused with the beta-glucuronidase (GUS) gene, with the luciferase (LUC) gene, and with histidine tail in three separate plant cloning vectors. The GUS and LUC genes were chosen because they can be used as markers for the easy detection of transgenic plants, while histidine tail better enables the isolation of protein expressed in plant tissue. The prepared vectors were then introduced into cells of Agrobacterium tumefaciens. The transient expression of the prepared genes was first studied in cells of Nicotiana tabacum. Once this ability had been established, model tobacco plants were transformed by agrobacterial infection with the bphC/GUS, bphC/LUC, and bphC/His genes. The transformed regenerants were selected on media using a selective antibiotic, and the presence of transgenes and mRNA was determined by PCR and RT-PCR. The expression of the fused proteins BphC/GUS and BphC/LUC was confirmed histochemically by analysis of the expression of their detection markers. Western blot analysis was performed to detect the presence of the BphC/His protein immunochemically using a mouse anti-His antibody. Growth and viability of transgenic plants in the presence of PCBs was compared with control plants.


Subject(s)
Dioxygenases/genetics , Dioxygenases/metabolism , Nicotiana/metabolism , Plants, Genetically Modified/metabolism , Polychlorinated Biphenyls/metabolism , Agrobacterium tumefaciens/genetics , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Biodegradation, Environmental , Cloning, Molecular , Comamonas testosteroni/enzymology , Comamonas testosteroni/genetics , Gene Expression , Genes, Reporter , Genetic Vectors , Glucuronidase/genetics , Glucuronidase/metabolism , Luciferases/genetics , Luciferases/metabolism , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/genetics , Plants, Genetically Modified/growth & development , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Nicotiana/enzymology , Nicotiana/genetics , Nicotiana/growth & development
13.
Physiol Behav ; 95(1-2): 187-93, 2008 Sep 03.
Article in English | MEDLINE | ID: mdl-18586285

ABSTRACT

We studied levels of fecal glucocorticoid metabolites (GCM) in a social rodent - Egyptian spiny mouse. As breeding adults are socially dominant over subadults, and adolescent males are driven away by the dominant males, we addressed the question whether animals within extended families are stressed differently depending upon their social category. In addition, we evaluated whether there are differences between non-commensal (outdoor) and commensal (adapted to human settlements) populations. Concentrations of fecal GCM were assessed from samples collected in a special cage that allowed continuous individual sampling of undisturbed mice housed as a semi-natural social unit. First we performed an ACTH challenge test to validate two enzyme immunoassays (EIA): a 5alpha-pregnane-3beta,11beta,21-triol-20-one EIA and an 11-oxoetiocholanolone EIA to measure a group of fecal GCM in this species. Next we monitored concentrations of fecal GCM in 68 individuals belonging to 10 family groups and two populations. Commensal spiny mice showed higher fecal GCM levels than non-commensal ones. No effect of age (i.e., social dominance) and only a small effect of sex (in the commensal population only, with males exhibiting lower values) on fecal GCM levels were found. On the other hand, considerable variations in measured fecal GCM between family groups were revealed, indicating that the social settings of the particular group play an important role.


Subject(s)
Aging/metabolism , Corticosterone/metabolism , Environment , Feces/chemistry , Sex Characteristics , Adrenocorticotropic Hormone/administration & dosage , Analysis of Variance , Animals , Behavior, Animal , Etiocholanolone/analogs & derivatives , Etiocholanolone/metabolism , Female , Immunoenzyme Techniques/methods , Male , Murinae , Pregnanetriol/analogs & derivatives , Pregnanetriol/metabolism , Social Behavior , Time Factors
14.
Gen Physiol Biophys ; 27(1): 45-54, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18436983

ABSTRACT

Voltage-sensitive dyes (VSDs) are used to record transient potential changes in various cardiac preparations. In our laboratory, action potentials have been recorded by optical probe using di-4-ANEPPS. In this study, the effects of two different ways of staining were compared in guinea pig and rabbit isolated hearts perfused according to Langendorff: staining either by coronary perfusion with low dye concentration or with concentrated dye as a bolus into the aorta. Staining with low dye concentration lead to its better persistence in the tissue. Electrogram and coronary flow were monitored continuously. During the staining and washout of the dye, prominent electrophysiological changes occurred such as a decrease in spontaneous heart rate, partial atrioventricular block and changes of ST-T segment, accompanied by a decrease in mean coronary flow. No production of hydroxyl radicals was found by HPLC which excluded significant ischemic damage of the myocardium. Good viability of the stained preparation was supported by unchanged electron microscopy. Since in rabbit hearts the VSD-induced arrhythmogenesis was less pronounced, we conclude that the rabbit myocardium is more resistant to the changes triggered by VSD application. It may be due to different properties of the membrane potassium channels in the cardiomyocytes of these two species.


Subject(s)
Fluorescent Dyes/toxicity , Myocardium/metabolism , Pyridinium Compounds/toxicity , Action Potentials , Animals , Arrhythmias, Cardiac/chemically induced , Arrhythmias, Cardiac/physiopathology , Electrophysiology , Guinea Pigs , Heart/drug effects , Heart/physiopathology , In Vitro Techniques , Microscopy, Electron, Transmission , Myocardium/ultrastructure , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/metabolism , Perfusion , Potassium Channels/metabolism , Rabbits , Staining and Labeling
15.
Physiol Res ; 67(Suppl 4): S561-S576, 2018 12 31.
Article in English | MEDLINE | ID: mdl-30607964

ABSTRACT

More than four decades passed since sigma receptors were first mentioned. Since then, existence of at least two receptor subtypes and their tissue distributions have been proposed. Nowadays, it is clear, that sigma receptors are unique ubiquitous proteins with pluripotent function, which can interact with so many different classes of proteins. As the endoplasmic resident proteins, they work as molecular chaperones - accompany various proteins during their folding, ensure trafficking of the maturated proteins between cellular organelles and regulate their functions. In the heart, sigma receptor type 1 is more dominant. Cardiac sigma 1 receptors regulate response to endoplasmic reticulum stress, modulates calcium signaling in cardiomyocyte and can affect function of voltage-gated ion channels. They contributed in pathophysiology of cardiac hypertrophy, heart failure and many other cardiovascular disorders. Therefore, sigma receptors are potential novel targets for specific treatment of cardiovascular diseases.


Subject(s)
Cardiovascular Agents/administration & dosage , Cardiovascular Diseases/drug therapy , Cardiovascular Diseases/metabolism , Myocardium/metabolism , Myocytes, Cardiac/metabolism , Receptors, sigma/metabolism , Animals , Cardiovascular Agents/metabolism , Cardiovascular Diseases/pathology , Humans , Myocardium/pathology , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/pathology , Receptors, sigma/antagonists & inhibitors , Sigma-1 Receptor
16.
Physiol Res ; 67(4): 623-636, 2018 08 16.
Article in English | MEDLINE | ID: mdl-29750874

ABSTRACT

Several members of the TGF-beta family are known to effectively regulate the fate of hematopoietic progenitor cells in a complex and context-dependent manner. Growth differentiation factor-15 (GDF15) is a divergent member of the TGF-beta family. This stress-induced cytokine has been proposed to possess immunomodulatory functions and its high expression is often associated with progression of a variety of pathological conditions. GDF15 is also induced by chemotherapy and irradiation. Very few fundamental studies have been published regarding the effect of GDF15 in hematopoiesis. In this study, we analyzed the hematological status of untreated and gamma-irradiated mice deficient for GDF15 as a result of genetic knock-out (KO), in order to clarify the regulatory role of GDF15 in hematopoiesis. Significant differences between GDF15 KO mice and their pertinent WT controls were found in the parameters of blood monocyte numbers, blood platelet size, and distribution width, as well as in the values of bone marrow granulocyte/macrophage progenitor cells. Different tendencies of some hematological parameters in the GDF15 KO mice in normal conditions and those under exposure of the mice to ionizing radiation were registered. These findings are discussed in the context of the GDF15 gene function and its lack under conditions of radiation-induced damage.


Subject(s)
Gamma Rays/adverse effects , Growth Differentiation Factor 15/deficiency , Growth Differentiation Factor 15/radiation effects , Hematopoiesis/radiation effects , Animals , Bone Marrow Cells/metabolism , Bone Marrow Cells/radiation effects , Female , Hematopoiesis/physiology , Male , Mice , Mice, Inbred C57BL , Mice, Knockout
17.
Leukemia ; 32(4): 874-881, 2018 04.
Article in English | MEDLINE | ID: mdl-29089646

ABSTRACT

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide towards the relevant classification panel (T-cell acute lymphoblastic leukemia (T-ALL), B-cell precursor (BCP)-ALL and/or acute myeloid leukemia (AML)) and final diagnosis. Now we built a reference database with 656 typical AL samples (145 T-ALL, 377 BCP-ALL, 134 AML), processed and analyzed via standardized protocols. Using principal component analysis (PCA)-based plots and automated classification algorithms for direct comparison of single-cells from individual patients against the database, another 783 cases were subsequently evaluated. Depending on the database-guided results, patients were categorized as: (i) typical T, B or Myeloid without or; (ii) with a transitional component to another lineage; (iii) atypical; or (iv) mixed-lineage. Using this automated algorithm, in 781/783 cases (99.7%) the right panel was selected, and data comparable to the final WHO-diagnosis was already provided in >93% of cases (85% T-ALL, 97% BCP-ALL, 95% AML and 87% mixed-phenotype AL patients), even without data on the full-characterization panels. Our results show that database-guided analysis facilitates standardized interpretation of ALOT results and allows accurate selection of the relevant classification panels, hence providing a solid basis for designing future WHO AL classifications.


Subject(s)
Leukemia, Myeloid, Acute/pathology , Acute Disease , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Immunophenotyping/methods , Infant , Infant, Newborn , Male , Middle Aged , Precursor Cell Lymphoblastic Leukemia-Lymphoma/pathology , Young Adult
18.
Gen Physiol Biophys ; 26(2): 110-7, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17660585

ABSTRACT

The aim of the present work was to study the effect of various stressors (hypoxia, cold, immobilization) on the gene expression of sigma receptors in the left ventricles of rat heart. We have clearly shown that gene expression of sigma receptors is upregulated by strong stress stimuli, such as immobilization and/or hypoxia. Nevertheless, cold as a milder stressor has no effect on sigma receptor's mRNA levels. Signalling cascade of sigma receptors is dependent on IP(3) receptors, since silencing of both, type 1 and 2 IP(3) receptors resulted in decreased mRNA levels of sigma receptors. Physiological relevance of sigma receptors in the heart is not clear yet. Nevertheless, based on the already published data we can assume that sigma receptors might participate in contractile responses in cardiomyocytes.


Subject(s)
Gene Expression Regulation , Myocytes, Cardiac/metabolism , Receptors, sigma/genetics , Stress, Physiological/genetics , Stress, Physiological/physiopathology , Age Factors , Animals , Cells, Cultured , Heart Ventricles/metabolism , Heart Ventricles/physiopathology , Hypothermia, Induced , Hypoxia , Immobilization , Inositol 1,4,5-Trisphosphate Receptors/metabolism , Male , Mice , Mice, Inbred Strains , Myocytes, Cardiac/pathology , RNA Interference , RNA, Messenger/analysis , Rats , Rats, Inbred SHR , Rats, Inbred WKY , Rats, Sprague-Dawley , Receptors, sigma/metabolism
19.
Ceska Gynekol ; 72(4): 299-304, 2007 Aug.
Article in Czech | MEDLINE | ID: mdl-17966613

ABSTRACT

OBJECTIVE: Lymphoedema is a severe postoperative complication after treatment of many malignancies. It is a pathological accumulation of extracellular water (ECW). Early diagnostic tool is needed. Multifrequency bioimpedance analysis (MFBIA) is a method for detection of changes in ECW. TYPE OF STUDY: Prospective study. SETTING: Dept. of Obstetrics and Gynaecology of the 2nd Medical Faculty, Charles University, Prague. METHODS: We measured a control group of 72 women and a group of 74 patients undergoing a breast cancer surgery during 18 month after the surgery by MFBIA and circumferency. Characteristics of the patients were recorded. The detection of lymphoedema was done using MFBIA, circumferency measurement and upon the symptoms of the patients. RESULTS: The average age in the controll and tested group was 40,1 and 58,9 years. The average size of the tumour, grade and positivity if oestrogen receptors was 15,1 mm, 2,04 and 43%. In 23 patients (21%) complete lymphadenectomy was performed, in 51 patients (79%) a detection of sentinel lymph node was performed. Lymphoedema was detected in 8 women (11%). In these patients MFBIA detected lymphoedema 9 month earlier in total than other methods. CONCLUSION: MFBIA is a low-cost and precise method for the detection od early stage postoperative lymphoedema. We recommend to incorporate MFBIA into standard dispensatory plan of every patient combined with circumferency measurement.


Subject(s)
Arm , Breast Neoplasms/surgery , Lymphedema/diagnosis , Mastectomy, Modified Radical/adverse effects , Mastectomy, Segmental/adverse effects , Adult , Aged , Anthropometry , Body Composition , Electric Impedance , Female , Humans , Lymphedema/etiology , Middle Aged
20.
Physiol Res ; 66(Suppl 4): S553-S560, 2017 12 30.
Article in English | MEDLINE | ID: mdl-29355384

ABSTRACT

Anthracyclines represent one of the important classes of anti-cancer drugs; however, their major disadvantage is their profound cardiovascular toxicity. This study aimed to evaluate influence of anthracyclines on cardiovascular stiffness parameters estimated from pulse wave (PW). PW was measured in 59 cancer survivors treated with anthracyclines in childhood and in 248 healthy age-matched controls. Both patients and controls were divided into three age groups (13 - 15, 16 - 18 and 19 - 24 years). Central PW augmentation index (C-AI75) and augmentation pressure (C-AP75), both normalized to heart rate 75 bpm, were calculated as parameters of arterial wall stiffness. Central Buckberg sub-endocardial viability ratio (SEVR) was calculated as a parameter of diastolic function. Patients and controls were compared in each age group. C-AI75 and C-AP75 were significantly increased in patients in age groups 16 - 18 and 19 - 24 years. SEVR was decreased in patients in the oldest age group. Our results suggest that although toxic influence of anthracyclines to arterial wall and heart are developing during childhood and puberty, they can be detected rather in the adulthood. These changes are yet subclinical; however, their presence indicates potentially increased cardiovascular risk in childhood cancer survivors treated with anthracyclines during childhood.


Subject(s)
Anthracyclines/adverse effects , Antibiotics, Antineoplastic/adverse effects , Cancer Survivors , Pulse Wave Analysis/methods , Vascular Diseases/physiopathology , Vascular Stiffness/physiology , Adolescent , Blood Pressure/drug effects , Blood Pressure/physiology , Female , Heart Rate/drug effects , Heart Rate/physiology , Humans , Male , Treatment Outcome , Vascular Diseases/chemically induced , Vascular Diseases/diagnosis , Young Adult
SELECTION OF CITATIONS
SEARCH DETAIL