High-Throughput DNA sequencing of ancient wood.

Reconstructing the colonization and demographic dynamics that gave rise to extant forests is essential to forecasts of forest responses to environmental changes. Classical approaches to map how population of trees changed through space and time largely rely on pollen distribution patterns, with only a limited number of studies exploiting DNA molecules preserved in wooden tree archaeological and subfossil remains. Here, we advance such analyses by applying high-throughput (HTS) DNA sequencing to wood archaeological and subfossil material for the first time, using a comprehensive sample of 167 European white oak waterlogged remains spanning a large temporal (from 550 to 9,800 years) and geographical range across Europe. The successful characterization of the endogenous DNA and exogenous microbial DNA of 140 (~83%) samples helped the identification of environmental conditions favouring long-term DNA preservation in wood remains, and started to unveil the first trends in the DNA decay process in wood material. Additionally, the maternally inherited chloroplast haplotypes of 21 samples from three periods of forest human-induced use (Neolithic, Bronze Age and Middle Ages) were found to be consistent with those of modern populations growing in the same geographic areas. Our work paves the way for further studies aiming at using ancient DNA preserved in wood to reconstruct the micro-evolutionary response of trees to climate change and human forest management.

Punk's not dead. Fungi for tinder at the Neolithic site of La Draga (NE Iberia).

This paper presents the study of the fungi remains preserved in the waterlogged deposits of the Neolithic site of La Draga. These resources had the potential of being used as food and medicine, but also as tinder. Fire was without a doubt one of the most important resources for past people. It was used for lighting, heating, processing food and other materials, cooking and protection, and also possessed social and ritual significance. Hearths are one of the most common features at archaeological sites, but very often little attention is paid to the question of how these fires were lit, and they are seldom reflected in the archaeological record. In order to produce fire by percussion, an intermediate material is required between the sparks and the fuel. Fruiting bodies of fungi are a potential form of tinder, but are less inclined to be well-preserved than other materials. This paper presents the fungal fruiting bodies found at the Neolithic site of La Draga and discusses the meaning of their presence within the archaeological context of the site and European Prehistory.