A systematic review on garbage enzymes and their applications in environmental processes.

Understanding the new insight on conversion of organic waste into value-added products can improve the environmental activities driven by microorganisms and return the nutrients to environment and earth. Here, we comprehensively review the available knowledge on application of garbage enzyme (GE) for different environmental activities including waste activated sludge, composting process, landfill leachate treatment, soil remediation and wastewater treatment with special focus on their efficiency. To identify peer-reviewed studies published in English-language journals, a comprehensive search was performed across multiple electronic databases including Scopus, Web of Science, Pubmed, and Embase. The search was conducted systematically using relevant keywords. The eligible studies were analyzed to extract data and information pertaining to components of GE, fermentation process operational parameters, type of hydrolytic enzymes and improved environmental performance. The findings derived from this current review demonstrated that GE produced from the fruit and vegetable peels, molasses or brown sugar (carbon source), and water within fermentation process contain different hydrolytic enzymes in order to facilitate the organic waste degradation. Therefore, GE can be considered as a promising and efficient pathway in order to improve the environmental activities depended on microorganism including, composting, wastewater and leachate treatment and bioremediation process.

A comprehensive perspective on potentially hazardous elements (PHEs) bound with particulate matters in ambient air of landfill sites: a systematic review and probabilistic risk assessment.

Air pollution from Potentially Hazardous Elements bound with particulate matter (PHEs bound PM) in landfill air is a significant concern for human health. To date, no comprehensive research has focused on the health risks of PHEs bound to PM in landfill air. This systematic review aimed to examine PHEs, including Lead (Pb), Cadmium (Cd), Chromium (Cr), Arsenic (As), Nickel (Ni), and Zinc (Zn) bound with PM in landfill air and assess the health risk for workers and waste management personnel. The systematically search was made in different electronic databases. After the screening, 18 most relevant studied focused on PHEs bound PM in landfill air were selected. The data extraction analysis indicated that mean concentrations of Pb, Cd, Cr, As, Ni, and Zn in landfill air were 0.3037, 0.0941, 0.4093, 0.0221, 0.2768, and 0.7622 µg/m3, respectively. Except for Pb, the concentrations of other PHEs bound to PM exceeded USEPA air quality standards. In addition, Non-carcinogenic risks from Cd, Cr, and Ni exposure exceeded permissible limits (HQ > 1), while As had an HQ value of 0.401. Carcinogenic risks from As, Cd, Ni, and Pb exposure were 1.31 × 10-5, 5.10 × 10-4, 3.51 × 10-5, and 2.03 × 10-6, respectively. Notably, the lifetime carcinogenic risk for workers exposed to Cr in polluted air (6.17 × 10-3) exceeded permissible limits (10-4 to 10-6). In conclusion, given the high carcinogenic and non-carcinogenic risks of some PHEs bound PM in landfills, it is necessary to conduct more research on the health effects of interaction with these PHEs bound PM on communities and the environment in different countries. Also, it is necessary to evaluate the role of different landfill operational activities on atmospheric dispersion of PHEs bound PM) in landfill air.

Upgrading the biogas production from raw landfill leachate using O3/H2O2 pretreatment process: Modeling, optimization and anaerobic digestion performance.

Here, a combined pretreatment oxidation process (O3/H2O2) was investigated to enhance the biodegradability of raw landfill leachate (RLL) and biomethane potential (BMP) in anaerobic reactors. The central composite design (CCD) and response surface methodology (RSM) were employed to optimize the operational parameters influencing on RLL bioavailability in O3/H2O2 process: pH, Oxygen Flow rate, Reaction Time, and H2O2 concentration. The findings revealed that the O3/H2O2 increased biodegradability index (BOD5/COD) of RLL from 0.41 to 0.68 under optimized condition (pH=8, Oxygen flow= 0.25 L.min-1, Reaction Time= 25 min, H2O2 concentration= 2.5 g.L-1). Furthermore, the effects of O3/H2O2 process on BMP of RLL were surveyed under mesophilic anaerobic reactors (Temperature: 37 ± 1 °C) in viewpoints of operational performance and methane yield in a batch mode for incubation period of 24 days. The results showed that O3/H2O2 process simultaneously improve the BMP by 2.99 times higher in a shorter lag-phase period (5 days) compared with control. The pretreatment O3/H2O2 and mesophilic anaerobic digestion process revealed a feasible and efficient method for enhance BMP of RLL.

Bio-electrical stimulation process on degradation of Phenanthrene from aqueous solution using a novel anode modified with carbon cloth: Operational performance, microbial activity and energy.

Phenanthrene as the hazardous PAHs-component are extensively detected in industrial wastewater. However, the impacts of bioelectrostimulation process on Phenanthrene degradation in aerobic reactors remained unclear. Here, a novel bioelectrostimulation process equipped with carbon cloth as electrodes was developed to investigate the removal efficiency of Phenanthrene and ATPase enzyme activity in the synthetic wastewater. The results obtained from the present study indicated that a complete Phenanthrene degradation (100%) can be achieved using microbial electrostimulation systems steel mesh coated with carbon cloth (MES-CC) as anode under optimal operational conditions (electrical current: 4 mA, HA concentration: 15 mg L-1) within 18 h. The conductive carbon cloth provides a biofilm carrier to easily transfer the electrons between electrodes and microbial communities. In addition, the highest ATPase enzyme activity (5176 U) was observed when the aerobic MES-CC reactors were operated with electrical current 4 mA. Furthermore, the COD removal efficiency in MES-CC increased from 49% to 96% when the C: N ratio decreased from 20 to 5. The highest value of Vmax in MES-CC for suspended and attached growth were determined to be 2.87 and 0.54 g COD g-1 biomass. Overall, the results demonstrated that MES equipped with carbon cloth and continuous electrical current mode has good potential for efficient Phenanthrene wastewater treatment.

The effects of different pretreatment technologies on microbial community in anaerobic digestion process: A systematic review.

Here we comprehensively review the available knowledge on effects of different pretreatment technologies on microbial population and microbial dynamics in anaerobic digestion (AD) fed with different substrates and different operational parameters. To identify peer-reviewed studies published in English-language journals, a comprehensive search was performed across multiple electronic databases. The eligible studies were analyzed to extract data and information pertaining to the configuration of anaerobic reactors, operational parameters, and various pretreatment processes such as chemical, biological, enzymatic, thermal, microaerobic, and ultrasonic. The findings derived from this current review demonstrated that different chemical, biological, and physical pretreatment technologies improve the biomethane potential (BMP) and potentially affect the dominant bacteria and archaea. Moreover, although hydrogenotrophic methanogenesis are more observed due to resistance to extreme conditions, methane production follows both aceticlastic and hydrogenotrophic pathways in AD assisted with different pretreatment process. Firmicutes and Bacteroidetes phyla of bacteria were the dominant hydrolytic bacteria due to synergetic effects of different pretreatment process on solubilization and bioavailability of recalcitrant substrates. In summary, a holistic understanding on bacteria and archaea communities, along with the mechanisms of the dominant microorganisms leads to enhanced stability and overall performance of anaerobic digestion (AD) processes. Supplementary Information: The online version contains supplementary material available at 10.1007/s40201-024-00917-x.

Biological feasibility of discharge a local WTTP sludge to sewer network and centralized WWTP; a case study: Tehran, Iran.

Over the recent years, ever-increasing population growth and higher wastewater production has been a challenge for decentralized wastewater treatment plants (WWTPs). In addition, sludge treatment due to high cost for equipment and place make authorities to find a sustainable approach in both of economical and technical perspectives. One of the proposed solutions is transferring the sludge produced from decentralized WWTP to centralized WWTP. However, the appropriate proportional ratio of raw sludge to raw sewage is a challenge, otherwise, it make anaerobic conditions and sewage rotting along the sewer network based on permissible limit of dihydrogen sulfide (H2S) gas (5 ppm). In the present study, seven reactors with different ratios of sludge to raw sewage (0, 15, 20, 25, 50, 75, 100) were used to stimulate the feasibility of transferring Shahrake Gharb WWTP sludge along the wastewater transfer pipe to the centralized sewage treatment south Tehran WWTP plant in Tehran, Iran. The septic situation and H2S emission of different reactors within 7 h (Time to reach the compound in the south treatment plant) was analyzed by gas meter. The results indicated that the optimum ratio of sludge to raw sewage was 15% without H2S production during 7 h. In addition, due to the high volume of sludge produced by the Shahrake Gharb WWTP, the optimal ratio of lime to total solids (TS) in sludge (gr/gr) (0.6) increased the sludge loading rate from 15 to 30% without any H2S emission during the stimulation study period. Therefore, the lime stabilization and transfer of sludge from a decentralized WWTP to a centralized WWTP is a feasible way to manage the sludge and enhance the treatment capacity in local WWTP.

Three-layer business model canvas (TLBMC) as a recycling support tool to achieve sustainable development goals in waste management systems.

Traditional strategies for waste management in developing countries face high-cost problems, severe pollution, limited viable data, lack of global coverage, inefficiency, and slowness. The rapid expansion of the Internet have made new opportunities in waste management, especially waste collection. Online waste management in developing countries can create a revolution and help the government and society to achieve the goals of sustainable development. In this study, a modified three-layer business model canvas (TLBMC) was used to describe the duties of each actors involved in waste management and interactions between different elements in order to show the existing capacities in view point of economic, social, and environmental dimensions. In this online marketplace, the flow of information, the flow of materials and the flow of money in any company or entity or person active in the field of waste, are effectively connected to each other. Supply and demand information is provided in the created platform, and various waste actors are connected to look for options to trade. After agreement between the customer and the buyer, the information is delivered from the website or platform created by the supplier to the applicant. This network can have its usual structural features and create appropriate mechanisms for the continuation of its business operation and the realization of sustainable development of waste management, especially the recycling industry.

Hydraulic feasibility of discharging sludge from a local WWTP to a centralized WWTP through sewage network lines.

Over the recent years, due to the increase in the population covered by local wastewater treatment plants (WWTP), upgrading the existing treatment plants needs special attention more than ever. One of the suggested solutions removal the existing sludge treatment units in local WWTP and transfer the sludge to a centralized WWTP. The present study was developed to investigate the hydraulic feasibility of sludge transfer from Shahrak-e-GharbWWTP as a local treatment plant to South Tehran sewage treatment plant in Tehran. To this end, at first, a map containing descriptive information and hydraulic characteristics related to the sewage transmission network between local and centralized WWTP was collected from the sewage company. This information was used to calculate the maximum capacity, current flow and draw the hydraulic profile of the sewage transmission line. Then, the transmission line profile was drawn using Manning's hydraulic model and SewerCAD v10.01 software. According to the obtained results, areas 1 and 3 with sewage line diameters of 1000 and 1400 mm did not have any special problem in terms of entering the sludge. Hydraulic analyzes showed that some lines in areas 2, 4, 5, and 6 suffered setbacks and crises after the increase of sludge, which require auxiliary lines. The results indicated that more than 85% of the sewage network lines have the ability to transfer the excess sludge of the local treatment plant, however, in 36 lines, mostly located in zones 2 and 6, with a diameter of 1200 and 2000 mm, there is a crisis of sewage filling and backflow. Therefore, in order to reduce hydraulic stresses in these lines, it was suggested to build an auxiliary line (bypass ring at the beginning and end of these lines).

A comparative study on polyaluminum chloride (PACl) and Moringa oleifera (MO) chemically enhanced primary treatment (CEPT) in enhanced biogas production: anaerobic digestion performance and the Gompertz model.

A comparative study was performed to estimate biogas production from sludge produced by organic and inorganic chemically enhanced primary treatments (CEPTs). To this end, the effects of two coagulants, polyaluminum chloride (PACl) and Moringa oleifera (MO), on CEPT and biogas production in anaerobic digestion were surveyed within an incubation period of 24 days. The optimal dosage and pH of PACl and MO were optimized in terms of sCOD, TSS and VS parameters in the CEPT process. Next, the digestion performance of anaerobic digestion reactors fed with sludge obtained from PACl and MO coagulants at a batch mesophilic reactor (37 ± 1 °C) was surveyed from the biogas production, volatile solid reduction (VSR) and Gompertz model. At the optimal conditions (pH = 7 and dosage = 5 mg L-1), the removal efficiency of COD, TSS and VS in CEPT assisted with PACL was 63, 81 and 56%, respectively. Moreover, CEPT assisted with MO led to the removal efficiency of COD, TSS and VS until 55, 68 and 25%, respectively. The highest methane yield (0.598 L gVS removed-1) was obtained in an anaerobic digestion reactor with sludge from the MO coagulant. The anaerobic digestion of CEPT sludge instead of primary sludge resulted in higher sCOD removal efficiency, and 43-50% of sCOD was observed compared with the removal of 32% for the primary sludge. Furthermore, the high coefficient of determination (R2) demonstrated the trustworthy predictive precision of the modified Gompertz model with actual data. The combination of CEPT and anaerobic digestion, especially using natural coagulants, provides a cost-effective and practical way to increase BMP from primary sludge.

Is SARS-CoV-2 a concern in the largest wastewater treatment plant in middle east?

The surveillance of wastewater treatment plant (WWTP) as the end point of SARS-CoV-2 shed from infected people arise a speculation on transmission of this virus of concern from WWTP in epidemic period. To this end, the present study was developed to comprehensively investigate the presence of SARS-CoV-2 in raw wastewater, effluent and air inhaled by workers and employee in the largest WWTP in Tehran for one-year study period. The monthly raw wastewater, effluent and air samples of WWTP were taken and the SARS-CoV-2 RNA were detected using QIAamp Viral RNA Mini Kit and real-time RT-PCR assay. According to results, the speculation on the presence of SARS-CoV-2 was proved in WWTP by detection this virus in raw wastewater. However, no SARS-CoV-2 was found in both effluent and air of WWTP; this presents the low or no infection for workers and employee in WWTP. Furthermore, further research are needed for detection the SARS-CoV-2 in solid and biomass produced from WWTP processes due to flaks formation, followed by sedimentation in order to better understand the wastewater-based epidemiology and preventive measurement for other epidemics probably encountered in the future.

Assessment of rotavirus and norovirus emitted from water spray park: QMRA, diseases burden and sensitivity analysis.

A quantitative model on exposure to pathogenic viruses in air of recreational area and their corresponding health effects is necessary to provide mitigation actions in content of emergency response plans (ERP). Here, the health risk associated with exposure to two pathogenic viruses of concern: Rotavirus (RoV) and Norovirus (NoV) in air of water spray park were estimated using a quantitative microbial risk assessment (QMRA) model. To this end, real-time Reverse Transcriptase polymerase chain reaction (real-time RT-PCR) was employed to measure the concentration levels of RoV and NoV over a twelve-month period. The probability of infection, illness and diseases burden of gastrointestinal illness (GI) caused by RoV and NoV for both workers and visitors were estimated using QMRA and Monto-Carlo simulation technique. The annual mean concentration for RoV and NoV in sampling air of water spray park were 20and 1754, respectively. The %95 confidence interval (CI) calculated annual DALY indicator for RoV (Workers: 2.62 × 10-4-2.62 × 10-1, Visitors: 1.50 × 10-5-2.42 × 10-1) and NoV (Workers: 5.54 × 10-3-2.53 × 10-1; Visitors: 5.18 × 10-4-2.54 × 10-1) were significantly higher the recommended values by WHO and US EPA (10-6-10-4 DALY pppy). According to sensitivity analysis, exposure dose and disease burden per case (DBPC) were found as the most influencing factors on disease burden as a consequences of exposure to RoV and NoV, respectively. The comprehensive information on DALY and QMRA can aid authorities involved in risk assessment and recreational actions to adopt proper approach and mitigation actions to minimize the health risk.

The potential osteoporosis due to exposure to particulate matter in ambient air: Mechanisms and preventive methods.

Air pollution and health consequences associated with exposure to air pollutants, such as particulate matter, are of serious concerns in societies. Over the recent years, numerous studies have investigated the relation of many diseases with air pollutants. This review used a search strategy to provide the comprehensive information on the relationship between particle matters and osteoporosis. To this end, three search databases were used to find the articles focused on particle matters and osteoporosis. After the screening process, 13 articles related to the purpose of the study were selected and the relevant data were extracted. The results indicated that osteoporosis is significantly associated with PM10. However, this association with PM2.5 remains unclear. In addition, particle materials indirectly lead to the osteoporosis and bone fractures as a consequence of reduced UV-B, reduced adsorption of vitamin D. Furthermore, they can lead to other diseases by use of drugs with adverse effects on bone health, and creating conditions that may increase the risk of falling in the elderly. This review shows that although more accurate research is needed to determine the mechanism and risk of exposure to particulate matter in the air on bone health, the negative effects of this pollutant on bone mineral density (BMD) are evident.Implications: PM is usually classified by its size or aerodynamic diameter; PM10 denotes particles < 10 µm in diameter; PM2.5 particles are <2.5 µm in diameter. Many epidemiological studies have shown that short-term exposure to PM might reduce lung function. However, short-term effects might be reversible, and the main concern is attributed to long-term exposure. A major public health concern that may be affected by numerous metabolic and even environmental risk factors is osteoporosis. The purpose of this systematic review was to investigate the role of PM in the occurrence or exacerbation of osteoporosis in citizens.

Urban air pollution control policies and strategies: a systematic review.

A wide range of policies, strategies, and interventions have been implemented to improve air quality all over the world. This systematic review comprehensively appraises the policies and strategies on air pollutants controls enacted in different countries, worldwide. Three databases, Web of Science, PubMed and Scopus, were used for the search. After screening, a total of 114 eligible manuscripts were selected from 2219 documents for further analysis. Selected articles were divided into two categories: (1) articles focusing on introducing the policies and strategies enacted for controlling air pollution in different countries, and (2) articles which focused on different policies and strategies to control one or more specific pollutants. In the former one, urban air pollution control strategies and policies were divided into four categories, namely, general strategies and policies, transportation, energy, and industry. In case of latter category, policies and strategies focused on controlling six pollutants (PM, SO2, NO2, VOCS, O3 and photochemical smog). The results indicated that, the most common policies and strategies enacted in most countries are pertinent to the transportation sector. Changing energy sources, in particular elimination or limited use of solid fuels, was reported as an effective action by governments to reduce air pollution. Overall, most policies enacted by governments can be divided into three general categories: (a) incentive policies such as implementing a free public transportation program to use fewer private cars, (b) supportive policies such as paying subsidies to change household fuels, and (c) punitive policies such as collecting tolls for cars to enter the congestion charging areas. Depending on the circumstances, these policies are implemented alone or jointly. In addition to the acceptance of international agreements to reduce air pollution by governments, greater use of renewable energy, clean fuels, and low-pollution or no-pollution vehicles such as electric vehicles play an important role in reducing air pollution.

Assessment of co-composting of sewage sludge, woodchips, and sawdust: feedstock quality and design and compilation of computational model.

Composting process of sewage sludge requires a preprocessing step in order to prepare the appropriate mixture of dewatered sludge (Xs) with amendment (Xa), bulking agent (Xb), and/or recycled materials (Xr). This research aimed to develop a novel mathematical model for finding an optimal mixture ratio of dewatered sludge with the aforementioned influencing elements on co-composting process. Seven feasible scenarios were presented and the best one was selected in viewpoint of technical and economic perspectives. The optimum mixture was prepared and its quality was evaluated in the terms of physical, chemical, and microbial characteristics. The optimum mixture was loaded in an aerated static pile composting reactor in order to evaluate the quality of the final compost product. If the test results were not in compliance with the USEPA standards, the model was iteratively modified to fulfill the desired objective. The model was validated using the experimental results. The mixture of Xs:Xa:Xb:Xr with a weight ratio 7.4:1.0:1.4:2.3 allowed optimal moisture content (59.8 ± 0.5%), organic matter (80.0 ± 2.6%), dry matter (40.2 ± 0.6%), C/N ratio (28.0 ± 1.6), and free air space (> 30%) across the composting pile. The final product of compost met the heavy metal and microbial requirements for land application. It can be concluded this mathematical model is a promising method for selecting the optimal amount and type of materials for preparing the initial mixture of co-composting process.

Perspectives on microbial community in anaerobic digestion with emphasis on environmental parameters: A systematic review.

This paper review is aiming to comprehensively identify and appraise the current available knowledge on microbial composition and microbial dynamics in anaerobic digestion with focus on the interconnections between operational parameters and microbial community. We systematically searched Scopus, Web of Science, pubmed and Embase (up to August 2019) with relative keywords to identify English-language studies published in peer-reviewed journals. The data and information on anaerobic reactor configurations, operational parameters such as pretreatment methods, temperature, trace elements, ammonia, organic loading rate, and feedstock composition and their association with the microbial community and microbial dynamics were extracted from eligible articles. Of 306 potential articles, 112 studies met the present review objectives and inclusion criteria. The results indicated that both aceticlastic and hydrogenotrophic methanogenesis are dominant in anaerobic digesters and their relative composition is depending on environmental conditions. However, hydrogenotrophic methanogens are more often observed in extreme conditions due to their higher robustness compared to aceticlastic methangoens. Firmicutes and Bacteroidetes phyla are most common fermentative bacteria of the acidogenic phase. These bacteria secrete lytic enzymes to degrade organic matters and are able to survive in extreme conditions and environments due to their spores. In addition, among archaea Methanosaeta, Methanobacterium, and Methanosarcinaceae are found at high relative abundance in anaerobic digesters operated with different operational parameters. Overall, understanding the shifts in microbial composition and diversity as results of operational parameters variation in anaerobic digestion process would improve the stability and process performance.

The influence of combined low-strength ultrasonics and micro-aerobic pretreatment process on methane generation and sludge digestion: Lipase enzyme, microbial activation, and energy yield.

Low-frequency ultrasonics is a potential technology to reduce the hydrolysis phase period in anaerobic digestion process. In this study, theinfluence of combined low frequency ultrasonics and micro-aerobic (MA) pretreatment on sewage sludge solubilization, enzyme activity and anaerobic digestion were assessed. Initially, the effect of ultrasonic density (0.012, 0.014, 0.016, 0.018, 0.1, 0.12 and 0.14 W/mL) and irradiation time (1, 3, 5, 8, 9, 10 and 12 min) of 20 kHz frequency waves were investigated. Accordingly, the effect of micro-aerobic pretreatment (Air flow rate (AFR) = 0.1, 0.2, 0.3 and 0.5 VVM) within 20, 30, 40.48 and 60 h were examined. In addition, the effect of combined pretreatment on COD solubilization, lipase enzyme activation, ATP, percentage of live bacteria and methane gas production during the anaerobic process were examined. The results showed that the highest lipase activity (14.9 Umol/mL) was obtained under the effect of ultrasonic density of 0.1 W/ml within 9 min. The highest solubilization (65%) was observed under optimal micro-aerobic conditions: AFR = 0.2 (VVM) and micro-aerobic time: 40 h. Combined ultrasonic and micro-aerobic (US + MA) pretreatment increases the solubilization (70%), microbial activity (2080%) and lipase enzymatic activity (129%) compared to individual pretreatment. The Biogas production during anaerobic digestion pretreated with combined methods increased by 193% compared to the control, while the elevated values of biogas production in reactors pretreated by ultrasonic and micro-aerobic pretreatment alone were observed to be 101% and 165%, respectively. The net energy in reactor with the combined pre-treatment methods was calculated to be 1.26 kWh, while this value for control, pretreated ultrasonic and micro-aerobic reactors were obtained to be 0.56, 0.67 and 1.2 kWh, respectively.

Bioremediation of diesel and gasoline-contaminated soil by co-vermicomposting amended with activated sludge: Diesel and gasoline degradation and kinetics.

Present study aims to examine the efficiency of co-vermicomposting amended with activated sludge and E. fetida earthworm for bioremediation of diesel and gasoline from contaminated soil. The diesel and gasoline removal efficiency and degradation rates coefficients were estimated with gas chromatography (GC) analysis and first-order kinetics. The removal of gasoline and diesel in different co-vermicomposting processes with and without E. fetida ranged between 65-100% and 24.94-63.93%, respectively within 90- day experiment. Removal of gasoline and diesel increased in soil with addition of earthworm (E. fetida); higher degradation rate coefficients (k) were observed for co-vermicomposting with earthworm compared with co-vermicomposting processes. The highest k (0.014) for diesel degradation was estimated for microcosm reactor 4 (R4), where high numbers of E. fetida accelerate the less biodegradable organic contaminant from the soil matrices. The reasonable survival rates of earthworms in exposure to high concentration of petroleum-derivatives contaminated soils indicated increased activity of ligninolytic diesel-degrading earthworms and microorganisms. Therefore, co-vermicomposting amended with activated sludge is suggested as feasible and promising technologies for bioremediation of high content of organic contaminants from the soil matrices.

Improvement of Co-Composting by a combined pretreatment Ozonation/Ultrasonic process in stabilization of raw activated sludge.

The enhancement of composting technology to stabilize sludge pretreated by ozonation and ultrasonic was tested for 35 days. Secondary sludge produced by biological process are characterized with endogenous residue and inert solid matter which inhibit fully degrade bacterial cell walls. The composting process was performed with sludge pretreated with ozonatian and ultrasonics and green waste in a ratio of 2:1. The composting characteristics was evaluated for different physico-chemical and microbiological parameters in five different reactors. A high degree of composting quality was achieved with respect to significant reduction in volatile solids (VS) (32%), total organic carbon (TOC) (35.0%), C/N ratio (23.74), total coliform (TC) (168) along with the substantial increase in availability of nutrients like N (1.2%) and P (8.77%). High removal efficiency of TC and Fecal Coliform (FC) were observed in composting results, where simultaneous ultrasonic and ozonation were considered as primary-stabilization process. Therefore, applying integrated ultrasonic/ozonation with composting system for sludge stabilization is potentially useful technology in sustainable land restoration practices to meet standards and produce soil conditioner.

Pattern of disinfectants use and their adverse effects on the consumers after COVID-19 outbreak.

BACKGROUND AND OBJECTIVE: The aim of this study was to investigate the pattern of disinfectants use within outbreak of COVID-19 and estimate their adverse effects on the consumer's health. METHODS: In this descriptive-analytical study, 1090 participants were entered into the study without age and gender limitations. A researcher-made electronic checklist containing 43 questions was applied to collect data. The checklist included three sections: demographic characteristics (8 questions), disinfection of body and non-living surfaces (21 questions), and adverse effects of disinfectants on health (15 questions). RESULTS: 87% of participants used the incorrect proportions of water and alcohol to make this disinfectant available at home. The percentage of people with wrong proportion of sodium hypochlorite was 74.2%. Approximately 42% of participants experienced at least one disorder on their hands, feet, eyes, respiratory or gastrointestinal systems after sequential uses of disinfectants. The most common disorders among the participants were found to be skin dryness (76.3%), obsession (42.2%), skin itching (41.2%), coughing (41.1%), and eyes irritation (39.5%). The mean frequency of hand washing and hand disinfecting were 15.28 and 10.74 times per a day, respectively, and the clean-up in case of surfaces was 2.99 times a day. The frequency of hand washing and disinfecting in women group (16.4 and 11.2 times a day) were higher than in men (14.0 and 10.3 times a day) group. In addition, these self-care actions in married people (15.6 and 11.0 times a day) were higher compared to those in single people (14.0 and 10.6 times a day). CONCLUSION: Being unaware of participants with instruction for preparation and use disinfectants may harm their health. Therefore, it is suggested that the authorities provide the necessary training program for public through official media.

Landfill site selection using a hybrid system of AHP-Fuzzy in GIS environment: A case study in Shiraz city, Iran.

Landfilling with simplicity and economic advantages is the most common element for waste management in both developed and developing countries. Landfill site selection in a proper way is an important municipal planning process which prevent environmental issues including water pollution imposed for insanitary landfills. The present research was developed to exhibit a simplified method of multi criteria decision making (MCDM) and Fuzzy memberships in GIS environment to ascertain best landfill sites for Shiraz county, located south of Iran. 15 most common sub-criteria, documented in literature and implicated by Iranian environmental protection organization (IEPO) including surface water, ground water, land use, distance to well, soil type, slope, protected area, fault in environmental group, residential area, road, airport, village, infrastructure, historical area, wind direction in socio-economical group were selected and the weight of each criterion was determined based on expert's knowledge with use of analytical hierarchy process (AHP). The results of the present research are as follows: •Distance to residential area and groundwaters with weight of 0.36 and 0.28 were recognized as the most important criteria for landfill site selection.•The six suitable areas for landfill in Shiraz county is 1.003% of total area equal to 8710 ha.•AHP and Fuzzy memberships has a great potential and ability for landfill site selection.