A cost-effective tool to standardize the scratch assay for cell migration.

BACKGROUND: The scratch assay is commonly used in cell biology to evaluate cell migration; however, it is not a standardized method; it produces highly variable gap dimensions. We design a printable device, comprising a single wounding tool and a guide, and compared the gap produced by our device and the traditional method. The deviceis printable in a standard 3D printer. Cells were seeded on a 24-well plate. After reaching full confluency, a gap was created using the traditional method (scratch assay with a pipette tip), a pipette tip and the guide of the device, or the single wounding tool and the guide. The gaps were observed for up to 48 h under a light microscope and analyzed. RESULTS: The results show that the traditional method produces irregular and not straight gaps, and had the worst cell migration rates compared to the other groups. The wounding tool produced scrape signs at the well surface. CONCLUSION: The guide and pipette tip delivered the best results for the scratch assay. SIGNIFICANCE: The use of the guide and the pipette tip for the scratch assay allows allows to perform reproducible cell migration experiments.

The Long Non-Coding RNA MALAT1 Modulates NR4A1 Expression through a Downstream Regulatory Element in Specific Cancer Cell Types.

Long non-coding RNAs (lncRNAs) have been shown to modulate gene expression and are involved in the initiation and progression of various cancer types. Despite the wealth of studies describing transcriptome changes upon lncRNA knockdown, there is limited information describing lncRNA-mediated effects on regulatory elements (REs) modulating gene expression. In this study, we investigated how the metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) lncRNA regulates primary target genes using time-resolved MALAT1 knockdown followed by parallel RNA-seq and ATAC-seq assays. The results revealed that MALAT1 primarily regulates specific protein-coding genes and a substantial decrease in the accessibility downstream of the NR4A1 gene that was associated with a decreased NR4A1 expression. Moreover, the presence of an NR4A1-downstream RE was demonstrated by CRISPR-i assays to define a functional MALAT1/NR4A1 axis. By analyzing TCGA data, we identified a positive correlation between NR4A1 expression and NR4A1-downstream RE accessibility in breast cancer but not in pancreatic cancer. Accordingly, this regulatory mechanism was experimentally validated in breast cancer cells (MCF7) but not in pancreatic duct epithelial carcinoma (PANC1) cells. Therefore, our results demonstrated that MALAT1 is involved in a molecular mechanism that fine-tunes NR4A1 expression by modulating the accessibility of a downstream RE in a cell type-specific manner.

Biological properties of stem cells from the apical papilla exposed to lipopolysaccharides: An in vitro study.

OBJECTIVE: The aim of this study was to analyze the effect of lipopolysaccharides (LPS) on the biological properties of stem cells from the apical papilla (SCAPs), such as viability, adhesion to dentin, odontoblast-like differentiation, mineralization, and release of immunomodulatory cytokines. DESIGN: SCAPs were isolated from immature teeth of three donors (10 to 15 years old) and cultured in mineralizing media with or without 1 µg/mL lipopolysaccharide (LPS). Cells were seeded and cultured under standardized conditions; viability was assessed by MTT assay on days 1, 3, 5, and 7; adhesion to dentin was analyzed using an environmental scanning electron microscope after 2 days; the expression of odontogenic and mineralization genes (DSPP, DMP-1, OCN, Col1A1) was evaluated through qPCR after 14 days, mineralization was evaluated with alizarin red staining after 21 days; and the release of immunomodulatory cytokines (IL-6 and IL-10) was measured by ELISA after 1 and 7 days. The Kruskal-Wallis test was performed to detect the effect of LPS on SCAPs, followed by the Dunn-Sidak test. RESULTS: LPS presence in the culture media affected SCAPs viability on day 5 and increased IL-6 secretion by day 7, however, SCAPs retained the adhesion to dentin and mineralization capacities, as well as the differentiation capacity into a mineralizing phenotype. CONCLUSION: In conclusion, within the limitations of this in vitro study, and under the inflammatory microenvironment simulated in this study, stem cells from the apical papilla were found with retained adhesion capacity to dentin, differentiation into a mineralizing phenotype, mineralization, and release of IL-10.

The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm.

Sperm capacitation, crucial for fertilization, occurs in the female reproductive tract and can be replicated in vitro using a medium rich in bicarbonate, calcium, and albumin. These components trigger the cAMP-PKA signaling cascade, proposed to promote hyperpolarization of the mouse sperm plasma membrane through activation of SLO3 K+ channel. Hyperpolarization is a hallmark of capacitation: proper membrane hyperpolarization renders higher in vitro fertilizing ability, while Slo3 KO mice are infertile. However, the precise regulation of SLO3 opening remains elusive. Our study challenges the involvement of PKA in this event and reveals the role of Na+/H+ exchangers. During capacitation, calcium increase through CatSper channels activates NHE1, while cAMP directly stimulates the sperm-specific NHE, collectively promoting the alkalinization threshold needed for SLO3 opening. Hyperpolarization then feeds back Na+/H+ activity. Our work is supported by pharmacology, and a plethora of KO mouse models, and proposes a novel pathway leading to hyperpolarization.

Comprehensive Development of a Cellulose Acetate and Soy Protein-Based Scaffold for Nerve Regeneration.

BACKGROUND: The elaboration of biocompatible nerve guide conduits (NGCs) has been studied in recent years as a treatment for total nerve rupture lesions (axonotmesis). Different natural polymers have been used in these studies, including cellulose associated with soy protein. The purpose of this report was to describe manufacturing NGCs suitable for nerve regeneration using the method of dip coating and evaporation of solvent with cellulose acetate (CA) functionalized with soy protein acid hydrolysate (SPAH). METHODS: The manufacturing method and bacterial control precautions for the CA/SPAH NGCs were described. The structure of the NGCs was analyzed under a scanning electron microscope (SEM); porosity was analyzed with a degassing method using a porosimeter. Schwann cell (SCL 4.1/F7) biocompatibility of cell-seeded nerve guide conduits was evaluated with the MTT assay. RESULTS: The method employed allowed an easy elaboration and customization of NGCs, free of bacteria, with pores in the internal surface, and the uniform wall thickness allowed manipulation, which showed flexibility; additionally, the sample was suturable. The NGCs showed initial biocompatibility with Schwann cells, revealing cells adhered to the NGC structure after 5 days. CONCLUSIONS: The fabricated CA/SPAH NGCs showed adequate features to be used for peripheral nerve regeneration studies. Future reports are necessary to discuss the ideal concentration of CA and SPAH and the mechanical and physicochemical properties of this biomaterial.

Unraveling the non-fitness status of NK cells: Examining the NKp30 receptor and its isoforms distribution in HIV/HCV coinfected patients.

BACKGROUND: HIV/HCV coinfection is associated with a rapid progression to liver damage. Specifically, NK cell population dysregulation is of particular interest, as these cells have been shown to block HCV replication effectively and have an anti-fibrogenic activity. The NKp30 receptor is linked to tumor cell lysis and has a crucial role during viral infections. In the present study, we determined the subpopulations of NK cells based on CD56 and CD16 expression, NKp30 receptor expression, its isoforms A, B, and C, along with the cytotoxicity molecules in patients with HIV/HCV. RESULTS: evidenced by the APRI and FIB-4 indices, the HCV-infected patients presented greater liver damage than the HIV and HIV/HCV groups. The HCV group presented a decreased expression of NKp30 isoform A, and NK cell frequency was not different between groups; however, CD56brigth subpopulation, NKp30 receptor, and CD247 adaptor chain were decreased in HIV/HCV patients; further, we described increased levels of soluble IL-8, IL-10, IL-12, and IL-23 in the serum of HIV/HCV patients. CONCLUSIONS: HCV and HIV/HCV patients have multiple parameters of non-fitness status in NK cells; awareness of these dysfunctional immunological parameters in HIV/HCV and HCV patients can elucidate possible novel therapeutics directed towards the improvement of NK cell fitness status, in order to improve their function against liver damage.

Modulation of Kynurenic Acid Production by N-acetylcysteine Prevents Cognitive Impairment in Adulthood Induced by Lead Exposure during Lactation in Mice.

Lead (Pb2+) exposure during early life induces cognitive impairment, which was recently associated with an increase in brain kynurenic acid (KYNA), an antagonist of NMDA and alpha-7 nicotinic receptors. It has been described that N-acetylcysteine (NAC) favors an antioxidant environment and inhibits kynurenine aminotransferase II activity (KAT II, the main enzyme of KYNA production), leading to brain KYNA levels decrease and cognitive improvement. This study aimed to investigate whether the NAC modulation of the brain KYNA levels in mice ameliorated Pb2+-induced cognitive impairment. The dams were divided into four groups: Control, Pb2+, NAC, and Pb2++NAC, which were given drinking water or 500 ppm lead acetate in the drinking water ad libitum, from 0 to 23 postnatal days (PNDs). The NAC and Pb2++NAC groups were simultaneously fed NAC (350 mg/day) in their chow from 0 to 23 PNDs. At PND 60, the effect of the treatment with Pb2+ and in combination with NAC on learning and memory performance was evaluated. Immediately after behavioral evaluation, brain tissues were collected to assess the redox environment; KYNA and glutamate levels; and KAT II activity. The NAC treatment prevented the long-term memory deficit exhibited in the Pb2+ group. As expected, Pb2+ group showed redox environment alterations, fluctuations in glutamate levels, and an increase in KYNA levels, which were partially avoided by NAC co-administration. These results confirmed that the excessive KYNA levels induced by Pb2+ were involved in the onset of cognitive impairment and could be successfully prevented by NAC treatment. NAC could be a tool for testing in scenarios in which KYNA levels are associated with the induction of cognitive impairment.

Exploring Schwann Cell Behavior on Electrospun Polyhydroxybutyrate Scaffolds with Varied Pore Sizes and Fiber Thicknesses: Implications for Neural Tissue Engineering.

The placement of a polymeric electrospun scaffold is among the most promising strategies to improve nerve regeneration after critical neurotmesis. It is of great interest to investigate the effect of these structures on Schwann cells (SCs), as these cells lead nerve regeneration and functional recovery. The aim of this study was to assess SC viability and morphology when cultured on polyhydroxybutyrate (PHB) electrospun scaffolds with varied microfiber thicknesses and pore sizes. Six electrospun scaffolds were obtained using different PHB solutions and electrospinning parameters. All the scaffolds were morphologically characterized in terms of fiber thickness, pore size, and overall appearance by analyzing their SEM images. SCs seeded onto the scaffolds were analyzed in terms of viability and morphology throughout the culture period through MTT assay and SEM imaging. The SCs were cultured on three scaffolds with homogeneous smooth fibers (fiber thicknesses: 2.4 µm, 3.1 µm, and 4.3 µm; pore sizes: 16.7 µm, 22.4 µm, and 27.8 µm). SC infiltration and adhesion resulted in the formation of a three-dimensional network composed of intertwined fibers and cells. The SCs attached to the scaffolds maintained their characteristic shape and size throughout the culture period. Bigger pores and thicker fibers resulted in higher SC viability.

Relación entre la cuenta de Twitter @ascolcirugia y las publicaciones de la Revista Colombiana de Cirugía durante la pandemia / Relationship between the Twitter account @ascolcirugia and the publications of the Colombian Journal of Surgery during the pandemic

Introducción. Indicadores alternativos basados en la web 2.0 han tomado importancia para medir el impacto de la producción científica. Previamente se han demostrado correlaciones positivas entre indicadores tradicionales y alternativos. El objetivo de este trabajo fue evaluar la relación de estos indicadores en el campo de la cirugía de nuestro país.Métodos. Análisis retrospectivo de las publicaciones de la Revista Colombiana de Cirugía y "tweets" de la cuenta @ascolcirugia entre marzo 2020 y julio 2021. Se evaluaron comparativamente los artículos con y sin publicación en la cuenta @ascolcirugia. Se determinó la correlación entre indicadores alternativos e indicadores tradicionales de las publicaciones de la revista. Resultados. En total se revisaron 149 artículos y 780 "tweets"; tan sólo el 13,4 % (n=20) de los artículos tuvieron visibilidad en la cuenta @ascolcirugia, con una mediana de 2 "tweets" (RIQ 1-2) por artículo, siendo la mayoría de estos sobre temas de COVID-19 (85 % vs 10 %; p<0,001). Los artículos publicados en @ascolcirugia tuvieron una mayor mediana de descargas (220 vs 116; p<0,001) y citaciones (3,5 vs 0; p<0,001) en comparación con los que no fueron publicados.Conclusión. El uso de las redes sociales tiene un efecto positivo en el número de lectores de la Revista Colombiana de Cirugía y el impacto académico de los autores. Aunque existe una buena correlación entre indicadores alternativos y tradicionales en el contexto nacional, la proporción de artículos de la Revista Colombiana de Cirugía publicados en la cuenta @ascolcirugia es baja.

Introduction. Alternative indicators based on web 2.0 have gained great relevance to measure the impact of scientific production. Positive correlations between traditional and alternative indicators have previously been shown. The objective of our article is to evaluate the relationship of these indicators in the field of surgery in our country.Methods. Retrospective analysis of the publications of the Colombian Journal of Surgery and tweets of the Twitter account (@ascolcirugia) during March 2020 and July 2021. Articles with and without tweets in the account @ascolcirugia were comparatively evaluated. The correlation between alternative indicators and traditional indicators of the journal's publications was determined. Results. A total of 149 articles and 780 tweets were reviewed; only 13.4% (n=20) of the articles had visibility at the @ascolcirugia account, with a median of 2 tweets (RIQ 1-2) per article, most of which were on COVID-19 issues (85% vs 10%; p<0.001). The articles published at the @ascolcirugia account had a higher median number of downloads (220 vs 116; p<0.001) and citations (3.5 vs 0; p<0.001) compared to the articles that were not published. Conclusions. The use of social media has a positive effect on the number of readers of the Colombian Journal of Surgery and the academic impact of the authors. Although there is a good correlation between alternative and traditional indicators, in the national context, the proportion of articles of the Colombian Journal of Surgery published at the @ascolcirugia account is low

Cross-Cultural Adaptation of the Children's Emotion Regulation Processes Survey (CERP) in Chilean Preschoolers / Adaptação Transcultural do Questionário de Processos de Regulação Emocional (CERP) em Pré-Escolares Chilenos / Adaptación Transcultural del Cuestionario de Procesos de Regulación Emocional (CERP) en una Muestra de Preescolares Chilenos

Abstract Emotion regulation is an important aspect for the adaptation of human beings that influences their development and mental health. In actuality, it is an important challenge to have culturally valid instruments that allow to evaluate this construct. In this study, an analysis of the psychometric properties of the Children's Emotion Regulation Processes Survey (CERP) in a sample of Chilean preschoolers is presented. CERP is a third-party report questionnaire that assesses the strategies of emotion regulation in children, providing a vision based on development. The instrument was applied to mothers and fathers of 483 children, between the ages of 4 and 6 that attended preschool. Factorial structure, internal consistency and the relation with the Child Behavior Checklist (CBCL) as indicator of convergent validity was analyzed. The results showed a four factor structure, coherent with the empirical evidence. Also, all of the factors presented adequate reliability indexes and correlation with the internalization and externalization scales of CBCL. It is concluded that CERP exhibits positive reliability and validity indicators to be used in this population.

Resumo A regulação emocional é um aspecto de relevância para a adaptação dos indivíduos que influencia em seu desenvolvimento e saúde mental. Na atualidade, um desafio importante é contar com instrumentos válidos culturalmente que permitam avaliar este constructo. Neste estudo se apresenta a análise das propriedades psicométricas do Questionário de Processos de Regulação Emocional Infantil (CERP) em uma amostra de pré-escolares chilenos. O CERP é um questionário de relatórios de terceiros que avaliam estratégias de regulação emocional em crianças, entregando uma visão baseada no desenvolvimento. O instrumento foi aplicado a pais e mães de 483 crianças, entre 4 e 6 anos que cursavam a educação pré-escolar. Foi analisada a estrutura fatorial, consistência interna e o relacionamento com o Inventario de Condutas Infantis (CBCL) como indicador de validade convergente. Os resultados evidenciaram uma estrutura de quatro fatores, coerentes com a evidencia empírica. Além disso, todos os fatores apresentaram índices adequados de confiabilidade e correlações com as escalas de internalização e terceirização do CBCL. Se conclui que o CERP mostra indicadores de confiabilidade e validez positivos para serem usados nesta população.

Resumen La regulación emocional es un aspecto de relevancia para la adaptación de los individuos que influye en su desarrollo y salud mental. Actualmente, es un desafío contar con instrumentos válidos culturalmente para evaluar este constructo. En este estudio, se presenta el análisis de las propiedades psicométricas del Cuestionario de Procesos de Regulación Emocional Infantil (CERP) en una muestra de preescolares chilenos. El CERP es un cuestionario de reporte de terceros que evalúa las estrategias de regulación emocional en niños/as, entregando una visión basada en el desarrollo. Se aplicó el instrumento a padres y madres de 483 niños/as de edades entre 4 y 6 años que cursaban educación preescolar. Se analizó la estructura factorial, consistencia interna y relación con el Inventario de Conductas Infantiles (CBCL) como indicador de validez convergente. Los resultados evidenciaron una estructura de cuatro factores, coherentes con la evidencia empírica. Además, todos los factores presentaron adecuados índices de confiabilidad y correlaciones con las escalas de internalización y externalización del CBCL. Se concluye que el CERP muestra indicadores de confiabilidad y validez positivos para ser usado en esta población.

Percepciones y actitudes de los pacientes frente a la cirugía de la epilepsia: conceptos equivocados en Colombia / Patients' perceptions of and attitudes towards epilepsy surgery: mistaken concepts in Colombia

Introducción. Los pacientes con epilepsia focal farmacorresistente se podrían beneficiar de la cirugía de la epilepsia; sin embargo, existen demoras en la realización del procedimiento. El objetivo de este estudio fue evaluar el conocimiento y las actitudes de los pacientes hacia la cirugía de epilepsia e identificar barreras que pudieran retrasar el tratamiento. Pacientes y métodos. Se aplicó un cuestionario de 10 minutos a pacientes con epilepsia en Colombia. La encuesta evaluó el conocimiento de la opción quirúrgica, las percepciones sobre el riesgo de la cirugía frente al riesgo de crisis no controladas, la discapacidad producida por la enfermedad, las metas del tratamiento y las variables demográficas y socioeconómicas. Resultados. Se seleccionaron 88 pacientes con epilepsia focal. El 56% de los pacientes no sabía que la cirugía podría ser una opción terapéutica. El 60% consideró que la cirugía de la epilepsia es muy o moderadamente peligrosa. Una gran proporción pensaba que la muerte (41%), el ictus (47%), la pérdida visual (56%), los cambios en la personalidad (56%), la parálisis (61%), las dificultades para hablar (69%) y la pérdida de la memoria (60%) eran efectos secundarios comunes. La mayoría (62%) consideraba el procedimiento como la última opción de tratamiento. Conclusiones. Existe una actitud negativa por parte de los pacientes frente a la cirugía de la epilepsia fundamentada en la sobreestimación del riesgo de adquirir déficits neurológicos secundarios al procedimiento, lo que refleja la falta de conocimiento hacia este tratamiento. Estas percepciones erróneas pueden contribuir a demoras en la atención quirúrgica

Introduction. Selected patients with drug-resistant focal epilepsy benefit from epilepsy surgery, however significant delays remain. The aim of this study was to assess knowledge and attitudes toward epilepsy surgery among patients with epilepsy and identify barriers that might delay the treatment. Patients and methods. A 10-minute questionnaire was administered to patients with epilepsy in Colombia. Survey assessed the following: knowledge of surgical options, perceptions about the risks of surgery vs. ongoing seizures, disease disability, treatment goals, and demographic and socioeconomic variables. Results. We recruited 88 patients with focal epilepsy. More than half of patients (56%) were not aware that surgery might be an option. Apprehension about epilepsy surgery was evident, 60% of patients perceived epilepsy surgery to be very or moderately dangerous. A large proportion of patients believe death (41%), stroke (47%), vision loss (56%), personality change (56%), paralysis (62%), difficulties in speaking (69%), and memory loss (60%) were frequent side effects. The majority of patients (62%) consider the surgical procedure as the last option of treatment. Conclusions. There is a negative attitude toward epilepsy surgery based on the patients' misperceptions of suffering neurological deficits during the surgery, reflecting lack of knowledge toward this type of treatment. These perceptions can contribute to delays in surgical care

Caracterización del apoyo social percibido durante el posparto y la presencia de depresión materna / Characterization of Perceived Social Support During Postpartum and the Presence of Maternal Depression

Introducción: el período de posparto es una etapa de vulnerabilidad biopsicosocial en la mujer. Se reconoce una prevalencia de depresión posparto en Chile de un 27,2 por ciento a un 41,3 por ciento. Por otra parte, el apoyo social puede ser un factor protector para este periodo. Objetivo: relacionar las características del apoyo social percibido y la presencia de depresión materna en el periodo de posparto. Métodos: estudio transversal correlacional, de 76 mujeres en periodo de posparto (30 días a 1 año), sanas y sin antecedentes de depresión. Se les evaluó sintomatología depresiva, percepción del apoyo social y funcionamiento familiar. Se agregó un cuestionario de variables biosociales, obstétricas y relacionales, previo consentimiento informado y autorización del comité de ética del centro de salud. El análisis se realizó con el programa estadístico SPSS versión 19.0. Se aplicó un análisis descriptivo univariado, bivariado y multivariado con un nivel de significación estadística menor de 0,05. Resultados: la depresión fue detectada en 27,6 por ciento de las puérperas. La asociación entre la presencia de depresión posparto con la percepción del apoyo social, resultaron estadísticamente significativas al igual que la funcionalidad familiar y la percepción de la relación con la pareja y la madre. Las variables biosociales y obstétricas no resultaron significativas para la presencia de depresión. Conclusiones: los factores protectores de la depresión materna identificados fueron: el apoyo social percibido, el funcionamiento familiar y aspectos relacionales con la pareja y la madre(AU)

Introduction: The postpartum period is a time of bio-psychosocial vulnerability in women, recognizing the prevalence of postpartum depression in Chile from 27.2 percent to 41.3 percent. Moreover, social support may be a protective factor for this period. Objective: to relate the characteristics of perceived social support and the presence of maternal depression in the postpartum period. Methods: A correlational cross-sectional study was led on 76 healthy women in postpartum period (30 days to 1 year), and without a history of depression. Depressive symptomatology, social support perception, and family functioning were evaluated. A questionnaire of biosocial, obstetrical and relational variables was added, with prior informed consent and authorization from the ethics committee of the health center. The statistical analysis was performed with SPSS version 19.0, using descriptive univariate, bivariate and multivariate a statistical significance level less than 0.05. Results: Postpartum depression was detected in a 27, 6 percent. The association between depressions with social support perception was statistically significant, as family functioning and the relationship with their partners and mothers. Biosocial and obstetric variables were not significant for the presence of depression. Conclusions: Perceived social support, family functioning and relational aspects with their partners and mothers are protective factors for maternal depression, compared with other variables(AU)

Estudio epidemiológico sobre juegos de azar y factores asociados en poblacion universitariade la universidad de Manizales, Colombia, 2010 / Epidemiological study of gambling games and associated factors in the university population, of Manizales University, Colombia 2010

Antecedentes: Los juegos de azar se definen como toda actividad que consista en poner en riesgo un objeto de valor con la esperanza de obtener otro de mayor cuantía. El juego patológico constituye actualmente un importante problema de salud mental que acarrea un gran número de problemas a nivel personal, familiar y social. Materiales y métodos: En el presente estudio se analiza la frecuencia de práctica de juegos de azar y factores relacionados en estudiantes de pregrado de la universidad de Manizales en el año 2010, en una muestra seleccionada de 305 estudiantes en donde se analizaron variables como género, edad, programa, estrato socioeconómico, ingreso semanal, tipo de juego y su frecuencia, consumo de alcohol, cigarrillo, drogas, función familiar entre otras. Se utilizó para la evaluación del tipo de jugador el cuestionario de F.A.J.E.R (No jugador, Jugador con riesgo, Jugador dependiente). Resultados: Los resultados indican que 53,3% practican algun juego de azar. Según el cuestionario de F.A.J.E.R, un 7,6% y se clasificaron como jugadores dependientes, 13,6% jugadores en riesgo. Se encontró asociación significativa entre tipo de jugador y genero (p=0,000), programa (p=0,017), antecedentes de familiares de juego patológico(p=0,000), consumo de alcohol (p=0,000), funcionalidad familiar (p=0,011), consumo de sustancias psicoactivas (p=0,020), tabaquismo (p=0,042). Conclusiones: Existe una importante frecuencia de la práctica de juegos de azar en la población estudiada y el juego patológico merece más atención desde la perspectiva de la salud...

Tolerancia, pluralismo y fe en el trabajo académico en Bioética / Tolerancia, pluralism and faith in the academic work in Bioética

Partiendo de la pregunta sobre el modo en que la identidad cristiana debe resplandecer en la Facultad de Medicina de una institución educativa que se confiese oficialmente católica (como la nuestra), la autora reflexiona sobre la posibilidad de entablar un diálogo tolerante y pluralista, precisamente desde la identidad cristiana. En el desarrollo de esta reflexión se intenta mostrar que un católico debe ser tolerante, pues esta actitud viene solicitada por postulados esenciales de la fe que profesa. Se propone, además, un modo de cultivar una actitud abierta y tolerante en el trabajo académico en general y, más concretamente, en la enseñanza de la Bioética al interior de una Facultad de Medicina católica.

Departing from the question concerning the way in which the catholic identity should shine in the Medical School of an educational institution that is declared to be officially catholic, the author reflects on the possibility of engaging in a dialogue that would be tolerant and pluralistic, coming precisely from the Christian identity. Unfolding this reflection, the author tries to show that a catholic ought to be tolerant, as this attitude is demanded by the very faith he/she holds. A proposal is made as how to cultivate an open and tolerant attitude in the academic work in general, and more concretely, in the teaching of Bioethics in a Catholic Medical School.