Combined loss of p21(waf1/cip1) and p27(kip1) enhances tumorigenesis in mice.

The cell cycle inhibitors p21(Waf1/Cip1) and p27(Kip1) are frequently downregulated in many human cancers, and correlate with a worse prognosis. We show here that combined deficiency in p21 and p27 proteins in mice is linked to more aggressive spontaneous tumorigenesis, resulting in a decreased lifespan. The most common tumors developed in p21p27 double-null mice were endocrine, with a higher incidence of pituitary adenomas, pheochromocytomas and thyroid adenomas. The combined absence of p21 and p27 proteins delays the incidence of radiation-induced thymic lymphomas with a higher apoptotic rate, measured by active caspase-3 and cleaved PARP-1 immunoexpresion. These results provide experimental evidence for a cooperation of both cyclin-dependent kinase inhibitors in tumorigenesis in mice.

Lesions associated with the plexus venosus subcutaneus collaris of pigeons with chlorophacinone toxicosis.

We describe lesions in 29 adult domestic pigeons (Columba livia) poisoned with chlorophacinone, an indandione anticoagulant rodenticide. Birds were found dead in the field and in dovecotes after a wide-area treatment against common voles (Microtus arvalis) based on the dispersion in the open field of wheat grain treated with 0.005% chlorophacinone. At necropsy, most pigeons showed crops full of red-colored wheat grain, extensive subcutaneous hematoma in neck and breast zones, and hemorrhages in lungs and the coelomic cavity. Chlorophacinone was determined in liver samples by high-performance liquid chromatography with ultraviolet detection, and the mean (range) concentration was 11.2 (1.48-50.1) microg/g. Pigeons have a venous subcutaneous plexus in the neck zone (plexus venosus subcutaneus collaris), which has been described as an erectile tissue with a thermoregulation function. This case report describes a relationship between the subcutaneous hemorrhagic lesions and the plexus venosus collaris.

Hepatic cells via cava vein can influence allogenic islet rat transplantation.

We have reported, previously, some effect of allogenic hepatic cells for islet tolerance when they are injected mixed (hepatic cells and islets) in different proportions via portal vein, in diabetic Wistar rats. Now we have studied the role of allogenic hepatic cells injected sequentially 15 min before islets, comparing via the portal vein (A and B groups) and via the cava vein (C and D groups) with a control group of islets alone. The allogenic islets were always injected via portal vein, in similar conditions, while the ratio of hepatic cells/islets was 100:1 (A, C groups) or 200:1 (B, D groups). Islets and hepatic cells were obtained from several different rats. The transplanted rats were observed during 30 days and results compared among the different rat groups: porta-porta (P/P), cava-porta (C/P), and control group. Statistically, a significant interaction between type of transplant and proportion of hepatic cells was observed. Also, C plus D groups showed statistical difference with the control group (p < 0.017) and also all the groups together (p < 0.047). These results suggest that hepatic cells can induce, in some cases, islet graft prolongation in Wistar rats. Better results were obtained when hepatic cells were injected via the cava vein than via the portal vein. Because we used a liver cell suspension integrated for several kinds of cells, the study does not clarify if this effect can be related to some specific hepatic cell subpopulation. To confirm the results and to determine if the hypothetical mechanism can be attributed to a block of the immune system or to some factor secreted by hepatic cells, more studies must be performed.

Neonatal mortality in puppies due to bacteremia by Streptococcus dysgalactiae subsp. dysgalactiae.

We report a case of bacteremia in puppies caused by Streptococcus dysgalactiae subsp. dysgalactiae. Identification was achieved by phenotypic and molecular genetic methods. This is the first report of the recovery of S. dysgalactiae subsp. dysgalactiae from dogs.

Pseudomonas simiae sp. nov., isolated from clinical specimens from monkeys (Callithrix geoffroyi).

An unusual Gram-negative, catalase- and oxidase-positive, rod-shaped bacterium isolated from different clinical samples from two monkeys (Callithrix geoffroyi) was characterized by phenotypic and molecular genetic methods. The micro-organism was tentatively identified as a Pseudomonas species on the basis of the results of cellular morphological and biochemical tests. Fatty acid studies confirmed this generic placement and comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were phylogenetically closely related to each other (100% sequence similarity) and were part of the 'Pseudomonas fluorescens intrageneric cluster'. The novel bacterium, however, was distinguished from other phylogenetically related species of Pseudomonas by DNA-DNA hybridization studies and biochemical tests. On the basis of both phenotypic and phylogenetic findings, it is proposed that the novel Pseudomonas isolates are classified as Pseudomonas simiae sp. nov. The type strain of P. simiae is OLiT (=CCUG 50988T=CECT 7078T).