Detection of Known and Novel Small Proteins in Pseudomonas stutzeri Using a Combination of Bottom-Up and Digest-Free Proteomics and Proteogenomics.

Small proteins of around 50 aa in length have been largely overlooked in genetic and biochemical assays due to the inherent challenges with detecting and characterizing them. Recent discoveries of their critical roles in many biological processes have led to an increased recognition of the importance of small proteins for basic research and as potential new drug targets. One example is CcoM, a 36 aa subunit of the cbb3-type oxidase that plays an essential role in adaptation to oxygen-limited conditions in Pseudomonas stutzeri (P. stutzeri), a model for the clinically relevant, opportunistic pathogen Pseudomonas aeruginosa. However, as no comprehensive data were available in P. stutzeri, we devised an integrated, generic approach to study small proteins more systematically. Using the first complete genome as basis, we conducted bottom-up proteomics analyses and established a digest-free, direct-sequencing proteomics approach to study cells grown under aerobic and oxygen-limiting conditions. Finally, we also applied a proteogenomics pipeline to identify missed protein-coding genes. Overall, we identified 2921 known and 29 novel proteins, many of which were differentially regulated. Among 176 small proteins 16 were novel. Direct sequencing, featuring a specialized precursor acquisition scheme, exhibited advantages in the detection of small proteins with higher (up to 100%) sequence coverage and more spectral counts, including sequences with high proline content. Three novel small proteins, uniquely identified by direct sequencing and not conserved beyond P. stutzeri, were predicted to form an operon with a conserved protein and may represent de novo genes. These data demonstrate the power of this combined approach to study small proteins in P. stutzeri and show its potential for other prokaryotes.

Proteomic Characterization of the Pseudomonas sp. Strain phDV1 Response to Monocyclic Aromatic Compounds.

The degradation of aromatic compounds comprises an important step in the removal of pollutants and re-utilization of plastics and other non-biological polymers. Here, Pseudomonas sp. strain phDV1, a gram-negative bacterium that is selected for its ability to degrade aromatic compounds is studied. In order to understand how the aromatic compounds and their degradation products are reintroduced in the metabolism of the bacteria and the systematic/metabolic response of the bacterium to the new carbon source, the proteome of this strain is analyzed in the presence of succinate, phenol, and o-, m-, and p-cresol as the sole carbon source. As a reference proteome, the bacteria are grown in succinate and then compared with the respective proteomes of bacteria grown on phenol and different cresols. In total, 2295 proteins are identified; 1908 proteins are used for quantification between different growth conditions. The carbon source affects the synthesis of enzymes related to aromatic compound degradation and in particular the enzyme involved in the meta-pathway of monocyclic aromatic compounds degradation. In addition, proteins involved in the production of polyhydroxyalkanoate (PHA), an attractive biomaterial, show higher abundance in the presence of monocyclic aromatic compounds. The results provide, for the first time, comprehensive information on the proteome response of this strain to monocyclic aromatic compounds.

Proteome Analysis of Enriched Heterocysts from Two Hydrogenase Mutants from Anabaena sp. PCC 7120.

Cyanobacteria are oxygenic photosynthetic prokaryotes and play a crucial role in the Earth's carbon and nitrogen cycles. The photoautotrophic cyanobacterium Anabaena sp. PCC 7120 has the ability to fix atmospheric nitrogen in heterocysts and produce hydrogen as a byproduct through a nitrogenase. In order to improve hydrogen production, mutants from Anabaena sp. PCC 7120 are constructed by inactivation of the uptake hydrogenase (ΔhupL) and the bidirectional hydrogenase (ΔhoxH) in previous studies. Here the proteomic differences of enriched heterocysts between these mutants cultured in N2 -fixing conditions are investigated. Using a label-free quantitative proteomics approach, a total of 2728 proteins are identified and it is found that 79 proteins are differentially expressed in the ΔhupL and 117 proteins in the ΔhoxH variant. The results provide for the first time comprehensive information on proteome regulation of the uptake hydrogenase and the bidirectional hydrogenase, as well as systematic data on the hydrogen related metabolism in Anabaena sp. PCC 7120.

Establishing and Maintaining Social Relationships During Significant Life Events: The Role of Age.

OBJECTIVES: We investigate how adults of different ages experience changes in their social relationships during significant life events. Based on different goal priorities, younger adults may benefit more from establishing new social contacts during a significant life event, whereas older adults may benefit more from maintaining existing relationships. METHODS: To test these hypotheses, we conducted multilevel modelling with a sample of N = 6,688 participants aged 18 to 90 years who reported at least one significant life event in the past two years. RESULTS: Both establishing new social relationships and maintaining existing relationships during significant life events were associated with higher levels of well-being. As predicted, these associations were moderated by age. Establishing new social relationships was more strongly associated with mental health and life satisfaction in younger adults, whereas maintaining existing relationships was more strongly associated with subjective well-being, physical health, and loneliness in older adults. DISCUSSION: These findings provide valuable insights into the role of age in the change and stability of social relationships during significant life events.

Are social interactions perceived as more meaningful in older adulthood?

Social relationships accompany us throughout adulthood and are among the most important sources of meaning in a person's life. However, little is known about age differences in meaningfulness of social interactions across adulthood. According to socioemotional selectivity theory, as people age, they develop relatively stronger preferences for social relationships that are emotionally meaningful. Consequently, older adults may perceive social interactions in everyday life as more meaningful than younger adults. To test this hypothesis, the present study examined age-related differences in the perceived meaningfulness of daily social interactions using experience sampling data. Three-hundred six participants (56.9% women, 18-88 years) completed a total of 6,407 entries over 3 days. Results of the multilevel analyses showed that age was positively associated with perceived meaningfulness of daily social interactions, controlling for relationship closeness and situation valence. In addition, the perceived meaningfulness of daily social interactions was positively related to subjective well-being both between and within all participants, indicating that meaningful social interactions are beneficial for well-being regardless of age. Thus, perceiving social interactions as meaningful could be one way that older people maintain a high level of well-being in their daily lives. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Feeling Younger on Active Summer Days? On the Interplay of Behavioral and Environmental Factors With Day-to-Day Variability in Subjective Age.

Background and Objectives: Subjective age, that is, how old people feel in relation to their chronological age, has mostly been investigated from a macro-longitudinal, lifespan point of view and in relation to major developmental outcomes. Recent evidence also shows considerable intraindividual variations in micro-longitudinal studies as well as relations to everyday psychological correlates such as stress or affect, but findings on the interplay with physical activity or sleep as behavioral factors and environmental factors such as weather conditions are scarce. Research Design and Methods: We examined data from 80 recently retired individuals aged 59-76 years (M = 67.03 years, 59% women) observed across 21 days. Daily diary-based assessments of subjective age, stress, affect, and sleep quality alongside physical activity measurement via Fitbit (steps, moderate-to-vigorous physical activity) and daily hours of sunshine were collected and analyzed using multilevel modeling. Results: Forty-four percent of the overall variance in subjective age was due to intraindividual variation, demonstrating considerable fluctuation. Affect explained the largest share in day-to-day fluctuations of subjective age, followed by stress and steps, whereas sunshine duration explained the largest share of variance in interindividual differences. Discussion and Implications: In our daily diary design, subjective age was most strongly related to self-reported affect as a psychological correlate. We, however, also found clear associations with objective data on daily steps and weather. Hence, our study contributes to contextualizing and understanding variations in subjective age in everyday life.

Successful Aging and Subjective Aging: Toward a Framework to Research a Neglected Connection.

Research related to subjective aging, which describes how individuals perceive, interpret and evaluate their own aging, has substantially grown in the past two decades. Evidence from longitudinal studies shows that subjective aging predicts health, quality of life, and functioning in later life. However, the existing literature on successful aging has mostly neglected the role of subjective aging. This paper proposes an extended framework of successful aging linking subjective aging conceptually and empirically to Rowe and Kahn's (1997) three original key criteria of successful aging (i.e., avoiding disease and disability, maintaining high cognitive and physical function, and engagement with life). A particular focus is placed on subjective aging as an antecedent of successful aging. A review of the empirical subjective aging literature shows that subjective aging concepts consistently predict all three of Rowe and Kahn's criteria of successful aging. Mechanisms underlying these relations are discussed at three levels, namely psychological, behavioral, and physiological pathways. The proposed addition also takes into consideration the interconnections between subjective aging and successful aging throughout the lifespan and across historical time. Finally, we discuss the importance of facilitating successful aging through systematic interventions that support more positive views of aging at the individual and societal level.

VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity.

Synapses are pivotal sites of plasticity and memory formation. Consequently, synapses are energy consumption hotspots susceptible to dysfunction when their energy supplies are perturbed. Mitochondria are stabilized near synapses via the cytoskeleton and provide the local energy required for synaptic plasticity. However, the mechanisms that tether and stabilize mitochondria to support synaptic plasticity are unknown. We identified proteins exclusively tethering mitochondria to actin near postsynaptic spines. We find that VAP, the vesicle-associated membrane protein-associated protein implicated in amyotrophic lateral sclerosis, stabilizes mitochondria via actin near the spines. To test if the VAP-dependent stable mitochondrial compartments can locally support synaptic plasticity, we used two-photon glutamate uncaging for spine plasticity induction and investigated the induced and adjacent uninduced spines. We find VAP functions as a spatial stabilizer of mitochondrial compartments for up to ~60 min and as a spatial ruler determining the ~30 µm dendritic segment supported during synaptic plasticity.

Willingness to Live Under Impairment: Factors Associated With Desired Longevity.

Background and Objectives: Longevity is a societal achievement. However, people might not wish to live long lives under all conditions. When deciding about their longevity desires, some individuals may focus on present-oriented, concrete aspects of their lives, like their current state of health, whereas others may weigh up more future-oriented, abstract aspects, such as how important it is to be healthy. We investigated what factors are associated with individuals' willingness to live with impairment. Research Design and Methods: Factors associated with willingness to live with impairment were examined in a sample of N = 790 German participants aged 39-90 years (Mage = 63.38, SD = 14.30, 51% female). To examine age-specific factors related to desired longevity under impairment conditions, chronological age was added as a moderator in the analyses. Results: Findings indicated that for middle-aged adults, fear of becoming a burden and higher importance of health were associated with lower willingness to live with impairment. For older adults, lower willingness to live with impairment was associated with higher levels of instrumental preparation for the end of life. Discussion and Implications: The obtained age differences can be understood as reflecting a shift in time perspective and personal experiences. Among middle-aged adults, willingness to live with impairment is more strongly influenced by distant events that pertain to the future (e.g., fear of becoming a burden). Older adults, however, place more importance on their current life situation (e.g., instrumental preparation), and their willingness to live with impairment seems to be more conditional on practical aspects in the present.

Differences in self-perceptions of aging across the adult lifespan: The sample case of awareness of age-related gains and losses.

Rooted in the premises of lifespan developmental theory, the concept of awareness of age-related change (AARC) posits that growing older comes with both experiences of gains and losses across different behavioral domains. However, little is known about how age-related change is perceived across the entire adult lifespan, provided that respective measures can be validly compared. Further, few studies have adopted an approach that examines gains and losses simultaneously to study a potential shift in the ratio of perceived age-related gains and losses from adolescence to advanced old age. Using cross-sectional data from the German Socio-Economic Panel, this study tested the measurement invariance of the 10-item AARC short form and examined age differences in the awareness of age-related changes across 1,612 participants aged 16-93 years. First, partial measurement invariance of the AARC-Gains and AARC-Losses scales was established, allowing for valid group comparisons across young adulthood, midlife, and old age. Second, results indicated that people experience more AARC-Gains than AARC-Losses throughout the adult lifespan. However, older adults exhibited an increasingly less favorable gains-to-losses ratio, primarily driven by more loss experiences. Gain experiences were mostly stable across age groups. Third, differences in levels of AARC were related to individuals' background characteristics relevant at the respective time of life, such as education (early adulthood), employment (midlife), and social resources (old age). These results highlight the utility of considering a broad age range when examining the nature and correlates of age differences in perceived age-related gains and losses. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Levels of awareness of age-related gains and losses throughout adulthood and their developmental correlates.

Views of aging predict key developmental outcomes. Less is known, however, about the consequences of constellations of domain-specific perceived gains and losses across the full adult lifespan. First, we explored levels of awareness of age-related gains (AARC-gains) and losses (AARC-losses) in five behavioral domains across adulthood. Second, we identified the number and types of profiles of AARC-gains and AARC-losses in young adulthood, midlife, young-old age, and old-old age. Third, we investigated whether the identified profiles differed in their associations with developmental correlates. Data came from the 2018 German Socio-Economic Panel Innovation Sample (SOEP-IS), comprising 403 young, 721 middle-aged, 260 young-old and 228 old-old individuals. We assessed AARC, physical and mental functioning, information processing speed, social relations, lifestyle, and engagement. At the sample level, AARC-losses were higher in old age, whereas AARC-gains did not differ across adulthood. Latent profile analyses revealed two distinguishable constellations of AARC-gains and AARC-losses that characterize young adulthood and old-old age, whereas four and three gains-to-losses constellations are needed to characterize midlife and young-old age, respectively. In middle, young-old, and old-old age, profiles with more AARC-losses were associated with poorer scores on all developmental correlates. Overall, study results suggest that age-related experiences are most diversified in midlife and young-old age. Asking individuals about their negative age-related experiences may help identify those individuals who are doing less well in important developmental correlates. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Assessing psychological variables on mobile devices: an introduction to the experience sampling app ESM-Quest.

The use of the Experience Sampling Method (ESM), which involves repeated assessments in people's daily lives, has increased in popularity in psychology and associated disciplines in recent years. A rather challenging aspect of ESM is its technical implementation. In this paper, after briefly introducing the history of ESM and the main reasons for its current popularity, we outline the ESM-Quest experience sampling app which is currently being developed at the University of Vienna. ESM-Quest runs on different operating systems, specifically on mobile devices such as smartphones and tablets running either iOS or Android. An internet connection is not necessary during the assessment. Compared to most other ESM apps, ESM-Quest allows event-based random sampling, which is very helpful when assessments need to be collected within specific situations. Currently, ESM-Quest is being utilized at the University of Vienna and will be made available for research groups worldwide upon request. We introduce the technical aspects of ESM-Quest and provide examples of analyses on ESM data collected through this app, such as examining fluctuations in constructs within individuals. Finally, we outline potential next steps in ESM research.

Experiences of solitude in adulthood and old age: The role of autonomy.

Recent evidence suggests that older adults experience momentary states of spending time alone (i.e., solitude) less negatively than younger adults. The current research explores the role of autonomy as an explanation mechanism of these age differences. Previous research demonstrated that solitude can be experienced positively when it is characterized by autonomy (i.e., the own wish or decision to be alone). As older adults are relatively more autonomous in their daily lives, they might experience solitude less negatively (in terms of subjective well-being, social integration, self-esteem, and valence) than younger adults. We tested this hypothesis in three studies. In two experience-sampling studies (Study 1: N = 129, 59.7% women, age 19-88 years; Study 2: N = 115, 66.4% women, age 18-85 years), older age and higher autonomy were associated with more positive experience of everyday solitude moments. Although autonomy did not differ between younger and older adults, perceived (lack of) autonomy partly played a more important role for the experience of solitude moments in younger adults compared to older adults. Finally, Study 3 (N = 323, 52% women, age 19-79 years) showed that the relationship between recalled solitude moments of high versus low autonomy and solitude experience is fully explained by feelings of autonomy. Overall, our results demonstrate that older people do not experience more autonomy in situations of solitude than younger adults, but that they partly better cope with low-autonomy solitude. However, people of all ages seem to benefit more from high-autonomy moments of solitude.

Aging-related fears and their associations with ideal life expectancy.

Fears regarding various aspects tend to stimulate individuals to escape or to avoid the sources of the threat. We concluded that fears associated with the future aging process, like the fear of aging-related diseases, the fear of loneliness in old age, and the fear of death, would stimulate patterns of avoidance when it comes to ideal life expectancy. We expected fear of aging-related diseases and fear of loneliness in old age to be related to lower ideal life expectancies. We expected fear of death to be related to higher ideal life expectancies. In two adult lifespan samples [N 1 = 1065 and N 2 = 591; ages ranging from 18 to 95 years, M (SD)1 = 58.1 (17.2) years, M (SD)2 = 52.6 (18.1) years], we were able to support our hypothesis regarding fear of death. We furthermore found significant interactions among the fears, indicating that individuals fearing diseases or loneliness but being unafraid of death opted for the shortest lives. Our results indicate that fears regarding life in very old age might be associated with the wish to avoid this age period; the fear of death was however associated with the wish for particularly long lives, and thus, with distancing oneself from the dreaded event of death. We conclude that fears seem to be associated with how individuals approach old age and with what they wish for in their own future as aged people.

COVID-19 and perceiving finitude: Associations with future time perspective, death anxiety, and ideal life expectancy.

The coronavirus pandemic threatens the health, future, and life of individuals and might hence accentuate perceptions of the fragility and finitude of life. We investigated how different perceptions of the pandemic (regarding the virus as a health threat and perceiving social and financial restrictions due to the pandemic) relate to different perceptions of life's finitude (i.e., future time perspective, death anxiety, and ideal life expectancy). Using longitudinal data from 1,042 adults (68% women; aged 18-95 years) gathered within the first and within the second peak of the pandemic in Germany, we expected decreases in future time perspective and ideal life expectancy, as well as increases in death anxiety in response to threatening perceptions of the pandemic. The results indicated decreasing future time perspectives, an accentuation of death anxiety right at the beginning of the pandemic, as well as stable ideal life expectancies. There was a tendency for more pronounced change among older adults. Initial levels and changes in the perceptions of finitude could partly be explained by initial and changing perceptions of the pandemic. Next to perceptions targeting the threat of the virus itself, perceptions of strong social and financial restrictions during the pandemic contributed to an altered stance toward the finitude of life. Concluding, we discuss stability and variation in perceptions of the finitude of life during a time of major societal change and a potentially life-threatening pandemic. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

COVID-19 Worries Predict Aging Preparation: Culture- and Domain-Specific Perspectives.

OBJECTIVES: We investigated whether worrying about coronavirus disease 2019 (COVID-19) predicts people's engagement in aging preparation. Furthermore, we expected that this association would have culture- (i.e., Hong Kong and Germany) and domain-specific (i.e., finances, housing, care needs, connectedness, and end-of-life) tendencies, as the culture and domains that are most severely hit by the pandemic differ. METHODS: A total of 360 and 1,294 adults (aged 18-98 years) living in Hong Kong and Germany, respectively, participated in a web-based study. We fitted our data to a multilevel model in order to take into account the interdependence of domains (i.e., Level 1) within the same individual (i.e., Level 2). RESULTS: The results revealed that reporting higher COVID-19 worries were associated with pandemic-induced aging preparation, and this association was particularly apparent for Germans in comparison to those from Hong Kong. When domains were specified, this cultural difference appeared significantly stronger for the domains of care, connectedness, and end-of-life than finances and housing. DISCUSSION: Findings imply that worrying about the COVID-19 pandemic predicts people to engage in aging preparation particularly in the culture and domains most affected by the pandemic. These results from those worried about the virus may be attributed to the increased self-relevance to the topic and hence motivation.

Awareness of age-related change in the context of major life events.

Although gains and losses are an integral part of human development, the experience of change and readjustment that often comes with major life events may be particularly influential for an individual's subjective aging experience and awareness of age-related change (AARC). Thus, this study focused on the role of life events in the domains of family and health for an individual's awareness of age-related gains and losses. Specifically, we differentiated between the experience of specific life events (e.g., entering a new romantic relationship; hospital stay) and the cumulative experience of multiple life events. Furthermore, we differentiated between life events experienced at an expected time in life and life events experienced relatively early or relatively late compared to established social norms. Data came from the Innovation Sample of the German Socio-Economic Panel (SOEP-IS) and consisted of 1,612 participants aged 16 to 93 years (M = 54.1; SD = 18.2). Life events were assessed annually and retrospectively for the last 2 years. Propensity score matching provided evidence for an association of specific family life events and a higher awareness of age-related gains, as well as specific health life events and a higher awareness of age-related losses. Results furthermore indicated that the cumulative experience of family life events was associated with a higher awareness of age-related gains. Conversely, the cumulative experience of health events was associated with higher awareness of both age-related losses and age-related gains. Moreover, it was not only life events happening at an expected age, but also those happening relatively early and particularly those happening late in life, which were associated with AARC. In summary, life events and the change they may bring seem to be reflected in individuals' awareness of age-related losses and awareness of age-related gains.

Perceived personal deadlines for late-life preparation across adulthood.

Throughout adulthood, individuals follow personal timetables of deadlines that shape the course of aging. We examine 6-year-longitudinal data of perceived personal deadlines for starting with late-life preparation across adulthood. Findings are based on a sample of 518 adults between 18 and 88 years of age. Multilevel regression analyses were conducted to explore changes in personal deadlines for preparation in five domains (i.e., finances, end of life, housing, social connectedness, caregiving) in relation to calendar age, self-rated health, subjective position in life, and sociodemographic variables. Findings suggest that personal deadlines for starting preparatory activities differ depending on calendar age and domain of late-life preparation. Older adults as compared to younger adults are likely to report narrower deadlines for beginning with late-life preparation. Perceived deadlines for late-life preparation were furthermore found to be preponed and slightly dilated over time. Findings suggest that depending on age-graded opportunity structures, individuals flexibly adjust their personal deadlines for late-life preparation.

Worse or even better than expected? Outcome expectancies and behavioral experiences in the context of physical activity among cancer patients.

Expectancies of cancer patients regarding their physical activity before they took part in a behavior change intervention were compared with their experiences during the intervention period. A total of 66 cancer patients completed either a randomly assigned 4-week physical activity or a stress-management counseling intervention. On average, participants had positive expectancies toward physical activity. Outcome expectancies predicted outcomes (e.g. physical activity) at a 10-week follow-up. Outcome realization (discrepancy between expectancies and experiences) further increased explained variance in self-efficacy and physical activity enjoyment. In conclusion, not only initial outcome expectancies but also their realizations seem to be important for subsequent behavior and cognitions.

Daily experiences of subjective age discordance and well-being.

Subjective age discordance (SAD) captures the difference between how old one feels and how old one would ideally like to be. We investigated the presence, strength, and fluctuation of this discordance in daily life as well as its relationship to various indicators of physical and psychological well-being with an 8-day diary study. Participants were 116 older and 107 younger adults who completed daily measures of felt age, ideal age, positive and negative affect, physical symptoms, and stressors. We operationalized SAD as felt age minus ideal age divided by chronological age and compared the utility of this discordance to the more established proportional discrepancy of felt age from chronological age. Daily SAD was present in both age groups, such that individuals idealized younger ages than they felt. This discordance was larger in older than younger adults, although younger adults exhibited more daily fluctuations in SAD. Within-person increases in SAD were associated with lower positive affect, whereas larger SAD at the between-person level was associated with more physical symptoms and stressors. These relationships were over and above the associations of felt and chronological age with the outcomes suggesting the utility of daily SAD for understanding daily physical and psychological well-being. (PsycInfo Database Record (c) 2021 APA, all rights reserved).