ABSTRACT
Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range of applications due to poor performance under two-photon microscopy, a method of choice for deep-tissue recording. To improve indicators, we developed a multiparameter high-throughput platform to optimize voltage indicators for two-photon microscopy. Using this system, we identified JEDI-2P, an indicator that is faster, brighter, and more sensitive and photostable than its predecessors. We demonstrate that JEDI-2P can report light-evoked responses in axonal termini of Drosophila interneurons and the dendrites and somata of amacrine cells of isolated mouse retina. JEDI-2P can also optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 min using both resonant-scanning and ULoVE random-access microscopy. Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 µm and report voltage correlations in pairs of neurons.
Subject(s)
Microscopy , Neurons , Animals , Interneurons , Mice , Microscopy/methods , Neurons/physiology , Photons , WakefulnessABSTRACT
Guest-host Raman under liquid nitrogen spectroscopy (GHRUNS) is introduced whereby solid-state guest molecules are isolated inside cage-like host environments for the facile acquisition of their Raman spectra. This convenient method features reduced fluorescence, the analysis of populations in their ground states, and increased signal to noise ratios. Samples are also preserved through the reduction of thermal degradation and oxidation. To demonstrate the benefits of this new method, Raman spectra of the ubiquitous molecule C60 inside a cage of water ice are presented. Using this technique, a new normal mode of C60 is elucidated. The GHRUNS methodology is of interest to those seeking to acquire and characterize the vibrational spectra, structure, and properties of emissive, air-sensitive molecules.
Subject(s)
Quantum Theory , Vibration , Spectrum Analysis, Raman/methods , NitrogenABSTRACT
We experimentally demonstrate the interrogation of an individual Escherichia coli cell using a nanoscale plasmonic V-groove waveguide. Several different configurations were studied. The first involved the excitation of the cell in a liquid environment because it flows on top of the waveguide nanocoupler, while the obtained fluorescence is coupled into the waveguide and collected at the other nanocoupler. The other two configurations involved the positioning of the bacterium within the nanoscale waveguide and its excitation in a dry environment either directly from the top or through waveguide modes. This is achieved by taking advantage of the waveguide properties not only for light guiding but also as a mechanical tool for trapping the bacteria within the V-grooves. The obtained results are supported by a set of numerical simulations, shedding more light on the mechanism of excitation. This demonstration paves the way for the construction of an efficient bioplasmonic chip for diverse cell-based sensing applications.
Subject(s)
Biosensing Techniques/instrumentation , Escherichia coli/cytology , Lab-On-A-Chip Devices , Nanostructures/chemistry , Cells, Immobilized/chemistry , Cells, Immobilized/cytology , Computer Simulation , Equipment Design , Escherichia coli/chemistry , Fluorescence , Models, BiologicalABSTRACT
Next-generation optoelectronic devices and photonic circuitry will have to incorporate on-chip compatible nanolaser sources. Semiconductor nanowire lasers have emerged as strong candidates for integrated systems with applications ranging from ultrasensitive sensing to data communication technologies. Despite significant advances in their fundamental aspects, the integration within scalable photonic circuitry remains challenging. Here we report on the realization of hybrid photonic devices consisting of nanowire lasers integrated with wafer-scale lithographically designed V-groove plasmonic waveguides. We present experimental evidence of the lasing emission and coupling into the propagating modes of the V-grooves, enabling on-chip routing of coherent and subdiffraction confined light with room-temperature operation. Theoretical considerations suggest that the observed lasing is enabled by a waveguide hybrid photonic-plasmonic mode. This work represents a major advance toward the realization of application-oriented photonic circuits with integrated nanolaser sources.
ABSTRACT
An all-polymer photonic crystal slab sensor is presented, and shown to exhibit narrow resonant reflection with a FWHM of less than 1 nm and a sensitivity of 31 nm/RIU when sensing media with refractive indices around that of water. This results in a detection limit of 4.5 × 10(-6) RIU when measured in conjunction with a spectrometer of 12 pm/pixel resolution. The device is a two-layer structure, composed of a low refractive index polymer with a periodically modulated surface height, covered with a smooth upper-surface high refractive index inorganic-organic hybrid polymer modified with ZrO2based nanoparticles. Furthermore, it is fabricated using inexpensive vacuum-less techniques involving only UV nanoreplication and polymer spin-casting, and is thus well suited for single-use biological and refractive index sensing applications.
ABSTRACT
We demonstrate the highly efficient (>50%) conversion of freely propagating light to channel plasmon-polaritons (CPPs) in gold V-groove waveguides using compact 1.6 µm long waveguide-termination coupling mirrors. Our straightforward fabrication process, involving UV-lithography and crystallographic silicon etching, forms the coupling mirrors innately and ensures exceptional-quality, wafer-scale device production. We tailor the V-shaped profiles by thermal silicon oxidation in order to shift initially wedge-located modes downward into the V-grooves, resulting in well-confined CPPs suitable for nanophotonic applications.
ABSTRACT
A transparent, fully integrated electrically modulated projection technique is presented based on light guiding through a thin liquid crystal layer covering sub-wavelength gratings. The reported device operates at 10 V with response times of 4.5 ms. Analysis of the liquid crystal alignment shows that director-reorientation occurs over timescales on the order of 90 µs close to the grating surface. The technology is suitable for next generation heads-up-displays and reconfigurable multilayer photonic integrated circuits.
Subject(s)
Electronics/instrumentation , Fiber Optic Technology/instrumentation , Lighting/instrumentation , Liquid Crystals/chemistry , Refractometry/instrumentation , Electromagnetic Fields , Equipment Design , Equipment Failure Analysis , Liquid Crystals/radiation effectsABSTRACT
Enhanced control of diffraction through transparent substrates is achieved via disordered gratings in a silica sol-gel film. Tailoring the degree of disorder allows tuning of the diffractive behavior from discrete orders into broad distributions over large angular range. Gratings of optical quality are formed by silica sol-gel nanoimprint lithography and an optical setup for the measurement of continuous diffraction patterns is presented. Sound agreement is found between measurements and simulation, validating both the approach for redirection of light and the fabrication process. The disordered gratings are presented in the context of improved interior daylighting and may furthermore be suited to a wide variety of applications where controlled angular redirection of light is desired.
ABSTRACT
Interpreting chemical information and translating it into ethologically relevant output is a common challenge of olfactory systems across species. Are computations performed by olfactory circuits conserved across species to overcome these common challenges? To understand this, we compared odor responses in the locust antennal lobe (AL) and mouse olfactory bulb (OB). We found that odors activated nearly mutually exclusive neural ensembles during stimulus presentation ('ON response') and after stimulus termination ('OFF response'). Strikingly, ON and OFF responses evoked by a single odor were anticorrelated with each other. 'Inverted' OFF responses enhanced contrast between odors experienced close together in time. Notably, OFF responses persisted long after odor termination in both AL and OB networks, indicating a form of short-term memory. Taken together, our results reveal key neurodynamic features underlying olfactory computations that are conserved across insect and mammalian olfactory systems.
ABSTRACT
We demonstrate spectral filtering with state-of-the-art Bragg gratings in plasmonic V-groove waveguides fabricated by wafer scale processing based on nanoimprint lithography. Transmission spectra of the devices having 16 grating periods exhibit spectral rejection of the channel plasmon polaritons with 8.2 dB extinction ratio and -3 dB bandwidth of Δλ = 39.9 nm near telecommunications wavelengths. Near-field scanning optical microscopy measurements verify spectral reflection from the grating structures, and the oscillations of propagating modes along grating-less V-grooves correspond well with effective refractive index values calculated by finite element simulations in COMSOL. The results represent advancement towards the implementation of plasmonic V-grooves with greater functional complexity and mass-production compatibility.
Subject(s)
Molecular Imprinting/instrumentation , Nanostructures/chemistry , Photography/instrumentation , Refractometry/instrumentation , Surface Plasmon Resonance/instrumentation , Equipment Design , Equipment Failure Analysis , Nanostructures/ultrastructureABSTRACT
We present the enhanced transduction of a photonic crystal dye laser for gas sensing via deposition of an additional swelling polymer film. Device operation involves swelling of the polymer film during exposure to specific gases, leading to a change in total effective refractive index. Experimental results show an enhancement of 16.09 dB in sensing ethanol vapor after deposition of a polystyrene film. We verify different responses of the polystyrene film when exposed to either ethanol vapor or increased humidity, indicating selectivity. The concept is generic and, in principle, straightforward in its application to other intracavity-based detection schemes to enable gas sensing.
ABSTRACT
The main objective of a series of our researches is to develop a novel acoustic-based method for activation of biochar. This study investigates the capability of biochar in adsorbing Ni(II) as a hazardous contaminant and aims at enhancing its adsorption capacity by the addition of extra nitrogen and most probably phosphorous and oxygen containing sites using an ultrasono-chemical modification mechanism. To reach this objective, biochar physically modified by low-frequency ultrasound waves (USB) was chemically treated by phosphoric acid (H3PO4) and then functionalized by urea (CO(NH2)2). Cavitation induced by ultrasound waves exfoliates and breaks apart the regular shape of graphitic oxide layers of biochar, cleans smooth surfaces, and increases the porosity and permeability of biochar's carbonaceous structure. These phenomena synergistically combined with urea functionalization to attach the amine groups onto the biochar surface and remarkably increased the adsorption of Ni(II). It was found that the modified biochar could removeâ¯>â¯99% of 100â¯mg Ni(II)/L in only six hours, while the raw biochar removed only 73.5% of Ni(II) in twelve hours. It should be noted that physical treatment of biochar with ultrasound energy, which can be applied at room temperature for a very short duration, followed by chemical functionalization is an economical and efficient method of biochar modification compared with traditional methods, which are usually applied in a very severe temperature (>873â¯K) for a long duration. Such modified biochars can help protect human health from metal-ion corrosion of degrading piping in cities with aging infrastructure.
Subject(s)
Charcoal/chemistry , Environmental Pollutants/chemistry , Environmental Pollutants/isolation & purification , Nickel/chemistry , Nickel/isolation & purification , Ultrasonic Waves , Urea/chemistry , Adsorption , Graphite/chemistry , Phosphoric Acids/chemistryABSTRACT
We demonstrate a direct, single measurement technique for characterizing the dispersion of a photonic crystal waveguide (PCWG) using a tapered fiber evanescent coupling method. A highly curved fiber taper is used to probe the Fabry-Pérot spectrum of a closed PCWG over a broad k-space range, and from this measurement the dispersive properties of the waveguide can be found. Waveguide propagation losses can also be estimated from measurements of closed waveguides with different lengths. The validity of this method is demonstrated by comparing the results obtained on a 'W1' PCWG in chalcogenide glass with numerical simulation.
Subject(s)
Algorithms , Equipment Failure Analysis/instrumentation , Equipment Failure Analysis/methods , Fiber Optic Technology/instrumentation , Equipment DesignABSTRACT
We demonstrate the spectral and spatial reconfigurability of photonic crystal double-heterostructure cavities in silicon by microfluidic infiltration of selected air holes. The lengths of the microfluidic cavities are changed by adjusting the region of infiltrated holes in steps of several microns. We systematically investigate the spectral signature of these cavities, showing high Q-factor resonances for a broad range of cavity lengths. The fluid can be removed by immersing the device in toluene, offering complete reconfigurability. Our cavity writing technique allows for tolerances in the infiltration process and provides flexibility as it can be employed at any time after photonic crystal fabrication.
Subject(s)
Crystallization/instrumentation , Microfluidics/instrumentation , Optics and Photonics/instrumentation , Crystallization/methods , Equipment Design/instrumentation , Microfluidic Analytical Techniques/instrumentation , Nanotechnology/instrumentation , Nanotechnology/methods , Photons , Spectrophotometry/methods , TransducersABSTRACT
We demonstrate highly efficient evanescent coupling via a silica loop-nanowire, to ultra-small (0.5 (lambda/n)(3) ), InAs/InP quantum dot photonic crystal cavities, specifically designed for single photon source applications. This coupling technique enables the tuning of both the Q-factor and the wavelength of the cavity mode independently, which is highly relevant for single photon source applications. First, this allows for the optimization of the extraction efficiency while maintaining a high Purcell factor. Second, the cavity mode can be matched with a spectrally misaligned quantum dot without changing the structure or degrading the Q-factor: a 3 nm resonance shift is reported.
ABSTRACT
We present experimental results on post-tuning the dispersion of a two-dimensional photonic crystal waveguide made from Ge(33)As(12)Se(55) chalcogenide glass by exploiting the material photosensitivity to near-bandgap light. The change in the refractive index and volume of the material in response to exposure to 633nm light resulted in a shift of more than 5nm in the resonant coupling wavelength between a tapered optical fiber and the modes of a W1 waveguide. This represents a first proof of principle demonstration of the photosensitive post-tuning of a planar photonic crystal device.
ABSTRACT
The ability to handle single, free molecules in lab-on-a-chip systems is key to the development of advanced biotechnologies. Entropic confinement offers passive control of polymers in nanofluidic systems by locally asserting a molecule's number of available conformation states through structured landscapes. Separately, a range of plasmonic configurations have demonstrated active manipulation of nano-objects by harnessing concentrated electric fields. The integration of these two independent techniques promises a range of sophisticated and complementary functions to handle, for example, DNA, but numerous difficulties, in particular, conflicting requirements of channel size, have prevented progress. Here, we show that metallic V-groove waveguides, embedded in fluidic nanoslits, form entropic potentials that trap and guide DNA molecules over well-defined routes while simultaneously promoting photothermal transport of DNA through the losses of plasmonic modes. The propulsive forces, assisted by in-coupling to propagating channel plasmon polaritons, extend along the V-grooves with a directed motion up to ≈0.5 µm·mW-1 away from the input beam and λ-DNA velocities reaching ≈0.2 µm·s-1·mW-1. The entropic trapping enables the V-grooves to be flexibly loaded and unloaded with DNA by variation of transverse fluid flow, a process that is selective to biopolymers versus fixed-shape objects and also allows the technique to address the challenges of nanoscale interaction volumes. Our self-aligning, light-driven actuator provides a convenient platform to filter, route, and manipulate individual molecules and may be realized wholly by wafer-scale fabrication suitable for parallelized investigation.
Subject(s)
Equipment Design/methods , Nanotechnology/methods , Biopolymers/chemistry , DNA/classification , Entropy , Light Signal Transduction , Molecular Conformation , Polymers/chemistryABSTRACT
Lasing emission from random cavities formed in networks of electrospun Rhodamine-doped polymer fibers is presented. Spatially resolved spectroscopy and spectral analysis prove that the observed laser emission stems from individual ring resonators randomly distributed throughout the network. These electrospun fiber lasers represent a facile and straightforward configuration for developing novel photonic devices that may advantageously utilize the network morphology.
Subject(s)
Polymers/chemistry , Lasers , Microscopy, Electron, Scanning , Polymethyl Methacrylate/chemistry , Rhodamines/chemistryABSTRACT
We demonstrate postprocessed microfluidic double-heterostructure cavities in silicon-based photonic crystal slab waveguides. The cavity structure is realized by selective fluid infiltration of air holes using a glass microtip, resulting in a local change of the average refractive index of the photonic crystal. The microcavities are probed by evanescent coupling from a silica nanowire. An intrinsic quality factor of 57,000 has been derived from our measurements, representing what we believe to be the largest value observed in microfluidic photonic crystal cavities to date.
ABSTRACT
We report detailed measurements of the optical properties of tapered photonic crystal fibers (PCFs). We observe a striking long-wavelength loss as the fiber diameter is reduced, despite the minimal airhole collapse along the taper. We associate this loss with a transition of the fundamental core mode as the fiber dimensions contract: At wavelengths shorter than this transition wavelength, the core mode is strongly confined in the fiber microstructure, whereas at longer wavelengths the mode expands beyond the microstructure and couples out to higher-order modes. These experimental results are discussed in the context of the so-called fundamental mode cutoff described by Kuhlmey et al. [Opt. Express 10, 1285 (2002)], which apply to PCFs with a finite microstructure.