ABSTRACT
Three new methods were developed for the quantitative determination of mesalazine in the form of the pure substance or in the form of suppositories and tablets - accordingly: bromatometric, diazotization and visible light spectrophotometry method. Optimizing the time and the temperature of the bromination reaction (50°C, 50 min) 4-amino-2,3,5,6-tetrabromophenol was obtained. The results obtained were reproducible, accurate and precise. Developed methods were compared to the pharmacopoeial approach - alkalimetry in an aqueous medium. The validation parameters of all methods were comparable. Developed methods for quantification of mesalazine are a viable alternative to other more expensive approaches.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/analysis , Bromine Compounds/analysis , Diazonium Compounds/analysis , Mesalamine/analysis , Spectrophotometry/methods , Anti-Inflammatory Agents, Non-Steroidal/chemistry , Bromine Compounds/chemistry , Chemistry, Pharmaceutical , Diazonium Compounds/chemistry , Drug Compounding , Kinetics , Mesalamine/chemistry , Reproducibility of Results , Suppositories , Tablets , TemperatureABSTRACT
A rapid, convenient, precise HPLC method was developed for the simultaneous determination of aripiprazole and its active metabolite dehydroaripiprazole in blood serum. The separation was carried out by RP HPLC on Symmetry C18 column (150 x 4.6 mm; 5 µm). The mobile phase was composed of acetonitrile: water (30 : 70, v/v), pH 3.0 adjusted with o-phosphoric acid. The detection was monitored at 220 nm.