[Mechanism of the modifying effect of neuraminidase of the influenza virus on the development of Rauscher leukemia]. / Mekhanizm modifitsiruiushchego vliianiia neraminidazy virusa grippa na razvitie leikoza Raushera.

It was established that neuraminidase--an immunosuppressive component of influenza virus--stimulated Rauscher leukemia in BALB/c mice. This effect is connected with earlier manifestation of sensitization to embryonic antigens and the appearance of immature PNL+ cells in spleens of infected mice rather than with immunosuppression. The mechanism of cocarcinogenic effect of viral neuraminidase is discussed.

[Analytical microelectrophoresis of cells and the means for using it in oncological research]. / Analiticheskii mikroélektroforez kletok i puti ego ispol'zovaniia v onklologicheskikh issledovaniiakh.

Analysis of data on the study of electrophoretic cell mobility in the field of oncology is reviewed. Much attention is paid to the application of cell surface phenomena for the development of different diagnostic methods, especially for immunological diagnosis of cancer.

[Reproduction and assembly of oncoviruses]. / Reproduktsiia i sborka onkovirusov.

Modern investigations of structural and functional organization of oncoviruses are analyzed. The data available in the literature on oncovirus interaction with host cell membrane (adsorption, penetration by endocytosis and fusion, uncoating) as well as the cell and viral receptors are elucidated. The results of the studies on retroviral organization of genome, its integration with cellular genome, transcription and translation are given. The functions of long terminal repeats are described. The characteristic of the end step of life cycle of retroviruses (assembly, budding and maturation) is presented.

[Development of lung tumors in BALB/c mice in chronic influenza infection and inhalation of metal-containing aerosols]. / Vozniknovenie opukholei legkikh u myshei BALB/c pri khronicheskoi grippoznoi infektsii i ingaliatsii metallosoderzhashchego aérozolia.

Intensification of cocarcinogenic effect of chronic influenza infection (10 months after virus instillation) in BALB/c mice was obtained by polymetallic aerosol inhalation 5 or 6 months after infection. An increase of malignant neoplasms incidence was accompanied by a decrease of the cytoproliferative activity in the thymus and spleen. The immunopathogenic and cocarcinogenic effects were maximal when the aerosol treatment was carried out 6 months after infection.

[Isolation and characteristics of the fibroblast cell line of rats cotransformed by the DNA of simian adenovirus SA7 (C8) and hepatitis B virus]. / Poluchenie i kharakteristika linii kletok fibroblastov krysy, kotransformirovannykh DNK adenovirusa obez'ian SA7 (C8) i virusa gepatita B.

Transformed cell line (SH2) was established by means of co-transfection of primary rat embryonal fibroblasts by DNA of high-oncogenic simian adenovirus SA7 (C-8) and hepatitis B virus. SH2 cells possess a transformed phenotype and high oncogenicity both for allogenic (rats) and xenogenic (hamsters) animals. 100 SH2 cells induce tumours in newborn (3 day) hamsters. 10(4) SH2 cells inoculated to adult hamsters induce tumours in approximately 50% cases on the 20th-30th day. Possible mechanisms of significant stimulation of SH2 cells oncogenic properties after co-transfection by DNA of oncogenic adenovirus SA7 and hepatitis B virus are discussed.

[Preparation and characteristics of a cell line from transplantable Svec's leukemia]. / Poluchenie i kharakteristika kletochnoi linii iz transplantiruemogo leikoza Shvetsa.

The procedure for obtaining the CL-C5 suspension cell line from the transplantable rat leukemia (erythromyelosis) is described. Cytochemical and biochemical features of CL-C5 blast cells are established to be typical of T-cells at the differentiation stage, corresponding to thymocytes. The results obtained permit considering CL-C5 cell line as that having phenotypical indications of lymphocytes and retrovirus production lost due to the selection in the cell population.

[Enhancement of the transforming activity of simian adenovirus S5 and of the DNA from human adenovirus type I by benzo(a)pyrene and TPA]. / Usilenie transformiruiushchei aktivnosti adenovirusa S5 obez'ian i DNK adenovirusa tipa I cheloveka benz(a)pirenom i TFA.

Benz(a)pyrene in a concentration of 2.5 micrograms/ml produced a 3.3-fold increase in the transforming activity of simian adenovirus S5 in primary cultures of rat kidney cells. Under analogous conditions TPA increased (2.1 times) the transforming activity of DNA of human adenovirus of type I (AdI). PX5 cells (cotransformed by S5 and benz(a)pyrene) induced tumours in 100% of syngeneic and 81% xenogeneic animals. PX4 cells (transformed by S5 only) induced tumours in 60% of syngeneic animals. Cells transformed by DNA AdI and DNA AdI + TPA did not induce tumours in animals.

Effect of influenza virus on the immune responsiveness of animals.

Infection of mice with A/Hong Kong/1/68 (H3N2) and A/PR8/34 (H0N1) influenza virus strains resulted in a significant inhibition of the formation of antibody-producing cells in response to administration of sheep erythrocytes and a reduced capacity of spleen cells to induce "graft-versus-host" reaction. The immunosuppression caused by influenza infection was observed for a long period of time post infection (3--6 months). The extent of inhibition of antibody production depended on the dose of virus, route of inoculation, the sequence of infection and immunization and the internal between them. Heat-inactivated virus exerted no immunosuppressive effect.

[Secondary intensification of influenza virus reproduction and the development of tumors in mice]. / Vtorichnoe usilenie reproduktsii virusa grippa i razvitie opukholei legkikh u myshei.

Tumor disposition in mice with chronic influenza infection was shown to be completed chiefly during secondary increase in viral reproduction. This condition presents with various systemic disorders and a pronounced increase in susceptibility to the cocarcinogenic effect of polymetal aerosols.

[Effect of the influenza virus and its structural components on the animal immunocompetence system]. / Vliianie virusa grippa i ego strukturnykh komponentov na immunokompetentnuiu sistemu zhivotnykh.

The authors studied the effect of the influenza virus A (PR8/34) and of its structural components on the immunological reactivity of mice. The enzyme of the external coat of the influenza virus--neuraminidase--possessed an immunodepressive action. Administration of neuraminidase led to the elimination of sialic acids from the surface of lymphocytes and to the reduction of their electrophoretic mobility. The mechanism of the immunodepressive action of neuraminidase is discussed.