Effect of aerobic exercise on insulin, insulin-like growth factor-1 and insulin-like growth factor binding protein-3 in overweight and obese postmenopausal women.

AIM: The purpose of presented study was to verify the influence of aerobic physical activity program on the serum insulin, insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) concentrations in overweight and obese postmenopausal women. METHODS: Somatic parameters and serum concentrations of insulin, IGF-1 and IGFBP-3 were measured in 21 overweight and obese women, before and after 8-week cycloergometer physical workout and 16 age-matched, controls. Age of all studied women ranged from 54 to 78 years. RESULTS: The comparative analysis of biochemical indices measured before and after the training program showed that the systematic exercise cause the significant decrease in insulin (P<0.05) and IGF-1 concentrations (P<0.05). There were no differences in these parameters in controls over the studied period. The level of IGFBP-3 was not significantly changed in both investigated groups. In women participating in the training program the positive correlation (P<0.05) between changes in IGF-1 levels (Δ) and changes in insulin concentrations (Δ) within two terms of the study were found. The magnitude of changes (Δ) in insulin and IGF-1 levels over the study period correlated with their concentrations measured before the training program (P<0.01). CONCLUSION: Results of the present study indicate that regular aerobic physical activity decreases serum insulin and IGF-1 levels in postmenopausal women. The magnitude of insulin and IGF-1 concentration changes depend on their initial levels. Changes of IGF-1 levels are associated with insulin concentration modifications.

Visfatin, resistin, hsCRP and insulin resistance in relation to abdominal obesity in women with rheumatoid arthritis.

OBJECTIVES: The increasing incidence of overweight and obesity in modern societies, and the demonstration that adipose tissue produces numerous cytokines, increases interest of investigators in the associations between adipose tissue, adipokines, CRP and insulin resistance in rheumatoid arthritis (RA) and their possible role in the pathogenesis of this disease.The purpose of the study was to investigate serum levels of visfatin, resistin, hsCRP and insulin resistance in relation to abdominal obesity in patients with RA. METHODS: Serum levels of visfatin, resistin, hsCRP and glucose metabolism parameters were measured in 46 women with RA - 22 without abdominal obesity and 24 with abdominal obesity. RESULTS: Patients with RA and abdominal obesity showed significant lower visfatin levels (1.00+/-0.93 vs. 1.44+/-0.85 ng/ml; p<0.05) in comparison to those without abdominal obesity. We found significant correlations between levels of visfatin and resistin (r=0.41; p<0.01); and between resistin levels and hsCRP levels (r=0.41; p<0.01); resistin levels and leucocytes count (r=0.36; p<0.05); and resistin levels and ESR (r=0.30; p<0.05), in the whole investigated group. Visfatin as well as resistin did not correlate with anthropometric parameters or insulin resistance in whole patients with RA. CONCLUSIONS: In patients with RA and abdominal obesity, in comparison to patients without abdominal obesity, significantly lower visfatin levels have been demonstrated. Resistin was associated with laboratory markers of inflammation. Positive correlation between levels of visfatin and resistin may suggest that visfatin plays a role in inflammation in RA.

Insulin resistance and glucose tolerance in obese women: the effects of a recreational training program.

AIM: This study was designed to investigate whether recreational physical activity based on aquatic training may improve lipid profile and glucose tolerance in obese subjects. Additionally, we intended to assess a possible correlation between insulin resistance index HOMA(IR) with another index including serum adiponectin concentrations. METHODS: Study population consisted of 12 obese women aged 44-61, who volunteered for an aquatic training 1 h twice a week for 3 months. Before entering the training program, and after completing it patients' oral glucose tolerance test was performed. Glucose, insulin and adiponectin concentrations, levels of antibodies to oxidatively modified low-density lipoproteins (oLAB), and lipid profiles were measured in blood samples collected before the oral glucose tolerance test (OGTT). Glucose and insulin concentrations were also assessed in blood samples collected at 30 and 120 min of OGTT. Atherogenic index of plasma (AIP), and insulin sensitivity indexes HOMA(IR) and HOMA(AD) were calculated on the basis of the obtained data. RESULTS: The 3-month, recreational aquatic training had no influence on body mass, but it resulted in improved glucose tolerance (at t = 0 and t = 120 min), decreased index (HOMA(IR)), and decreased levels of total and LDL-cholesterol (P<0.05). Adiponectin concentrations and values of HOMA(AD) remained unchanged. We found a significant correlation between adiponectin levels and concentrations of total and LDL cholesterol, and between insulin indexes HOMA(IR) and HOMA(AD) both before, and after the training (P<0.01). CONCLUSION: The results of our study indicate that a 3-month recreational training consisting in water aerobic results in favorable changes in glucose and lipid metabolism in obese subjects, even despite the lack of improvement in body mass.

Beneficial effect of short-term endurance training on glucose metabolism during rehabilitation after coronary bypass surgery.

AIMS: Our study was aimed at determining whether beneficial modification of carbohydrate metabolism can be obtained after a short-term training program and whether it is associated with an increase in binding and degradation of (125)I-insulin by erythrocyte receptors that suggests a decrease in insulin resistance. METHODS: The study was conducted in a group of 20 patients aged 56 +/- 1.9 years (mean +/- SEM), within 1 to 6 months after coronary bypass surgery. All patients completed 15 training sessions based on 30 min of cycling with a constant load. Before and after a 3-week training program, glucose, insulin, and C-peptide blood levels, as well as binding and degradation of (125)I-insulin by erythrocyte receptors, were determined. RESULTS: A statistically significant decrease was found in the blood glucose level, from 111.2 +/- 4.2 to 97.8 +/- 3.5 mg/dL (p < 0.01); this decrease was not accompanied by significant insulin concentration changes. There was also a significant increase in insulin binding, from 0.535 +/- 0.059 to 0.668 +/- 0.042 pg (125)I/10(11) RBCs (p < 0.01), and degradation from 7.64 +/- 0.54 to 9.49 +/- 0.58 pg (125)I/10(11) RBCs (p < 0.05). CONCLUSION: The results indicated that even short-term endurance training in patients rehabilitated after coronary bypass surgery induced favorable modification of glucose metabolism, presumably caused by a decrease in insulin resistance.

Exercise-induced increase in hydrogen peroxide plasma levels is diminished by endurance training after myocardial infarction.

We have investigated the influence of physical training on exercise-induced changes in free radical activity in patients after myocardial infarction. Seventeen patients admitted to the cardiac rehabilitation center performed a bicycle ergometry before and after a 3-week endurance training program. The oxygen consumption and carbon dioxide production were measured and the ECG and the systemic blood pressure were monitored during the tests. Blood samples for acid-base equilibrium, lactic acid levels and hydrogen peroxide concentrations were collected directly before and after each exercise test. Exercise-induced increases in systolic blood pressure, heart rate and rate-pressure product as well as lactic acid concentrations were significantly less marked during the bicycle ergometry performed after the rehabilitation program. During the initial exercise test hydrogen peroxide levels increased significantly from 7.15+/-0.74 micromol/l before to 9.09+/-1.04 micromol/l 3 min after the test (P=0.0229). In contrast, no significant changes in hydrogen peroxide concentrations were observed during the exercise test performed after the training (6.31+/-1.05 micromol/l before the test, 5.85+/-1.08 micromol/l after the test, P=0.201). These observations suggest that physical training may have a beneficial influence on free radicals' generation in patients after myocardial infarction.

Result of oral intake of glucose by healthy subjects and patients with essential hypertension on the binding and degradation of 125I-insulin by erythrocyte receptors.

The work presents the results of researches of binding and degradation of 125I-Insulin by erythrocyte receptors in the patients with essential hypertension and healthy patients after glucose intake. In order to obtain full representation of the pattern of changes the serum IRI and glucose concentrations were assayed. Binding and degradation of 125I-Insulin by erythrocyte receptors were determined with the method described by Gambhir (1977), modified by the authors. The modification consisted in usage of constant concentrations of iodized insulin (0.9 pg/0.1 ml) and bovine insulin (2.4 I.U./0.1 ml). Before administration of glucose and in 30, 60 and 120 minutes after, venous blood was collected from ulnar vein. All examined persons were in sitting position during the trial of glucose intake. Obtained results show, that blood insulin level in the patients with essential hypertension is statistically significantly higher than in healthy persons of similar anthropometric characteristics. Binding of 125I-Insulin to erythrocyte receptors in fasting state is statistically significantly lower. Degradation after glucose intake in the patients shows decreasing tendency, while in healthy persons--growing tendency.

Glucosamine in serum of patients after myocardial infarction subjected to rehabilitation training.

This paper presents results of 3 weeks physical training on glucosamine level in serum of male patients after myocardial infarction (MI) aged between 38 and 61. Patients were randomised in two groups: the training group (n = 21), staying in Cardiac Rehabilitation Department and the control group (n = 11), discharged home for 3 weeks. Each group received identical dietary instructions. The training group performed exercises every day: on bicycle ergometer during 30 minutes (5 times a week), overall-conditioning exercises for 30 minutes daily and 30 to 60 minutes of walking each day. Before administering of the therapy and 3 weeks later all MI patients performed the bicycle ergometer exercise test until the ventilatory threshold was reached. Before that test and 3 minutes after its termination capillary and venous blood samples were drawn. In the capillary blood samples indices of acid-base balance, lactate level, and glucose level were determined. In venous blood samples the serum levels of immunoreactive insulin, C-peptide and glucosamine were determined as well as binding of 125I-insulin to erythrocyte receptors. Obtained results show that administered therapy increased physical fitness and decreased of glucosamine concentration, insulinaemia and insulin resistance.

The influence of exercise-induced oxidative stress on binding and degradation of 125I-insulin by the receptors on erythrocytes.

In the present work we investigated the influence of oxidative stress induced by strenuous exercise on the affinity of a specific insulin receptors on human erythrocytes. 13 male members of the national basketball staff performed a maximal treadmill exercise test. In the blood samples collected before and directly after the test acid-base equilibrium parameters, lactic acid concentrations, glucose and insulin serum levels as well as 125I-insulin binding and degradation by receptor on erythrocytes were measured. As markers of oxidative stress, plasma thiobarbituric acid-reactive substance levels (TBARS) and red blood cells glutathione content (GSH) were determined. After the exercise test TBARS levels increased significantly and GSH concentrations decreased indicating that oxidative stress occurred. Binding of 125I-insulin to the receptors on erythrocytes decreased significantly during the test, while there was only insignificant reduction in 125I-insulin degradation. Correlation analysis and multiple linear regression revealed that changes in insulin degradation by receptors on erythrocytes during exhaustive exercise are determined by oxidative stress, probably via oxidation of sulfhydryl groups of certain enzymes. The affinity of receptors for insulin seems to depend mainly on glucose concentrations.

The influence of oral glucose intake on binding and degradation of 125I-insulin by receptors on erythrocytes as well as on insulin and C-peptide serum levels in patients after myocardial infarction and healthy individuals.

In this study, we investigated the influence of glucose administration on binding and degradation of 125I-insulin by receptors on erythrocytes as well as on insulin and C-peptide serum levels in 15 patients after myocardial infarction and in 15 age-matched healthy persons. Venous blood samples were taken directly before and at 30, 60 and 120 minutes after oral administration of 75 g of glucose. In the collected blood samples serum glucose, insulin and C-peptide levels were determined. Binding and degradation of 125I-insulin by specific receptors on red blood cells were evaluated using the method described by Gambhir and modified by the authors. Serum insulin and C-peptide levels were significantly higher while binding of 125I-insulin to erythrocytes was decreased in patients after myocardial infarction. These results seem to support the hypothesis that insulin resistance and hyperinsulinism play a role in the pathogenesis of ischaemic heart disease. Impaired degradation of 125I-insulin during the oral glucose tolerance test in the patients after myocardial infarction indicates that insulin resistance is located at the receptor level.

[Glucosamine in the blood serum of young people with diabetes mellitus type 1]. / Glukozamina w surowicy krwi mlodziezy z cukrzyca typu 1.

The observations of some authors have shown that prolonged hyperglycemia may increase the process of hexosamin biosynthesis. The purpose of our study was to determine glucosamine levels in young patients with poorly controlled diabetes mellitus type 1. The study group consisted of 31 girls and boys whose ages ranged from 7 to 17 and who were newly diagnosed with type 1 diabetes mellitus. The diagnosis of diabetes mellitus was based on increased anti-insulin antibody levels, raised glycated hemoglobin and glucose concentrations and decreased insulin and C-peptide levels. In the studied group impairments in acid-base homeostasis as well as a correlation between the degree of blood acidification and free fatty acids, total cholesterol, and triglyceride levels were found. Despite evident hyperglycemia observed in all patients, glucosamine levels were within the normal range. Based on the present and previous investigations, where we observed increased hexosamine levels only in patients with hyperinsulinemia, we can draw the conclusion that increased enzymatic glycation of proteins requires not only increased glucose but also insulin access.

Resistin, visfatin and insulin sensitivity in selected phases of annual training cycle of triathletes.

The purpose of the study was to examine the effects of sport training on carbohydrate metabolic indices and adipokines concentrations in young male triathletes (n=10). Athletes performed the incremental running test in two periods of the training cycle: in the transitory and preparatory phases. In both analyzed terms, physical exercise was reflected by a significant increase in lactate (p≤0.01), insulin (p≤0.01), visfatin concentrations (p≤0.01, p<0.05, respectively) and only during transitory phase in glucose (p≤0.01) and resistin concentrations (p<0.05). Significant inter-period differences were noted in the pre-exercise insulin (p≤0.01) and also in pre- and post-exercise visfatin concentrations (p<0.05). Additionally, the differences (Δ) between post- and pre-exercise values of glucose (p<0.05) and visfatin (p≤0.01) significantly decreased in the preparatory phase comparing to the transitory phase. The inverse correlations between pre-exercise concentrations of visfatin and peak oxygen uptake (p<0.05) in the transitory phase and between post- and pre-exercise differences (Δ) of visfatin and lactate concentrations (p<0.05) in the preparatory phase were noted. During preparatory phase, pre-exercise visfatin concentrations inversely correlated with pre-exercise resistin, insulin and glucose levels (p<0.05). In conclusion, systematic training in elite triathletes modulates basal adipokine concentrations only to a small extent, however, influences on these molecules response on the acute exercise.

New 2-methylimidazole-dicarboxylic acid molecular crystals: crystal structure and proton conductivity.

Three new proton conducting molecular crystals, 2-methylimidazole glutarate, 2-methylimidazole suberate and 2-methylimidazole azelate, were obtained and their structure was determined by the x-ray diffraction method. The structure of the crystals was found to be of layer-type. A hydrogen bond network between the heterocycle, glutaric acid and water molecules was apparent in a single layer of 2-methylimidazole glutarate, whereas chains consisting of two heterocyclic molecules linked with hydrogen bonds with dicarboxylic acid were distinguished in a single layer of 2-methylimidazole suberate and azelate crystals. Thermal stability of the crystals was characterized by differential scanning calorimetry and the electrical conductivity was studied by the impedance spectroscopy method. The maximum conductivity of 2-methylimidazole glutarate pellets amounts to 3.3 × 10(-2) S m(-1) at 325 K, in the case of 2-methylimidazole suberate pellets the maximum conductivity is 2.4 × 10(-4) S m(-1) at 348 K and for 2-methylimidazole azelate pellets the maximum conductivity reaches 6.9 × 10(-4) S m(-1) at 353 K.

Biochemical markers of bone metabolism in healthy elderly men. The relationship to physical activity.

The purpose of the present study was to examine the serum levels of bone turnover markers and interleukin 1beta in healthy elderly men with different levels of habitual physical activity. The study was conducted on 40 healthy men aged between 65 and 85 years (mean 74.0 +/- 7.55 years). The study population was divided into two groups with respect to the level of physical activity: 22 less active subjects (energy expenditure related to physical activity below 1600 kcal per week) and 18 more active subjects (above 1600 kcal per week). To estimate the physical activity of the study population, weekly energy expenditure was measured in each subject with use of Caltrac accelerometer. In the blood serum levels of bone formation marker--osteocalcin and resorption marker--the C-terminal telopeptide of collagen I (ICTP), and interleukin-1beta were determined. Concentrations of osteocalcin and ICTP in less active subjects were significantly higher (by 3.62 ng/ml and by 1.21 mug/l respectively, p < 0.05), reflecting the higher bone turnover in comparison to values determined in more active subjects. Positive correlations between ICTP and osteocalcin levels (r = 0.5814 and r = 0.6526; p < 0.05), and between ICTP and IL-1beta (r = 0.5823 and r = 0.6040; p < 0.05) in less and more active men were found. On the ground of the presented study it can be concluded that the decreased bone turnover occurred in men with higher level of habitual physical activity. This study showed the relationship between serum level of IL-1beta and bone resorption but the habitual physical activity has no effect on this cytokine activity.

[Fructosamine in blood serum, binding and degradation of 125J-insulin by erythrocyte receptors in young persons with type I diabetes--effect of physical exercise]. / Fruktozamina w surowicy krwi oraz wiazanie i degradacja 125J-insuliny przez receptory erytrocytów u mlodych osób z cukrzyca I typu--wplyw wysilku fizycznego.

The aim of investigation was the determination of the effect of regular physical exercise of intensity 35% VO2max on glycolysation of proteins, expressed by fructosamine concentration in blood serum and on insulin sensitivity of erythrocyte receptors in children with diabetes mellitus type I. The investigations were performed with 10 young persons with diabetes mellitus type I, during their sanatorium treatment. During 21 days the children effected every day a 20-minutes ergometric exercise of intensity equivalent approximately to 35% VO2max. Before the 3-weeks therapy and after its termination the examined children have performed an ergometric test exercise, with collection of blood samples. Obtained results allow to ascertain, that regular aerobic exercise contributed to the growth of physical efficiency expressed by the VO2max value, reduction in fructosamine level in blood serum, increase in insulin sensitivity of erythrocyte receptor and improved effort tolerance related to glycemia.

The influence of short-term endurance training on the insulin blood level, binding, and degradation of 125I-insulin by erythrocyte receptors in patients after myocardial infarction.

PURPOSE: This study was directed toward establishing whether and to what extent, short-term endurance training influences the insulin blood level, and the binding and degradation of 125I-insulin by erythrocyte receptors in patients undergoing rehabilitation after myocardial infarction. METHODS: The study was conducted in a group of 60 patients who had had myocardial infarction within the past 1.5 to 3 months and who did not have arterial hypertension and diabetes mellitus. All the patients took a symptom-limited cardiopulmonary exercise test. Before and after the test, venous blood was collected to determine lactic acid and insulin blood levels as well as the binding and degradation of 125I-insulin. The study group was randomized into two subgroups. One subgroup entered into a 3-week in-patient rehabilitation course. The control group was discharged from the hospital and was given no recommendations for physical exercise. The same investigation was repeated 3 weeks later. RESULTS: In the patients (50%) with hyperinsulinemia (insulin resistance index, > 10 microIU/mL), which was detected during the first investigation, insulin blood level decreased from 23.9 +/- 4.4 to 15.0 +/- 1.9 microIU/mL (P < 0.05) after rehabilitation, whereas insulin binding increased from 0.67 +/- 0.05 to 0.85 +/- 0.08 pg 125I/10(11) erythrocytes (P < 0.05). In the control group, which included normal subjects and those with hyperinsulinemia, the results obtained during the first and second investigations showed no statistically significant changes when compared. CONCLUSIONS: The results suggest that a 3-week endurance training period during rehabilitation after myocardial infarction reduces insulin resistance in patients with hyperinsulinemia.

Glucosamine levels in people with ischaemic heart disease with and without type II diabetes.

Glucosamine has a major influence on the impairment of some metabolic mechanisms in the human body. As shown in vitro experiments, it takes part in inducing mechanisms of insulin resistance. Therefore, the purpose of our study was to evaluate glucosamine levels in the serum of patients who suffered myocardial infarction (MI) and who either had or didn't have diagnosed type II diabetes in relation to healthy people. The levels of glucosamine, immunoreactive insulin, C-peptide, glucose and lipid indexes were measured in venous blood in investigated patients. In patients with MI without diabetes the highest concentrations of glucosamine, insulin and C-peptide were noted as compared to the results obtained from other groups of patients. In patients with diabetes, on the other hand, the highest glucose levels were noted as compared to the results of other patients. There were no statistically differences of lipid indexes between two groups of patients following MI. A negative correlation between glucosamine levels and glucose concentrations in patients without diabetes may suggest that glucose does not directly determine glucosamine levels. The returning of insulin levels to normal in patients with hyperinsulinemia (antidiabetic drugs) may play a role in the lowering of glucosamine induced peripheral insulin resistance.

[Insulin binding to erythrocyte receptors in patients with essential hypertension]. / Wiazanie insuliny przez receptory insulinowe erytrocytów chorych na nadcisnienie tetnicze pierwotne.

Recently many researchers have described the presence of insulin resistance and hyperinsulinemia in a substantial number of patients with essential hypertension. Reduced insulin binding to the receptors may play important role in development of insulin resistance in these patients. The study was aimed to assess the value of insulin binding to erythrocyte receptors in the patients with essential hypertension and compare to values in healthy persons. Additional purpose was the evaluation of insulin degradation by erythrocytes in patients with essential hypertension. 23 patients with essential hypertension (BMI 22.7 +/- 3.2) and 21 healthy persons (with BMI value 23.3 +/- 2.9) were studied. In all examined individuals the blood glucose and blood insulin concentrations were determined, insulin binding to erythrocyte receptors and insulin degradation by erythrocytes were measured by the method of Gambhir and al. Insulin concentration was significantly higher in patients with essential hypertension than in healthy subjects. We demonstrated a statistically significant positive correlation between body weight and insulin concentration in blood serum only in healthy people. Insulin binding to the receptors of red blood cells was significantly stronger (p < 0.001) in healthy persons than in patients with essential hypertension (0.972 +/- 0.395 pg 10(11) RBC and 0.446 +/- 0.14 pg 10(11) RBC respectively). In patients with hypertension insulin binding to receptors of red blood cells does not depend on body weight and insulin concentration in blood serum. Values of insulin degradation by erythrocyte in patients with essential hypertension and healthy persons were not significantly different. It seems that decreased insulin binding to insulin receptors is an important mechanism of insulin resistance patients with essential hypertension.

Oxidative stress and antioxidant defense system in healthy, elderly men: relationship to physical activity.

The aim of the present study was to compare selected markers of oxidative stress and the antioxidant system between groups of men aged 65 or more, differentiated by their level of physical activity. Thirty-one community-dwelling healthy, elderly men (mean age 73.4 +/- 6.1 years) with a good nutritional status participated in the study. The Caltrac accelerometer was used to estimate energy expenditure related to physical activity. Subjects were divided into two groups according to the criteria proposed by Bauman: less active (< 1600 kcal/week) and more active (> 1600 kcal/week). In all the men, plasma total antioxidant status (TAS), thiobarbituric acid reactive substances (TBARS) concentrations and hydrogen peroxide (H2O2) levels were determined. The reduced glutathione (GSH) content and glutathione peroxidase (GPx) activity in hemolyzed red blood cells were also measured. There were no significant differences in TAS, H2O2 concentrations and the activity of GPx between less and more active men. In the group of more active subjects, plasma levels of GSH were higher, while TBARS concentrations were lower compared with the group of less active men (p < 0.05). Negative correlation between GSH and TBARS levels was observed (r = -0.71; p < 0.01), and GSH concentrations positively correlated with level of physical activity (r = 0.63; p < 0.05). The results indicate that habitual physical activity can favorably affect antioxidant potential and prevent lipid peroxidation in healthy, elderly men.

[Complex evaluation of body reaction in obese boys boys with systematic physical exertion and a low energy diet]. / Kompleksowa ocena reakcji organizmu otylych chlopców na systematyczny wysilek fizyczny skojarzony z dieta niskoenergetyczna.

This paper presents the results of researched influence of systematic physical effort together with a calorie restricted diet on fourteen-year-old obese boys. We evaluated their physical efficiency (VO2max), body consistency, blood lipid profile the level of non-enzymatic glycosylation of protein, insulinemia, and insulin resistance measured by the binding of 125I-Insulin to erythrocyte receptors. For 21 days, subjects performed 30 minutes of physical effort on a bicycle ergometer with load of 1 W/kg of body mass. Simultaneously, they were subjected to a calorie restricted diet at an average level of 1300 kcal. Before administering of the therapy and following its completion, the maximal amount of oxygen intake and body consistency was determined. Following that, the boy subjects performed an effort on the bicycle ergometer having an intensity of 70% VO2max and, simultaneously, capillary and venous blood was drawn for testing. In the capillary blood samples indices of acid-base balance, the lactate level, and glucose level were determined. In the venous blood samples levels of lipids, immunoreactive insulin, C-peptide and fructosamine were determined. Binding of 125I-Insulin was determined according to the method described by Gambhir and modified by us. Obtained results show that the administered therapy contributed to an increased physical efficiency, a decrease in the body's fatty mass, a reduction by 20% of insulinemia (p < 0.01) and insulin resistance as measured by the amount of 125I-Insulin binding.