ABSTRACT
Objective.To evaluate the performance of two photon-counting (PC) detectors based on different detector materials, gallium arsenide (GaAs) and cadmium telluride (CdTe), for PC micro-CT imaging of phantoms with multiple contrast materials. Another objective is to determine if combining these two detectors in the same micro-CT system can offer higher spectral performance and significant artifact reduction compared to a single detector system.Approach. We have constructed a dual-detector, micro-CT system equipped with two PCDs based on different detector materials: gallium arsenide (GaAs) and cadmium telluride (CdTe). We demonstrate the performance of these detectors for PC micro-CT imaging of phantoms with up to 5 contrast materials with K-edges spread across the x-ray spectrum ranging from iodine with a K-edge at 33.2 keV to bismuth with a K-edge at 90.5 keV. We also demonstrate the use of our system to image a mouse prepared with both iodine and bismuth contrast agents to target different biological systems.Main results.When using the same dose and scan parameters, GaAs shows increased low energy (<50 keV) spectral sensitivity and specificity compared to CdTe. However, GaAs performance at high energies suffers from spectral artifacts and has comparatively low photon counts indicating wasted radiation dose. We demonstrate that combining a GaAs-based and a CdTe-based PC detector in the same micro-CT system offers higher spectral performance and significant artifact reduction compared to a single detector system.Significance.More accurate PC micro-CT using a GaAs PCD alone or in combination with a CdTe PCD could serve for developing new contrast agents such as nanoparticles that show promise in the developing field of theranostics (therapy and diagnostics).
Subject(s)
Cadmium Compounds , Iodine , Quantum Dots , Animals , Mice , X-Ray Microtomography/methods , Contrast Media , Tellurium , BismuthABSTRACT
One of the core pathological features of Alzheimer's disease (AD) is the accumulation of amyloid plaques in the brain. Current efforts of medical imaging research aim at visualizing amyloid plaques in living patients in order to evaluate the progression of the pathology, but also to facilitate the diagnosis of AD at the prodromal stage. In this study, we evaluated the capabilities of a new experimental imaging setup to image amyloid plaques in the brain of a transgenic mouse model of Alzheimer's disease. This imaging setup relies on a grating interferometer at a synchrotron X-ray source to measure the differential phase contrast between brain tissue and amyloid plaques. It provides high-resolution images with a large field of view, making it possible to scan an entire mouse brain. Here, we showed that this setup yields sufficient contrast to detect amyloid plaques and to quantify automatically several important structural parameters, such as their size and their regional density in 3D, on the scale of a whole mouse brain. Whilst future developments are required to apply this technique in vivo, this grating-based setup already gives the possibility to perform powerful studies aiming at quantifying the amyloid pathology in mouse models of AD and might accelerate the evaluation of anti-amyloid compounds. In addition, this technique may also facilitate the development of other amyloid imaging methods such as positron emission tomography (PET) by providing convenient high-resolution 3D data of the plaque distribution for multimodal comparison.
Subject(s)
Alzheimer Disease/diagnostic imaging , Imaging, Three-Dimensional/methods , Neuroimaging/methods , Plaque, Amyloid/diagnostic imaging , Tomography, X-Ray Computed/methods , Alzheimer Disease/pathology , Animals , Brain/diagnostic imaging , Brain/pathology , Disease Models, Animal , Image Processing, Computer-Assisted , Mice , Mice, Transgenic , Plaque, Amyloid/pathologyABSTRACT
The monochromatic and polychromatic performance of a grating interferometer is theoretically analysed. The smallest detectable refraction angle is used as a metric for the efficiency in acquiring a differential phase-contrast image. Analytical formulae for the visibility and the smallest detectable refraction angle are derived for Talbot-type and Talbot-Lau-type interferometers, respectively, providing a framework for the optimization of the geometry. The polychromatic performance of a grating interferometer is investigated analytically by calculating the energy-dependent interference fringe visibility, the spectral acceptance and the polychromatic interference fringe visibility. The optimization of grating interferometry is a crucial step for the design of application-specific systems with maximum performance.