[characteristics of the bone tissue metabolism in adolescents with the connective tissue's undifferentiated dysplasia].

Examination of the markers of bone turnover (osteocalcin, N-terminal propeptide of type I collagen PINP and beta-isomerized carboxy-terminal cross-linking region of collagen type I beta-CrossLaps) was carried out in 17 adolescents at the age of 11-14 years with the connective tissue's undifferentiated dysplasia. There was the decreased content of the bone-formation markers (osteocalcin and PINP) in the blood serum compared to the control group (16 healthy adolescents at the corresponding age and gender) in the normal rate of the bone destruction marker (beta-CrossLaps), it is indicative of the process abnormality of the bone remodeling characterized by the intensity loss of the bone tissue formation with relative absorption predominance. The revealed disorders of the bone metabolism contribute to the osteopenia progression. It allows to attribute the adolescents with the connective tissue's undifferentiated dysplasia to the risk group in terms of the early osteoporosis.

[The characteristics of regulation of bone remodeling under inherent pathology of locomotive system in children].

The article presents the results of evaluation of clinical pathogenic value of hormones, markers of metabolism and indicators of mineral metabolism information of inherent pathology of locomotive system in children. The sampling included 29 children with dysplasia and deformation of lower extremities and 35 children without pathology of locomotive system. All children aged from 6 to 12 years. The serum levels of parathormone, calcitonin and 25(OH)-D3 were established using analytic module platform "Cobas 6000 SWA" (Roche Diagnostics, Switzerland). The content of somatotropic hormone in blood serum was evaluated using analyzer "Immulite One" (USA). The single examination of serum concentrations of total and ionized calcium, phosphorus, magnesium and activity of alkaline phosphatase was implemented using automatic analyzer "Cobas 6000 SWA" (Roche Diagnostics, Switzerland) and "HITACHI-912" (Roche Diagnostics corporation, Indianapolis, IN, USA). The activity of process of formation of and resorption of bone tissue was evaluated according content of PINP (N-terminal propeptid of type I collagen), osteocalcin and beta-CrossLaps (beta-isomerized carboxy-terminal cross-linking region of collagen type I) in blood serum. The module platform "Cobas 6000 SWA" (Roche Diagnostics, Switzerland) was used. The analysis of correlation interrelationships between qualitative indicators of bone metabolism and levels of regulative hormones in children with inherent pathology of locomotive system made it possible to clarify possible aspects of pathogenesis of disorders of bone remodeling as a result of induction of synthesis of somatotropic hormone and parathormone. The complex multi directional impact of these hormones results in uncoupling of synthesis processes and bone tissue resorption against the background of total slowing-down of bone remodeling. These occurrences apparently promote formation of dysplasia and deformation of bone skeleton.

[The diagnostic of anaerobic infection induced by Clostridium perfringens in patient with post-traumatic phlegmon: a clinical case].

The article presents uncommon clinical case of anaerobic gas-producing infection induced by Clostridium perfringens. The disease resulted in lethal outcome at fourth day after admission of patient into hospital. The successful treatment requires timely diagnostic of clostridium infection based on complex of microbiologic, clinical and laboratory data. The early diagnostic is possible in case of bacteriologic analysis of native material.

[Microbiol landscape and production of beta-lactamases with extended spectrum activity in patients with urinary tract infection].

Urinary tract infections (UTIs) are widespread in Russia. One of the major problems facing practical medicine in the country is laboratory diagnosis of pyoinflammatory diseases of the urogenital tract caused by opportunistic microorganisms. A major difficulty in this context is posed by polyresistance of certain Gram-negative bacteria (E.coli, Klebsiella spp., etc.) associated with their production of a great variety of beta-lactamases (BL). This study was designed to determine species composition of urogenital tract pathogens and evaluate their resistance to antibacterial preparations with special reference to the mechanism of resistance of BL production to this drugs. The study included 169 patients with urinary tract infections admitted to the Clinical Research Centre for Healthcare of Miners, Leninsk-Kuznetsk, between 01.02.2007 and 0.1.02.2008

[The effect of extracorporeal thermal-modified autologous plasma exchanges on dynamics of hormone-metabolic homeostasis indices in patients with acute myocardial infarction]. / Vliianie kursa plazmoobmenov ékstrakorporal'no termicheski modifitsirovannoi autoplasmy na dinamiku pokazatelei gormonal'no-metabolicheskogo gomeostaza u bol'nykh ostrym infarktom miokarda.

Changes of biochemical indexes in the blood of 56 patients with acute myocardial infarction against the background of conventional and complex treatment using plasma exchange of extracorporeal-termally modified autoplasma have been analysed. The findings show that complex treatment of patients with AMI, using plasma exchange of extracorporal-thermally modified autoplasma, leads to much earlier decrease of KPK, LDH, LDH-1 enzyme activity in blood; it indicates the reduction of the period of myocardiocyte function restoration. The usage of plasma exchange of extracorporeal-thermally modified autoplasma in patients with acute myocardial infarction is accompanied by the absence of increase of glucose concentration in blood (owing to the normalization of insulin production), favourable influence on stress-reaction of biological systems of organism decrease of atherogenity index. Optimisation and efficiency of AMI therapy during treatment in the hospital is possible, with plasma exchange of extracorporeal-thermally modified autoplasma included in complex therapy.

[The assessment of liver function in local suppurative processes (experimental research)]. / Otsenka funktsional'nogo sostoianiia pecheni pri lokal'nykh gnoinykh protsessakh (éksperimental'noe issledovanie).

The function of the liver was studied on an experimental model of a local purulent process in 40 rabbits. It is shown that a local purulent process may cause a hepatic damaging effect, the presence of which is confirmed by the active egress of the organ-specific enzymes histidase and urocaninase into the blood. Determination of these enzymes in blood serum is an authentic test which characterizes damage to the hepatic cells in a local purulent process. Appraisal of the functional condition of the liver in local purulent processes may be employed in clinical practice for early detection and timely treatment of possible hepatotoxic damage.

[Effect of subcutaneous administration of liposomes on the functional state of the organs and systems in experimental animals]. / Vliianie podkozhnogo vvedeniia liposom na funktsional'noe sostoianie organov i sistem éksperimental'nykh zhivotnykh.

Based on the study of subcutaneous injection of lecithin-cholesterol liposomes, its possible toxic effect is discussed and the effect threshold is determined. It is shown, that long-term subcutaneous liposome administration is attended with hepatic cell damage. One or two injections of the preparation does not cause a toxic lesion of the viscera and may be used for therapeutic purposes. A considerable threshold-subthreshold dosage range allows for vast variability of administered liposome amounts.