[Formation of nitrosyl complexes of non-heme iron in the blood of animals in vivo]. / Obrazovanie nitrozil'nykh kompleksov negemovogo zheleza v krovi zhivotnykh in vivo.

Addition of an increased content of iron together with nitrit to the diet results in the formation of nitrozyl complexes of non-hem iron in rats' blood. The same complexes are recorded 9 hours after intraperitoneal injection of water solution of iron nitrozyl complex with cistein. Binding of non-hem iron nitrozyl complexes with RS-groups of blood protein is discussed. Nitrozyl complexes of non-hem iron in regular blood elements in vitro are obtained.

[Formation of nitrosyl complexes of nonheme iron (2.03 complexes) in animal tissues in vivo]. / Obrazovanie nitrozil'nykh kompleksov negemovogo zheleza (kompleksov 2.03) v tkaniakh zhivotnykh in vivo.

Formation or dinitrozyl non-haem iron complexes (2.03 complexes) in white rats' tissues in vivo has been studied. The formation of these complexes has been examined in the liver kidneys and small intestines when injecting p/o NaNO2 to the rats. Most of the complexes have been found in the liver. They have not been detected in the heart, spleen, muscle, cerebrum and marrow in vivo. 3-4-fold content of 2.03 complexes has been determined while introducing iron and NaNO2 in running water. This effect has not been found when introducing iron and NaNO2 simultaneously. The mechanism of 2.03 complex formation in the animal tissues in vivo has been proposed. According to this mechanism these complexes are formed in the tissues when Fe-NO from nitrozyl non-haem from complexes originating in blood passes into SH-group of protein in tissues. The formation of 2.03 complexes in vivo has been found in blood uniform elements.

[Mechanism of the formation of nitrosyl complexes of non-heme iron in animal tissues in vivo]. / O mekhanizme obrazovaniia nitrozil'nykh kompleksov negemovogo zheleza v tkaniakh zhivotnykh in vivo.

Further investigations were carried out into the formation mechanism of nitrosyl complexes of non-heme iron (complexes 2.03) in rat liver and blood at intrauterine injection of aqueous solutions of nitrosyl complexes of iron with cysteine, thiosulphate, phosphate, ATP and hydroxyl, as well as at keeping the animals on nitrite and iron diet. It has been found that 5-6 hours after the injection of iron nitrosyl complexes with cysteine/the shape of ESR signal with gav = 2.03 in the liver becomes identical to the shape of the signal obtained after adding nitrite and iron to the animal food. Therefore it is assumed that in spite of different methods of induction, the 2.03 complexes formed in these experiments are identical in structure and localization. The assumption was proved as to initial formation of Fe-NO in the blood with subsequent transition to SH-groups of liver proteins. It is suggested that complexes 2.03 in vivo are concerned with a specific type of protein.

[Discovery of endogenous nitric oxide in the mouse liver by electron paramagnetic resonance]. / Obnaruzhenie éndogennoi okisi azota v pecheni myshei metodom élektronnogo paramagnitnogo rezonansa.

In vivo formation of nitrogen oxide in the mouse liver kept on purely protein diet (casein) was shown by ESR method. This phenomenon is suggested to be conditioned by the oxidation of ammonia accumulated in the organism as a result of desamination of casein amino acids.

[The antioxidant treatment of patients with acute intestinal infections due to gram-negative microorganisms]. / Lechenie antioksidantami bol'nykh ostrymi kishechnymi infektsiiami, vyzvannymi gramotritsatel'nymi mikroorganizami.

It was established that administration of S. typhimurium lysate causes activation of the processes of free-radical oxidation. Introduction of unithiol in combination with magnesium sulfate returns the intensity of these processes to the initial level. Unithiol and alpha-tocopherol acetate protect the animals form lethal effects of S. sonnei lysate. Use of a combination of pharmacological agents consisting of unithiol, magnesium sulfate and alpha-tocopherol acetate in the treatment of patients with acute intestinal infections due to gram-negative pathogens (shigellae, salmonellae, proteus, klebsiellae, pseudomonas) resulted in decrease of the length of the intoxication and diarrhea syndrome, decrease of the frequency of repeat bacteria elimination and protracted forms of these infections, more rapid normalization of immune system functions.