ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Mangaba (Hancornia speciosa Gomez) is a medicinal plant frequently cited in ethnopharmacological inventories of the central region of Brazil against gastrointestinal disorders such as diarrhoea, ulcer, gastritis and stomach ache. AIM OF THE STUDY: The hydroalcoholic extract (HE) and infusion (BI) of Hancornia speciosa bark were investigated for their ability to prevent and heal rodent gastric ulcer. MATERIALS AND METHODS: The preventive and healing action of both preparations of Hancornia speciosa were evaluated in experimental models in rodents that simulated this disease in human gastric mucosa. RESULTS: BI did not exert gastroprotective effect, in contrast to HE (500mg/kg, p.o.) that decreased (p<0.05) the severity of gastric damage induced by HCl/ethanol (52%), indomethacin/bethanechol (51%), stress (52%) or pylorus ligature experiments (54%). HE increased (p<0.05) the pH and decreased acid output of gastric juice. This extract promoted increase of mucus amount (3.62mg/wt. tissue vs. 5.81mg/wt. tissue), healing action (67%) and displayed anti-Helicobacter pylori effect. CONCLUSIONS: The antiulcer action of Hancornia speciosa resulted in increase of gastric mucus formation and antioxidant properties of polymeric proanthocyanidins present in the bark composition of this medicinal plant.
Subject(s)
Anti-Ulcer Agents/pharmacology , Apocynaceae/chemistry , Plant Extracts/pharmacology , Stomach Ulcer/drug therapy , Animals , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Anti-Ulcer Agents/administration & dosage , Anti-Ulcer Agents/isolation & purification , Antioxidants/administration & dosage , Antioxidants/isolation & purification , Antioxidants/pharmacology , Brazil , Disease Models, Animal , Gastric Juice/drug effects , Gastric Juice/metabolism , Gastric Mucosa/drug effects , Gastric Mucosa/metabolism , Gastric Mucosa/pathology , Helicobacter pylori/drug effects , Humans , Hydrogen-Ion Concentration/drug effects , Male , Medicine, Traditional , Mice , Plant Bark , Plant Extracts/administration & dosage , Proanthocyanidins/administration & dosage , Proanthocyanidins/isolation & purification , Proanthocyanidins/pharmacology , Rats , Rats, WistarABSTRACT
AIM OF THE STUDY: To assess the anti-inflammatory effect of butanolic fraction of methanolic extract from bark of Abarema cochliacarpos in acute ulcerative colitis model induced by intracolonic administration of trinitrobenzene sulfonic acid (TNBS) in Wistar rats. MATERIALS AND METHODS: Abarema cochliacarpos (100 and 150mg/kg/day) was administered by gavage 48, 24 and 1h prior to the induction of colitis with 10mg/kg of TNBS and, 24h later. RESULTS: Phytochemical studies by mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) revealed that catechins were a major component into condensate class of tannins. Treatment with Abarema cochliacarpos decreased significantly macroscopic damage as compared with TNBS (p<0.05). Histological analysis showed that both doses of the extract improved the microscopic structure and preserved some areas of the colonic mucosa structure. In addition, myeloperoxidase activity (MPO), as a marker of neutrophil infiltration, was decreased in a dose-dependent way (p<0.01 and p<0.001 respectively), TNF-alpha level was also diminished with the highest dose of the extract (p<0.001) and, IL-10 level obtained no significant results. In order to elucidate some of the mechanisms, expression of inducible inflammatory enzymes, such as cyclooxygenase (COX)-2 and nitric oxide synthase (iNOS), were studied showing a significant reduction. Finally, the involvement of c-Jun N-terminal kinase (JNK) signalling demonstrated a reduction in the JNK activation with the highest dose (p<0.05 vs TNBS). CONCLUSIONS: We have shown for the first time that the extracts obtained from Abarema cochliacarpos bark possess active substances, which exert marked protective effects in acute experimental colitis, confirming and justifying, at least in part, the popular use of this plant to treat gastrointestinal diseases.