Design, synthesis and anticancer activity of 5-((2-(4-bromo/chloro benzoyl) benzofuran-5-yl) methyl)-2-((1-(substituted)-1H-1,2,3-triazol-4-yl)methoxy)benzaldehyde analogues.

Novel 5-((2-(4-bromo/chloro benzoyl) benzofuran-5-yl) methyl)-2-((1-(substituted)-1H-1,2,3-triazol-4-yl)methoxy)benzaldehyde analogues about twenty-one were synthesized all through standard chemical procedures. The structure of the compounds were confirmed by 1H NMR, 13C NMR and Mass spectral analysis after purification. All the compounds were screened for In Vitro lung and cervical cancer activity against A-549 and HeLa cell lines, respectively, by MTT assay protocol using various nanomolar (nM) concentrations. IC50 value were calculated from cell viability data. 2-(trifluoromethyl)benzyl substituted derivative presented outstanding activity against both the cell lines compared to standard drug doxorubicin. The methoxy, chloro, fluoro and formyl substituted analogues showed a moderate activity and whereas methyl substituted analogues activity was poor. The morphological deformation of both cell lines by best IC50 value analogues proved as potent inhibitors of cancer cells growth. Molecular docking studies were performed against extracellular signal-regulated kinase 2 and fibroblast growth factor receptor 2 these results are incredibly in agreement with the investigational data.

Narrative review on artificially intelligent olfaction in halitosis.

Halitosis, commonly known as oral malodor, is a multifactorial health concern that significantly impacts the psychological and social well-being of individuals. It is the third most frequent reason for individuals to seek dental treatment, after dental caries and periodontal diseases. For an in-depth exploration of the topic of halitosis, an extensive literature review was conducted. The review focused on articles published in peer-reviewed journals and only those written in the English language were considered. The search for relevant literature began by employing subject headings such as 'halitosis, oral malodor, volatile sulfur compounds, artificial intelligence, and olfaction' in databases such as PubMed/Medline, Scopus, Google Scholar, Web of Science, and EMBASE. Additionally, a thorough hand search of references was conducted to ensure the comprehensiveness of the review. After amalgamating the search outcomes, a comprehensive analysis revealed the existence of precisely 134 full-text articles that bore relevance to the study. Abstracts and editorial letters were excluded from this study, and almost 50% of the full-text articles were deemed immaterial to dental practice. Out of the remaining articles, precisely 54 full-text articles were employed in this review. As primary healthcare providers, dentists are responsible for diagnosing and treating oral issues that may contribute to the development of halitosis. To effectively manage this condition, dentists must educate their patients about the underlying causes of halitosis, as well as proper oral hygiene practices such as tongue cleaning, flossing, and selecting appropriate mouthwash and toothpaste. This narrative review summarises all possible AI olfaction in halitosis.

Applications of robot-assisted UV disinfection in dentistry.

Maintaining a microbial-free environment in healthcare facilities is more widely recognized as an essential component of therapies to minimize transmission of viruses associated with healthcare sector. The global spread of COVID-19 and recent outbreaks have presented humanity with previously unheard-of challenges. The development of autonomous disinfection robots seems to be necessary given the urgent need for constant sterilization in the face of a labor shortage. Due to their automated and perceived cost advantages by eliminating cleaning staff, these robots are being advertised more and more as an easy solution to immediately disinfect rooms and operating areas. The use of these services lowers the danger of infection, and expense of traditional cleaning and, most significantly, builds trust and security in medical facilities. Currently, routine (manual) cleaning is not replaced by disinfection robots; rather, they may support it. Additional hospital and device design alterations are necessary to address the overshadowing (visibility) issue allowing Ultraviolet disinfectant (UV-D) robots move freely in the medical environment. More technical developments and clinical studies in a variety of hospitals are needed to overcome the current challenges and find ways to integrate this unique technology into hospitals now and in the future. Thus, we present a review that includes detailing all elements required for it to function, as well as both its advantages and disadvantages. To the best of our knowledge, very limited studies have collected an in- depth data on the sterilization effect using a disinfection robot in the field of dentistry. We believe that this data will work as a foundation in more advanced uses at diverse sites that require disinfection and will highlight unsolved challenges and potential research avenues for UV robot operational concerns in dental hospitals. The goal of this work is to offer a comprehensive manual for UV-D robots covering pertinent information on traditional Ultraviolet germicidal irradiation (UVGI) system along with advancements in UV-D robots and thereby focusing on in-depth application in medical and dental facilities.

Are WHO approved nucleic acid amplification tests causing large-scale "false identification" of rifampicin-resistant tuberculosis?: Programmatic experience from south india.

INTRODUCTION: The nucleic acid amplification tests (NAATs): Line probe assay and GeneXpert (Xpert) have evolved as the primary tool for identification of rifampicin (RIF)-resistant (RR) tuberculosis (TB) worldwide, primarily because of the ease and speed. We rechecked RR isolates identified by NAATs from presumptive RR TB cases belonging to South India by the Revised National TB Control Program, India using multiple RIF concentrations on Bactec MGIT system and compared the mutation patterns with the resistance levels. METHODOLOGY: Standard protocol for Bactec MGIT system as given by the manufacturer modified for the multiple RIF concentrations was used. All the retests were done in a certified BSL3 laboratory. RESULTS: We found that there is a mismatch of up to 20% (RIF breakpoint 0.5 mg/L); the NAATs probably overidentifying RR TB. Half of the cases with mismatch showed a sub-breakpoint rise in resistance level (0.125 mg/L to 0.5 mg/L RIF). DISCUSSION AND CONCLUSION: The probable reasons for the mismatch are "sub-breakpoint low-level resistance mutants," hetero-resistant bacterial populations, and other inherent test limitations along with the low RR TB prevalence in South India (<5%) among "presumptive multidrug-resistants." This could be due to the incomplete selection pressure by an inadequate RIF exposure caused by various factors including a low-RIF dosage being used widely and poor Directly observed treatment. To prevent the false diagnosis of RR TB in a massive scale when using NAATs, we may need to enforce a carefully targeted testing approach and a phenotypic susceptibility testing with multiple RIF concentrations for confirmatory purposes.