The effects of hyperbaric oxygen therapy on neuroprotection and recovery after brain resuscitation.

OBJECTIVE: Analyze the impact of hyperbaric oxygen therapy on neuroprotection and recovery post severe traumatic brain injury (sTBI) resuscitation. METHODS: Retrospective analysis of clinical data from 83 sTBI patients admitted between January 2022 to January 2024. Patients were divided into control (n = 41) and observation (n = 42) groups based on treatment received. Control received standard therapy, while the observation group received hyperbaric oxygen therapy. Effects on clinical outcomes, neuroinjury markers (S100ß, GFAP, UCH-L1, NSE), neurotrophic factors (NGF, BDNF), neurological function indicators (NIHSS, CSS), and adverse reactions were compared. RESULTS: The observation group showed a higher total effective rate (80.95%) compared to control (60.98%) (p < 0.05). Neuroinjury markers decreased post-treatment in both groups, with the observation group lower (p < 0.05). NGF and BDNF levels increased post-treatment in both groups, with the observation group higher (p < 0.05). NIHSS and CSS scores decreased post-treatment in both groups, with the observation group lower (p < 0.05). No significant difference in adverse reactions between groups (p > 0.05). CONCLUSION: Hyperbaric oxygen therapy effectively treats sTBI by improving brain resuscitation success, reducing neuroinjury factors, enhancing neurotrophic factors, and promoting neurological function recovery, without increasing adverse reaction risk.

Revealing the link between evolution of electron transfer capacity of humic acid and key enzyme activities during anaerobic digestion.

Humic acid (HA) is an important active compound formed during anaerobic digestion process, with a complex structure and dynamic electron transfer capacity (ETC). However, the mechanisms by which these macromolecular organic compounds dynamically interact with the microbial anaerobic digestion process at different operating temperatures are still unclear. In this study, the link between the evolution of the ETC of HAs and the microbial community under mesophilic and thermophilic conditions was investigated. The results showed an increasing trend in the ETC of HAs in both mesophilic (671-1479 µmol gHA-1) and thermophilic (774-1506 µmol gHA-1) anaerobic digestion (AD) until day 25. The ETC was positively correlated with the bacterial community of hydrolytic and acidogenic phases, but negatively correlated with the archaeal community of the methanogenic phase. Furthermore, the relationship between ETC and key enzyme activity was explored using a co-occurrence network analysis. HAs revealed a high potential to promote key enzyme activities during hydrolysis (amylase and protease) and acidification (acetate kinase, butyrate kinase, and phosphotransacetylase) while inhibiting the key enzyme activity in the methanogenic phase during the anaerobic digestion process. Moreover, HAs formed under thermophilic conditions had a greater influence on key enzyme activities than those formed under mesophilic conditions. This study advances our understanding of the mechanisms underlying the influence of HAs on anaerobic digestion performance.

Simulation, prediction and optimization of typical heavy metals immobilization in swine manure composting by using machine learning models and genetic algorithm.

Machine learning (ML) is a novel method of data analysis with potential to overcome limitations of traditional composting experiments. In this study, four ML models (multi-layer perceptron regression, support vector regression, decision tree regression, and gradient boosting regression) were integrated with genetic algorithm to predict and optimize heavy metal immobilization during composting. Gradient boosting regression performed best among the four models for predicting both heavy metal bioavailability variations and immobilization. Gradient boosting regression-based feature importance analysis revealed that the heavy metal initial bioavailability factor, total phosphorus, and composting duration were the determinant factors for heavy metal bioavailability variations (together contributing >75%). After genetic algorithm optimization, the maximum immobilization rates of Cu, Zn, Cd, As, and Cr were 79.53, 31.30, 14.91, 46.25, and 66.27%, respectively, superior to over 90% of the measured data. These findings demonstrate the potential application of ML to risk-control for heavy metals in livestock manure composting.

Analysis of reactive phosphorus treatment by filter materials at the edge of tile-drained agricultural catchments: A global view of the current status and challenges.

Phosphorus losses from agriculture have long generated concern due to the ecological impact on surface waters. Here tile-drained agricultural catchments are a critical source for concentrating and transporting phosphorus bioavailable forms or dissolved reactive phosphorus (DRP). Hence, edge-of-field technologies have been introduced to reduce DRP loads. Filter systems have received special attention due to their targeted approach using a permeable filter material (FM) rich in DRP sorbents. This review explores the performance and applicability of FMs in the aforementioned context because of the growing number of studies. An overall analysis revealed that sorption is preferable to precipitation for DRP retention at the edge-of-field, and that FM pH and particle size affect sorption properties and subsequently DRP retention and lifetime. Thus, FMs with predominant amounts of iron and/or aluminium can be recommended. Such materials generally have an appreciable availability of DRP binding sites, strong bonds with DRP and short reaction times, as well as low desorption, which lead to good operation. On the other hand, FMs with predominant amounts of calcium and/or magnesium are restricted to catchments with favourable conditions unless they have optimal reactivity for DRP. The review also found that hydraulic retention time plays a key role in the performance and applicability of FMs, especially in those dependent on precipitation reactions. Therefore, it is crucial that FMs are designed, constructed and managed according to the catchment conditions-including normally varying flow rates and DRP concentrations-in order to ensure successful operation. This reflects in long-term, high and steady net DRP retention along with low costs, thus improving the FM cost-effectiveness, besides discharging non-harmful effluents to aquatic ecosystems.

Probing changes in humus chemical characteristics in response to biochar addition and varying bulking agents during composting: A holistic multi-evidence-based approach.

Despite the various benefits of humus, the changes in its chemical characteristics during composting in response to biochar addition and varying bulking agents remain to be further explored. In this study, three treatments were conducted, in which swine manure, bulking agent, and biochar were mixed at ratios of 4:1:0, 8:1:0, and 8:1:1. Fourier transform infrared spectroscopy (FTIR), carbon nuclear magnetic resonance spectroscopy (13C-NMR), three-dimensional excitation-emission matrix fluorescence spectroscopy (3D-EEM), and near-edge X-ray absorption fine structure (NEXAFS) were employed to characterize the chemical and structural properties of humus from multiple perspectives. The 3D-EEM spectra in this study showed a larger increase in humic acids (HAs) content (56%) and HAs to fulvic acids ratio (128%) during composting, indicating stronger humification in biochar-amended treatment. FTIR, 13C-NMR, and NEXAFS all confirmed the essential properties of HA as the core agronomic functional substance with rich aromatic and carboxyl groups, and that its aromaticity increased gradually during composting. In addition, 13C-NMR demonstrated that biochar addition and a relatively higher bulking agent ratio aided an increase in the carboxyl C proportion in HA after composting. In particular, NEXAFS revealed that biochar addition promoted the diversification of C, N, and O species in HA, with the emergence of quinone C and O-alkyl C as the main representatives. This work suggests that biochar addition and a relatively high bulking agent ratio could enhance humification and improve the agronomic function of humus.

Long-term performance of three mesophilic anaerobic digesters to convert animal and agro-industrial wastes into organic fertilizer.

Few studies have investigated the performance of anaerobic digestion (AD) to convert animal and agro-industrial wastes to organic fertilizers over a long-term field conditions. This paper studied three large-scale mesophilic digesters (D1eD3) over two years for their effects on feedstocks, which were dairy manure for D1 and D2 and co-digestion mixed manure and agro-industrial wastes for D3. Hydraulic retention times (HRT) were 9 d for D1, 12 d for D2, and 34 d for D3. Digester influent and effluent samples were taken every two months from the digesters and analyzed for pH, and concentrations of total solids (TS), ammonium nitrogen (NH4-N), total Kjeldahl nitrogen (TKN), total phosphorus (TP), and eight metals. The study revealed high variability in converting feedstock in the three digesters. Compared with their respective influent, the mean digester effluent pH decreased from 7.9 by 0.6 in D1 (p < 0.01) and by 0.3 in D2 (p < 0.01), but it increased from 6.1 by 1.8 in D3 (p < 0.01). The mean digester effluent TS increased from 3.4% by 0.1% (p > 0.05) in D1, but it decreased from 4.9% by 1.3% in D2 (p < 0.05) and from 12.3% by 4.8% in D3 (p < 0.01). All three digesters significantly increased NH4-N concentrations by 21.4 e81.8% (p < 0.05), but insignificantly changed TKN and TP concentrations (p > 0.05). Effects of AD on all metal concentrations were mixed and were insignificant (p > 0.05) because of large concentration variations. However, study of a ratio quotient (q Mg ) using magnesium (Mg) as the reference discovered accumulation of NH4-N, copper, potassium, and sodium, but loss of TKN, TP, iron, manganese, zinc, and calcium during AD for D2 and D3. The impact of AD conversion was closely related with types of feedstock (on pH) and HRT (on TS and NH4-N). The results of this study can assist in developing strategies for cleaner production using AD in an environmentally sustainable manner.

Probing the efficiency of magnetically modified biomass-derived biochar for effective phosphate removal.

Characterization of the driving forces for effective and economical phosphate (PO43-) removal from wastewater by using magnetically modified biochar was performed in this study. The biochar produced from slow pyrolysis of local agricultural biomass (wood and rice husks) were magnetically modified by co-precipitation of Fe(II) and Fe(III) ions in their presence. The surface characteristics before and after modification and their efficacy for PO43- sorption, and desorption were compared. Results show that, even though magnetic biochar surface modification slightly decreased their surface area, PO43- adsorption to the modified biochars was almost double (25-28 mg g-1) than that to the raw biochar (12-15 mg g-1). The adsorption isotherm of raw biochars was better simulated via the Langmuir model, while that of modified biochars was better fitted to the Freundlich model. Moreover, the integrated analysis by XRD, EDX, and FTIR show that PO43- sorption to modified biochars could be attributed to the simultaneously-occurring electrostatic attraction, surface precipitation, and ligand exchange. While the electrostatic attraction was dominant in the presence of unmodified biochars. The regenerated modified biochars retained substantial PO43- adsorption capacity up to several regeneration cycles. Their high reusability potential leads to the effective and economical phosphate recovery and thus modified biochars could offer a viable strategy for PO43- removal.

Microbial community responses to agricultural biomass addition in aerated constructed wetlands treating low carbon wastewater.

Using agricultural biomasses as solid carbon substrates in constructed wetlands (CWs) could be an effective way to achieve sustainable nitrogen removal for carbon-limited wastewater treatments. This study investigated the response of bacteria community in CWs to the addition of agricultural biomasses (wheat straw, walnut shell and apricot pit). Results indicated that the addition of different agricultural biomasses had distinct influence on bacterial communities in CWs. Both wheat straw and walnut shell increased the diversity of microbial communities and optimized the structure of microorganisms. The effect of apricot pit on the richness and evenness of microbial communities was not significant, but the composition of microorganisms was significantly affected at the phylum level, especially the relative abundance of phylum Saccharibacteria. Moreover, the addition of agricultural biomasses in CWs acclimatized more functional bacteria including nitrifier and denitrifier, which were proved to be positively correlated with the high-rate denitrification performance. The obtained results would be beneficial to understand the underlying microbial mechanism of nitrogen removal in CWs with agricultural biomass and provide some guidance on the practical application of CWs.

RNA-sequencing analysis of shell gland shows differences in gene expression profile at two time-points of eggshell formation in laying chickens.

BACKGROUND: Eggshell formation takes place in the shell gland of the oviduct of laying hens. The eggshell is rich in calcium and various glycoproteins synthesised in the shell gland. Although studies have identified genes involved in eggshell formation, little is known about the regulation of genes in the shell gland particularly in a temporal manner. The current study investigated the global gene expression profile of the shell gland of laying hens at different time-points of eggshell formation using RNA-Sequencing (RNA-Seq) analysis. RESULTS: Gene expression profiles of the shell gland tissue at 5 and 15 h time-points were clearly distinct from each other. Out of the 14,334 genes assessed for differential expression in the shell gland tissue, 278 genes were significantly down-regulated (log2 fold change > 1.5; FDR < 0.05) and 413 genes were significantly up-regulated at 15 h relative to the 5 h time-point of eggshell formation. The down-regulated genes annotated to Gene Ontology (GO) terms showed anion transport, synaptic vesicle localisation, organic anion transport, secretion and signal release as the five most enriched terms. The up-regulated gene annotation showed regulation of phospholipase activities, alanine transport, transmembrane receptor protein tyrosine kinase signalling pathway, regulation of blood vessels diameter and 3, 5-cyclic nucleotide phosphodiesterase activity as the five most enriched GO terms. The putative functions of genes identified ranged from calcium binding to receptor activity. Validation of RNA-Seq results through qPCR showed a positive correlation. CONCLUSIONS: The down-regulated genes at 15 h relative to the 5 h time-point were most likely involved in the transport of molecules and synthesis activities, initiating the formation of the eggshell. The up-regulated genes were most likely involved in calcium transportation, as well as synthesis and secretory activities of ions and molecules, reflecting the peak stage of eggshell formation. The findings in the current study improve our understanding of eggshell formation at the molecular level and provide a foundation for further studies of mRNA and possibly microRNA regulation involved in eggshell formation in the shell gland of laying hens.

Critical Review: Biogeochemical Networking of Iron in Constructed Wetlands for Wastewater Treatment.

Iron is present in all types of wastewater; however, besides acid mine drainage, where it is a major constituent of concern, it is usually neglected in other types of wastewaters. In all kinds of constructed wetlands, iron plays important role in removal of organics and phosphorus, and it has an impact on transformation of nitrogen, sulfur, and metals. The biogeochemistry of iron is well understood in natural wetlands, but knowledge about iron impact on microbiological and chemical transformations during wastewater treatment in constructed wetlands is very limited. So far, the sparse research in this area provides limited information on observed interactions with several varying parameters across the studies, making it difficult to draw fundamental and mechanistic conclusions. A critical review of the complex biogeochemical networking of iron in CWs is therefore necessary to fill the gap in knowledge on the role of iron and its biogeochemical multi-interactions in wastewater treatment processes of CWs. This review is the first with specific focus on iron, discussing its mitigation and retention in CWs with different configurations and operational strategies, and presenting both seasonal dynamics and the potential remobilization of Fe. It also comprehensively discusses the interactions of redox-controlled iron turnover with the biogeochemical processes of other elements, for example, carbon (C), nitrogen (N), phosphorus (P), sulfur (S), and heavy metals. The health response of wetland plants to both deficiency and toxicity of Fe in CWs designed with specific treatment targets has also been evaluated. Due to the complexity of various wastewater compositions and microredox gradients in the root rhizosphere in CWs, future research needs have also been identified.

Exploring Utilization of Recycled Agricultural Biomass in Constructed Wetlands: Characterization of the Driving Force for High-Rate Nitrogen Removal.

Improper treatment of various wastewaters with a low C/N ratio and management of abundant agricultural wastes may pose a serious threat to bodies of water and agricultural ecosystems in rural areas, especially in developing countries. Thus, a potential alternative for simultaneous mitigation of this pollution is needed to protect rural environments. This study investigated the feasibility and enhanced performance of applying typical agricultural wastes (such as wheat straw, apricot pits, and walnut shells) as carbon sources for nitrogen removal in constructed wetlands (CWs). The leaching experiment employed fluorescence excitation-emission spectrophotometry and revealed that the wheat straw material had the highest capability of carbon release with an average dissolved organic carbon release content and rate of 27.88 mg g-1 and 5.24 mg g-1 day-1, respectively. Dissolved organic matter released from different agricultural wastes mainly consisted of humic acid-like and fulvic acid-like compounds. Long-term assessment of lab-scale intermittent aeration CWs receiving agricultural wastes revealed a high total nitrogen removal of 66.75-93.67% in low carbon/nitrogen ratio wastewaters (C/N = 3). These findings can contribute to a better understanding of the driving mechanism through which agricultural wastes enhance nitrogen removal in CW wastewater treatments.

Formation of struvite from agricultural wastewaters and its reuse on farmlands: Status and hindrances to closing the nutrient loop.

To meet the needs of a fast growing global population, agriculture and livestock production have been intensified, resulting in environmental pollution, climate change, and soil health declining. Closing the nutrient circular loop is one of the most important sustainability factors that affect these issues. Apart from being a serious environmental issue, the discharge of N and P via agricultural wastewater is also a major factor that disturbs nutrient cycling in agriculture. In this study, the performance, in terms of recovery, of N and P (individually, as well as simultaneously) from agricultural wastewaters via struvite has been comparatively summarized. Details on the hindrances to nutrient recovery through struvite formation from agricultural effluents, along with strategies to overcome these hindrances, are provided. In addition, various strategies for recovery performance intensification and operational cost reduction are comprehensively discussed. This work will provide scientists and engineers with a better idea on how to solve the bottlenecks of this technique and integrate it successfully into their treatment systems, which will ultimately help close the nutrient loop in agriculture.

Upregulation of genes encoding digestive enzymes and nutrient transporters in the digestive system of broiler chickens by dietary supplementation of fiber and inclusion of coarse particle size corn.

BACKGROUND: Measures to improve bird performance have been sought due to the imminent phase out of in-feed antibiotics in poultry and continued demand for higher poultry feeding efficiency. Increasing grain particle size and dietary fibre may improve gizzard function, digestive efficiency and nutrient absorption. This study was conducted to evaluate the effect increased particle size of corn and inclusion of sugarcane bagasse (SB) on mRNA expression of genes encoding digestive enzymes and nutrient transporters in broilers. RESULTS: A total of 336 day-old Ross 308 males were assigned in a 2 × 2 factorial arrangement of treatments with corn particle size - coarse 3576 µm or fine 1113 µm geometric mean diameter, and SB - 0 or 2% inclusion. Feed conversion ratio (FCR), weight gain and feed intake were measured from d 0-10 and d 10-24. The relative gizzard weight and mRNA expression of genes encoding digestive enzymes and intestinal nutrient transporters were measured on d 24. During d 10-24, a particle size × SB interaction was observed for FCR (P < 0.01), where birds fed coarsely ground corn (CC) with 2% SB had lower FCR than those fed CC without SB. A particle size × SB interaction was observed for both expression of pepsinogen A and C (P < 0.01) which were negatively correlated with FCR on d 24. Addition of 2% SB upregulated pepsinogen A and C only in CC fed birds. Further, 2% SB also upregulated pancreatic amylase (AMY2A) and intestinal cationic amino acid transporter-1 (CAT1). Inclusion of dietary CC upregulated duodenal amino peptidase N (APN), jejunal alanine, serine, cysteine and threonine transporter-1 (ASCT1), and ileal peptide transporter-2 (PepT2). CONCLUSION: These results suggest that both SB and coarse particle size modulate expression of genes encoding important digestive enzymes and nutrient transporters and thus are directly related to bird performance. These findings provide insights into the combination effects of dietary fiber and particle size in the future management of broiler feeding.

Immune responses following experimental infection with Ascaridia galli and necrotic enteritis in broiler chickens.

Broilers commonly suffer from necrotic enteritis (NE). Other gastrointestinal infectious diseases affect poultry, including nematode infections which are considered a re-emerging disease in barn and free-range systems. The aim of this study was to characterize the immune response of broilers after artificial infection with NE and contrast these with responses to the nematode Ascaridia galli and determine whether immune parameters measured during the course of infection can be used to distinguish infected from uninfected birds. A total of 96 one-day-old male Ross 308 broiler chickens were used in this study. At 10 days of age, broilers were randomly assigned to one of the following treatment groups: control birds (n = 32), A. galli infected birds (n = 32), or NE infected birds (n = 32) and inoculated with the appropriate infective agents. The immune response of birds was monitored through evaluation of haematology parameters, acute phase protein production, and intraepithelial intestinal lymphocyte population changes at 11, 16, 20, and 32 days of age. T-helper cells (CD4+CD8-) increased significantly over time, and were significantly higher in A. galli and NE compared to day 10 controls. In conclusion, α-1 glycoprotein levels can distinguish birds with NE from other birds, including those infected with A. galli; also T-helper cell numbers can distinguish both NE and A. galli from uninfected birds and thirdly, 10 days post infection is the best time point to evaluate the bird's immune response for A. galli infections.

Effect of dexmedetomidine on cerebral ischemia-reperfusion rats by activating mitochondrial ATP-sensitive potassium channel.

The aim of the study reported here was to evaluate whether the mitochondrial ATP-sensitive potassium (mitoKATP) channel could participate in the effect of dexmedetomidine on cerebral ischemia-reperfusion (I/R) rats. Forty rats were randomly assigned into 5 groups: sham operation (S) group; cerebral I/R group; dexmedetomidine (D) group; 5-hydroxydecanoate (5-HD) group; 5-HD + D group. The cerebral I/R were produced by 2 h right middle cerebral artery occlusion followed by 24 h reperfusion. Dexmedetomidine (50µg/kg) was injected intraperitoneally before ischemia and after the onset of reperfusion. 5-HD (30 mg/kg) was injected intraperitoneally at 1 h before ischemia. The neurological deficit score (NDS) and the levels of super oxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) were evaluated. Compared to group S, NDS and the levels of MDA, MPO, IL-6 and TNF-α were significantly higher, and SOD levels were significantly lower in the other groups (P < 0.05). Compared to group I/R,NDS and the levels of MDA, MPO, IL-6 and TNF-α were significantly lower, and SOD level was significantly higher in group D (P < 0.05). Compared to group D, NDS and the levels of MDA, MPO, IL-6 and TNF-α were significantly higher, and SOD level was significantly lower in group5-HD + D (P < 0.05). The activation of the mitoKATP channel could contribute to the protective effect of dexmedetomidine on rats induced by focal cerebral ischemia-reperfusion injury.

Phosphate recovery from liquid fraction of anaerobic digestate using four slow pyrolyzed biochars: Dynamics of adsorption, desorption and regeneration.

Four slow pyrolyzed biochars produced from wood (WDB), corncobs (CCB), rice husks (RHB) and sawdust (SDB) were evaluated for adsorption, desorption and regeneration of phosphate (PO43--P) from anaerobically digested liquid swine manure. The PO43--P adsorption capacity increased followed by initial concentrations increasing. Maximum PO43--P adsorptions at initial 150 mg/L of PO43--P (highest load) were average of 7.67, 6.43, 5.73 and 5.41 mg/g for WDB, CCB, RHB and SDB, respectively. Pseudo second order kinetics model could best fit PO43--P adsorption, which indicated the chemisorption via precipitation was the main mechanism for PO43--P removal. The sorption process was reversible and the adsorbed PO43--P could be desorbed in both neutral (57-78%) and acidic solution environments (75-88%) for all biochars. Meanwhile, regenerated biochar could re-adsorb up to 5.62 mg/g at the highest initial PO43--P of 150 mg/L. The present finding implied biochar could be effectively used to recover PO43--P from anaerobic digestate.

Growth and physiological responses of broiler chickens to diets containing raw, full-fat soybean and supplemented with a high-impact microbial protease.

OBJECTIVE: This study evaluated the change and function of the pancreas, and small intestine in relation to growth performance of broilers on diets supplemented with raw soybean meal (RSBM) and protease. Samples of test ingredients and diets, after mixing and prior to being used were also assessed on contents of anti-nutritional factors. METHODS: A 3×3 factorial study was used, with three levels of RSBM (commercial soybean meal [SBM] was replaced by RSBM at 0, 10%, or 20%) and protease (0.1, 0.2, or 0.3 g/kg). Each treatment was replicated six times with nine birds per replicate. Birds were housed in cages, in climate-controlled room and fed starter, grower and finisher diets. RESULTS: Levels of trypsin inhibitors in the diets, containing varying levels of RSBM ranged between 1,730.5 and 9,913.2 trypsin inhibitor units/g DM. Neither RSBM nor protease supplementation in diets significantly affected (p>0.05) the body weight of broilers in the entire periods (0 to 35-d). Increasing the level of RSBM in diets increased the weight of the pancreas at d 10 (p<0.000), d 24 (p<0.001), and d 35 (p<0.05). Increasing levels of RSBM in the diets reduced the apparent ileal digestibility of crude protein (CP), and amino acid (AA) at d 24. Increasing level of RSBM in the diets decreased (p<0.01) pancreatic protein content, but this was increased (p<0.05) when protease was added to the diets (0 to 10-d). Increasing the level of protease improved the pancreatic digestive enzymes, including trypsin (p<0.05), chymotrypsin (p<0.01), and general proteolytic enzymes (p<0.05). CONCLUSION: The commercial SBM could be replaced at up to 20% by RSBM for broilers. Although protease supplementation slightly improved the digestive enzymes, and the ileal digestibilities of CP and AA, the CP and AA were negatively affected by increasing RSBM.

Dynamics of intestinal metabolites and morphology in response to necrotic enteritis challenge in broiler chickens.

Despite the relatively small contribution to metabolizable energy that volatile fatty acids (VFAs) provide in chickens, these organic acids have been reported to play beneficial roles in the gastrointestinal tract (GIT) of birds, for example, inhibition of the growth of some pathogenic bacteria. However, information regarding the dynamics of these metabolites in the GIT of chickens is still scarce, especially under disease conditions such as necrotic enteritis (NE). Here, we investigated the dynamics of VFAs and lactic acid, and intestinal morphology in response to NE predisposing factors, that is, excessive dietary fishmeal and Eimeria inoculation, and causative agent Clostridium perfringens producing NetB toxin. The experiment was designed in a 2 × 2 × 2 factorial arrangement of treatments with or without: fishmeal feeding, Eimeria inoculation and C. perfringens challenge. The results showed that these factors significantly influenced composition and concentration of VFAs and lactic acids, pH and histomorphometry in one way or another. These changes may be important for the onset of NE or only the synergetic responses to micro environmental stress. Eimeria appeared to be more important than fishmeal in predisposing birds to NE, thus the application of Eimeria in NE challenge provides more consistent success in inducing the disease. The metabolic responses to various adverse factors such as excessive dietary fishmeal and Eimeria infection are complex. Thus, intensive efforts are required to better understand NE so as to achieve the control of the disease in the absence of antibiotics.

Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in Ziziphus jujuba.

Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of Ziziphus jujuba. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in Z. jujuba within 24 h upon Cd exposure. Escherichia coli cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and E. coli cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater.