ABSTRACT
Seawater intrusion is a complex groundwater - seawater interaction process, and it is influenced by many factors from ground surface to underground, from groundwater to seawater. Generally, for seawater intrusion model, some model parameters and boundary conditions are always specified by model users' personal experiences or literature's reference value. The defective model would damage the groundwater management for controlling and preventing seawater intrusion when making decisions are based on this model. In order to improve the reliability of seawater intrusion model, the influences of model inputs on output should be identified prior at optimizing model inputs. Dagu river basin, Jiaozhou Bay is one of the most serious areas of seawater intrusion in China, and it is chosen as the study area in this study. The seawater intrusion model of Dagu river basin is built based on a general program SEAWAT4. The key influence factors of model output are analyzed by two sensitivity analysis methods, i.e., stepwise regression and mutual entropy. The results demonstrated that the most important influence factors which have largest sensitivities to groundwater Cl- concentration are the precipitation rate and groundwater pumping in agriculture area. In addition, the hydraulic conductivity of zone 1 has a non-negligible influence on seawater intrusion process. Stepwise regression analysis is capable of identifying most important influence factor, and it can't handle complicated nonlinear input-output relationship. Mutual entropy analysis is reliable for identifying the influence factors for complex seawater intrusion model.
Subject(s)
Bays , Environmental Monitoring , Groundwater , Rivers , Seawater , China , Hydrology , Reproducibility of Results , Water MovementsABSTRACT
The particle size distribution characteristics of sediments and the concentrations of heavy metals in Jiaozhou Bay were investigated in this study. The average concentrations of Cu, Pb, Zn, Cr, Cd, Hg and As were 36.88, 29.60, 82.08, 77.48, 0.083, 0.048 and 11.00 mg/kg, respectively. The heavy metal concentrations were highest in the eastern sediments, followed by those at the top of the bay, and the lowest concentrations were observed in the central region. Overall, a decreasing trend from the center of the bay to the periphery was observed. Additionally, the distribution of heavy metals in sediments was not completely controlled by sediment particle size. The degree of heavy metal contamination was evaluated using the geoaccumulation index and Hakanson's method. The results revealed that the level of heavy metal pollution in the sediments was relatively low and that the main pollution elements were Cu and Hg. In addition, the sediments are associated with various levels of potential ecological risk due to the high pollution levels of Hg and Cd.
Subject(s)
Bays/chemistry , Environmental Monitoring/methods , Geologic Sediments/chemistry , Metals, Heavy/analysis , Water Pollutants, Chemical/analysis , China , Risk AssessmentABSTRACT
Pharmacotherapy of multidrug resistant (MDR) cancer remains a challenging task in clinic. Herein, a pH-responsive DNA and disulfide-linked polyethylenimine functionalized gold nanorod was developed for specific co-delivery of chemotherapeutic agent doxorubicin (DOX) and chemosensitizer pyronaridine (PND) to effectively overcome MDR cancer cells. DOX and PND were firstly carried by a multifunctional nanocomplex for reversing MDR cancer. The nanocomplex can responsively and rapidly release its drugs payload under acidic pH environment (pH, ~5), intracellular GSH concentration content (5 mM) and/or 808 nm NIR laser irradiation. Compared to free DOX, the nanocomplex displayed greatly increased cytotoxicity to MDR MCF-7/ADR cancer cells (IC50, 70.68:6.21 µg/mL). The application of NIR radiation further improved the DOX release and enhanced the antitumor effects of the namomedicine (IC50, drops to 2.88 µg/mL). Consequently, this new nanocomplex exerted greatly increased potency against the MDR cancer cells over free DOX (~20 fold).
Subject(s)
Antibiotics, Antineoplastic/administration & dosage , DNA , Doxorubicin/administration & dosage , Nanotubes , Antibiotics, Antineoplastic/pharmacology , Doxorubicin/pharmacology , Drug Carriers , Gold , Humans , Hydrogen-Ion Concentration , Oxidation-Reduction , Polyethyleneimine , Tumor Cells, CulturedABSTRACT
Microplastics ï¼MPsï¼ are a type of emerging contaminants that pose a potential threat to global terrestrial ecosystems. The accumulation of MPs in soil inevitably affects soil physical and chemical propertiesï¼ both directly and indirectly. Additionallyï¼ owing to their small size and surface featuresï¼ MPs have excellent sorption capacity for both organic and inorganic materialsï¼ thus affecting their fate in the environment. Howeverï¼ the influence of MPs on heavy metal sorption and transport in soil is still not fully understood. In this studyï¼ polyethylene ï¼PEï¼ and Cd were selected as research objectsï¼ and on the basis of clarifying the adsorption mechanism of Cd on PE MPsï¼ the effects of PE concentration and particle size on Cd release and transport behavior in soil under different ionic strengths and types ï¼Ca2+ and Na+ï¼ were studied using column leaching experiments. The results of the batch experiments showed that the adsorption capacity of PE MPs for Cd2+ decreased with the increase in particle size. Scanning electron microscopy ï¼SEMï¼ï¼ Fourier transform infrared spectroscopy ï¼FTIRï¼ï¼ and Zeta potential were used to analyze the properties of PE MPs and adsorption behavior of Cd2+ onto MPs. The adsorption was mainly a physical process and was controlled by intra-particle diffusion. The adsorption kinetics could be described well by the quasi-second-order kinetics and Webber-Morris model. The adsorption isotherm conformed to the Langmuir modelï¼ indicating monolayer adsorption. The results of leaching experiments showed that the effect of PE MPs on Cd release and transport in soil was related to the CaCl2 concentration. At high ionic strength ï¼0.05 mol·L-1 and 0.1 mol·L-1ï¼ï¼ PE promoted the transport of Cd. The effluent concentration of Cd2+ increased from 6.48 mg·L-1 and 16.79 mg·L-1 to 7.12 mg·L-1 and 23.45 mg·L-1ï¼ whereas at low ionic strength ï¼0.01 mol·L-1ï¼ï¼ Cd transport was inhibited by PE MPsï¼ and the effluent concentration of Cd2+ decreased from 0.66 mg·L-1 to 0.57 mg·L-1. The larger the amount of PE addedï¼ the more significant the promoting or inhibiting effect. Additionallyï¼ the release and transport of Cd in soil were also affected by the MPs particle size and concentration. When the addition amount was small ï¼1%ï¼ 4%ï¼ï¼ the large-sized MPs were more conducive to the transport of Cd in soil. When the addition amount was large ï¼7%ï¼ 20%ï¼ï¼ MPs with small particle sizes promoted Cd2+ transport more significantly. When the leaching solution used was NaClï¼ soil permeability decreased significantly. PE MPs had no significant effect on Cd release and transport but changed the stability of soil aggregates. In conclusionï¼ PE MPs could change the release and transport behavior of Cd in soilï¼ and the impact results were not only related to the particle size and content of MPs but were also influenced by the chemical properties of the soil solution.
ABSTRACT
Atomic engineering of the basal plane active sites in MoS2 holds great promise to boost the electrocatalytic activity for hydrogen evolution reactions (HER), yet the performance optimization and mechanism exploration are still not satisfactory. Herein, we proposed a dual-plasma engineering strategy to implant Ti and N heteroatoms into the basal plane of MoS2 supported by Ni3S2 nanorods on nickel foam (MSNF) for efficient electrocatalysis of HER. Owing to the low formation energy of Ti dopants in MoS2 and the extra charge carriers introduced by N dopants, the optimally codoped samples N1.0@Ti500-MSNF demonstrate significant morphology changes from nanorods to urchin-like nanospheres with the surface active areas increased by seven-fold, as well as enhanced electrical conductivity in comparison with the nondoped counterparts. The HER performance of N1.0@Ti500-MSNF is comparable with the Pt-based catalyst: overpotential of 26 mV at 20 mA cm-2, Tafel slope of 35.6 mV dec-1, and long-term stability over 50 h. First-principles calculation reveals that N doping accelerates the dissociation of water molecules while Ti doping activates the adjacent S sites for hydrogen adsorption by lowering the Gibbs free energy, resulting in excellent HER activity. This work thus provides an effective strategy for basal plane engineering of MoS2 heterostructures toward high-performance HER and sustainable energy supply at reasonable costs.
ABSTRACT
Inexpensive and efficient catalysts are crucial to industrial adoption of the electrochemical hydrogen evolution reaction (HER) to produce hydrogen. Although two-dimensional (2D) MoS2 materials have large specific surface areas, the catalytic efficiency is normally low. In this work, Ag and other dopants are plasma-implanted into MoS2 to tailor the surface and interface to enhance the HER activity. The HER activty increases initially and then decreases with increasing dopant concentrations and implantation of Ag is observed to produce better results than Ti, Zr, Cr, N, and C. At a current density of 400 mA cm-2 , the overpotential of Ag500-MoS2 @Ni3 S2 /NF is 150 mV and the Tafel slope is 41.7 mV dec-1 . First-principles calculation and experimental results reveal that Ag has higher hydrogen adsorption activity than the other dopants and the recovered S sites on the basal plane caused by plasma doping facilitate water splitting. In the two-electrode overall water splitting system with Ag500-MoS2 @Ni3 S2 /NF, a small cell voltage of 1.47 V yields 10 mA cm-2 and very little degradation is observed after operation for 70 hours. The results reveal a flexible and controllable strategy to optimize the surface and interface of MoS2 boding well for hydrogen production by commercial water splitting.
ABSTRACT
Photodynamic therapy (PDT) is emerging as an efficient strategy to combat multidrug-resistant (MDR) cancer. However, the short half-life and limited diffusion of reactive oxygen species (ROS) undermine the therapeutic outcomes of this therapy. To address this issue, a tumor-targeting nanoplatform was developed to precisely deliver mitochondria- and endoplasmic reticulum (ER)-targeting PDT agents to desired sites for dual organelle-targeted PDT. The nanoplatform is constructed by functionalizing molybdenum disulfide (MoS2) nanoflakes with glucose-modified hyperbranched polyglycerol (hPG), and then loading the organelle-targeting PDT agents. The resultant nanoplatform Cy7.5-TG@GPM is demonstrated to mediate both greatly enhanced internalization within MDR cells and precise subcellular localization of PDT agents, facilitating in situ near-infrared (NIR)-triggered ROS generation for augmented PDT and reversal of MDR, causing impressive tumor shrinkage in a HeLa multidrug-resistant tumor mouse model. As revealed by mechanistic studies of the synergistic mitochondria- and ER-targeted PDT, ROS-induced ER stress not only activates the cytosine-cytosine-adenosine-adenosine thymidine/enhancer-binding protein homologous protein (CHOP) pro-apoptotic signaling pathway, but also cooperates with ROS-induced mitochondrial dysfunction to trigger cytochrome C release from the mitochondria and induce subsequent cell death. Furthermore, the mitochondrial dysfunction reduces ATP production and thereby contributes to the reversal of MDR. This nanoplatform, with its NIR-responsive properties and ability to target tumors and subcellular organelles, offers a promising strategy for effective MDR cancer therapy.
Subject(s)
Nanoparticles , Photochemotherapy , Mice , Animals , Reactive Oxygen Species/metabolism , Molybdenum , Glucose , Cell Line, Tumor , Adenosine , Cytosine , Photosensitizing Agents/therapeutic use , Photosensitizing Agents/pharmacology , Nanoparticles/chemistryABSTRACT
Sulfadiazine (SDZ) is an antibiotic frequently detected in soil and groundwater. The transport of SDZ in subsurface environment is a critical process affecting its retention in soil. To date, the effects of iron oxide and metal cation on the transport of SDZ remain largely unknown, so we investigated the transport properties of SDZ in cleaned and iron oxides coated quartz sand, as affected by the presence of conventional cations (Ca2+, Mg2+, K+, and Na+) and Cd2+ through column experiments and simulation. We found that iron oxide coating on sand surface inhibited the transport of SDZ, mainly due to hydrophobic effect, complexation, and electrostatic attraction. The inhibitory effect became more marked with increasing concentration of Cd2+. It favors the transport of Cd2+ due to the electrostatic repulsion between positively charged iron oxide and Cd2+. Ca2+ promoted the transport of SDZ in coated sand, while all the conventional cations had no effect on the transport of SDZ in cleaned sand. The increase in the concentration of Cd2+ favors the transport of SDZ in cleaned sand. However, in iron oxide coated sand, the influence of Cd2+ on the transport of SDZ was dependent on the concentration of Cd2+. At lower concentration of Cd2+ and by competition, the transport is favored. At high concentration, the transport is inhibited mainly due to the formation of ternary surface complexes. A convective-dispersive transport model was applied to simulate and interpret experimental data. Breakthrough curves fitted well with a one-site model (OSM), indicating that SDZ adsorption on the sand experiences reversible kinetic. A low level of KF values with nearly linear sorption isotherm shows high mobility of SDZ and a high potential risk of surface and groundwater contamination. However, such high mobility can be reduced by increasing the content of iron oxides in porous media.
ABSTRACT
We propose a stacked dual-band quantum well infrared photodetector (QWIP) integrated with a double-layer gold disk. Two 10-period quantum wells (QW) operating at different wavelengths are stacked together, and gold nano-disks are integrated on their respective surfaces. Numerical calculations by finite difference time domain (FDTD) showed that the best enhancement can be achieved at 13.2 and 11.0 µm. By integrating two metal disks, two plasmon microcavity structures can be formed with the substrate to excite localized surface plasmons (LSP) so that the vertically incident infrared light can be converted into electric field components perpendicular to the growth direction of the quantum well (EZ). The EZ electric field component can be enhanced up to 20 times compared to the incident light, and it is four times that of the traditional two-dimensional hole array (2DHA) grating. We calculated the enhancement factor and coupling efficiency of the device in the active region of the quantum well. The enhancement factor of the active region of the quantum well on the top layer remains above 25 at the wavelength of 13.2 µm, and the enhancement factor can reach a maximum of 45. Under this condition, the coupling efficiency of the device reaches 2800%. At the wavelength of 11.0 µm, the enhancement factor of the active region of the quantum well at the bottom is maintained above 6, and the maximum can reach about 16, and the coupling efficiency of the device reaches 800%. We also optimized the structural parameters and explored the influence of structural changes on the coupling efficiency. When the radius (r1, r2) of the two metal disks increases, the maximum coupling efficiency will be red-shifted as the wavelength increases. The double-layer gold disk structure we designed greatly enhances the infrared coupling of the two quantum well layers working at different wavelengths in the dual-band quantum well infrared photodetector. The structure we designed can be used in stacked dual-band quantum well infrared photodetectors, and the active regions of quantum wells working at two wavelengths can enhance the photoelectric coupling, and the enhancement effect is significant. Compared with the traditional optical coupling structure, the structure we proposed is simpler in process and has a more significant enhancement effect, which can meet the requirements of working in complex environments such as firefighting, night vision, and medical treatment.
ABSTRACT
Wheat is the main food crop in China while at the same time, heavy metals pose a significant threat to crop growth and food security. Many studies indicate that rhizospheric microorganism play an important role in regulating crop development and stress resistance. In this study, the variation in wheat root-associated microbial communities under copper pollution was studied using high-throughput sequencing. The microbial community structure and diversity among different wheat rhizocompartments were compared after sequencing of microbial communities in the bulk soil, rhizosphere, and endosphere of wheat under copper pollution in combination with pot-based experiments. The results showed that the microbial diversity of the endosphere was significantly lower than in the rhizosphere and bulk soil(P<0.001), indicating that root surfaces serve as a gateway for microorganisms to enter into the interior root environment, and play a role in filtering root colonization. Copper pollution significantly reduced the microbial diversity of the rhizosphere (P<0.05). In the bulk soil and endosphere environments, although copper pollution reduced microbial diversity in the corresponding rhizocompartment, the difference was not significant (P>0.05). Proteobacteria and Actinobacteria were the dominant bacteria groups in the rhizosphere and the bulk soil under copper pollution. In addition, microbes such as Bacillus, Pseudoxanthomonas, and Sphingomonas show strong stress resistance and can provide nutrients for plants.
Subject(s)
Microbiota , Triticum , China , Copper/toxicity , Plant Roots , Rhizosphere , Soil MicrobiologyABSTRACT
2D MoS2 has shown a great potential in biomedical applications, due to its superior loading capacity, photothermal property, and biodegradation. In this work, polyglycerol functionalized MoS2 nanosheets with photothermal and pH dual-stimuli responsive properties are used for the co-delivery of doxorubicin and chloroquine and treatment of multidrug-resistant HeLa (HeLa-R) cells. The polyglycerol functionalized MoS2 nanosheets with 80 nm average size show a high biocompatibility and loading efficiency (≈90%) for both drugs. The release of drugs from the nanosheets at pH 5.5 is significantly promoted by laser irradiation leading to efficient destruction of incubated HeLa-R cells. In vitro evaluation shows that the designed nanoplatform has a high ability to kill HeLa-R cells. Confocal experiments demonstrate that the synthesized drug delivery system enhances the cellular uptake of DOX via folic acid targeting ligand. Taking advantage of the combined properties including biocompatibility and targeting ability as well as high loading capacity and photothermal release, this multifunctional nanosystem is a promising candidate for anticancer therapy.
Subject(s)
Chloroquine/administration & dosage , Disulfides/administration & dosage , Doxorubicin/administration & dosage , Drug Resistance, Neoplasm/drug effects , Glycerol/administration & dosage , Molybdenum/administration & dosage , Nanostructures/administration & dosage , Neoplasms/drug therapy , Polymers/administration & dosage , HeLa Cells , Humans , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , Spectrophotometry, UltravioletABSTRACT
Flexible rechargeable Zn//Ni batteries are attractive owing to their high energy density, good safety, inexpensive cost, and simple manufacturing process. However, the effects of metal doping on the properties of Ni3S2 cathodes in Zn/Ni batteries are not well understood. Herein, a binder-free Ni3S2 electrode is doped with Zn and Co and the nanocomposite structures are prepared on nickel foam (named ZCNS/NF) by a simple two-step hydrothermal technique. The ZCNS/NF//Zn battery delivers excellent electrochemical performance such as a working voltage window can be as high as 2.05 V, a capacity of 2.3 mAh cm-2 at 12 mA cm-2, and 82% retention going through 2000 cycles at 20 mA cm-2. The battery has a maximum output area energy density of 1.8 mWh cm-2 (462 Wh kg-1) and a power density of 36.8 mW cm-2 (9.2 kW kg-1). In addition, the flexible battery remains operational while being bent at a large angle and even punctured. The high performance and robustness of the composite cathode suggest that the design principle and materials have large commercial potential in Ni//Zn batteries.
ABSTRACT
Interest in soil pollution by multiple heavy metals has been growing over the last decades. However, few experiments combining numerical analyses with solute transport in layered soil can be found in the literature. Here, the retention and fate of three coexisting metal ions, Cu, Cd, and Zn, in layered soils were investigated to evaluate soil co-contamination through batch and column experiments. Results showed high amounts of Cu adsorption and retention by soils, followed by Cd and Zn. The partial concentration of Zn in effluent was greater than the input from competition adsorption and the 'snow plow effect'. These findings indicate the high potential risk of Zn and Cd groundwater pollution when Cu, Cd, and Zn co-exist in the soil. Adsorption isotherms obtained from batch experiments were well described by Freundlich equation. Breakthrough curves (BTCs) obtained from column experiments were well described by standard convection-dispersion equation (CDE) for Br, and Tow-site (TSM) and One-site models (OSM) for metals except for Zn, using the Levenberg-Marquardt nonlinear optimization algorithm. However, the parameters were poorly constrained by the available observational data due to high correlation between parameters, rather than insensitivity to model outputs. The Generalized Likelihood Uncertainty Estimation (GLUE) method did not only qualify the uncertainty of parameters for solute transport in layered medium, but estimate prediction uncertainty. Prediction bounds basically captured the observed Br, Zn and Cd BTCs, while systematically overestimated the effluent Cu concentration. Comparing with the optimization, GLUE method can improve prediction reliability of heavy metal transport in layered soils.
Subject(s)
Metals, Heavy/analysis , Soil Pollutants/analysis , Adsorption , Reproducibility of Results , SoilABSTRACT
INTRODUCTION: Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases. The development of NAFLD is closely associated with hepatic lipotoxicity, inflammation, and oxidative stress. The new concept of NAFLD treatment is to seek molecular control of lipid metabolism and hepatic redox hemostasis. Phoenixin is a newly identified neuropeptide with pleiotropic effects. This study investigated the effects of phoenixin 14 against high-fat diet (HFD)-induced NAFLD in mice. MATERIALS AND METHODS: For this study, we used HFD-induced NAFLD mice models to analyze the effect of phonenixin14. The mice were fed on HFD and normal diet and also given phoenixin 14 (100 ng/g body weight) by gastrogavage for 10 weeks. The peripheral blood samples were collected for biochemical assays. The liver tissues were examined for HFD-induced tissue fibrosis, lipid deposition and oxidative activity including SOD, GSH, and MDA. The liver tissues were analyzed for the inflammatory cytokines and oxidative stress pathway genes. RESULTS: The results indicate that phoenixin 14 significantly ameliorated HFD-induced obesity and fatty liver. The biochemical analysis of blood samples revealed that phoenixin 14 ameliorated HFD-induced elevated circulating alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol, and triglyceride levels, suggesting that phoenixin 14 has a protective role in liver function and lipid metabolism. Hematoxylin-eosin (HE) and Oil Red O staining of the liver showed that phoenixin 14 alleviated HFD-induced tissue damage and lipid deposition in the liver. Furthermore, the mice administered with phoenixin 14 had increased hepatic SOD activity, increased production of GSH and reduced MDA activity, as well as reduced production of TNF-α and IL-6 suggesting that phoenixin 14 exerts beneficial effects against inflammation and ROS. The findings suggest an explanation of how mechanistically phoenixin 14 ameliorated HFD-induced reduced activation of the SIRT1/AMPK and NRF2/HO-1 pathways. CONCLUSION: Collectively, this study revealed that phoenixin 14 exerts a protective effect in experimental NAFLD mice. Phoenixin could be of the interest in preventive modulation of NAFLD.
Subject(s)
Non-alcoholic Fatty Liver Disease/metabolism , Peptides/metabolism , Administration, Oral , Animals , Cytokines/analysis , Cytokines/metabolism , Diet, High-Fat , Disease Models, Animal , Fatty Liver/chemically induced , Fatty Liver/metabolism , Male , Mice , Mice, Inbred C57BL , Non-alcoholic Fatty Liver Disease/chemically induced , Obesity/chemically induced , Obesity/metabolism , Oxidative Stress , Peptides/administration & dosageABSTRACT
In the present study more than 1,000 structural parameters of 41 organophosphorus pesticides (OPs) were calculated using the software ChemOffice 8.03 and Dragon 2.1. Then, with multivariate linear regression and best subset regression analyses, different equations were derived to calculate the lethal toxicity, LC(50), for these 41 organophosphorous pesticides found in tadpoles (Bufo vulgaris formosus). An equation was developed for all selected OPs, especially those with relatively low toxicity levels (LC(50)>4.5mM) that accounted for 89.09% of the variability in the toxic effect. The equation indicated that the main contributions to OPs toxicity with tadpoles were the electrostatic contribution qH(+) (maximum net positive H atomic charge), spatial autocorrelation (MATS7 m) and hydrophobicity (lgK(ow)), with the two former being the most important parameters. For OPs with high toxicity, however, different structural parameters were introduced. The following equation was developed with LC(50)<4.5mM. These equations implied that with different levels of toxicity there could have different mechanisms in the tadpole. Furthermore, the results showed that molecular structural parameters had a particular value in modeling chemical reactivity within a homologous series of compounds.
Subject(s)
Bufonidae , Organophosphorus Compounds/toxicity , Pesticides/toxicity , Quantitative Structure-Activity Relationship , Animals , Larva/drug effects , Lethal Dose 50 , Models, Biological , Organophosphorus Compounds/chemistry , Pesticides/chemistryABSTRACT
Previously, combination chemotherapy of doxorubicin (DOX) and quercetin (QUR) was developed to improve antitumor effects and reverse multidrug resistance and several biocompatible nanocarriers, such as liposomes and micelles, were validated for their targeted delivery. In this study, we report a near-infrared (NIR)-responsive drug delivery system based on DOX and QUR co-loaded gold nanocages (AuNCs) with biotin modification. The system was simply fabricated by filling the hollow interiors of AuNCs with tetradecanol (TD), a phase-change material with a melting point of 39°C, to control the drug release. The main cause of multidrug resistance (MDR) of DOX is the overexpression of P-glycoprotein (P-gp), which can be inhibited by QUR. Thus the combination chemotherapy of DOX and QUR may provide a promising strategy for MDR. The in vitro cytotoxicity of DOX and QUR at several fixed mass ratios was carried out and showed that the combination index (CI) was the smallest at the ratio of 1:0.2, indicating that the best synergistic effect was achieved. The resultant nanocomplex (abbreviated as BPQD-AuNCs) exhibited fast release (80% released in 20min) and strong cytotoxicity against MCF-7/ADR cells (IC50, 1.5µg/mL) under NIR irradiation. Additionally, BPQD-AuNCs were found to generate a large amount of reactive oxygen species (ROS), to inhibit P-gp expression and ATP activity. Taken together, the results show that BPQD-AuNC is a prospective nano-delivery system for overcoming multidrug-resistant cancer.
Subject(s)
Antineoplastic Agents/chemistry , Doxorubicin/chemistry , Drug Carriers/chemistry , Fatty Alcohols/chemistry , Gold/chemistry , Metal Nanoparticles/chemistry , Quercetin/chemistry , ATP Binding Cassette Transporter, Subfamily B, Member 1/metabolism , Antineoplastic Agents/administration & dosage , Apoptosis , Biotin/chemistry , Cell Cycle , Cell Line, Tumor , Cell Survival , Doxorubicin/administration & dosage , Drug Liberation , Drug Resistance, Neoplasm , Humans , Light , Particle Size , Phototherapy , Quercetin/administration & dosage , Reactive Oxygen Species/metabolismABSTRACT
Pyrethroids are synthetic organic insecticides with low mammalian toxicity that are widely used in both rural and urban areas worldwide. After entering the natural environment, pyrethroids circulate among the three phases of solid, liquid, and gas and enter organisms through food chains, resulting in substantial health risks. This review summarized the available studies on pyrethroid residues since 1986 in different media at the global scale and indicated that pyrethroids have been widely detected in a range of environments (including soils, water, sediments, and indoors) and in organisms. The concentrations and detection rates of agricultural pyrethroids, which always contain α-cyanogroup (α-CN), such as cypermethrin and fenvalerate, decline in the order of crops > sediments > soils > water. Urban pyrethroids (not contain α-CN), such as permethrin, have been detected at high levels in the indoor environment, and 3-phenoxybenzoic acid, a common pyrethroid metabolite in human urine, is frequently detected in the human body. Pyrethroid pesticides accumulate in sediments, which are a source of pyrethroid residues in aquatic products.
Subject(s)
Environmental Pollutants/analysis , Pesticide Residues/analysis , Pyrethrins/analysis , Animals , Benzoates/urine , Humans , Insecticides/analysis , Nitriles/analysis , Permethrin/analysisABSTRACT
Effects of nanosized drug delivery systems on cancer are often compromised due to their low drug loadings, premature drug release and multi-drug resistance (MDR). Herein, we reported a glutathione detonated and pH responsive nano-cluster of Au nanorods (AuNRs) with chemotherapeutic doxorubicin (DOX) and pre-chemosensitizer polycurcumin to treat MCF-7/ADR cells. The nano-cluster was prepared by self-assembling of AuNRs conjugated with DOX and amphiphilic poly(curcumin-co-dithiodipropionic acid)-b-biotinylated poly(ethylene glycol) via an emulsion/solvent evaporation technique, termed AuNR Cluster. The AuNR Cluster had a high drug loading (31.5% DOX), presenting much better aqueous solubility and stability at physiological pH than their individual AuNRs. The AuNR Cluster could be detonated to be their individual AuNRs at an intracellular concentration level of glutathione (GSH) (5â¯mM) and triggered to release DOX at an acidic pH (pH 6.8 or 5.0), which effectively facilitated cellular uptake of DOX (607 vs 356 a.u. for AuNRs at 12â¯h) and inhibited DOX efflux (471.33 vs 39.17 a.u. for free DOX at 24â¯h). The IC50 value of DOX against MCF-7/ADR cells for AuNR Cluster was 4.15⯵g/mL, much lower than that for free DOX (90.97⯵g/mL). The AuNR Cluster took much more photothermal effects than their corresponding AuNRs and presented enhanced anti-tumor effect (IC50: 2.61⯵g/mL) under 808â¯nm laser irradiation. STATEMENT OF SIGNIFICANCE: Nano-sized drug delivery systems for anti-MDR cancer is still a challenging task. Herein, AuNR Cluster was self-assembled by individual AuNRs via emulsion/solvent evaporation technique, having a structure consisting of hydrophobic DOX/PCDA-AuNR core and hydrophilic biotin-PEG chain shell. AuNR Cluster is detonated to disintegrate and yield its individual AuNRs at an intracellular concentration level of glutathione (5â¯mM) and triggered to release DOX at an acidic pH (6.8 or 5.0). In comparison with its individual AuNRs, AuNR Cluster has better water solubility and stability, greater photothermal effects under NIR irradiation, bigger cytotoxicity against MCF-7/ADR cells. AuNR Cluster is expected to be a potential nanomedicine for treatment of MDR cancer.
Subject(s)
Doxorubicin , Drug Resistance, Multiple/drug effects , Drug Resistance, Neoplasm/drug effects , Glutathione/metabolism , Gold , Nanotubes/chemistry , Neoplasms , Delayed-Action Preparations/chemistry , Delayed-Action Preparations/pharmacokinetics , Delayed-Action Preparations/pharmacology , Doxorubicin/chemistry , Doxorubicin/pharmacokinetics , Doxorubicin/pharmacology , Female , Gold/chemistry , Gold/pharmacokinetics , Gold/pharmacology , Humans , Hydrogen-Ion Concentration , MCF-7 Cells , Neoplasms/drug therapy , Neoplasms/pathologyABSTRACT
To characterize air pollution and determine its source distribution in Qingdao, Shandong Province, we analyzed hourly national air quality monitoring network data of normal pollutants at nine sites from 1 November 2015 to 31 January 2016. The average hourly concentrations of particulate matter <2.5 µm (PM2.5) and <10 µm (PM10), SO2, NO2, 8-h O3, and CO in Qingdao were 83, 129, 39, 41, and 41 µg m-3, and 1.243 mg m-3, respectively. During the polluted period, 19-26 December 2015, 29 December 2015 to 4 January 2016, and 14-17 January 2016, the mean 24-h PM2.5 concentration was 168 µg m-3 with maximum of 311 µg m-3. PM2.5 was the main pollutant to contribute to the pollution during the above time. Heavier pollution and higher contributions of secondary formation to PM2.5 concentration were observed in December and January. Pollution pathways and source distribution were investigated using the HYbrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model and potential source contribution function (PSCF) and concentration weighted trajectory (CWT) analyses. A cluster from the west, originating in Shanxi, southern Hebei, and west Shandong Provinces, accounted for 44.1% of the total air masses, had a mean PM2.5 concentration of 134.9 µg m-3 and 73.9% trajectories polluted. This area contributed the most to PM2.5 and PM10 levels, >160 and 300 µg m-3, respectively. In addition, primary crustal aerosols from desert of Inner Mongolia, and coarse and fine marine aerosols from the Yellow Sea contributed to ambient PM. The ambient pollutant concentrations in Qingdao in winter could be attributed to local primary emissions (e.g., coal combustion, vehicular, domestic and industrial emissions), secondary formation, and long distance transmission of emissions.
Subject(s)
Air Pollutants/analysis , Seasons , Air Pollution/analysis , China , Coal/analysis , Environmental Monitoring , Particulate Matter/analysisABSTRACT
Because the surface of the cationic polymer gene complex is positively charged, it can result in problems such as poor blood stability and cytotoxicity. Therefore, reducing the positive charge of the cationic gene complex without affecting its transfection efficiency is crucial. To achieve this objective, a pH-responsive charge-convertible ternary complex was developed in this study. Modified plyethylenimine (PEI) with two different degrees of substitution of NH2 (plyethylenimine-1,2-cyclohexanedicarboxylic anhydride, PEI-CCA, and folic acid-plyethylenimine-1,2-cyclohexanedicarboxylic anhydride, FA-PEI-CCA) were first obtained by a chemical graft reaction. PEI-CCA and FA-PEI-CCA have significantly lower cytotoxicities and much better blood compatibilities than PEI does, and the former have an undifferentiated compression capability of DNA. The zeta potential values of the as-prepared ternary complexes (PEI-CCA/PEI/DNA and FA-PEI-CCA/PEI/DNA) were negative at pH 7.4 and positive at pH 6.5, with particle sizes of approximately 150nm. MTT assays demonstrated the significantly lower cytotoxicities of the ternary complexes compared to that of PEI/DNA. Moreover, the cytotoxicities of the ternary complexes were lower at pH 7.4 than pH 6.5. Transfection experiments in vitro revealed that the mean fluorescence intensities and transfection efficiencies of the ternary complexes were lower than for PEI/DNA at pH 7.4 but were almost the same at pH 6.5. The ternary complex with a FA group had significantly higher mean fluorescence intensity and transfection efficiency than did the ternary complex without it. In addition, the transfection experiment in 293T cells preliminarily validated the targeting function of the FA group of the ternary complex.