ABSTRACT
Counter-diffusional biofilms are efficient in the removal of nitrogen from low strength wastewaters. Although counter-diffusion is usually established using expensive gas-permeable membranes, a polyurethane sheet is used to separate the aerobic and anoxic environments in the novel foam aerated biofilm reactor (FABR). Foam sheets with thicknesses of 10, 5 and 2â mm and synthetic wastewater with COD/N ratios of 5 and 2.5 were evaluated. The 2â mm thick foam reactor did not show good biomass adherence and, therefore, did not show N removal efficiency. The 5 and 10â mm reactors, in both COD/N ratios, showed similar total nitrogen and COD removal performance, up to 60% and 80%, respectively. The denitrification efficiency was close to 100% throughout the experimental period. Nitrification efficiency decreased with microbial growth, which was recovered after removal of excessive biomass. Lower values of polyurethane foam thickness and COD/N ratio did not provide a higher nitrification rate, as expected. The increase in resistance to mass transfer was associated with the growth of biomass attached to the foam rather than to its thickness and resulted in specialization of the microbial communities as revealed by 16S amplicon sequencing. FABR reveals as a promising alternative for simultaneous removal of nitrogen and COD from low COD/N ratio wastewaters.
Subject(s)
Denitrification , Nitrogen , Biofilms , Bioreactors , Nitrification , WastewaterABSTRACT
The down flow structured bed bioreactor (DFSBR) was applied to treat synthetic acid mine drainage (AMD) to reduce sulfate, increase the pH and precipitate metals in solutions (Co, Cu, Fe, Mn, Ni and Zn) using vinasse as an electron donor for sulfate-reducing bacteria (SRB). DFSBR achieved sulfate removal efficiencies between 55 and 91%, removal of Co and Ni were obtained with efficiencies greater than 80%, while Fe, Zn, Cu and Mn were removed with average efficiencies of 70, 80, 73 and 60%, respectively. Sulfate reduction increased pH from moderately acidic to 6.7-7.5. Modelling data confirmed the experimental results and metal sulfide precipitation was the mainly responsible for metal removal. The main genera responsible for sulfate and metal reduction were Geobacter and Desulfovibrio while fermenters were Parabacteroides and Sulfurovum. Moreover, in syntrophism with SRB, they played an important role in the efficiency of metal and sulfate removal.