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1.
Cerebellum ; 23(5): 1942-1949, 2024 Oct.
Article in English | MEDLINE | ID: mdl-38607531

ABSTRACT

This was a study of 12 cerebellar cortical dysplasias (CCDs) fetuses, these cases were characterized by a disorder of cerebellar fissures. Historically, CCD diagnosis was primarily performed using postnatal imaging. Unique to this study was the case series of CCD for prenatal diagnosis using prenatal ultrasound, as well as we found that AXIN1 and FOXC1 mutations may be related to CCD.


Subject(s)
Prenatal Diagnosis , Humans , Female , Pregnancy , Prenatal Diagnosis/methods , Ultrasonography, Prenatal , Malformations of Cortical Development/diagnostic imaging , Malformations of Cortical Development/genetics , Adult , Forkhead Transcription Factors/genetics , Axin Protein/genetics , Cerebellar Cortex/diagnostic imaging , Cerebellar Cortex/abnormalities , Cerebellar Cortex/pathology , Mutation
2.
Anal Bioanal Chem ; 416(4): 849-859, 2024 Feb.
Article in English | MEDLINE | ID: mdl-38006441

ABSTRACT

Despite significant advancements in cancer research, real-time monitoring and effective treatment of cancer through non-invasive techniques remain a challenge. Herein, a novel polydopamine (PDA) nucleic acid nanoprobe has been developed for imaging signal amplification of intracellular mRNA and precise photothermal therapy guidance in cancer cells. The PDA nucleic acid nanoprobe (PDA@DNA) is constructed by assembling an aptamer hairpin (H1) labeled with the Cy5 fluorophore and another nucleic acid recognition hairpin (H2) onto PDA nanoparticles (PDA NPs), which have exceptionally high fluorescence quenching ability and excellent photothermal conversion properties. The nanoprobe could facilitate cellular uptake of DNA molecules and their protection from nuclease degradation. Upon recognition and binding to the intracellular mRNA target, a catalytic hairpin assembly (CHA) reaction occurs. The stem of H1 unfolds upon binding, allowing the exposed H1 to hybridize with H2, forming a flat and sturdy DNA double-stranded structure that detaches from the surface of PDA NPs. At the same time, the target mRNA is displaced and engages in a new cyclic reaction, resulting in the recovery and significant amplification of Cy5 fluorescence. Using thymidine kinase1 (TK1) mRNA as a model mRNA, this nanoprobe enables the analysis of TK1 mRNA with a detection limit of 9.34 pM, which is at least two orders of magnitude lower than that of a non-amplifying imaging nucleic acid probe. Moreover, with its outstanding performance for in vitro detection, this nanoprobe excels in precisely imaging tumor cells. Through live-cell TK1 mRNA imaging, it can accurately distinguish between tumor cells and normal cells. Furthermore, when exposed to 808-nm laser irradiation, the nanoprobe fully harnesses exceptional photothermal conversion properties of PDA NPs. This results in a localized temperature increase within tumor cells, which ultimately triggers apoptosis in these tumor cells. The integration of PDA@DNA presents innovative prospects for tumor diagnosis and image-guided tumor therapy, offering the potential for high-precision diagnosis and treatment of tumors.


Subject(s)
Carbocyanines , Indoles , Nanoparticles , Neoplasms , Polymers , Humans , Phototherapy , Photothermal Therapy , RNA, Messenger/chemistry , Nanoparticles/chemistry , DNA/chemistry , Neoplasms/pathology
3.
Int J Mol Sci ; 25(7)2024 Apr 05.
Article in English | MEDLINE | ID: mdl-38612877

ABSTRACT

Hedera helix is a traditional medicinal plant. Its primary active ingredients are oleanane-type saponins, which have extensive pharmacological effects such as gastric mucosal protection, autophagy regulation actions, and antiviral properties. However, the glycosylation-modifying enzymes responsible for catalyzing oleanane-type saponin biosynthesis remain unidentified. Through transcriptome, cluster analysis, and PSPG structural domain, this study preliminarily screened four candidate UDP-glycosyltransferases (UGTs), including Unigene26859, Unigene31717, CL11391.Contig2, and CL144.Contig9. In in vitro enzymatic reactions, it has been observed that Unigene26859 (HhUGT74AG11) has the ability to facilitate the conversion of oleanolic acid, resulting in the production of oleanolic acid 28-O-glucopyranosyl ester. Moreover, HhUGT74AG11 exhibits extensive substrate hybridity and specific stereoselectivity and can transfer glycosyl donors to the C-28 site of various oleanane-type triterpenoids (hederagenin and calenduloside E) and the C-7 site of flavonoids (tectorigenin). Cluster analysis found that HhUGT74AG11 is clustered together with functionally identified genes AeUGT74AG6, CaUGT74AG2, and PgUGT74AE2, further verifying the possible reason for HhUGT74AG11 catalyzing substrate generalization. In this study, a novel glycosyltransferase, HhUGT74AG11, was characterized that plays a role in oleanane-type saponins biosynthesis in H. helix, providing a theoretical basis for the production of rare and valuable triterpenoid saponins.


Subject(s)
Hedera , Oleanolic Acid/analogs & derivatives , Saponins , Glycosyltransferases/genetics
4.
Molecules ; 29(9)2024 Apr 26.
Article in English | MEDLINE | ID: mdl-38731485

ABSTRACT

Abnormal viscosity is closely related to the occurrence of many diseases, such as cancer. Therefore, real-time detection of changes in viscosity in living cells is of great importance. Fluorescent molecular rotors play a critical role in detecting changes in cellular viscosity. Developing red emission viscosity probes with large Stokes shifts and high sensitivity and specificity remains an urgent and important topic. Herein, a novel viscosity-sensitive fluorescent probe (TCF-VIS1) with a large stokes shift and red emission was prepared based on the 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) skeleton. Due to intramolecular rotation, the probe itself does not fluorescence at low viscosity. With the increase in viscosity, the rotation of TCF-VIS1 is limited, and its fluorescence is obviously enhanced. The probe has the advantages of simple preparation, large Stokes shift, good sensitivity and selectivity, and low cytotoxicity, which make it successfully used for viscosity detection in living cells. Moreover, TCF-VIS1 showed its potential for cancer diagnosis at the cell level and in tumor-bearing mice by detecting viscosity. Therefore, the probe is expected to enrich strategies for the detection of viscosity in biological systems and offer a potential tool for cancer diagnosis.


Subject(s)
Fluorescent Dyes , Animals , Fluorescent Dyes/chemistry , Viscosity , Mice , Humans , Cell Line, Tumor , Neoplasms/diagnosis , Neoplasms/pathology , Optical Imaging/methods
6.
Small ; 19(30): e2208270, 2023 Jul.
Article in English | MEDLINE | ID: mdl-37026657

ABSTRACT

It is not enough to develop an ideal hydrogen evolution reaction (HER) electrocatalysts by single strategy. Here, the HER performances are significantly improved by the combined strategies of P and Se binary vacancies and heterostructure engineering, which is rarely explored and remain unclear. As a result, the overpotentials of MoP/MoSe2 -H heterostructures rich in P and Se binary vacancies are 47 and 110 mV at 10 mA cm-2 in 1 m KOH and 0.5 m H2 SO4 electrolytes, respectively. Especially, in 1 m KOH, the overpotential of MoP/MoSe2 -H is very close to commercial Pt/C at the beginning and even better than Pt/C when current density is over 70 mA cm-2 . The strong interactions between MoSe2 and MoP facilitate electrons transfer from P to Se. Thus, MoP/MoSe2 -H possesses more electrochemically active sites and faster charge transfer capability, which are all in favor of high HER activities. Additionally, Zn-H2 O battery with MoP/MoSe2 -H as cathode is fabricated for simultaneous generation of hydrogen and electricity, which displays the maximum power density of up to 28.1 mW cm-2 and stable discharging performance for 125 h. Overall, this work validates a vigorous strategy and provides guidance for the development of efficient HER electrocatalysts.

7.
Mediators Inflamm ; 2023: 5380764, 2023.
Article in English | MEDLINE | ID: mdl-37181812

ABSTRACT

Background: Matrix metalloproteinase-7 (MMP7) is markedly expressed in patients with chronic kidney disease; its expression in dialysate and role in patients undergoing peritoneal dialysis (PD) have not been well established. Methods: Participants undergoing PD from June 1st, 2015, to June 30th, 2020, were involved and were followed up every 3 months for the first year and every 6 months thereafter until death, PD withdrawal, or the end of the study. Data at each follow-up point were collected and analyzed for the association with congestive heart failure (CHF), PD withdrawal, and combined endpoint. Results: A total of 283 participants were included in this study. During a median follow-up of 21 months, 20 (7%) participants died, 93 (33%) withdrew from PD, and 105 (37%) developed CHF. A significantly increased level of serum and dialysate MMP7 was observed at baseline. Dialysate MMP7 presented a good linearity with serum MMP7. Baseline serum and dialysate MMP7 levels were associated with CHF in multivariable Cox proportional hazards regression models. After categorization, participants with high baseline MMP7 levels had a higher incidence of CHF (42%), and the hazard ratios (95% confidence intervals) were 1.595 (1.023-2.488). Interestingly, participants with higher serum MMP7 levels were trended to use dialysate with higher glucose concentration. However, the ultrafiltration volumes were not significantly increased. Higher MMP7 levels were also positively associated with PD withdrawal and combined endpoint. Conclusions: The expression of MMP7 in serum and dialysate was markedly increased and was tightly associated with the risk of CHF in PD patients. This finding suggests that the measurement of MMP7 may inform strategies for managing CHF at an earlier stage.


Subject(s)
Heart Failure , Kidney Failure, Chronic , Peritoneal Dialysis , Humans , Matrix Metalloproteinase 7 , Prospective Studies , Kidney Failure, Chronic/therapy , Kidney Failure, Chronic/complications , Peritoneal Dialysis/adverse effects , Dialysis Solutions , Heart Failure/complications
8.
Ultraschall Med ; 43(6): e125-e134, 2022 Dec.
Article in English | MEDLINE | ID: mdl-33728625

ABSTRACT

PURPOSE: To describe the prenatal ultrasonographic characteristics and perinatal outcomes of congenital cataract. MATERIALS AND METHODS: We analyzed congenital cataract diagnosed prenatally at four referral centers between August 2004 and February 2019. The diagnosis was confirmed by postnatal ophthalmologic evaluation of liveborn infants or autopsy for terminated cases. Maternal demographics, genetic testing results, prenatal ultrasound images, and perinatal outcomes were abstracted. RESULTS: Total of 41 cases of congenital cataract diagnosed prenatally among 788 751 women undergoing anatomic survey. Based on the sonographic characteristics, 16/41 (39.0 %) had a dense echogenic structure, 15/41 (36.6 %) had a hyperechogenic spot and 10/41 (24.4 %) had the "double ring" sign. 17/41 (41.5 %) were isolated, and 24/41 (58.5 %) had associated intraocular and extraocular findings. Microphthalmia, cardiac abnormalities, and central nervous system abnormalities were the most common associated abnormalities. Regarding potential etiology, 6 cases had a known family history of congenital cataract, 4 cases had confirmed congenital rubella infection, and 2 cases had aneuploidy. 31/41 (75.6 %) elected termination and 10/41 (24.4 %) elected to continue their pregnancy. Among the 10 cases, one case died, one case was lost to follow-up, and the remaining 8 cases were referred for ophthalmologist follow-up and postnatal surgery. CONCLUSION: Once fetal cataracts are detected, a detailed fetal anatomy survey to rule out associated abnormalities and a workup to identify the potential etiology are recommended. Prenatal diagnosis of congenital cataracts provides vital information for counseling and subsequent management.


Subject(s)
Cataract , Fetal Diseases , Heart Defects, Congenital , Pregnancy , Female , Humans , Prenatal Diagnosis , Cataract/diagnostic imaging , Cataract/genetics , Heart Defects, Congenital/diagnostic imaging , Heart Defects, Congenital/genetics , Fetus , Ultrasonography, Prenatal , Retrospective Studies
9.
World J Microbiol Biotechnol ; 38(6): 110, 2022 May 12.
Article in English | MEDLINE | ID: mdl-35546212

ABSTRACT

Sanguinarine and chelerytrine have antibacterial and anti-inflammatory effects and is the main active ingredients of growth promoters in animals. Currently, Sanguinarine and chelerytrine were extracted from the capsules of the medicinal plant Macleaya cordata. However, the biomass of M. cordata nonmedicinal parts (leaves) accounted for a large proportion and contained a rich presentation of protopine and allocryptopine which are the precursor compounds of sanguinarine and chelerytrine. The aim of this study was to develop a new method for producing sanguinarine and chelerytrine through yeast transformation of protopine and allocryptopine in M. cordata leaves. First, we isolated different genes from Papaver somniferum (PsP6H, PsCPR, PsDBOX), Eschscholtzia californica (EcP6H), Cucumis sativus (CuCPR), Arabidopsis thaliana (AtCPR) and M. cordata (Mc11229, Mc11218, Mc6408, Mc6407, Mc19967, Mc13802). Additionally, some of the gene sequences were codon optimized. Then, we transformed these genes into yeast cells to compare the catalytic efficiency. Second, we used the most efficient strains to biotransform the leaves of M. cordata. Finally, we obtained 85.415 ± 11.887 ng mL-1 sanguinarine and 4.288 ± 1.395 ng mL-1 chelerytrine, which was more than 2-3 times the content in leaves of M. cordata. Overall, we using the nonmedicinal parts of M. cordata and successfully obtained sanguinarine and chelerytrine by the plant-microbial hybrid synthesis method.


Subject(s)
Alkaloids , Papaveraceae , Plants, Medicinal , Animals , Papaveraceae/genetics , Plant Leaves , Saccharomyces cerevisiae
10.
BMC Plant Biol ; 21(1): 125, 2021 Mar 01.
Article in English | MEDLINE | ID: mdl-33648452

ABSTRACT

BACKGROUND: Banana plant height is an important trait for horticultural practices and semi-dwarf cultivars show better resistance to damages by wind and rain. However, the molecular mechanisms controlling the pseudostem height remain poorly understood. Herein, we studied the molecular changes in the pseudostem of a semi-dwarf banana mutant Aifen No. 1 (Musa spp. Pisang Awak sub-group ABB) as compared to its wild-type dwarf cultivar using a combined transcriptome and metabolome approach. RESULTS: A total of 127 differentially expressed genes and 48 differentially accumulated metabolites were detected between the mutant and its wild type. Metabolites belonging to amino acid and its derivatives, flavonoids, lignans, coumarins, organic acids, and phenolic acids were up-regulated in the mutant. The transcriptome analysis showed the differential regulation of genes related to the gibberellin pathway, auxin transport, cell elongation, and cell wall modification. Based on the regulation of gibberellin and associated pathway-related genes, we discussed the involvement of gibberellins in pseudostem elongation in the mutant banana. Genes and metabolites associated with cell wall were explored and their involvement in cell extension is discussed. CONCLUSIONS: The results suggest that gibberellins and associated pathways are possibly developing the observed semi-dwarf pseudostem phenotype together with cell elongation and cell wall modification. The findings increase the understanding of the mechanisms underlying banana stem height and provide new clues for further dissection of specific gene functions.


Subject(s)
Musa/growth & development , Musa/genetics , Plant Stems/growth & development , Plant Stems/genetics , Cell Wall/genetics , Cell Wall/metabolism , Gibberellins/metabolism , Metabolome , Phenotype , Plant Growth Regulators/metabolism , Real-Time Polymerase Chain Reaction , Transcriptome
11.
BMC Pregnancy Childbirth ; 21(1): 148, 2021 Feb 18.
Article in English | MEDLINE | ID: mdl-33602166

ABSTRACT

BACKGROUND: In the entire population, an aberrant right subclavian artery (ARSA) is closely associated with chromosomal abnormalities. ARSA with additional ultrasonic findings would increase risk of chromosomal abnormalities. The risk of fetal chromosomal abnormalities increased exponentially with the maternal age. These risks in the advanced maternal age (AMA) group are uncertain. This study aimed to determine the incidence of ARSA in Chinese AMA and non-AMA women and the frequency of aneuploidy among AMA and non-AMA women with ARSA. METHODS: This retrospective study included 13,690 singleton pregnancies, were divided into AMA and non-AMA groups. Integrated obstetric ultrasonic screening, biochemical screening, noninvasive prenatal screening, and fetal karyotype analysis were analyzed. RESULTS: The overall incidence of ARSA was 0.69%, with no difference between age groups. The incidence of chromosomal abnormalities in the AMA group (37 / 2860) was much higher than that of the non-AMA group. The risk of chromosomal abnormalities significantly increased with both ARSA detected and additional ultrasound findings. With combined ARSA and AMA, the likelihood of the incidence of chromosomal abnormalities increased. Chimerism (45X / 46XX) was found with isolated ARSA in AMA pregnancies. CONCLUSION: There is a high prevalence of chromosomal abnormalities in fetuses of AMA women. ARSA increases the risk of chromosomal abnormalities in both age groups, especially combined with ARSA. When ARSA occurs in AMA women, it confers a high likelihood of chromosomal abnormalities.


Subject(s)
Aneuploidy , Cardiovascular Abnormalities/diagnostic imaging , Chromosome Aberrations , Subclavian Artery/abnormalities , Adult , Cardiovascular Abnormalities/epidemiology , Female , Humans , Incidence , Karyotyping , Maternal Age , Pregnancy , Pregnancy Trimester, Second , Retrospective Studies , Risk Factors , Subclavian Artery/diagnostic imaging , Ultrasonography, Prenatal , Young Adult
12.
Int J Clin Pract ; 75(8): e14188, 2021 Aug.
Article in English | MEDLINE | ID: mdl-33783932

ABSTRACT

BACKGROUND: Hypokalemia (LK) was associated with peritonitis in peritoneal dialysis (PD) patients, while the role of its degree and duration have not been fully established. Here, we conducted a retrospective cohort study to identify the relationships of LK degree and duration with peritonitis in PD patients. METHODS: A total of 602 PD patients in our department from Jan 1st, 2009 to Dec 31st, 2019 entered the last analysis. Data were collected from their medical records. Serum potassium (SK) levels, degree of hypokalemia, and duration of hypokalemia were analysed with peritonitis. The time association of hypokalemia and peritonitis was also analysed. RESULTS: There were totally 320 (53.7%) and 123 (20.7%) patients who had ever suffered from LK and serious hypokalemia (SLK) in the cohort. Only 6.82% and 0.5% of patients had LK and SLK at baseline, while the incidence increased and kept in 25%-32% and 5.5%-8.2% after PD. Both LK (HR 1.437, 95% CI 1.014-2.038, P = .042) and SLK (HR 2.021, 95% CI 1.429-2.857, P < .001) did correlate to peritonitis after adjusted analyses, while only SLK remained the significance at each follow-up point. The LK/SLK durations were 6 (3-12) and 6 (3-6) months, and only longer SLK duration correlated with peritonitis after adjusted analyses. After categorised, those LK durations more than 6 months and SLK durations more than 3 months presented a significant association with peritonitis. Of the patients who suffered from both hypokalemia and peritonitis, 70.4% patients' LK times were earlier than peritonitis time, while most SLK times (62.7%) were later. SLK also correlated with combined endpoint. CONCLUSIONS: Hypokalemia degree and duration were tightly associated with peritonitis. Hypokalemia might be a causal factor of peritonitis, while peritonitis might also aggravate hypokalemia. We should manage SK as much as possible and avoid hypokalemia, especially serious hypokalemia in clinic practice.


Subject(s)
Hypokalemia , Kidney Failure, Chronic , Peritoneal Dialysis , Peritonitis , Humans , Hypokalemia/complications , Peritoneal Dialysis/adverse effects , Peritonitis/epidemiology , Peritonitis/etiology , Potassium , Retrospective Studies , Risk Factors
13.
World J Surg Oncol ; 19(1): 141, 2021 May 05.
Article in English | MEDLINE | ID: mdl-33952287

ABSTRACT

BACKGROUND AND PURPOSE: With the advent of more intensive chemotherapy regimens, neoadjuvant chemoradiotherapy (NACRT) for patients with locally advanced rectal cancer (LARC) has always been questioned due to its inevitable radiation toxicity. Hence, we conducted a meta-analysis to compare the clinical efficacy of neoadjuvant chemotherapy (NAC) and NACRT. MATERIALS AND METHODS: Eligible studies were searched using PubMed, MEDLINE, Embase, the Cochrane Library, and Web of Science up to 31 July 2020, comparing the clinical efficacy of NAC versus NACRT for LARC. Short- and long-term outcomes were determined using the odds ratio (OR) with 95% confidence interval (CI). RESULTS: Six studies with 12,812 patients were eligible for this meta-analysis, including 677 patients in the NAC group and 12,135 patients in the NACRT group. There were no significant differences between the two groups in terms of pathological complete response rate (OR=0.62, 95%CI=0.27~1.41), N down-staging rate (OR=1.20, 95%CI=0.25~5.79), R0 resection rate (OR=1.24, 95%CI=0.78~1.98), and local relapse rate (OR=1.12, 95%CI=0.58~2.14). The pooled OR for the total response rate and T down-staging were in favor of NACRT (OR=0.41, 95%CI=0.22~0.76 versus OR=0.67 95%CI=0.52~0.87). However, the pooled OR for the sphincter preservation rate favored NAC compared with NACRT (OR=1.87, 95%CI=1.24~2.81). Moreover, NAC was found to be superior to NACRT in terms of distant metastasis (14.3% vs. 20.4%), but the difference was not significant (OR=0.84, 95%CI=0.31~2.27). CONCLUSION: We concluded that NAC was superior to NACRT in terms of the sphincter preservation rate, and non-inferior to NACRT in terms of pCR, N down-staging, R0 resection, local relapse, and distant metastasis. However, the conclusion warrants further validation.


Subject(s)
Neoadjuvant Therapy , Rectal Neoplasms , Chemoradiotherapy , Humans , Prognosis , Rectal Neoplasms/drug therapy , Treatment Outcome
14.
Molecules ; 26(13)2021 Jun 24.
Article in English | MEDLINE | ID: mdl-34202643

ABSTRACT

Bopu powder® and Sangrovit® were developed from Macleayacordata and are widely used in agriculture and animal husbandry, but their impurities have been rarely reported in the literature. Impurity analysis is of great importance to the quality and safety of veterinary drugs. In this study, high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) combined with a screening method was used to screen and characterize the impurities in Bopu powder® and Sangrovit®. A total of 58 impurities were screened from Bopu powder® and Sangrovit® using the screening strategies, of which 39 were identified by their accurate m/z value, characteristic MS/MS data, and fragmentation pathways of references. This established method was used for impurity analysis for the first time and proved to be a useful and rapid tool to screen and identify the impurities of Bopu powder® and Sangrovit®, especially for those at trace levels in a complex sample. In addition, this study marks the first comprehensive research into impurities in these two products and has great significance for the systematic detection of impurities in other plant-derived drugs.


Subject(s)
Drug Contamination , Drugs, Chinese Herbal/analysis , Chromatography, High Pressure Liquid , Powders , Spectrometry, Mass, Electrospray Ionization
15.
Nephrol Dial Transplant ; 34(5): 838-847, 2019 05 01.
Article in English | MEDLINE | ID: mdl-29733413

ABSTRACT

BACKGROUND: One of the major challenges in improving the management of antineutrophil cytoplasmic antibody-associated glomerulonephritis (ANCA-GN) is the lack of a disease-specific indicator for histological lesions and disease activity. Here we tested the utility of urinary angiotensinogen (UAGT) as a biomarker of renal disease activity in ANCA-GN. METHODS: A prospective, two-stage cohort study was performed in ANCA-GN patients. In Stage I, UAGT was measured at the time of renal biopsy in 69 patients from two centers (test set) and 25 patients from two other centers (validation set). In Stage II, UAGT was monitored in 50 subjects in the test set for 24 months. RESULTS: In Stage I, UAGT significantly increased in ANCA-GN patients, correlating well with cellular crescents formation and active interstitial inflammation. Patients with crescentic ANCA-GN exhibited the highest UAGT compared with other histopathological classes of ANCA-GN. After multivariable adjustment, the highest quartile of UAGT, compared with the lowest quartile, associated with a 6-fold increased risk of crescentic ANCA-GN. For predicting crescentic ANCA-GN, UAGT [area under the receiver operating characteristics curve (AUC) = 0.88] outperformed albuminuria (AUC = 0.73) and estimated glomerular filtration rate (AUC = 0.69). UAGT improved the performance of those clinical markers in diagnosing crescentic ANCA-GN (P < 0.034), suggesting a role of UAGT in identifying active crescentic ANCA-GN. In Stage II, UAGT decreased after immunotherapy and increased at the time of renal relapse during the 2-year follow-up, suggesting the usefulness of UAGT to monitor disease activity over time. CONCLUSIONS: These results suggest the potential use of UAGT for assessing disease activity and renal relapse in ANCA-GN.


Subject(s)
Angiotensinogen/urine , Antibodies, Antineutrophil Cytoplasmic/immunology , Glomerulonephritis/urine , Kidney/pathology , Adolescent , Adult , Aged , Aged, 80 and over , Biomarkers/urine , Biopsy , Female , Follow-Up Studies , Glomerulonephritis/immunology , Glomerulonephritis/pathology , Humans , Male , Middle Aged , Predictive Value of Tests , Prospective Studies , Young Adult
16.
Int J Mol Sci ; 20(3)2019 Jan 24.
Article in English | MEDLINE | ID: mdl-30678336

ABSTRACT

Four novel acylglycosides flavones (AGFs) including two quercetin acylglycosides and two kaempferol acylglycosides were isolated from Fuzhuan brick tea (FBT) as follows: quercetin 3-O-[α-l-rhamnopyranosyl (1→3)] [2-O''-(E)-p-coumaroyl] [ß-d-glucopyranosyl (1→3)-α-l-rhamnopyranosyl (1→6)]-ß-d-galactoside was named as camelliquercetiside E (1), quercetin 3-O-[α-l-rhamnopyranosyl (1→3)] [2-O''-(E)-p-coumaroyl] [α-l-rhamnopyranosyl (1→6)]-ß-d-galactoside was named as camelliquercetiside F (2), kaempferol 3-O-[α-l-arabinopyranosyl (1→3)] [2-O''-(E)-p-coumaroyl] [ß-d-glucopyranosyl (1→3)-α-l-rhamnopyranosyl (1→6)]-ß-d-glucoside was named as camellikaempferoside D (3), kaempferol 3-O-[α-l-arabinopyranosyl (1→3)] [2-O''-(E)-p-coumaroyl] [α-l-rhamnopyranosyl (1→6)]-ß-d-glucoside was named as camellikaempferoside E (4). Chemical structures of AGFs were identified by time-of-flight mass (TOF-MS) and NMR spectrometers (¹H NMR, 13C NMR, ¹H-¹H COSY, HMBC and HSQC), and the MS² fragmentation pathway of AGFs was further investigated. The inhibitory abilities of AGFs and their proposed metabolites on α-glucosidase and HMG-CoA reductase were analyzed by molecular docking simulation, and the results suggested that inhibitory activities of AGFs were significantly affected by acyl structure, number of glycosyl and conformation, and part of them had strong inhibitory activities on α-glucosidase and HMG-CoA reductase, suggesting that AGFs and their metabolites might be important ingredients that participate in the regulation of hypoglycemic and hypolipidemic effects. The results provided new AGFs and research directions for the practical study of FBT health functions in future.


Subject(s)
Kaempferols/pharmacology , Molecular Docking Simulation , Quercetin/pharmacology , Tea/chemistry , Acyl Coenzyme A/chemistry , Acyl Coenzyme A/metabolism , Camellia sinensis/chemistry , Glucosidases/chemistry , Glucosidases/metabolism , Glycosides/chemistry , Humans , Kaempferols/chemistry , Protein Binding , Quercetin/analogs & derivatives
17.
BMC Complement Altern Med ; 17(1): 511, 2017 Dec 01.
Article in English | MEDLINE | ID: mdl-29191190

ABSTRACT

BACKGROUND: Conventional scientific studies had supported the use of polysaccharides and ß-glucans from a number of fungi, including Ganoderma lucidum for the treatment of recurrent oral ulceration (ROU). Our aim of the present study was to evaluate whether freeze-dried powder from G. lucidum mycelia (FDPGLM) prevents ROU in rats. METHODS: A Sprague-Dawley (SD) rat model with ROU was established by autoantigen injection. The ROU rats were treated with three different dosages of FDPGLM and prednisone acetate (PA), and their effects were evaluated according to the clinical therapeutic evaluation indices of ROU. RESULTS: High-dose FDPGLM induced significantly prolonged total intervals and a reduction in the number of ulcers and ulcer areas, thereby indicating that the treatment was effective in preventing ROU. Enzyme-linked immunosorbent assay (ELISA) showed that high-dose FDPGLM significantly enhanced the serum transforming growth factor-ß1 (TGF-ß1) levels, whereas reduced those of interleukin-6 (IL-6) and interleukin-17 (IL-17). Flow cytometry (FCM) showed that the proportion of CD4+ CD25+ Foxp3+ (forkhead box P3) regulatory T cells (Tregs) significantly increased by 1.5-fold in the high-dose FDPGLM group compared to that in the rat model group (P < 0.01). The application of middle- and high-dose FDPGLM also resulted in the upregulation of Foxp3 and downregulation of retinoid-related orphan receptor gamma t(RORγt) mRNA. CONCLUSION: High-dose FDPGLM possibly plays a role in ROU by promoting CD4+ CD25+ Foxp3+ Treg and inhibiting T helper cell 17 differentiation. This study also shows that FDPGLM may be potentially used as a complementary and alternative medicine treatment scheme for ROU.


Subject(s)
Biological Products/therapeutic use , Ganoderma/chemistry , Mycelium/chemistry , Oral Ulcer/drug therapy , Animals , Biological Products/chemistry , Biological Products/pharmacology , Cytokines/blood , Disease Models, Animal , Forkhead Transcription Factors/genetics , Forkhead Transcription Factors/metabolism , Freeze Drying , Mouth Mucosa/chemistry , Mouth Mucosa/pathology , Prednisone/pharmacology , Prednisone/therapeutic use , Rats , Rats, Sprague-Dawley , Recurrence
18.
Prenat Diagn ; 35(2): 103-7, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25201035

ABSTRACT

OBJECTIVE: To describe the incidence, prenatal diagnosis, and perinatal outcome in fetuses with congenital dacryocystocele. METHODS: All cases of congenital dacryocystocele diagnosed by prenatal ultrasound were identified using an established perinatal database. Prenatal ultrasound images were reviewed, and perinatal outcome was abstracted from the medical records. The correlation between the cyst size, gestational age, and prenatal and neonatal outcome was analyzed. RESULTS: The overall incidence of fetal dacryocystocele was 0.016% (75/456,202). Fifty-three cases (70.7%) had unilateral, and 22 (29.3%) had bilateral lesions. Lesions were seen more commonly among female fetuses with a female : male ratio 1.48 : 1. The average diameter of cysts size was 6.9 ± 1.9 mm (3.3-11 mm). There was a direct correlation between cyst size and gestational age. Among the 75 cases, 8 were lost to follow-up, 3 underwent elective termination of pregnancy. 58 resolved spontaneously before birth, and 6 were confirmed at birth. Two developed neonatal infection requiring antibiotic treatment. All 6 cases confirmed at birth resolved without surgical resection. CONCLUSIONS: Congenital dacryocystocele can be diagnosed by prenatal ultrasound. Such lesions typically resolve spontaneously in utero or in the early neonatal period. Thus, it should be considered as a developmental variant rather than a structural birth defect.


Subject(s)
Cysts/diagnostic imaging , Fetal Diseases/diagnostic imaging , Lacrimal Duct Obstruction/complications , Adult , China/epidemiology , Cysts/congenital , Cysts/epidemiology , Female , Fetal Diseases/epidemiology , Fetal Diseases/etiology , Humans , Incidence , Lacrimal Duct Obstruction/congenital , Lacrimal Duct Obstruction/epidemiology , Pregnancy , Pregnancy Outcome , Retrospective Studies , Ultrasonography, Prenatal , Young Adult
19.
Fetal Pediatr Pathol ; 34(1): 57-64, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25289482

ABSTRACT

OBJECTIVE: To describe our 2 year experience in diagnosing prenatal-onset osteogenesis imperfecta (OI) by multidisciplinary assessment. METHODS: We retrospectively analyzed 10 cases of fetal OI by using prenatal ultrasound evaluation, postnatal radiographic diagnosis, and molecular genetic testing of COL1A1/2. RESULTS: By postnatal radiographic examination, five patients were diagnosed with type II OI and five were diagnosed with type III OI. A causative variant in the COL1A1 gene was found in four cases of type II and one case of type III OI; a causative variant in the COL1A2 gene was found in two cases of type III OI. CONCLUSION: The definitive diagnosis of fetal OI should be accomplished using a multidisciplinary assessment, which is paramount for proper genetic counseling. With the discovery of COL1A1/2 gene variants as a cause of OI, sequence analysis of these genes will add to the diagnostic process.


Subject(s)
Osteogenesis Imperfecta/diagnosis , Osteogenesis Imperfecta/embryology , Osteogenesis Imperfecta/genetics , Prenatal Diagnosis/methods , Adult , Collagen Type I/genetics , Collagen Type I, alpha 1 Chain , Female , Genetic Counseling , Genetic Variation , Humans , Molecular Biology , Pregnancy , Retrospective Studies , Ultrasonography, Prenatal , Young Adult
20.
Heliyon ; 10(7): e27262, 2024 Apr 15.
Article in English | MEDLINE | ID: mdl-38560234

ABSTRACT

With the advent of the era of media convergence, the storage management and inheritance of excellent traditional Chinese culture will usher in new opportunities, creating new opportunities for tapping the contemporary value of traditional culture. This work aims to explore the current protection and innovation of excellent traditional Chinese culture. It takes the digital storage and communication of intangible cultural heritage as an example to summarize the status quo of its storage management and communication, leading to the necessity of intelligent storage of excellent traditional culture. Meanwhile, in terms of intelligent storage management and communication, the application innovation of media convergence combined with blockchain technology has entered the public's vision. To better realize the intelligent storage and communication of excellent traditional Chinese culture, this work mainly studies how to operate the clever integration mechanism of media convergence and blockchain technology and summarizes the advantages of the two. The software development and design of the traditional culture storage system platform are carried out, and the platform's structural characteristics and development process are clarified. Finally, the mechanism is applied to the intelligent storage management and communication of traditional culture to test the time consumption of its network storage and information query. The results show that the test network has a good overall performance, short time consumption and stable operation when the storage network access is not blocked. This work can provide a more diversified information storage management and communication scheme, and provide a theoretical and practical basis for the intelligent storage management and communication of excellent traditional Chinese culture.

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