ABSTRACT
Previous studies suggested that the persistence in eukaryotic cells of a Bordetella bronchiseptica mutant carrying an insertion in the locus encoding the response regulator RisAS is impaired. This suggested that ris-dependent products are required for the intracellular survival of bacteria. In this study we demonstrate that ris-regulated products play a role in B. bronchiseptica resistance against both phagosomal acidification and reactive oxygen intermediates.
Subject(s)
Bacterial Proteins , Bordetella bronchiseptica/genetics , Bordetella bronchiseptica/physiology , Macrophages, Peritoneal/microbiology , Receptors, Cell Surface/metabolism , Adenosine Triphosphatases/metabolism , Animals , Anti-Bacterial Agents/pharmacology , Bordetella bronchiseptica/drug effects , Colony Count, Microbial , Female , Free Radical Scavengers/metabolism , Genes, Bacterial/physiology , Genes, Regulator/physiology , Macrolides , Macrophages, Peritoneal/enzymology , Macrophages, Peritoneal/metabolism , Mice , Mice, Inbred BALB C , Receptors, Cell Surface/geneticsABSTRACT
Using nucleic acid hybridization and polymerase chain reaction (PCR) HPV-DNA sequence was detected in endometrial and ovarian carcinomas. This sequence was amplified with primer specific for E1 region of DNA-HPV 18 and hybridized with DNA-HPV 18. The presence of HPV-DNA in cancer studies suggests that human papillomavirus can also be involved in carcinogenesis of the upper genital tract of women.
Subject(s)
DNA, Viral/analysis , Endometrial Neoplasms/virology , Ovarian Neoplasms/virology , Papillomaviridae/genetics , Adult , Aged , Female , Humans , Middle Aged , Nucleic Acid Hybridization , Polymerase Chain ReactionABSTRACT
OBJECTIVES: Much attention is given nowadays to the role of Human Papillomavirus, Herpes simplex virus, Cytomegalovirus, and Chlamydia trachomatis--infections in cervical carcinogenesis. Cytomegalovirus, Herpes simplex and Chlamydia trachomatis are now thought to be teratogenic to humans. DESIGN: We investigated the prevalence of HPV, HSV, CMV and Chlamydia trachomatis in genital tracts of sexual partners. MATERIALS AND METHODS: 90 sexual partners were qualified for the research. Examination smears were taken with the dacron swab from the vaginal part of the uterine cervix, cervical canal, the lower vagina from women and from fossa navicularis penis in men. In the group of 67 men we have investigated semen as well. HPV, HSV, CMV and Chlamydia trachomatis were identified using PCR (Polymerse Chain Reaction)--method. RESULTS AND CONCLUSIONS: In 48% of investigated sexual partners we proved the presence of Human Papillomavirus, in 2.2% of women and 2.9% of men--Cytomegalovirus and in 11.1% of women and 14.9% of men--Chlamydia trachomatis. In the investigated biological material we did not find any HSV infection.