Contrasting structures of plant-mite networks compounded by phytophagous and predatory mite species.

Differences in the feeding habits between phytophagous and predatory species can determine distinct ecological interactions between mites and their host plants. Herein, plant-mite networks were constructed using available literature on plant-dwelling mites from Brazilian natural vegetation in order to contrast phytophagous and predatory mite networks. The structural patterns of plant-mite networks were described through network specialization (connectance) and modularity. A total of 187 mite species, 65 host plant species and 646 interactions were recorded in 14 plant-mite networks. Phytophagous networks included 96 mite species, 61 host plants and 277 interactions, whereas predatory networks contained 91 mite species, 54 host plants and 369 interactions. No differences in the species richness of mites and host plants were observed between phytophagous and predatory networks. However, plant-mite networks composed of phytophagous mites showed lower connectance and higher modularity when compared to the predatory mite networks. The present results corroborate the hypothesis that trophic networks are more specialized than commensalistic networks, given that the phytophagous species must deal with plant defenses, in contrast to predatory mites which only inhabit and forage for resources on plants.

Global effects of land-use intensity and exotic plants on the structure and phylogenetic signal of plant-herbivore networks.

Interactions between plants and herbivorous insects are often phylogenetically structured, with closely related insect species using similar sets of species or lineages of plants, while phylogenetically closer plants tend to share high proportions of their herbivore insect species. Notably, these phylogenetic constraints in plant-herbivore interactions tend to be more pronounced among internal plant-feeding herbivores (i.e., endophages) than among external feeders (i.e., exophages). In the context of growing human-induced habitat conversion and the global proliferation of exotic species, it is crucial to understand how ecological networks respond to land-use intensification and the increasing presence of exotic plants. In this study, we analyzed plant-herbivore network data from various locations of the World to ascertain the degree to which land-use intensity and the prevalence of exotic plants induce predictable changes in their network topology - measured by levels of nestedness and modularity - and phylogenetic structures. Additionally, we investigated whether the intimacy of plant-herbivore interactions, contrasting endophagous with exophagous networks, modulate changes in network structure. Our findings reveal that most plant-herbivore networks are characterized by significant phylogenetic and topological structures. However, neither these structures did not show consistent changes in response to increased levels of land-use intensify. On the other hand, for the networks composed of endophagous herbivores, the level of nestedness was higher in the presence of a high proportion of exotic plants. Additionally, for networks of exophagous herbivores, we observed an increase in the phylogenetic structure of interactions due to exotic host dominance. These results underscore the differential impacts of exotic species and land-use intensity on the phylogenetic and topological structures of plant-herbivore networks.

Leishmania species infection of bats: A systematic review.

A wide variety of mammals, including domestic and wild species, have been considered potential hosts and reservoirs for Leishmania. Bats have longevity, dispersal capacity, and adaptability to synotropic environments, characteristics that may favor their role in maintaining the life cycle of parasites. Therefore, the objective of this study was to carry out a worldwide systematic review of the occurrence of Leishmania species in bats, as well as to identify associations between eating habits and the type of sample collected with the occurrence of the infection. Data were obtained from a bibliographic search for studies that used molecular methods to identify parasites, employing the keywords "bats" AND "Leishmania" and their synonyms. We found 68 original studies, of which 20 were included in this review. Most studies were conducted in Brazil (60 %) and only 10 % were conducted in Old World countries. In all, 48 bat species were recorded that hosted seven Leishmania species, resulting in 62 different host-parasite interactions, and the Leishmania infantum interaction with bat species presented higher frequency. There was no significant difference between Leishmania species richness, infection percentage, and type of sample analyzed, but in general, it is observed that the use of different biological samples seems to expand the possibility of parasite detection. The patterns observed here indicate that bats can become infected with a wide variety of Leishmania species and likely play an important role in maintaining the parasite's life cycle. Thus, we suggest that studies aimed at understanding the transmission cycle of leishmaniasis include the investigation of bats as potential hosts or reservoirs of Leishmania.

Leishmania diversity in bats from an endemic area for visceral and cutaneous leishmaniasis in Southeastern Brazil.

This study aimed to determine the occurrence of Leishmania infection in bats in urban and wild areas in an endemic municipality for visceral and cutaneous leishmaniasis in Minas Gerais, Brazil. Between April 2014 to April 2015, 247 bats were captured and classified into 26 species belonging to Phyllostomidae (90.7%), Vespertilionidae (8.1%) and Molossidae (1.2%) families. Blood samples from 247 bats were collected and submitted to nested-PCR, targeting the variable V7-V8 region of the SSU rRNA gene, followed by sequencing of the PCR product. The overall infection rate of Leishmania spp. in bats was 4.4%. Of the eleven bats infected, ten were frugivorous bats: Artibeus planirostris (8/11), Artibeus lituratus (1/11) and Artibeus cinereus (1/11) and one a nectarivorous bat (Glossophaga soricina). None of the individuals exhibited macroscopic alterations in the skin, spleen or liver. Phylogenetic analysis separated Leishmania species in clades corresponding to the subgenera Viannia, Leishmania, and Mundinia, and supported that the isolates characterized in the present study clustered closely with Leishmania (Viannia) sp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis. Here we report for the first time the bat Artibeus cinereus as a host of Leishmania (Leishmania) amazonensis. In the study we found that the mean abundance of bats did not differ in wild habitats and urban areas and that bat-parasite interactions were similarly distributed in the two environments. On the other hand, further studies should be conducted in more recent times to verify whether there have been changes in these parameters.

Geographical Patterns in the Architecture of Neotropical Flower-visitor Networks of Hummingbirds and Insects.

Geographical variations in environmental factors can affect species diversity and consequently influence the structure of interspecific ecological interactions. Relationships between flowering plants and animal flower visitors are among the most important ecological interactions and can structure and maintain ecological diversity in different environments. Additionally, many animal and plant species participate in these interactions, which shape the specific characteristics of these communities, in terms of both the responses of the interacting species involved and environmental differences. Therefore, in the present study we investigated geographical and environmental effects on the architecture of Neotropical flower-visitor networks of vertebrates and invertebrates. To this end, we used data regarding interaction networks available in the literature and constructed binary interaction networks of plants and plant-visitors (hummingbirds and insects) and tested the effects of altitude, latitude, vegetation type and number of plant families on the structure of these networks. In total, we analyzed 55 networks of flower-visitor interactions with 746 species of flower-visiting animals and 1,185 species of plants, totaling 5,463 distinct plant-animal interactions. In general, the architecture of flower-visitor networks varied along latitudinal and altitudinal gradients, with more pronounced effects for flower-insect networks in which latitude influenced network size, modularity, and nestedness, and altitude influenced network size and connectance. Flower-hummingbird networks in open vegetation (grassland) were more modular than networks in other environments. The number of plant families positively influenced the size of insect and hummingbird networks, and positively affected connectance and nestedness and negatively affected modularity in the flower-insect networks. So, the patterns we found indicate that plant-visitor interactions in flower-insect and flower-hummingbird networks are differently affected by geographical and plant-related factors, possibly due to the differences in taxonomic and functional groups involved in these interactions.

Diversity of Gall-Inducing Insects Associated With a Widely Distributed Tropical Tree Species: Testing the Environmental Stress Hypothesis.

Abiotic factors can affect plant performance and cause stress, which in turn affects plant-herbivore interactions. The Environmental Stress Hypothesis (ESH) predicts that gall-inducing insect diversity will be greater on host plants that grow in stressful habitats. We tested this hypothesis, considering both historical and ecological scales, using the plant Copaifera langsdorffii Desf. (Fabaceae) as a model because it has a wide geographic distribution and is a super-host of gall-inducing insects. According to the ESH, we predicted that 1) on a historical scale, the diversity of gall-inducing insects will be higher in habitats with greater environmental stress and 2) on an ecological scale, gall-inducing insect diversity will be greater on plants that possess greater levels of foliar sclerophylly. We sampled gall-inducing insects on plants of C. langsdorffii in five sites with different levels of water and soil nutrient availability and separated from each other by a distance of up to 470 km. The composition, richness, and abundance of gall-inducing insects varied among study sites. Plants located in more stressful habitats had higher levels of foliar sclerophylly; but richness and abundance of gall-inducing insects were not affected by host plant sclerophylly. Habitat stress was a good predictor of gall-inducing insect diversity on a regional scale, thus corroborating the first prediction of the ESH. No relationship was found between plant sclerophylly and gall-inducing insect diversity within habitats. Therefore, on a local scale, we did not find support for our second prediction related to the ESH.

Nocturnal Mosquitoes of Pará State in the Brazilian Amazon: Species Composition, Habitat Segregation, and Seasonal Variation.

Mosquitoes (Diptera: Culicidae) are one of the most important disease vector species in the world. Many species have a high degree of anthropophilia and are often found in human habitations. In the present study, we have inventoried the nocturnal mosquito assemblage in intra-, peri-, and extradomicile environments in four municipalities in Pará, Brazil. At each municipality, a residence was selected and the mosquitoes were sampled using the protected human attraction capture and Shannon trap methods in April (rainy season) and August 2018 (dry season). We have collected a total of 696 mosquito specimens belonging to 8 genera and 17 species. The most abundant species were Mansonia (Mansonoides) titillans (Walker) (366/696, 52.6%), Anopheles (Nyssorhynchus) albitarsis Lynch-Arribálzaga (97/696, 13.9%), and Culex (Culex) quinquefasciatus Say (93/696, 13.4%). Mosquito richness, abundance, and composition did not differ between intra-, peri-, and extradomicile environments suggesting limited habitat segregation among the different species. However, mosquito species richness and mosquito species abundance were significantly higher during the rainy season than during the dry season, suggesting increased mosquito activity during the rainy season. We detected several important vector species of human diseases including Aedes (Stegomyia) aegypti (Linnaeus), Anopheles (Nyssorhynchus) darlingi Root, Haemagogus (Conopostegus) leucocelaenus (Dyar and Shannon), Coquillettidia (Coquillettidia) venezuelensis (Theobald), and Culex (Culex) quinquefasciatus which are the main transmitters of dengue, malaria, yellow fever, mayaro, and oropouche fever, respectively. As inventories of disease-carrying mosquitoes in the region are very scarce, mainly in residential environments, our results suggest high potential for mosquito-borne disease transmission in Pará State.

Contrasting effects of land use intensity and exotic host plants on the specialization of interactions in plant-herbivore networks.

Human land use tends to decrease the diversity of native plant species and facilitate the invasion and establishment of exotic ones. Such changes in land use and plant community composition usually have negative impacts on the assemblages of native herbivorous insects. Highly specialized herbivores are expected to be especially sensitive to land use intensification and the presence of exotic plant species because they are neither capable of consuming alternative plant species of the native flora nor exotic plant species. Therefore, higher levels of land use intensity might reduce the proportion of highly specialized herbivores, which ultimately would lead to changes in the specialization of interactions in plant-herbivore networks. This study investigates the community-wide effects of land use intensity on the degree of specialization of 72 plant-herbivore networks, including effects mediated by the increase in the proportion of exotic plant species. Contrary to our expectation, the net effect of land use intensity on network specialization was positive. However, this positive effect of land use intensity was partially canceled by an opposite effect of the proportion of exotic plant species on network specialization. When we analyzed networks composed exclusively of endophagous herbivores separately from those composed exclusively of exophagous herbivores, we found that only endophages showed a consistent change in network specialization at higher land use levels. Altogether, these results indicate that land use intensity is an important ecological driver of network specialization, by way of reducing the local host range of herbivore guilds with highly specialized feeding habits. However, because the effect of land use intensity is offset by an opposite effect owing to the proportion of exotic host species, the net effect of land use in a given herbivore assemblage will likely depend on the extent of the replacement of native host species with exotic ones.

Checklist of host plants of insect galls in the state of Goiás in the Midwest Region of Brazil.

BACKGROUND: Surveys of host plants of insect galls have been performed in different regions of Brazil. The knowledge of species of host plants of insect galls is fundamental to further studies of plant-galling insect interactions. However, a list of host plant species of gall-inducing insects has not yet been compiled for the flora of the Midwest Region of Brazil. NEW INFORMATION: We provide a compilation of the plant species reported to host insect galls in the Cerrado of the state of Goiás in the Midwest Region of Brazil. Altogether we found records for 181 species of 47 families of host plants, which hosted 365 distinct gall morphotypes.