ABSTRACT
Vesicle-inducing protein in plastids 1 (VIPP1) is essential for the biogenesis and maintenance of thylakoid membranes, which transform light into life. However, it is unknown how VIPP1 performs its vital membrane-remodeling functions. Here, we use cryo-electron microscopy to determine structures of cyanobacterial VIPP1 rings, revealing how VIPP1 monomers flex and interweave to form basket-like assemblies of different symmetries. Three VIPP1 monomers together coordinate a non-canonical nucleotide binding pocket on one end of the ring. Inside the ring's lumen, amphipathic helices from each monomer align to form large hydrophobic columns, enabling VIPP1 to bind and curve membranes. In vivo mutations in these hydrophobic surfaces cause extreme thylakoid swelling under high light, indicating an essential role of VIPP1 lipid binding in resisting stress-induced damage. Using cryo-correlative light and electron microscopy (cryo-CLEM), we observe oligomeric VIPP1 coats encapsulating membrane tubules within the Chlamydomonas chloroplast. Our work provides a structural foundation for understanding how VIPP1 directs thylakoid biogenesis and maintenance.
Subject(s)
Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Chlamydomonas/metabolism , Protein Multimerization , Synechocystis/metabolism , Thylakoids/metabolism , Amino Acid Sequence , Bacterial Proteins/ultrastructure , Binding Sites , Cell Membrane/metabolism , Chlamydomonas/ultrastructure , Cryoelectron Microscopy , Green Fluorescent Proteins/metabolism , Hydrophobic and Hydrophilic Interactions , Light , Lipids/chemistry , Models, Molecular , Nucleotides/metabolism , Protein Binding , Protein Structure, Secondary , Stress, Physiological/radiation effects , Synechocystis/ultrastructure , Thylakoids/ultrastructureABSTRACT
The nexin-dynein regulatory complex (N-DRC) in motile cilia and flagella functions as a linker between neighboring doublet microtubules, acts to stabilize the axonemal core structure, and serves as a central hub for the regulation of ciliary motility. Although the N-DRC has been studied extensively using genetic, biochemical, and structural approaches, the precise arrangement of the 11 (or more) N-DRC subunits remains unknown. Here, using cryo-electron tomography, we have compared the structure of Chlamydomonas wild-type flagella to that of strains with specific DRC subunit deletions or rescued strains with tagged DRC subunits. Our results show that DRC7 is a central linker subunit that helps connect the N-DRC to the outer dynein arms. DRC11 is required for the assembly of DRC8, and DRC8/11 form a subcomplex in the proximal lobe of the linker domain that is required to form stable contacts to the neighboring B-tubule. Gold labeling of tagged subunits determines the precise locations of the previously ambiguous N terminus of DRC4 and C terminus of DRC5. DRC4 is now shown to contribute to the core scaffold of the N-DRC. Our results reveal the overall architecture of N-DRC, with the 3 subunits DRC1/2/4 forming a core complex that serves as the scaffold for the assembly of the "functional subunits," namely DRC3/5-8/11. These findings shed light on N-DRC assembly and its role in regulating flagellar beating.
Subject(s)
Chlamydomonas/metabolism , Dyneins/metabolism , Flagella/ultrastructure , Microtubule-Associated Proteins/metabolism , Chlamydomonas/genetics , Chlamydomonas/ultrastructure , Protein Structure, QuaternaryABSTRACT
In addition to bend propagation for swimming, Chlamydomonas cells use their flagella to glide along a surface. When polystyrene microspheres are added to cells, they attach to and move along the flagellar surface, thus serving as a proxy for gliding that can be used to assay for the flagellar components required for gliding motility. Gliding and microsphere movement are dependent on intraflagellar transport (IFT). Circumstantial evidence suggests that mechanical coupling of the IFT force-transducing machinery to a substrate is mediated by the flagellar transmembrane glycoprotein FMG-1B. Here, we show that cells carrying an insertion in the 5'-UTR of the FMG-1B gene lack FMG-1B protein, yet assemble normal-length flagella despite the loss of the major protein component of the flagellar membrane. Transmission electron microscopy shows a complete loss of the glycocalyx normally observed on the flagellar surface, suggesting it is composed of the ectodomains of FMG-1B molecules. Microsphere movements and gliding motility are also greatly reduced in the 5'-UTR mutant. Together, these data provide the first rigorous demonstration that FMG-1B is necessary for the normal expression of force at the flagellar surface in ChlamydomonasThis article has an associated First Person interview with authors from the paper.
Subject(s)
Chlamydomonas , Flagella , Glycoproteins , Plant Proteins , Chlamydomonas/genetics , Chlamydomonas/metabolism , Chlamydomonas/ultrastructure , Flagella/genetics , Flagella/metabolism , Flagella/ultrastructure , Glycoproteins/genetics , Glycoproteins/metabolism , Plant Proteins/genetics , Plant Proteins/metabolismABSTRACT
Many secreted peptides used for cell-cell communication require conversion of a C-terminal glycine to an amide for bioactivity. This reaction is catalyzed only by the integral membrane protein peptidylglycine α-amidating monooxygenase (PAM). PAM has been highly conserved and is found throughout the metazoa; PAM-like sequences are also present in choanoflagellates, filastereans, unicellular and colonial chlorophyte green algae, dinoflagellates and haptophytes. Recent studies have revealed that in addition to playing a key role in peptidergic signaling, PAM also regulates ciliogenesis in vertebrates, planaria and chlorophyte algae, and is required for the stability of actin-based microvilli. Here we briefly introduce the basic principles involved in ciliogenesis, the sequential reactions catalyzed by PAM and the trafficking of PAM through the secretory and endocytic pathways. We then discuss the multi-faceted roles this enzyme plays in the formation and maintenance of cytoskeleton-based cellular protrusions and propose models for how PAM protein and amidating activity might contribute to ciliogenesis. Finally, we consider why some ciliated organisms lack PAM, and discuss the potential ramifications of ciliary localized PAM for the endocrine features commonly observed in patients with ciliopathies.
Subject(s)
Chlamydomonas/enzymology , Cilia/metabolism , Mixed Function Oxygenases/metabolism , Multienzyme Complexes/metabolism , Peptides/metabolism , Plant Proteins/metabolism , Actins/metabolism , Chlamydomonas/cytology , Chlamydomonas/metabolism , Chlamydomonas/ultrastructure , Cilia/ultrastructure , Mixed Function Oxygenases/analysis , Models, Molecular , Multienzyme Complexes/analysis , Plant Proteins/analysis , Protein Biosynthesis , Protein Transport , Signal TransductionABSTRACT
Facing adverse conditions such as nitrogen (N) deprivation, microalgae enter cellular quiescence, a reversible cell cycle arrest with drastic changes in metabolism allowing cells to remain viable. Recovering from N deprivation and quiescence is an active and orderly process as we are showing here for Chlamydomonas reinhardtii We conducted comparative transcriptomics on this alga to discern processes relevant to quiescence in the context of N deprivation and recovery following refeeding. A mutant with slow recovery from N deprivation, compromised hydrolysis of triacylglycerols7 (cht7), was included to better define the regulatory processes governing the respective transitions. We identified an ordered set of biological processes with expression patterns that showed sequential reversal following N resupply and uncovered acclimation responses specific to the recovery phase. Biochemical assays and microscopy validated selected inferences made based on the transcriptional analyses. These comprise (1) the restoration of N source preference and cellular bioenergetics during the early stage of recovery; (2) flagellum-based motility in the mid to late stage of recovery; and (3) recovery phase-specific gene groups cooperating in the rapid replenishment of chloroplast proteins. In the cht7 mutant, a large number of programmed responses failed to readjust in a timely manner. Finally, evidence is provided for the involvement of the cAMP-protein kinase A pathway in gating the recovery. We conclude that the recovery from N deprivation represents not simply a reversal of processes directly following N deprivation, but a distinct cellular state.
Subject(s)
Chlamydomonas/genetics , Chlamydomonas/metabolism , Nitrogen/deficiency , Transcription, Genetic , Acclimatization , Cell Cycle , Chlamydomonas/ultrastructure , Cyclic AMP/metabolism , Cyclic AMP-Dependent Protein Kinases/metabolism , Galactolipids/metabolism , Gene Expression Profiling , Gene Expression Regulation , Lipid Metabolism/genetics , Metabolome/genetics , Mutation/genetics , Oxidation-Reduction , Sequence Analysis, RNA , Transcriptome/geneticsABSTRACT
Cilia and flagella often exhibit synchronized behavior; this includes phase locking, as seen in Chlamydomonas, and metachronal wave formation in the respiratory cilia of higher organisms. Since the observations by Gray and Rothschild of phase synchrony of nearby swimming spermatozoa, it has been a working hypothesis that synchrony arises from hydrodynamic interactions between beating filaments. Recent work on the dynamics of physically separated pairs of flagella isolated from the multicellular alga Volvox has shown that hydrodynamic coupling alone is sufficient to produce synchrony. However, the situation is more complex in unicellular organisms bearing few flagella. We show that flagella of Chlamydomonas mutants deficient in filamentary connections between basal bodies display markedly different synchronization from the wild type. We perform micromanipulation on configurations of flagella and conclude that a mechanism, internal to the cell, must provide an additional flagellar coupling. In naturally occurring species with 4, 8, or even 16 flagella, we find diverse symmetries of basal body positioning and of the flagellar apparatus that are coincident with specific gaits of flagellar actuation, suggesting that it is a competition between intracellular coupling and hydrodynamic interactions that ultimately determines the precise form of flagellar coordination in unicellular algae.
Subject(s)
Chlamydomonas/physiology , Flagella/physiology , Chlamydomonas/ultrastructure , Flagella/ultrastructure , Models, Biological , MovementABSTRACT
Intraflagellar transport (IFT) is responsible for the bidirectional trafficking of molecular components required for the elongation and maintenance of eukaryotic cilia and flagella. Cargo is transported by IFT 'trains', linear rows of multiprotein particles moved by molecular motors along the axonemal doublets. We have previously described two structurally distinct categories of 'long' and 'short' trains. Here, we analyse the relative number of these trains throughout flagellar regeneration and show that long trains are most abundant at the beginning of flagellar growth whereas short trains gradually increase in number as flagella elongate. These observations are incompatible with the previous hypothesis that short trains are derived solely from the reorganization of long trains at the flagellar tip. We demonstrate with electron tomography the existence of two distinct ultrastructural organizations for the short trains, we name these 'narrow' and 'wide', and provide the first 3D model of the narrow short trains. These trains are characterized by tri-lobed units, which repeat longitudinally every 16â nm and contact protofilament 7 of the B-tubule. Functional implications of the new structural evidence are discussed.
Subject(s)
Chlamydomonas/growth & development , Flagella/ultrastructure , Regeneration/genetics , Axoneme/metabolism , Axoneme/ultrastructure , Biological Transport , Chlamydomonas/genetics , Chlamydomonas/ultrastructure , Cilia/genetics , Cilia/ultrastructure , Electron Microscope Tomography , Flagella/genetics , Protein TransportABSTRACT
Electron microscopy (EM) for biological samples, developed in the 1940-1950s, changed our conception about the architecture of eukaryotic cells. It was followed by a period where EM applied to cell biology had seemingly fallen asleep, even though new methods with important implications for modern EM were developed. Among these was the discovery that samples can be preserved by chemical fixation and most importantly by rapid freezing without the formation of crystalline ice, giving birth to the world of cryo-EM. The past 15-20 years are hallmarked by a tremendous interest in EM, driven by important technological advances. Cryo-EM, in particular, is now capable of revealing structures of proteins at a near-atomic resolution owing to improved sample preparation methods, microscopes and cameras. In this review, we focus on the challenges associated with the imaging of membranes by EM and give examples from the field of host-pathogen interactions, in particular of virus-infected cells. Despite the advantages of imaging membranes under native conditions in cryo-EM, conventional EM will remain an important complementary method, in particular if large volumes need to be imaged.
Subject(s)
Cell Membrane/ultrastructure , Chlamydomonas/ultrastructure , Cryoelectron Microscopy/methods , Optic Nerve/ultrastructure , Vaccinia virus/ultrastructure , Virion/ultrastructure , Acrylic Resins , Animals , Cell Membrane/virology , Cryoelectron Microscopy/history , Cryoelectron Microscopy/instrumentation , HeLa Cells , History, 20th Century , History, 21st Century , Host-Pathogen Interactions , Humans , Imaging, Three-Dimensional , Mice , VitrificationABSTRACT
While cryo-electron tomography (cryo-ET) can reveal biological structures in their native state within the cellular environment, it requires the production of high-quality frozen-hydrated sections that are thinner than 300nm. Sample requirements are even more stringent for the visualization of membrane-bound protein complexes within dense cellular regions. Focused ion beam (FIB) sample preparation for transmission electron microscopy (TEM) is a well-established technique in material science, but there are only few examples of biological samples exhibiting sufficient quality for high-resolution in situ investigation by cryo-ET. In this work, we present a comprehensive description of a cryo-sample preparation workflow incorporating additional conductive-coating procedures. These coating steps eliminate the adverse effects of sample charging on imaging with the Volta phase plate, allowing data acquisition with improved contrast. We discuss optimized FIB milling strategies adapted from material science and each critical step required to produce homogeneously thin, non-charging FIB lamellas that make large areas of unperturbed HeLa and Chlamydomonas cells accessible for cryo-ET at molecular resolution.
Subject(s)
Frozen Sections/methods , Membrane Proteins/ultrastructure , Specimen Handling/methods , Chlamydomonas/ultrastructure , Electron Microscope Tomography/methods , HeLa Cells , Humans , Microscopy, Electron, Transmission/methodsABSTRACT
The confinement of Rubisco in a chloroplast microcompartment, or pyrenoid, is a distinctive feature of most microalgae, and contributes to perhaps ~30 Pg of carbon fixed each year, yet our understanding of pyrenoid composition, regulation, and function remains fragmentary. Recently, significant progress in understanding the pyrenoid has arisen from studies using mutant lines, mass spectrometric analysis of isolated pyrenoids, and advanced ultrastructural imaging of the microcompartment in the model alga Chlamydomonas. The emergence of molecular details in other lineages provides a comparative framework for this review, and evidence that most pyrenoids function similarly, even in the absence of a common ancestry. The objective of this review is to explore pyrenoid diversity throughout key algal lineages and discuss whether common ultrastructural and cellular features are indicative of common functional processes. By characterizing pyrenoid origins in terms of mechanistic and structural parallels, we hope to provide key unanswered questions which will inform future research directions.
Subject(s)
Chlamydomonas , Chloroplasts/metabolism , Chloroplasts/ultrastructure , Microalgae , Seaweed , Chlamydomonas/metabolism , Chlamydomonas/ultrastructure , Microalgae/metabolism , Microalgae/ultrastructure , Ribulose-Bisphosphate Carboxylase/metabolism , Seaweed/metabolism , Seaweed/ultrastructureABSTRACT
Microalgae have the potential to produce triacylglycerol (TAG) and starch, which provide alternative sources of biofuel. A problem in using Chlamydomonas reinhardtii as a model for TAG production has been that this alga lacks phosphatidylcholine (PC), which is thought to be important for TAG synthesis in plants. We found that C. debaryana is one of the rare species of Chlamydomonas having PC. Here we show that this strain, grown under complete photoautotrophic conditions, accumulated TAG and starch up to 20 and 250 pg per cell, respectively, during the stationary phase without nutrient deprivation. Addition of nutrients in this state did not cause loss of TAG, which was found in dilution with fresh medium. The photosynthetically produced TAG contained a high level of monounsaturated fatty acids, which is a preferred property as a material for biodiesel. The oil bodies were present in the cytoplasm, either between the cytoplasmic membrane and the chloroplast or between the chloroplast and the nucleus, whereas the starch granules were present within the chloroplast. Oil bodies were also deposited as a broad layer in the peripheral space of the cytoplasm outside the chloroplast, and might be easily released from the cells by genetic, chemical or mechanical manipulation. These results suggest that C. debaryana is a promising seed organism for developing a good biofuel producer.
Subject(s)
Autotrophic Processes , Chlamydomonas/growth & development , Chlamydomonas/metabolism , Starch/metabolism , Triglycerides/metabolism , Cell Proliferation , Chemical Fractionation , Chlamydomonas/ultrastructure , Fatty Acids/metabolism , Lipid Droplets/metabolism , Lipid Droplets/ultrastructureABSTRACT
In this paper, the use of lithium fluoride (LiF) as imaging radiation detector to analyse living cells by single-shot soft X-ray contact microscopy is presented. High resolved X-ray images on LiF of cyanobacterium Leptolyngbya VRUC135, two unicellular microalgae of the genus Chlamydomonas and mouse macrophage cells (line RAW 264.7) have been obtained utilizing X-ray radiation in the water window energy range from a laser plasma source. The used method is based on loading of the samples, the cell suspension, in a special holder where they are in close contact with a LiF crystal solid-state X-ray imaging detector. After exposure and sample removal, the images stored in LiF by the soft X-ray contact microscopy technique are read by an optical microscope in fluorescence mode. The clear image of the mucilaginous sheath the structure of the filamentous Leptolyngbya and the visible nucleolus in the macrophage cells image, are noteworthiness results. The peculiarities of the used X-ray radiation and of the LiF imaging detector allow obtaining images in absorption contrast revealing the internal structures of the investigated samples at high spatial resolution. Moreover, the wide dynamic range of the LiF imaging detector contributes to obtain high-quality images. In particular, we demonstrate that this peculiar characteristic of LiF detector allows enhancing the contrast and reveal details even when they were obscured by a nonuniform stray light.
Subject(s)
Fluorides , Lithium Compounds , Microscopy/methods , Animals , Chlamydomonas/ultrastructure , Cyanobacteria/ultrastructure , Lasers , Macrophages/ultrastructure , Mice , RAW 264.7 Cells , X-RaysABSTRACT
We cultured Chlamydomonas reinhardtii cells in a minimal culture medium supplemented with various concentrations of acetate, fatty acids, ethanol, fatty alcohols, or sucrose. The presence of acetate (0.5 or 1.0%, w/v) was advantageous for cell growth. To determine whether peroxisomes are involved in fatty acid and fatty alcohol metabolism, we investigated the dynamics of peroxisomes, including changes in their number and size, in the presence of acetate, ethanol, and sucrose. The total volume of peroxisomes increased when cells were grown with acetate, but did not change when cells were grown with ethanol or sucrose. We analyzed cell growth on minimal culture medium supplemented with various fatty acids (carbon chain length ranging from one to ten) to investigate which fatty acids are metabolized by C. reinhardtii. Among them, acetate caused the greatest increase in growth when added to minimal culture media. We analyzed the transcript levels of genes encoding putative glyoxysomal enzymes. The transcript levels of genes encoding malate synthase, malate dehydrogenase, isocitrate lyase, and citrate synthase increased when Chlamydomonas cells were grown on minimal culture medium supplemented with acetate. Our results suggest that Chlamydomonas peroxisomes are involved in acetate metabolism via the glyoxylate cycle.
Subject(s)
Acetates/pharmacology , Chlamydomonas/enzymology , Chlamydomonas/genetics , Gene Expression Regulation, Plant/drug effects , Glyoxysomes/enzymology , Peroxisomes/metabolism , Cell Proliferation/drug effects , Cells, Cultured , Chlamydomonas/cytology , Chlamydomonas/ultrastructure , Culture Media/pharmacology , Genes, Plant , Glyoxysomes/drug effects , Glyoxysomes/genetics , Microscopy, Fluorescence , Peroxisomes/drug effects , Peroxisomes/ultrastructure , RNA, Messenger/genetics , RNA, Messenger/metabolismABSTRACT
The pyrenoid is a subcellular microcompartment in which algae sequester the primary carboxylase, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). The pyrenoid is associated with a CO(2)-concentrating mechanism (CCM), which improves the operating efficiency of carbon assimilation and overcomes diffusive limitations in aquatic photosynthesis. Using the model alga Chlamydomonas reinhardtii, we show that pyrenoid formation, Rubisco aggregation, and CCM activity relate to discrete regions of the Rubisco small subunit (SSU). Specifically, pyrenoid occurrence was shown to be conditioned by the amino acid composition of two surface-exposed α-helices of the SSU: higher plant-like helices knock out the pyrenoid, whereas native algal helices establish a pyrenoid. We have also established that pyrenoid integrity was essential for the operation of an active CCM. With the algal CCM being functionally analogous to the terrestrial C(4) pathway in higher plants, such insights may offer a route toward transforming algal and higher plant productivity for the future.
Subject(s)
Chlamydomonas/enzymology , Organelles/metabolism , Ribulose-Bisphosphate Carboxylase/chemistry , Ribulose-Bisphosphate Carboxylase/metabolism , Amino Acid Sequence , Autotrophic Processes/drug effects , Carbon/metabolism , Carbon Dioxide/pharmacology , Chlamydomonas/drug effects , Chlamydomonas/growth & development , Chlamydomonas/ultrastructure , Gene Deletion , Kinetics , Molecular Sequence Data , Organelles/ultrastructure , Oxygen/metabolism , Phenotype , Photosynthesis/drug effects , Protein Structure, Secondary , Spinacia oleracea/drug effects , Spinacia oleracea/enzymology , Structure-Activity RelationshipABSTRACT
BACKGROUND: The versatile Vacuole Membrane Protein 1 (VMP1) has been previously investigated in six species. It has been shown to be essential in macroautophagy, where it takes part in autophagy initiation. In addition, VMP1 has been implicated in organellar biogenesis; endo-, exo- and phagocytosis, and protein secretion; apoptosis; and cell adhesion. These roles underly its proven involvement in pancreatitis, diabetes and cancer in humans. RESULTS: In this study we analyzed a VMP1 homologue from the green alga Chlamydomonas reinhardtii. CrVMP1 knockdown lines showed severe phenotypes, mainly affecting cell division as well as the morphology of cells and organelles. We also provide several pieces of evidence for its involvement in macroautophagy. CONCLUSION: Our study adds a novel role to VMP1's repertoire, namely the regulation of cytokinesis. Though the directness of the observed effects and the mechanisms underlying them remain to be defined, the protein's involvement in macroautophagy in Chlamydomonas, as found by us, suggests that CrVMP1 shares molecular characteristics with its animal and protist counterparts.
Subject(s)
Cell Shape , Chlamydomonas/cytology , Chlamydomonas/metabolism , Cytokinesis , Plant Proteins/metabolism , Amino Acid Sequence , Autophagy/genetics , Cell Cycle/genetics , Chlamydomonas/genetics , Chlamydomonas/ultrastructure , Chromatography, High Pressure Liquid , Gene Expression Regulation, Plant , Gene Knockdown Techniques , Genes, Plant , Mass Spectrometry , Metabolomics , Molecular Sequence Data , Mutation/genetics , Phenotype , Plant Proteins/chemistry , Principal Component Analysis , Proteolysis , Sequence AlignmentABSTRACT
The axoneme forms the essential and conserved core of cilia and flagella. We have used cryo-electron tomography of Chlamydomonas and sea urchin flagella to answer long-standing questions and to provide information about the structure of axonemal doublet microtubules (DMTs). Solving an ongoing controversy, we show that B-tubules of DMTs contain exactly 10 protofilaments (PFs) and that the inner junction (IJ) and outer junction between the A- and B-tubules are fundamentally different. The outer junction, crucial for the initiation of doublet formation, appears to be formed by close interactions between the tubulin subunits of three PFs with unusual tubulin interfaces; other investigators have reported that this junction is weakened by mutations affecting posttranslational modifications of tubulin. The IJ consists of an axially periodic ladder-like structure connecting tubulin PFs of the A- and B-tubules. The recently discovered microtubule inner proteins (MIPs) on the inside of the A- and B-tubules are more complex than previously thought. They are composed of alternating small and large subunits with periodicities of 16 and/or 48 nm. MIP3 forms arches connecting B-tubule PFs, contrary to an earlier report that MIP3 forms the IJ. Finally, the "beak" structures within the B-tubules of Chlamydomonas DMT1, DMT5, and DMT6 are clearly composed of a longitudinal band of proteins repeating with a periodicity of 16 nm. These findings, discussed in relation to genetic and biochemical data, provide a critical foundation for future work on the molecular assembly and stability of the axoneme, as well as its function in motility and sensory transduction.
Subject(s)
Axoneme/ultrastructure , Flagella/ultrastructure , Animals , Axoneme/chemistry , Chlamydomonas/chemistry , Chlamydomonas/genetics , Chlamydomonas/ultrastructure , Cryoelectron Microscopy , Electron Microscope Tomography , Flagella/chemistry , Imaging, Three-Dimensional , Male , Models, Molecular , Plant Proteins/chemistry , Protein Subunits , Sperm Tail/chemistry , Sperm Tail/ultrastructure , Strongylocentrotus purpuratus/chemistry , Strongylocentrotus purpuratus/ultrastructure , Tubulin/chemistryABSTRACT
Centriole duplication occurs once per cell cycle through the assembly of daughter centrioles on the side wall of pre-existing centrioles. Little is known about the molecules involved in the assembly of new centrioles. Here, we identify CRC70 as a Chlamydomonas protein with an important role in the accumulation of centriole proteins at the site of assembly. CRC70 contains a highly conserved ~50-amino-acid sequence shared by mammalian Cep70 and preferentially localizes to immature centrioles (the procentrioles). This localization is maintained in the mutant bld10, in which centriole formation is blocked before the assembly of centriolar microtubules. RNA interference (RNAi)-mediated knockdown of CRC70 produces flagella-less cells and inhibits the recruitment of other centriole components, such as SAS-6 and Bld10p to the centriole. Overexpression of CRC70 induces an accumulation of these proteins in discrete spots in the cytoplasm. Overexpression of EGFP-tagged CRC70 in mouse NIH3T3 cells causes the formation of structures apparently related to centrioles. These findings suggest that CRC70 is a member of a conserved protein family and functions as a scaffold for the assembly of the centriole precursor.
Subject(s)
Centrioles/physiology , Chlamydomonas/physiology , Microtubules/physiology , Plant Proteins/physiology , Amino Acid Sequence , Animals , Centrioles/genetics , Centrioles/metabolism , Chlamydomonas/genetics , Chlamydomonas/metabolism , Chlamydomonas/ultrastructure , Gene Knockdown Techniques , Gene Silencing , Mice , Microscopy, Electron , Microtubules/genetics , Microtubules/metabolism , Molecular Sequence Data , NIH 3T3 Cells , Plant Proteins/genetics , Plant Proteins/metabolism , RNA Interference , TransfectionABSTRACT
Although eukaryotic flagella and cilia all share the basic 9+2 microtubule-organization of their internal axonemes, and are capable of generating bending-motion, the waveforms, amplitudes, and velocities of the bending-motions are quite diverse. To explore the structural basis of this functional diversity of flagella and cilia, we here compare the axonemal structure of three different organisms with widely divergent bending-motions by electron cryo-tomography. We reconstruct the 3D structure of the axoneme of Tetrahymena cilia, and compare it with the axoneme of the flagellum of sea urchin sperm, as well as with the axoneme of Chlamydomonas flagella, which we analyzed previously. This comparative structural analysis defines the diversity of molecular architectures in these organisms, and forms the basis for future correlation with their different bending-motions.
Subject(s)
Chlamydomonas/ultrastructure , Cilia/ultrastructure , Flagella/ultrastructure , Sea Urchins/ultrastructure , Tetrahymena/ultrastructure , Animals , Axoneme/ultrastructure , Cryoelectron Microscopy , Electron Microscope Tomography , Image Processing, Computer-AssistedABSTRACT
There has been much interest in the chloroplast-encoded large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) as a target for engineering an increase in net CO(2) fixation in photosynthesis. Improvements in the enzyme would lead to an increase in the production of food, fiber, and renewable energy. Although the large subunit contains the active site, a family of rbcS nuclear genes encodes the Rubisco small subunits, which can also influence the carboxylation catalytic efficiency and CO(2)/O(2) specificity of the enzyme. To further define the role of the small subunit in Rubisco function, small subunits from spinach, Arabidopsis, and sunflower were assembled with algal large subunits by transformation of a Chlamydomonas reinhardtii mutant that lacks the rbcS gene family. Foreign rbcS cDNAs were successfully expressed in Chlamydomonas by fusing them to a Chlamydomonas rbcS transit peptide sequence engineered to contain rbcS introns. Although plant Rubisco generally has greater CO(2)/O(2) specificity but a lower carboxylation V(max) than Chlamydomonas Rubisco, the hybrid enzymes have 3-11% increases in CO(2)/O(2) specificity and retain near normal V(max) values. Thus, small subunits may make a significant contribution to the overall catalytic performance of Rubisco. Despite having normal amounts of catalytically proficient Rubisco, the hybrid mutant strains display reduced levels of photosynthetic growth and lack chloroplast pyrenoids. It appears that small subunits contain the structural elements responsible for targeting Rubisco to the algal pyrenoid, which is the site where CO(2) is concentrated for optimal photosynthesis.
Subject(s)
Algal Proteins/metabolism , Plant Proteins/metabolism , Recombinant Fusion Proteins/metabolism , Ribulose-Bisphosphate Carboxylase/metabolism , Algal Proteins/genetics , Arabidopsis/enzymology , Arabidopsis/genetics , Base Sequence , Blotting, Western , Chlamydomonas/genetics , Chlamydomonas/growth & development , Chlamydomonas/ultrastructure , DNA, Complementary/genetics , Enzyme Stability , Gene Expression Regulation, Enzymologic , Helianthus/enzymology , Helianthus/genetics , Kinetics , Microscopy, Electron , Molecular Sequence Data , Mutation , Photosynthesis , Plant Proteins/genetics , Plasmids/genetics , Protein Engineering/methods , Protein Subunits/genetics , Protein Subunits/metabolism , Recombinant Fusion Proteins/genetics , Ribulose-Bisphosphate Carboxylase/genetics , Temperature , Transformation, GeneticABSTRACT
Electron cryo-tomography is a potential approach to analyzing the three-dimensional conformation of frozen hydrated biological macromolecules using electron microscopy. Since projections of each individual object illuminated from different orientations are merged, electron tomography is capable of structural analysis of such heterogeneous environments as in vivo or with polymorphism, although radiation damage and the missing wedge are severe problems. Here, recent results on the structure of eukaryotic flagella, which is an ATP-driven bending organelle, from green algae Chlamydomonas are presented. Tomographic analysis reveals asymmetric molecular arrangements, especially that of the dynein motor proteins, in flagella, giving insight into the mechanism of planar asymmetric bending motion. Methodological challenges to obtaining higher-resolution structures from this technique are also discussed.