ABSTRACT
Coronavirus disease 2019 (COVID-19) exhibits variable symptom severity ranging from asymptomatic to life-threatening, yet the relationship between severity and the humoral immune response is poorly understood. We examined antibody responses in 113 COVID-19 patients and found that severe cases resulting in intubation or death exhibited increased inflammatory markers, lymphopenia, pro-inflammatory cytokines, and high anti-receptor binding domain (RBD) antibody levels. Although anti-RBD immunoglobulin G (IgG) levels generally correlated with neutralization titer, quantitation of neutralization potency revealed that high potency was a predictor of survival. In addition to neutralization of wild-type SARS-CoV-2, patient sera were also able to neutralize the recently emerged SARS-CoV-2 mutant D614G, suggesting cross-protection from reinfection by either strain. However, SARS-CoV-2 sera generally lacked cross-neutralization to a highly homologous pre-emergent bat coronavirus, WIV1-CoV, which has not yet crossed the species barrier. These results highlight the importance of neutralizing humoral immunity on disease progression and the need to develop broadly protective interventions to prevent future coronavirus pandemics.
Subject(s)
Antibodies, Neutralizing/immunology , Biomarkers/analysis , COVID-19/immunology , COVID-19/physiopathology , Adult , Antibodies, Neutralizing/analysis , Antibodies, Viral/analysis , Antibodies, Viral/blood , Biomarkers/blood , COVID-19/blood , COVID-19/epidemiology , Comorbidity , Coronavirus/classification , Coronavirus/physiology , Cross Reactions , Cytokines/blood , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunoglobulin A/analysis , Immunoglobulin G/blood , Immunoglobulin G/immunology , Immunoglobulin M/blood , Immunoglobulin M/immunology , Male , Massachusetts/epidemiology , Middle Aged , Protein Domains , SARS-CoV-2/chemistry , SARS-CoV-2/physiology , Severity of Illness Index , Spike Glycoprotein, Coronavirus/chemistry , Survival Analysis , Treatment OutcomeABSTRACT
Avoiding destruction by immune cells is a hallmark of cancer, yet how tumors ultimately evade control by natural killer (NK) cells remains incompletely defined. Using global transcriptomic and flow-cytometry analyses and genetically engineered mouse models, we identified the cytokine-TGF-ß-signaling-dependent conversion of NK cells (CD49a-CD49b+Eomes+) into intermediate type 1 innate lymphoid cell (intILC1) (CD49a+CD49b+Eomes+) populations and ILC1 (CD49a+CD49b-Eomesint) populations in the tumor microenvironment. Strikingly, intILC1s and ILC1s were unable to control local tumor growth and metastasis, whereas NK cells favored tumor immunosurveillance. Experiments with an antibody that neutralizes the cytokine TNF suggested that escape from the innate immune system was partially mediated by TNF-producing ILC1s. Our findings provide new insight into the plasticity of group 1 ILCs in the tumor microenvironment and suggest that the TGF-ß-driven conversion of NK cells into ILC1s is a previously unknown mechanism by which tumors escape surveillance by the innate immune system.
Subject(s)
Cellular Reprogramming/immunology , Fibrosarcoma/immunology , Gastrointestinal Neoplasms/immunology , Gastrointestinal Stromal Tumors/immunology , Immunity, Innate/immunology , Killer Cells, Natural/immunology , Neoplasms, Experimental/immunology , Tumor Escape/immunology , Animals , Case-Control Studies , Cell Line, Tumor , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Gene Expression Profiling , Humans , Killer Cells, Natural/cytology , Lymphocytes/cytology , Lymphocytes/immunology , Mice , Sequence Analysis, RNA , Signal Transduction , Transforming Growth Factor beta/immunologyABSTRACT
The balance of myeloid populations and lymphoid populations must be well controlled. Here we found that osteopontin (OPN) skewed this balance during pathogenic conditions such as infection and autoimmunity. Notably, two isoforms of OPN exerted distinct effects in shifting this balance through cell-type-specific regulation of apoptosis. Intracellular OPN (iOPN) diminished the population size of myeloid progenitor cells and myeloid cells, and secreted OPN (sOPN) increase the population size of lymphoid cells. The total effect of OPN on skewing the leukocyte population balance was observed as host sensitivity to early systemic infection with Candida albicans and T cell-mediated colitis. Our study suggests previously unknown detrimental roles for two OPN isoforms in causing the imbalance of leukocyte populations.
Subject(s)
Autoimmune Diseases/immunology , Candidiasis/immunology , Colitis/immunology , Infections/immunology , Lymphocytes/immunology , Myeloid Cells/immunology , Osteopontin/immunology , Animals , Apoptosis , Candida albicans , Cell Proliferation , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Lymphopoiesis/immunology , Mice , Mice, Knockout , Myelopoiesis/immunology , Osteopontin/genetics , Protein Isoforms , Real-Time Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction , T-LymphocytesABSTRACT
Aberrant population expansion of follicular helper T cells (TFH cells) occurs in patients with lupus. An unanswered question is whether an altered repertoire of T cell antigen receptors (TCRs) is associated with such expansion. Here we found that the transcription factor Blimp-1 (encoded by Prdm1) repressed expression of the gene encoding cathepsin S (Ctss), a cysteine protease that cleaves invariant chains and produces antigenic peptides for loading onto major histocompatibility complex (MHC) class II molecules. The increased CTSS expression in dendritic cells (DCs) from female mice with dendritic cell-specific conditional knockout of Prdm1 (CKO mice) altered the presentation of antigen to CD4+ T cells. Analysis of complementarity-determining region 3 (CDR3) regions containing the ß-chain variable region (Vß) demonstrated a more diverse repertoire of TFH cells from female CKO mice than of those from wild-type mice. In vivo treatment of CKO mice with a CTSS inhibitor abolished the lupus-related phenotype and reduced the diversity of the TFH cell TCR repertoire. Thus, Blimp-1 deficiency in DCs led to loss of appropriate regulation of Ctss expression in female mice and thereby modulated antigen presentation and the TFH cell repertoire to contribute to autoimmunity.
Subject(s)
Cathepsins/metabolism , Dendritic Cells/metabolism , Lupus Erythematosus, Systemic/immunology , T-Lymphocytes, Helper-Inducer/immunology , Transcription Factors/genetics , Animals , Antibodies, Antinuclear/immunology , Antigen Presentation/immunology , CD4-Positive T-Lymphocytes/immunology , Cell Differentiation , Cell Proliferation , DNA/immunology , Dendritic Cells/immunology , Enzyme-Linked Immunosorbent Assay , Female , Kidney/pathology , Lupus Erythematosus, Systemic/pathology , Lymphocyte Activation , Mice , Mice, Knockout , Positive Regulatory Domain I-Binding Factor 1 , Receptors, Antigen, T-Cell, alpha-beta/geneticsABSTRACT
Activated CD8+ T cells differentiate into cytotoxic effector (TEFF) cells that eliminate target cells. How TEFF cell identity is established and maintained is not fully understood. We found that Runx3 deficiency limited clonal expansion and impaired upregulation of cytotoxic molecules in TEFF cells. Runx3-deficient CD8+ TEFF cells aberrantly upregulated genes characteristic of follicular helper T (TFH) cell lineage, including Bcl6, Tcf7 and Cxcr5. Mechanistically, the Runx3-CBFß transcription factor complex deployed H3K27me3 to Bcl6 and Tcf7 genes to suppress the TFH program. Ablating Tcf7 in Runx3-deficient CD8+ TEFF cells prevented the upregulation of TFH genes and ameliorated their defective induction of cytotoxic genes. As such, Runx3-mediated Tcf7 repression coordinately enforced acquisition of cytotoxic functions and protected the cytotoxic lineage integrity by preventing TFH-lineage deviation.
Subject(s)
Core Binding Factor Alpha 3 Subunit/genetics , Lymphopoiesis/genetics , T-Lymphocytes, Cytotoxic/cytology , T-Lymphocytes, Helper-Inducer/cytology , Animals , Cell Lineage , Enzyme-Linked Immunosorbent Assay , Epigenesis, Genetic , Gene Expression Regulation , Hepatocyte Nuclear Factor 1-alpha/genetics , Immunohistochemistry , Mice , Proto-Oncogene Proteins c-bcl-6/genetics , Receptors, CXCR5/genetics , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, RNA , Up-RegulationABSTRACT
Infection with influenza virus induces antibodies to the viral surface glycoproteins hemagglutinin and neuraminidase, and these responses can be broadly protective. To assess the breadth and magnitude of antibody responses, we sequentially infected mice, guinea pigs and ferrets with divergent H1N1 or H3N2 subtypes of influenza virus. We measured antibody responses by ELISA of an extensive panel of recombinant glycoproteins representing the viral diversity in nature. Guinea pigs developed high titers of broadly cross-reactive antibodies; mice and ferrets exhibited narrower humoral responses. Then, we compared antibody responses after infection of humans with influenza virus H1N1 or H3N2 and found markedly broad responses and cogent evidence for 'original antigenic sin'. This work will inform the design of universal vaccines against influenza virus and can guide pandemic-preparedness efforts directed against emerging influenza viruses.
Subject(s)
Antibodies, Viral/immunology , Cross Reactions/immunology , Influenza A virus/immunology , Influenza, Human/immunology , Orthomyxoviridae Infections/immunology , Viral Envelope Proteins/immunology , Adolescent , Adult , Age Factors , Animals , Cluster Analysis , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Female , Ferrets , Guinea Pigs , Hemagglutinin Glycoproteins, Influenza Virus/immunology , Humans , Immunoglobulin G/immunology , Influenza A virus/classification , Male , Mice , Middle Aged , Neuraminidase/immunology , Viral Proteins/immunology , Young AdultABSTRACT
During infection, antigen-specific T cells undergo tightly regulated developmental transitions controlled by transcriptional and post-transcriptional regulation of gene expression. We found that the microRNA miR-31 was strongly induced by activation of the T cell antigen receptor (TCR) in a pathway involving calcium and activation of the transcription factor NFAT. During chronic infection with lymphocytic choriomeningitis virus (LCMV) clone 13, miR-31-deficent mice recovered from clinical disease, while wild-type mice continued to show signs of disease. This disease phenotype was explained by the presence of larger numbers of cytokine-secreting LCMV-specific CD8+ T cells in miR-31-deficent mice than in wild-type mice. Mechanistically, miR-31 increased the sensitivity of T cells to type I interferons, which interfered with effector T cell function and increased the expression of several proteins related to T cell dysfunction during chronic infection. These studies identify miR-31 as an important regulator of T cell exhaustion in chronic infection.
Subject(s)
Arenaviridae Infections/immunology , CD8-Positive T-Lymphocytes/immunology , Cytokines/immunology , MicroRNAs/immunology , Receptors, Antigen, T-Cell/immunology , Animals , Antibodies, Viral/immunology , Arenaviridae Infections/genetics , CD8-Positive T-Lymphocytes/drug effects , Calcium/metabolism , Chromatin Immunoprecipitation , Dendritic Cells/immunology , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Gene Expression Profiling , Immunoblotting , Interferon Type I/pharmacology , Lymphocytic choriomeningitis virus/immunology , Mice , Mice, Knockout , MicroRNAs/genetics , NFATC Transcription Factors/metabolism , Real-Time Polymerase Chain ReactionABSTRACT
RNA-binding proteins of the ZFP36 family are best known for inhibiting the expression of cytokines through binding to AU-rich elements in the 3' untranslated region and promoting mRNA decay. Here we identified an indispensable role for ZFP36L1 as the regulator of a post-transcriptional hub that determined the identity of marginal-zone B cells by promoting their proper localization and survival. ZFP36L1 controlled a gene-expression program related to signaling, cell adhesion and locomotion; it achieved this in part by limiting expression of the transcription factors KLF2 and IRF8, which are known to enforce the follicular B cell phenotype. These mechanisms emphasize the importance of integrating transcriptional and post-transcriptional processes by RNA-binding proteins for maintaining cellular identity among closely related cell types.
Subject(s)
B-Lymphocytes/immunology , Cell Adhesion/genetics , Cell Movement/genetics , Nuclear Proteins/genetics , RNA-Binding Proteins/genetics , Animals , Butyrate Response Factor 1 , Cell Adhesion/immunology , Cell Movement/immunology , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Fluorescent Antibody Technique , Gene Expression Regulation/genetics , High-Throughput Nucleotide Sequencing , Interferon Regulatory Factors/genetics , Kruppel-Like Transcription Factors/genetics , Lymphoid Tissue/cytology , Lymphoid Tissue/immunology , Mice , Nuclear Proteins/immunology , Phenotype , RNA-Binding Proteins/immunology , Real-Time Polymerase Chain Reaction , Sequence Analysis, RNA , Signal TransductionABSTRACT
Mammalian autophagy-related 8 (Atg8) homologs consist of LC3 proteins and GABARAPs, all of which are known to be involved in canonical autophagy. In contrast, the roles of Atg8 homologs in noncanonical autophagic processes are not fully understood. Here we show a unique role of GABARAPs, in particular gamma-aminobutyric acid (GABA)-A-receptor-associated protein-like 2 (Gabarapl2; also known as Gate-16), in interferon-γ (IFN-γ)-mediated antimicrobial responses. Cells that lacked GABARAPs but not LC3 proteins and mice that lacked Gate-16 alone were defective in the IFN-γ-induced clearance of vacuolar pathogens such as Toxoplasma. Gate-16 but not LC3b specifically associated with the small GTPase ADP-ribosylation factor 1 (Arf1) to mediate uniform distribution of interferon-inducible GTPases. The lack of GABARAPs reduced Arf1 activation, which led to formation of interferon-inducible GTPase-containing aggregates and hampered recruitment of interferon-inducible GTPases to vacuolar pathogens. Thus, GABARAPs are uniquely required for antimicrobial host defense through cytosolic distribution of interferon-inducible GTPases.
Subject(s)
ADP-Ribosylation Factor 1/immunology , Autophagy/immunology , Carrier Proteins/immunology , Interferon-gamma/immunology , Microtubule-Associated Proteins/immunology , Toxoplasma/immunology , Toxoplasmosis/immunology , ADP-Ribosylation Factor 1/metabolism , Animals , Apoptosis Regulatory Proteins , Autophagy-Related Protein 8 Family , CRISPR-Cas Systems , Carrier Proteins/metabolism , Computer Simulation , Cytoskeletal Proteins/immunology , Cytoskeletal Proteins/metabolism , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Fluorescent Antibody Technique , GTP Phosphohydrolases/immunology , GTP Phosphohydrolases/metabolism , Gene Editing , Immunoblotting , Immunoprecipitation , Interferon-gamma/metabolism , Intracellular Signaling Peptides and Proteins , Membrane Proteins/immunology , Membrane Proteins/metabolism , Mice , Microtubule-Associated Proteins/metabolismABSTRACT
Engineered crystallizable fragment (Fc) regions of antibody domains, which assume a unique and unprecedented asymmetric structure within the homodimeric Fc polypeptide, enable completely selective binding to the complement component C1q and activation of complement via the classical pathway without any concomitant engagement of the Fcγ receptor (FcγR). We used the engineered Fc domains to demonstrate in vitro and in mouse models that for therapeutic antibodies, complement-dependent cell-mediated cytotoxicity (CDCC) and complement-dependent cell-mediated phagocytosis (CDCP) by immunological effector molecules mediated the clearance of target cells with kinetics and efficacy comparable to those of the FcγR-dependent effector functions that are much better studied, while they circumvented certain adverse reactions associated with FcγR engagement. Collectively, our data highlight the importance of CDCC and CDCP in monoclonal-antibody function and provide an experimental approach for delineating the effect of complement-dependent effector-cell engagement in various therapeutic settings.
Subject(s)
Complement C1q/immunology , Cytotoxicity, Immunologic/immunology , Immunoglobulin Fc Fragments/immunology , Immunoglobulin G/immunology , Immunotherapy , Neoplasms/drug therapy , Phagocytosis/immunology , Receptors, IgG/immunology , Animals , Antibodies, Monoclonal , Burkitt Lymphoma/drug therapy , Burkitt Lymphoma/immunology , Cell Line, Tumor , Chromatography, Gel , Chromatography, Liquid , Complement C1q/metabolism , Crystallization , Crystallography, X-Ray , Enzyme-Linked Immunosorbent Assay , Humans , Immunoglobulin Fc Fragments/metabolism , Immunoglobulin G/metabolism , In Vitro Techniques , Lymphoma, B-Cell/drug therapy , Lymphoma, B-Cell/immunology , Lymphoma, Large B-Cell, Diffuse/drug therapy , Lymphoma, Large B-Cell, Diffuse/immunology , Mass Spectrometry , Mice , Neoplasms/immunology , Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy , Precursor Cell Lymphoblastic Leukemia-Lymphoma/immunology , Receptors, IgG/metabolism , Surface Plasmon Resonance , Tandem Mass SpectrometryABSTRACT
Viral respiratory tract infections are the main causative agents of the onset of infection-induced asthma and asthma exacerbations that remain mechanistically unexplained. Here we found that deficiency in signaling via type I interferon receptor led to deregulated activation of group 2 innate lymphoid cells (ILC2 cells) and infection-associated type 2 immunopathology. Type I interferons directly and negatively regulated mouse and human ILC2 cells in a manner dependent on the transcriptional activator ISGF3 that led to altered cytokine production, cell proliferation and increased cell death. In addition, interferon-γ (IFN-γ) and interleukin 27 (IL-27) altered ILC2 function dependent on the transcription factor STAT1. These results demonstrate that type I and type II interferons, together with IL-27, regulate ILC2 cells to restrict type 2 immunopathology.
Subject(s)
Immunity, Innate/immunology , Interferon Type I/immunology , Lymphocytes/immunology , Respiratory Tract Infections/immunology , Animals , Cytokines/biosynthesis , Cytokines/immunology , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Humans , Mice , Mice, Inbred C57BL , Mice, Knockout , Orthomyxoviridae Infections/immunology , Real-Time Polymerase Chain Reaction , Respiratory Tract Infections/pathologyABSTRACT
We used high-resolution mass spectrometry to map the cytotoxic T lymphocyte (CTL) proteome and the effect of the metabolic checkpoint kinase mTORC1 on CTLs. The CTL proteome was dominated by metabolic regulators and granzymes, and mTORC1 selectively repressed and promoted expression of a subset of CTL proteins (~10%). These included key CTL effector molecules, signaling proteins and a subset of metabolic enzymes. Proteomic data highlighted the potential for negative control of the production of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3) by mTORC1 in CTLs. mTORC1 repressed PtdIns(3,4,5)P3 production and determined the requirement for mTORC2 in activation of the kinase Akt. Our unbiased proteomic analysis thus provides comprehensive understanding of CTL identity and the control of CTL function by mTORC1.
Subject(s)
Multiprotein Complexes/metabolism , Proteome/immunology , T-Lymphocytes, Cytotoxic/metabolism , TOR Serine-Threonine Kinases/metabolism , Animals , Cells, Cultured , Chromatography , Enzyme-Linked Immunosorbent Assay , Female , Immunoblotting , Male , Mass Spectrometry , Mechanistic Target of Rapamycin Complex 1 , Mice , Multiprotein Complexes/immunology , Oligonucleotide Array Sequence Analysis , T-Lymphocytes, Cytotoxic/immunology , TOR Serine-Threonine Kinases/immunologyABSTRACT
The role of anergy, an acquired state of T cell functional unresponsiveness, in natural peripheral tolerance remains unclear. In this study, we found that anergy was selectively induced in fetal antigen-specific maternal CD4(+) T cells during pregnancy. A naturally occurring subpopulation of anergic polyclonal CD4(+) T cells, enriched for self antigen-specific T cell antigen receptors, was also present in healthy hosts. Neuropilin-1 expression in anergic conventional CD4(+) T cells was associated with hypomethylation of genes related to thymic regulatory T cells (Treg cells), and this correlated with their ability to differentiate into Foxp3(+) Treg cells that suppressed immunopathology. Thus, our data suggest that not only is anergy induction important in preventing autoimmunity but also it generates the precursors for peripheral Treg cell differentiation.
Subject(s)
Autoimmunity/immunology , Cell Differentiation/immunology , Clonal Anergy/immunology , Histocompatibility, Maternal-Fetal/immunology , Peripheral Tolerance/immunology , Precursor Cells, T-Lymphoid/immunology , T-Lymphocytes, Regulatory/immunology , Adoptive Transfer , Animals , Arthritis, Experimental/immunology , CD4-Positive T-Lymphocytes/immunology , Cell Proliferation , Cytokines/immunology , Enzyme-Linked Immunosorbent Assay , Female , Flow Cytometry , Forkhead Transcription Factors/immunology , Genes, T-Cell Receptor alpha , Immunoblotting , Male , Mice , Mice, Knockout , Neuropilin-1/metabolism , Pregnancy , Receptors, Antigen, T-Cell/immunology , Reverse Transcriptase Polymerase Chain Reaction , Self Tolerance , Thymocytes/immunologyABSTRACT
The signaling adaptor TRAF3 is a highly versatile regulator of both innate immunity and adaptive immunity, but how its phosphorylation is regulated is still unknown. Here we report that deficiency in or inhibition of the conserved serine-threonine kinase CK1É suppressed the production of type I interferon in response to viral infection. CK1É interacted with and phosphorylated TRAF3 at Ser349, which thereby promoted the Lys63 (K63)-linked ubiquitination of TRAF3 and subsequent recruitment of the kinase TBK1 to TRAF3. Consequently, CK1É-deficient mice were more susceptible to viral infection. Our findings establish CK1É as a regulator of antiviral innate immune responses and indicate a novel mechanism of immunoregulation that involves CK1É-mediated phosphorylation of TRAF3.
Subject(s)
Casein Kinase 1 epsilon/immunology , Immunity, Innate/immunology , Interferon-beta/immunology , TNF Receptor-Associated Factor 3/immunology , Animals , Casein Kinase 1 epsilon/antagonists & inhibitors , Casein Kinase 1 epsilon/genetics , Enzyme-Linked Immunosorbent Assay , HEK293 Cells , HeLa Cells , Herpes Simplex/immunology , Herpesvirus 1, Human/immunology , Humans , Interferon Type I/biosynthesis , Interferon Type I/immunology , Interferon-beta/biosynthesis , Mass Spectrometry , Mice , Mice, Knockout , Phosphorylation , Protein Serine-Threonine Kinases , Real-Time Polymerase Chain Reaction , Rhabdoviridae Infections/immunology , TNF Receptor-Associated Factor 3/genetics , Ubiquitination , Vesiculovirus/immunology , West Nile Fever/immunology , West Nile virus/immunologyABSTRACT
Plasma cell differentiation requires silencing of B cell transcription, while it establishes antibody-secretory function and long-term survival. The transcription factors Blimp-1 and IRF4 are essential for the generation of plasma cells; however, their function in mature plasma cells has remained elusive. We found that while IRF4 was essential for the survival of plasma cells, Blimp-1 was dispensable for this. Blimp-1-deficient plasma cells retained their transcriptional identity but lost the ability to secrete antibody. Blimp-1 regulated many components of the unfolded protein response (UPR), including XBP-1 and ATF6. The overlap in the functions of Blimp-1 and XBP-1 was restricted to that response, with Blimp-1 uniquely regulating activity of the kinase mTOR and the size of plasma cells. Thus, Blimp-1 was required for the unique physiological ability of plasma cells that enables the secretion of protective antibody.
Subject(s)
Cell Differentiation/immunology , Immunoglobulins/immunology , Interferon Regulatory Factors/immunology , Plasma Cells/immunology , Transcription Factors/immunology , Unfolded Protein Response/immunology , Activating Transcription Factor 6/genetics , Activating Transcription Factor 6/immunology , Animals , Cell Size , Chromatin Immunoprecipitation , DNA-Binding Proteins/genetics , DNA-Binding Proteins/immunology , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Gene Expression Profiling , Gene Expression Regulation , High-Throughput Nucleotide Sequencing , Immunoglobulins/metabolism , Interferon Regulatory Factors/genetics , Mice , Mice, Knockout , Microscopy, Electron, Transmission , Plasma Cells/metabolism , Positive Regulatory Domain I-Binding Factor 1 , Regulatory Factor X Transcription Factors , Sequence Analysis, DNA , TOR Serine-Threonine Kinases/genetics , TOR Serine-Threonine Kinases/immunology , Transcription Factors/genetics , Unfolded Protein Response/genetics , X-Box Binding Protein 1ABSTRACT
The transcription factor Blimp-1 is necessary for the generation of plasma cells. Here we studied its functions in plasmablast differentiation by identifying regulated Blimp-1 target genes. Blimp-1 promoted the migration and adhesion of plasmablasts. It directly repressed genes encoding several transcription factors and Aicda (which encodes the cytidine deaminase AID) and thus silenced B cell-specific gene expression, antigen presentation and class-switch recombination in plasmablasts. It directly activated genes, which led to increased expression of the plasma cell regulator IRF4 and proteins involved in immunoglobulin secretion. Blimp-1 induced the transcription of immunoglobulin genes by controlling the 3' enhancers of the loci encoding the immunoglobulin heavy chain (Igh) and κ-light chain (Igk) and, furthermore, regulated the post-transcriptional expression switch from the membrane-bound form of the immunoglobulin heavy chain to its secreted form by activating Ell2 (which encodes the transcription-elongation factor ELL2). Notably, Blimp-1 recruited chromatin-remodeling and histone-modifying complexes to regulate its target genes. Hence, many essential functions of plasma cells are under the control of Blimp-1.
Subject(s)
Cell Differentiation/immunology , Immunoglobulin Heavy Chains/immunology , Immunoglobulin kappa-Chains/immunology , Interferon Regulatory Factors/immunology , Plasma Cells/immunology , Transcription Factors/immunology , Animals , B-Lymphocytes/immunology , B-Lymphocytes/metabolism , Cell Adhesion/genetics , Cell Adhesion/immunology , Cell Differentiation/genetics , Cell Migration Assays, Leukocyte , Cell Movement/genetics , Cell Movement/immunology , Chromatin Immunoprecipitation , Electrophoretic Mobility Shift Assay , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Gene Expression Regulation , HEK293 Cells , High-Throughput Nucleotide Sequencing , Humans , Immunoglobulin Heavy Chains/genetics , Immunoglobulin kappa-Chains/genetics , Interferon Regulatory Factors/genetics , Mass Spectrometry , Mice , Plasma Cells/metabolism , Positive Regulatory Domain I-Binding Factor 1 , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Sequence Analysis, RNA , Transcription Factors/geneticsABSTRACT
Regulatory T cells (Treg cells) can express the transcription factors T-bet and GATA-3, but the function of this expression and whether such cells represent stable subsets is still unknown. By using various reporter tools, we found that the expression of T-bet and GATA-3 in Treg cells was dynamically influenced by the cytokine environment. Treg cell-specific deletion of the gene encoding either T-bet (Tbx21) or GATA-3 (Gata3) alone did not result in loss of Treg cell function; however, mice with combined deficiency in both genes in Treg cells developed severe autoimmune-like diseases. Loss of Treg cell function correlated with upregulation of expression of the transcription factor RORγt and reduced expression of the transcription factor Foxp3. Thus, in the steady state, activated Treg cells transiently upregulated either T-bet or GATA-3 to maintain T cell homeostasis.
Subject(s)
GATA3 Transcription Factor/genetics , GATA3 Transcription Factor/metabolism , Gene Expression Regulation , Immune Tolerance/genetics , T-Box Domain Proteins/genetics , T-Box Domain Proteins/metabolism , T-Lymphocytes, Regulatory/immunology , Animals , Autoimmunity , Cells, Cultured , Colitis/immunology , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , MiceABSTRACT
Foxp3(+) regulatory T cells (Treg cells) are required for immunological homeostasis. One notable distinction between conventional T cells (Tconv cells) and Treg cells is differences in the activity of phosphatidylinositol-3-OH kinase (PI(3)K); only Tconv cells downregulate PTEN, the main negative regulator of PI(3)K, upon activation. Here we found that control of PI(3)K in Treg cells was essential for lineage homeostasis and stability. Mice lacking Pten in Treg cells developed an autoimmune-lymphoproliferative disease characterized by excessive T helper type 1 (TH1) responses and B cell activation. Diminished control of PI(3)K activity in Treg cells led to reduced expression of the interleukin-2 (IL-2) receptor α subunit CD25, accumulation of Foxp3(+)CD25(-) cells and, ultimately, loss of expression of the transcription factor Foxp3 in these cells. Collectively, our data demonstrate that control of PI(3)K signaling by PTEN in Treg cells is critical for maintaining their homeostasis, function and stability.
Subject(s)
Homeostasis/immunology , Phosphatidylinositol 3-Kinases/metabolism , T-Lymphocytes, Regulatory/enzymology , T-Lymphocytes, Regulatory/immunology , Animals , Cell Lineage , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Gene Deletion , Mice , PTEN Phosphohydrolase/metabolism , Phosphatidylinositol 3-Kinases/genetics , Signal TransductionABSTRACT
Dengue is a rapidly emerging, mosquito-borne viral infection, with an estimated 400 million infections occurring annually. To gain insight into dengue immunity, we characterized 145 human monoclonal antibodies (mAbs) and identified a previously unknown epitope, the envelope dimer epitope (EDE), that bridges two envelope protein subunits that make up the 90 repeating dimers on the mature virion. The mAbs to EDE were broadly reactive across the dengue serocomplex and fully neutralized virus produced in either insect cells or primary human cells, with 50% neutralization in the low picomolar range. Our results provide a path to a subunit vaccine against dengue virus and have implications for the design and monitoring of future vaccine trials in which the induction of antibody to the EDE should be prioritized.
Subject(s)
Antibodies, Neutralizing/isolation & purification , Dengue Virus/immunology , Dengue/immunology , Viral Envelope Proteins/immunology , Animals , Antibodies, Monoclonal/blood , Antibodies, Monoclonal/isolation & purification , Antibodies, Monoclonal/metabolism , Antibodies, Neutralizing/blood , Biological Assay , Cell Line , Dengue/blood , Enzyme-Linked Immunosorbent Assay , Humans , Immunoblotting , Viral Envelope Proteins/metabolismABSTRACT
How precursor frequencies and antigen affinities impact interclonal B cell competition is a particularly relevant issue for candidate germline-targeting HIV vaccine designs because of the in vivo rarity of naive B cells that recognize broadly neutralizing epitopes. Knowing the frequencies and affinities of HIV-specific VRC01-class naive human B cells, we transferred B cells with germline VRC01 B cell receptors into congenic recipients to elucidate the roles of precursor frequency, antigen affinity, and avidity on B cell responses following immunization. All three factors were interdependently limiting for competitive success of VRC01-class B cells. In physiological high-affinity conditions using a multivalent immunogen, rare VRC01-class B cells successfully competed in germinal centers (GC), underwent extensive somatic hypermutation, and differentiated into memory B cells. The data reveal dominant influences of precursor frequency, affinity, and avidity for interclonal GC competition and indicate that germline-targeting immunogens can overcome these challenges with high-affinity multimeric designs.