ABSTRACT
Biological electron transfer is challenging to directly regulate using environmental conditions. To enable dynamic, protein-level control over energy flow in metabolic systems for synthetic biology and bioelectronics, we created ferredoxin logic gates that utilize transcriptional and post-translational inputs to control energy flow through a synthetic electron transfer pathway that is required for bacterial growth. These logic gates were created by subjecting a thermostable, plant-type ferredoxin to backbone fission and fusing the resulting fragments to a pair of proteins that self-associate, a pair of proteins whose association is stabilized by a small molecule, and to the termini of a ligand-binding domain. We show that the latter domain insertion design strategy yields an allosteric ferredoxin switch that acquires an oxygen-tolerant [2Fe-2S] cluster and can use different chemicals, including a therapeutic drug and an environmental pollutant, to control the production of a reduced metabolite in Escherichia coli and cell lysates.
Subject(s)
Electron Transport/physiology , Metalloproteins/physiology , Amino Acid Sequence , Electron Spin Resonance Spectroscopy/methods , Electron Transport/drug effects , Electrons , Escherichia coli/metabolism , Ferredoxins/physiology , Metalloproteins/genetics , Mutagenesis, Site-Directed/methods , Protein Processing, Post-Translational/physiologyABSTRACT
Iron chronically limits aquatic photosynthesis, especially in marine environments, and the correct perception and maintenance of iron homeostasis in photosynthetic bacteria, including cyanobacteria, is therefore of global significance. Multiple adaptive mechanisms, responsive promoters, and posttranscriptional regulators have been identified, which allow cyanobacteria to respond to changing iron concentrations. However, many factors remain unclear, in particular, how iron status is perceived within the cell. Here we describe a cyanobacterial ferredoxin (Fed2), with a unique C-terminal extension, that acts as a player in iron perception. Fed2 homologs are highly conserved in photosynthetic organisms from cyanobacteria to higher plants, and, although they belong to the plant type ferredoxin family of [2Fe-2S] photosynthetic electron carriers, they are not involved in photosynthetic electron transport. As deletion of fed2 appears lethal, we developed a C-terminal truncation system to attenuate protein function. Disturbed Fed2 function resulted in decreased chlorophyll accumulation, and this was exaggerated in iron-depleted medium, where different truncations led to either exaggerated or weaker responses to low iron. Despite this, iron concentrations remained the same, or were elevated in all truncation mutants. Further analysis established that, when Fed2 function was perturbed, the classical iron limitation marker IsiA failed to accumulate at transcript and protein levels. By contrast, abundance of IsiB, which shares an operon with isiA, was unaffected by loss of Fed2 function, pinpointing the site of Fed2 action in iron perception to the level of posttranscriptional regulation.
Subject(s)
Ferredoxins/physiology , Iron/metabolism , Photosynthesis/physiology , Synechocystis/physiology , Adaptation, Physiological , Chlorophyll/metabolism , Ferredoxins/chemistry , Ferredoxins/metabolism , Homeostasis/genetics , Synechocystis/genetics , Synechocystis/metabolismABSTRACT
Defects in iron-sulphur [Fe-S] cluster biogenesis are increasingly recognized as causing neurological disease. Mutations in a number of genes that encode proteins involved in mitochondrial [Fe-S] protein assembly lead to complex neurological phenotypes. One class of proteins essential in the early cluster assembly are ferredoxins. FDX2 is ubiquitously expressed and is essential in the de novo formation of [2Fe-2S] clusters in humans. We describe and genetically define a novel complex neurological syndrome identified in two Brazilian families, with a novel homozygous mutation in FDX2. Patients were clinically evaluated, underwent MRI, nerve conduction studies, EMG and muscle biopsy. To define the genetic aetiology, a combination of homozygosity mapping and whole exome sequencing was performed. We identified six patients from two apparently unrelated families with autosomal recessive inheritance of a complex neurological phenotype involving optic atrophy and nystagmus developing by age 3, followed by myopathy and recurrent episodes of cramps, myalgia and muscle weakness in the first or second decade of life. Sensory-motor axonal neuropathy led to progressive distal weakness. MRI disclosed a reversible or partially reversible leukoencephalopathy. Muscle biopsy demonstrated an unusual pattern of regional succinate dehydrogenase and cytochrome c oxidase deficiency with iron accumulation. The phenotype was mapped in both families to the same homozygous missense mutation in FDX2 (c.431C > T, p.P144L). The deleterious effect of the mutation was validated by real-time reverse transcription polymerase chain reaction and western blot analysis, which demonstrated normal expression of FDX2 mRNA but severely reduced expression of FDX2 protein in muscle tissue. This study describes a novel complex neurological phenotype with unusual MRI and muscle biopsy features, conclusively mapped to a mutation in FDX2, which encodes a ubiquitously expressed mitochondrial ferredoxin essential for early [Fe-S] cluster biogenesis.
Subject(s)
Ferredoxins/genetics , Ferredoxins/physiology , Adolescent , Adult , Brazil , Child , Electron Transport Complex IV/metabolism , Female , Homozygote , Humans , Iron/metabolism , Iron-Sulfur Proteins/genetics , Iron-Sulfur Proteins/physiology , Leukoencephalopathies/metabolism , Male , Mitochondria/metabolism , Mitochondrial Proteins/genetics , Muscular Diseases/genetics , Myalgia/genetics , Optic Atrophy/genetics , Pedigree , Phenotype , Succinate Dehydrogenase/metabolism , Syndrome , Exome SequencingABSTRACT
Ferredoxin (Fd) is a small [2Fe-2S] cluster-containing protein found in all organisms performing oxygenic photosynthesis. Fd is the first soluble acceptor of electrons on the stromal side of the chloroplast electron transport chain, and as such is pivotal to determining the distribution of these electrons to different metabolic reactions. In chloroplasts, the principle sink for electrons is in the production of NADPH, which is mostly consumed during the assimilation of CO2 . In addition to this primary function in photosynthesis, Fds are also involved in a number of other essential metabolic reactions, including biosynthesis of chlorophyll, phytochrome and fatty acids, several steps in the assimilation of sulphur and nitrogen, as well as redox signalling and maintenance of redox balance via the thioredoxin system and Halliwell-Asada cycle. This makes Fds crucial determinants of the electron transfer between the thylakoid membrane and a variety of soluble enzymes dependent on these electrons. In this article, we will first describe the current knowledge on the structure and function of the various Fd isoforms present in chloroplasts of higher plants and then discuss the processes involved in oxidation of Fd, introducing the corresponding enzymes and discussing what is known about their relative interaction with Fd.
Subject(s)
Chloroplasts/metabolism , Ferredoxins/metabolism , Photosynthesis , Plants/metabolism , Chloroplasts/physiology , Electron Transport/physiology , Ferredoxins/physiology , Photosynthesis/physiologyABSTRACT
Mammalian adrenodoxin (Adx) has been known for many years as an essential electron mediator in mitochondrial cytochrome P450 systems. Because of its ability to support several cytochrome P450 enzymes, it is involved not only in adrenal steroid hormone biosynthesis but also in vitamin D and bile acid metabolism. Recently, Adx is increasingly gaining attention because of its potential for pharmaceutical industry and biotechnology. With human cytochromes P450 becoming important drug targets, suitable Adx-based screening systems have to be developed to test putative new drugs. Moreover, in artificial systems, Adx has been shown to functionally interact with diverse bacterial cytochromes P450 catalyzing a variety of chemically interesting reactions. Putative biotechnological applications of such Adx-containing reconstituted systems are discussed.
Subject(s)
Adrenodoxin/physiology , Ferredoxins/physiology , Adrenodoxin/biosynthesis , Adrenodoxin/chemistry , Animals , Bacterial Proteins/chemistry , Bacterial Proteins/physiology , Coenzymes/biosynthesis , Cytochrome P-450 Enzyme System/chemistry , Cytochrome P-450 Enzyme System/physiology , Drug Evaluation, Preclinical , Ferredoxins/biosynthesis , Ferredoxins/chemistry , Humans , Mitochondria/enzymology , Oxidation-Reduction , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistryABSTRACT
High-salinity stress is one of the major limiting factors on crop productivity. Physiological strategies against high-salinity stress include generation of reactive oxygen species (ROS), induction of stress-related genes expression, accumulation of abscisic acid (ABA) and up-regulation of antiporters. ROS are metabolism by-products and involved in signal transduction pathway. Constitutive expression of plant ferrodoxin-like protein (PFLP) gene enhances pathogen-resistance activities and root-hair growth through promoting ROS generation. However, the function of PFLP in abiotic stress responses is unclear. In this study, PFLP-1 and PFLP-2-transgenic rice plants were generated to elucidate the role of PFLP under salinity stress. PFLP overexpression significantly increased salt tolerance in PFLP-transgenic rice plants compared with non-transgenic plants (Oryza sativa japonica cv. Tainung 67, designated as TNG67). In high-salinity conditions, PFLP-transgenic plants exhibited earlier ROS production, higher antioxidant enzyme activities, higher ABA accumulation, up-regulated expression of stress-related genes (OsRBOHa, Cu/Zn SOD, OsAPX, OsNCED2, OsSOS1, OsCIPK24, OsCBL4, and OsNHX2), and leaf sodium ion content was lower compared with TNG67 plant. In addition, transgenic lines maintained electron transport rates and contained lower malondialdhyde (MDA) content than TNG67 plant did under salt-stress conditions. Overall results indicated salinity tolerance was improved by PFLP overexpression in transgenic rice plant. The PFLP gene is a potential candidate for improving salinity tolerance for valuable agricultural crops.
Subject(s)
Ferredoxins/physiology , Oryza , Salt Tolerance , Gene Expression Regulation, Plant , Oryza/physiology , Plant Proteins/physiology , Plants, Genetically Modified/physiology , SalinityABSTRACT
Ferredoxin:thioredoxin reductase catalyzes the reduction of thioredoxins in plant chloroplasts using the [Fe2S2] ferredoxin as a one-electron donor and as such plays a central role in light regulation of oxygenic photosynthesis. The active-site comprises a [Fe4S4] cluster next to a redox-active disulfide that is cleaved in sequential one-electron steps and the combination of spectroscopic and crystallographic studies have revealed a catalytic mechanism involving novel site specific cluster chemistry in the oxidized, one-electron- and two-electron-reduced redox states. Histidine-86 has emerged as a potential proton donor/acceptor in the catalytic mechanism based on redox-related changes in the positioning of the imidazole ring during redox cycling and greatly decreased activity for the H86Y variant. Here we report on spectroscopic and redox characterization of the [Fe4S4] center in Synechocystis sp. PCC 6803 H86Y ferredoxin:thoredoxin reductase in the accessible redox states of both the as purified and N-ethylmaleimide-modified forms, using the combination of UV-visible absorption and variable-temperature magnetic circular dichroism, EPR, resonance Raman and Mössbauer spectroscopies. The results demonstrate that His86 is required for formation of the partially valence-localized [Fe4S4]2+ cluster that is the hallmark of two-electron-reduced intermediate. Taken together with the available structural data, the spectroscopic results indicate a functional role for His86 in protonation/deprotonation of the cluster-interacting thiol and anchoring the cluster interacting thiol in close proximity to the cluster in the two-electron-reduced intermediate.
Subject(s)
Bacterial Proteins/chemistry , Ferredoxins/chemistry , Histidine/chemistry , Thioredoxin-Disulfide Reductase/chemistry , Bacterial Proteins/metabolism , Bacterial Proteins/physiology , Catalysis , Electrons , Ferredoxins/physiology , Histidine/genetics , Iron-Sulfur Proteins/chemistry , Oxidation-Reduction , Protons , Sulfhydryl Compounds/chemistry , Synechocystis/enzymology , Synechocystis/metabolism , Thioredoxin-Disulfide Reductase/physiologyABSTRACT
Photosynthetic complex I enables cyclic electron flow around photosystem I, a regulatory mechanism for photosynthetic energy conversion. We report a 3.3-angstrom-resolution cryo-electron microscopy structure of photosynthetic complex I from the cyanobacterium Thermosynechococcus elongatus. The model reveals structural adaptations that facilitate binding and electron transfer from the photosynthetic electron carrier ferredoxin. By mimicking cyclic electron flow with isolated components in vitro, we demonstrate that ferredoxin directly mediates electron transfer between photosystem I and complex I, instead of using intermediates such as NADPH (the reduced form of nicotinamide adenine dinucleotide phosphate). A large rate constant for association of ferredoxin to complex I indicates efficient recognition, with the protein subunit NdhS being the key component in this process.
Subject(s)
Cyanobacteria/physiology , Electron Transport Complex I/physiology , Ferredoxins/physiology , Photosynthesis , Photosystem I Protein Complex/physiology , Cryoelectron Microscopy , Electron Transport , Models, Molecular , Protein Structure, QuaternaryABSTRACT
Ferredoxins are the major distributors for electrons to the various acceptor systems in plastids. In green tissues, ferredoxins are reduced by photosynthetic electron flow in the light, while in heterotrophic tissues, nicotinamide adenine dinucleotide (reduced) (NADPH) generated in the oxidative pentose-phosphate pathway (OPP) is the reductant. We have used a Ds-T-DNA insertion line of Arabidopsis thaliana for the gene encoding the major leaf ferredoxin (Fd2, At1g60950) to create a situation of high electron pressure in the thylakoids. Although these plants (Fd2-KO) possess only the minor fraction of leaf Fd1 (At1g10960), they grow photoautotrophically on soil, but with a lower growth rate and less chlorophyll. The more oxidized conditions in the stroma due to the formation of reactive oxygen species are causing a re-adjustment of the redox state in these plants that helps them to survive even under high light. Redox homeostasis is achieved by regulation at both, the post-translational and the transcriptional level. Over-reduction of the electron transport chain leads to increased transcription of the malate-valve enzyme NADP-malate dehydrogenase (MDH), and the oxidized stroma leads to an increased transcription of the OPP enzyme glucose-6-P dehydrogenase. In isolated spinach chloroplasts, oxidized conditions give rise to a decreased activation state of NADP-MDH and an activation of glucose-6-P dehydrogenase even in the light. In Fd2-KO plants, NADPH-requiring antioxidant systems are upregulated. These adjustments must be caused by plastid signals, and they prevent oxidative damage under rather severe conditions.
Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Ferredoxins/genetics , Mutation , Plant Leaves/genetics , Arabidopsis/metabolism , Arabidopsis/physiology , Arabidopsis Proteins/metabolism , Arabidopsis Proteins/physiology , Blotting, Western , Chlorophyll/metabolism , Ferredoxins/metabolism , Ferredoxins/physiology , Gene Expression Regulation, Plant , Oxidation-Reduction , Photosynthesis/genetics , Photosynthesis/physiology , Plant Leaves/metabolism , Plant Leaves/physiology , Plants, Genetically Modified , Reactive Oxygen Species/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction/genetics , Signal Transduction/physiologyABSTRACT
Desulfoferrodoxin (cac2450) of Clostridium acetobutylicum was purified after overexpression in E. coli. In an in vitro assay the enzyme exhibited superoxide reductase activity with rubredoxin (cac2778) of C. acetobutylicum as the proximal electron donor. Rubredoxin was reduced by ferredoxin:NADP(+) reductase from spinach and NADPH. The superoxide anions, generated from dissolved oxygen using Xanthine and Xanthine oxidase, were reduced to hydrogen peroxide. Thus, we assume that desulfoferrodoxin is the key factor in the superoxide reductase dependent part of an alternative pathway for detoxification of reactive oxygen species in this obligate anaerobic bacterium.
Subject(s)
Bacterial Proteins/physiology , Clostridium acetobutylicum/enzymology , Ferredoxins/physiology , Oxidoreductases/physiology , Cytochromes c/metabolism , Ferredoxins/isolation & purification , Hydrogen Peroxide/metabolism , Oxidation-Reduction , Reactive Oxygen Species/metabolism , Rubredoxins/isolation & purification , Superoxide Dismutase/metabolism , Superoxides/metabolismABSTRACT
Iron is an essential nutrient for nearly all organisms but presents problems of toxicity, poor solubility and low availability. These problems are alleviated through the use of iron-storage proteins. Bacteria possess two types of iron-storage protein, the haem-containing bacterioferritins and the haem-free ferritins. These proteins are widespread in bacteria, with at least 39 examples known so far in eubacteria and archaebacteria. The bacterioferritins and ferritins are distantly related but retain similar structural and functional properties. Both are composed of 24 identical or similar subunits (approximately 19 kDa) that form a roughly spherical protein (approximately 450 kDa, approximately 120 A diameter) containing a large hollow centre (approximately 80 A diameter). The hollow centre acts as an iron-storage cavity with the capacity to accommodate at least 2000 iron atoms in the form of a ferric-hydroxyphosphate core. Each subunit contains a four-helix bundle which carries the active site or ferroxidase centre of the protein. The ferroxidase centres endow ferrous-iron-oxidizing activity and are able to form a di-iron species that is an intermediate in the iron uptake, oxidation and core formation process. Bacterioferritins contain up to 12 protoporphyrin IX haem groups located at the two-fold interfaces between pairs of two-fold related subunits. The role of the haem is unknown, although it may be involved in mediating iron-core reduction and iron release. Some bacterioferritins are composed of two subunit types, one conferring haem-binding ability (alpha) and the other (beta) bestowing ferroxidase activity. Bacterioferritin genes are often adjacent to genes encoding a small [2Fe-2S]-ferredoxin (bacterioferritin-associated ferredoxin or Bfd). Bfd may directly interact with bacterioferritin and could be involved in releasing iron from (or delivering iron to) bacterioferritin or other iron complexes. Some bacteria contain two bacterioferritin subunits, or two ferritin subunits, that in most cases co-assemble. Others possess both a bacterioferritin and a ferritin, while some appear to lack any type of iron-storage protein. The reason for these differences is not understood. Studies on ferritin mutants have shown that ferritin enhances growth during iron starvation and is also involved in iron accumulation in the stationary phase of growth. The ferritin of Campylobacter jejuni is involved in redox stress resistance, although this does not appear to be the case for Escherichia coli ferritin (FtnA). No phenotype has been determined for E. coli bacterioferritin mutants and the precise role of bacterioferritin in E. coli remains uncertain.
Subject(s)
Cytochrome b Group/physiology , Escherichia coli/physiology , Ferritins/physiology , Bacterial Proteins/metabolism , Bacterial Proteins/physiology , Campylobacter jejuni/metabolism , Campylobacter jejuni/physiology , Cytochrome b Group/metabolism , Escherichia coli/metabolism , Ferredoxins/metabolism , Ferredoxins/physiology , Ferritins/metabolism , Gene Expression Regulation, Bacterial , Heme/metabolism , Hemerythrin , Rubredoxins , Structure-Activity Relationship , Superoxide Dismutase/metabolism , Superoxide Dismutase/physiologyABSTRACT
In our efforts to determine the components participating in the electron transport to nitrogenase in Rhodospirillum rubrum, we have identified a gene encoding a new ferredoxin. We have generated mutants in both the new ferredoxin and ferredoxin I and demonstrate that the new ferredoxin, FdN and not the previously identified FdI is the main donor of electrons to nitrogenase.
Subject(s)
Electron Transport , Ferredoxins/genetics , Ferredoxins/physiology , Nitrogenase/metabolism , Rhodospirillum rubrum/enzymology , Bacterial Proteins/genetics , Bacterial Proteins/physiology , Mutagenesis, Insertional , Mutation , Rhodospirillum rubrum/geneticsABSTRACT
Pyrococcus furiosus ferredoxin is subject to a monomer/dimer equilibrium as a function of ionic strength. At physiological ionic strength, approximately 0.35 M NaCl, the protein is very predominantly homodimer. The monomeric form exhibits impaired electron transfer on glassy carbon; it also has a decreased S=3/2 over S=1/2 ratio as shown by electron paramagnetic resonance spectroscopy. Even following sterilization at 121 degrees C the dimer is stable in denaturing gel electrophoresis.
Subject(s)
Ferredoxins/chemistry , Ferredoxins/physiology , Pyrococcus furiosus , Chromatography, Gel , Dimerization , Electron Spin Resonance Spectroscopy , Electron Transport , Electrophoresis, Polyacrylamide Gel , Ferredoxins/isolation & purification , Osmolar ConcentrationABSTRACT
Specific metabolic roles of P450-dependent monooxygenase systems are determined by enzymatic properties and substrate specificity of P450s, the terminal enzymes of the electron transfer chain. On the other hand, molecular diversity has also been reported for NADPH-cytochrome P450 reductase, cytochrome b5, and cytochrome b5 reductase in plants. Several lines of evidence indicate that the electron transfer components for plant P450 reactions have specific physiological roles. In this review, we describe the current status of knowledge of the biochemistry, molecular biology, gene regulation, and molecular diversity of plant P450-related electron transfer components and summarize possible individual physiological roles of the diversified P450 electron transfer systems in plants.
Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Electron Transport Chain Complex Proteins/physiology , Plant Proteins/physiology , Plants/metabolism , Amino Acid Sequence , Chloroplasts/metabolism , Cytochrome c Group/chemistry , Cytochrome c Group/physiology , Electron Transport Chain Complex Proteins/chemistry , Electron Transport Chain Complex Proteins/genetics , Ferredoxin-NADP Reductase/physiology , Ferredoxins/physiology , Gene Expression Regulation, Plant , Mitochondrial Proteins/physiology , Molecular Sequence Data , NADPH-Ferrihemoprotein Reductase/genetics , NADPH-Ferrihemoprotein Reductase/physiology , Plant Proteins/chemistry , Plant Proteins/genetics , Plants/enzymology , Plants/geneticsABSTRACT
The inhibitory effect of a range of nitroimidazole-derivatives on H2 production by metronidazole resistant (CDC-85) and susceptible (C1-NIH) Trichomonas vaginalis strains was investigated. The 2-, 4-, and 5-nitro-derivatives used had one-electron reduction potentials within the range -250 to -525 mV. Nitroimidazole concentrations giving 50% inhibition of H2 production (kiH2) for compounds with one-electron reduction potentials in the range -250 to -425 mV were found to be similar for both strains tested. Compounds with one-electron reduction potentials below -425 mV give 10-fold higher KiH2 values for the metronidazole resistant isolate. Both strains showed increased KiH2 for compounds with potentials lower than -500 mV. The addition of 2.1 kPa (0.02 atm) O2 to the gas phase resulted in increasing the kiH2 values for all the compounds tested, but had the greater effect on results obtained with the resistant isolate using nitroimidazoles in the range -425 to -490 mV. The results enable the proposal that the resistant isolate CDC-85 has a ferredoxin with altered redox properties or reduced intracellular levels.
Subject(s)
Ferredoxins/physiology , Nitroimidazoles/pharmacology , Trichomonas vaginalis/metabolism , Animals , Cell Movement , Drug Resistance, Microbial , Hydrogen/metabolism , Kinetics , Mass Spectrometry , Oxidation-Reduction , Oxygen/pharmacology , Trichomonas vaginalis/cytology , Trichomonas vaginalis/drug effectsABSTRACT
The NAD(P)H-dependent nitrate reductase system in Clostridium perfringens was reconstituted with rubredoxin (Rd), nitrate reductase (NaR), and an unadsorbed fraction, on a DEAE-cellulose column, of the extract (designated as fraction A), under nitrogen gas. Ferredoxin in place of Rd was not effective as an electron carrier in this reconstituted system. NAD(P)H-dependent nitrate reducing activity was also obtained by replacing fraction A with ferredoxin-NADP+ reductase from spinach. We propose the following scheme for the electron transfer in this NAD(P)H dependent nitrate reduction system. NAD(P)H----NAD(P)H-Rd reductase----Rd----NaR----NO3-.
Subject(s)
Clostridium perfringens/metabolism , Ferredoxins/physiology , NADP/metabolism , Nitrates/metabolism , Rubredoxins/physiology , Electron Transport , Oxidation-ReductionABSTRACT
The reporter gene xylE (encoding catechol 2,3-dioxygenase) has been modified for a more rational use in Streptomyces. Two reporter fragments, one containing xylE, and the other containing also the upstream gene xylT (which encodes a soluble ferredoxin), have been constructed to allow precise fusion of regulatory regions to the reporter genes. Identical fusions of these xylE and xylTE reporter fragments to the Streptomyces dagA and tipA promoters, in low and high copy number plasmids, show that the levels of xylE mRNA and catechol 2,3-dioxygenase activities are significantly higher when xylT is present.
Subject(s)
Bacterial Proteins , Dioxygenases , Ferredoxins/genetics , Genes, Reporter , Oxygenases/genetics , Streptomyces griseus/genetics , Base Sequence , Catechol 2,3-Dioxygenase , DNA, Bacterial/chemistry , Ferredoxins/isolation & purification , Ferredoxins/physiology , Gene Expression Regulation, Bacterial , Genetic Vectors , Molecular Sequence Data , Oxygenases/isolation & purification , Promoter Regions, Genetic , Restriction Mapping , Streptomyces/genetics , Streptomyces/metabolism , Streptomyces griseus/metabolismABSTRACT
Comparison of the N-terminus of the heat shock protein Hsp21 of Clostridium acetobutylicum with proteins predicted to be encoded by the genome of this bacterium revealed that this stress protein is encoded by two almost identical open reading frames CAC3597 and CAC3598. These genes encode a rubrerythrin-like protein with the rubredoxin-like FeS4 domain at the N-terminus and the ferritin-like diiron domain (rubrerythrin domain) at the C-terminus. Thus, the order of the two putative functional domains is reversed compared to "normal" rubrerythrins. This protein is proposed to be involved in the oxidative stress response of strict anaerobic bacteria. Northern blot analysis indicated that hsp21 is induced by heat and oxidative stress (air, H2O2). Hsp21 of C. acetobutylicum can be considered as a "reverse" rubrerythrin and a role of this stress protein, which is conserved among clostridia and other strict anaerobic bacteria, in the heat and oxidative stress response is proposed.
Subject(s)
Bacterial Proteins/genetics , Clostridium/genetics , Ferredoxins/genetics , Genes, Bacterial , Heat-Shock Proteins/genetics , Heat-Shock Proteins/physiology , Oxidative Stress , Adaptation, Physiological , Air , Bacterial Proteins/physiology , Blotting, Western , Clostridium/metabolism , Ferredoxins/physiology , Ferritins/genetics , Gene Expression Regulation, Bacterial , Genes, Duplicate , Hemerythrin , Hot Temperature , Hydrogen Peroxide/toxicity , Oxygen/toxicity , Protein Structure, Tertiary , Proteome/analysis , Rubredoxins/genetics , Sequence HomologyABSTRACT
Homologues of the selenoprotein glutathione peroxidase (GPx) have been previously identified in poxviruses and in RNA viruses including HIV-1 and hepatitis C virus (HCV). Sequence analysis of the NS4 region of Japanese encephalitis virus (JEV) suggests it may encode a structurally related but functionally distinct selenoprotein gene, more closely related to the iron-binding protein ferredoxin than to GPx, with three highly conserved UGA codons that align with essential Cys residues of ferredoxin. Comparison of the probe JEV sequence to an aligned family of ferredoxin sequences gave an overall 30.3% identity and 45.8% similarity, and was statistically significant at 4.9 S.D. (P < 10(-6)) above the average score computed for randomly shuffled sequences. A 3-dimensional model of the hypothetical JEV protein (JEV model) was constructed by homology modeling using SYBYL, based upon a high resolution X-ray structure of ferredoxin (PDB code: 1awd). The JEV model and the model from 1awd were subsequently subjected to molecular dynamics simulations in aqueous medium using AMBER 6. The solution structure of the JEV model indicates that it could fold into a tertiary structure globally similar to ferredoxin 1awd, with RMSD between the averaged structures of 1.8 A for the aligned regions. The modeling and MD simulations data also indicate that this structure for the JEV protein is energetically favorable, and that it could be quite stable at room temperature. This protein might play a role in JEV infection and replication via TNF and other cellular stimuli mediated via redox mechanisms.
Subject(s)
Encephalitis Virus, Japanese/genetics , Ferredoxins/chemistry , Models, Structural , Proteins/chemistry , Viral Nonstructural Proteins/chemistry , Amino Acid Sequence , Computational Biology , Computer Simulation , Cysteine/chemistry , Encephalitis Virus, Japanese/physiology , Ferredoxins/genetics , Ferredoxins/physiology , Molecular Sequence Data , Protein Structure, Tertiary , Proteins/genetics , Proteins/physiology , Selenoproteins , Sequence Alignment , Viral Nonstructural Proteins/genetics , Viral Nonstructural Proteins/physiologyABSTRACT
Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a tobacco cDNA library. Screening identified a new Tsip1-interacting protein, Nicotiana tabacum Tsip1-interacting ferredoxin 1 (NtTfd1), and binding specificity was confirmed both in vitro and in vivo. The four repeats of a cysteine-rich motif (CXXCXGXG) of Tsip1 proved important for binding to NtTfd1. Virus-induced gene silencing of NtTfd1, Tsip1, and NtTfd1/Tsip1 rendered plants more susceptible to salinity stress compared with TRV2 control plants. NtTfd1- and Tsip1-silenced tobacco plants were more susceptible to infection by Cucumber mosaic virus compared with control plants. These results suggest that NtTfd1 might be involved in the regulation of biotic and abiotic stresses in chloroplasts by interaction with Tsip1.