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1.
Cell ; 187(16): 4272-4288.e20, 2024 Aug 08.
Article in English | MEDLINE | ID: mdl-39013469

ABSTRACT

Vesicle trafficking is a fundamental process that allows for the sorting and transport of specific proteins (i.e., "cargoes") to different compartments of eukaryotic cells. Cargo recognition primarily occurs through coats and the associated proteins at the donor membrane. However, it remains unclear whether cargoes can also be selected at other stages of vesicle trafficking to further enhance the fidelity of the process. The WDR11-FAM91A1 complex functions downstream of the clathrin-associated AP-1 complex to facilitate protein transport from endosomes to the TGN. Here, we report the cryo-EM structure of human WDR11-FAM91A1 complex. WDR11 directly and specifically recognizes a subset of acidic clusters, which we term super acidic clusters (SACs). WDR11 complex assembly and its binding to SAC-containing proteins are indispensable for the trafficking of SAC-containing proteins and proper neuronal development in zebrafish. Our studies thus uncover that cargo proteins could be recognized in a sequence-specific manner downstream of a protein coat.


Subject(s)
Cryoelectron Microscopy , Protein Transport , Zebrafish , Humans , Animals , Endosomes/metabolism , HEK293 Cells , HeLa Cells , Zebrafish Proteins/metabolism , Zebrafish Proteins/chemistry , Protein Binding
2.
Cell ; 184(26): 6313-6325.e18, 2021 12 22.
Article in English | MEDLINE | ID: mdl-34942099

ABSTRACT

How tissues acquire complex shapes is a fundamental question in biology and regenerative medicine. Zebrafish semicircular canals form from invaginations in the otic epithelium (buds) that extend and fuse to form the hubs of each canal. We find that conventional actomyosin-driven behaviors are not required. Instead, local secretion of hyaluronan, made by the enzymes uridine 5'-diphosphate dehydrogenase (ugdh) and hyaluronan synthase 3 (has3), drives canal morphogenesis. Charged hyaluronate polymers osmotically swell with water and generate isotropic extracellular pressure to deform the overlying epithelium into buds. The mechanical anisotropy needed to shape buds into tubes is conferred by a polarized distribution of actomyosin and E-cadherin-rich membrane tethers, which we term cytocinches. Most work on tissue morphogenesis ascribes actomyosin contractility as the driving force, while the extracellular matrix shapes tissues through differential stiffness. Our work inverts this expectation. Hyaluronate pressure shaped by anisotropic tissue stiffness may be a widespread mechanism for powering morphological change in organogenesis and tissue engineering.


Subject(s)
Extracellular Space/chemistry , Hyaluronic Acid/pharmacology , Morphogenesis , Organ Specificity , Pressure , Semicircular Canals/cytology , Semicircular Canals/embryology , Actomyosin/metabolism , Animals , Anisotropy , Behavior, Animal , Extracellular Matrix/metabolism , Hyaluronic Acid/biosynthesis , Models, Biological , Morphogenesis/drug effects , Organ Specificity/drug effects , Osmotic Pressure , Semicircular Canals/diagnostic imaging , Stereotyped Behavior , Zebrafish/embryology , Zebrafish Proteins/metabolism
3.
Cell ; 184(23): 5791-5806.e19, 2021 11 11.
Article in English | MEDLINE | ID: mdl-34715025

ABSTRACT

Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine of cilia, the axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model of the 48-nm repeat of a mammalian DMT, derived from a cryoelectron microscopy (cryo-EM) map of the complex isolated from bovine respiratory cilia. The structure uncovers principles of doublet microtubule organization and features specific to vertebrate cilia, including previously unknown MIPs, a luminal bundle of tektin filaments, and a pentameric dynein-docking complex. We identify a mechanism for bridging 48- to 24-nm periodicity across the microtubule wall and show that loss of the proteins involved causes defective ciliary motility and laterality abnormalities in zebrafish and mice. Our structure identifies candidate genes for diagnosis of ciliopathies and provides a framework to understand their functions in driving ciliary motility.


Subject(s)
Cilia/ultrastructure , Cryoelectron Microscopy , Mammals/metabolism , Proteins/metabolism , Proteins/ultrastructure , Amino Acid Sequence , Animals , Cattle , Cilia/metabolism , Dyneins/metabolism , Embryo, Mammalian/metabolism , Female , Male , Mice, Inbred C57BL , Microtubule Proteins/chemistry , Microtubules/metabolism , Microtubules/ultrastructure , Models, Molecular , Mutation/genetics , Trachea/anatomy & histology , Zebrafish , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
4.
Cell ; 184(4): 899-911.e13, 2021 02 18.
Article in English | MEDLINE | ID: mdl-33545089

ABSTRACT

Changes in appendage structure underlie key transitions in vertebrate evolution. Addition of skeletal elements along the proximal-distal axis facilitated critical transformations, including the fin-to-limb transition that permitted generation of diverse modes of locomotion. Here, we identify zebrafish mutants that form supernumerary long bones in their pectoral fins. These new bones integrate into musculature, form joints, and articulate with neighboring elements. This phenotype is caused by activating mutations in previously unrecognized regulators of appendage patterning, vav2 and waslb, that function in a common pathway. This pathway is required for appendage development across vertebrates, and loss of Wasl in mice causes defects similar to those seen in murine Hox mutants. Concordantly, formation of supernumerary bones requires Hox11 function, and mutations in the vav2/wasl pathway drive enhanced expression of hoxa11b, indicating developmental homology with the forearm. Our findings reveal a latent, limb-like pattern ability in fins that is activated by simple genetic perturbation.


Subject(s)
Bone and Bones/embryology , Extremities/embryology , Zebrafish/embryology , Actins/metabolism , Animal Fins/embryology , Animals , Base Sequence , Body Patterning , CRISPR-Cas Systems/genetics , Cell Lineage , Epistasis, Genetic , Gene Expression Regulation, Developmental , Gene Knockout Techniques , Genes, Reporter , HeLa Cells , Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism , Humans , Mice , Mutation/genetics , Phenotype , Phylogeny , Signal Transduction/genetics , Zebrafish/genetics , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
5.
Cell ; 177(4): 970-985.e20, 2019 05 02.
Article in English | MEDLINE | ID: mdl-31031000

ABSTRACT

Prolonged behavioral challenges can cause animals to switch from active to passive coping strategies to manage effort-expenditure during stress; such normally adaptive behavioral state transitions can become maladaptive in psychiatric disorders such as depression. The underlying neuronal dynamics and brainwide interactions important for passive coping have remained unclear. Here, we develop a paradigm to study these behavioral state transitions at cellular-resolution across the entire vertebrate brain. Using brainwide imaging in zebrafish, we observed that the transition to passive coping is manifested by progressive activation of neurons in the ventral (lateral) habenula. Activation of these ventral-habenula neurons suppressed downstream neurons in the serotonergic raphe nucleus and caused behavioral passivity, whereas inhibition of these neurons prevented passivity. Data-driven recurrent neural network modeling pointed to altered intra-habenula interactions as a contributory mechanism. These results demonstrate ongoing encoding of experience features in the habenula, which guides recruitment of downstream networks and imposes a passive coping behavioral strategy.


Subject(s)
Adaptation, Psychological/physiology , Habenula/physiology , Animals , Behavior, Animal/physiology , Brain/metabolism , Habenula/metabolism , Larva , Neural Pathways/metabolism , Neurons/metabolism , Raphe Nuclei/metabolism , Serotonergic Neurons/metabolism , Serotonin , Stress, Physiological/physiology , Zebrafish/metabolism , Zebrafish Proteins/metabolism
6.
Cell ; 177(6): 1463-1479.e18, 2019 05 30.
Article in English | MEDLINE | ID: mdl-31080065

ABSTRACT

Segregation of maternal determinants within the oocyte constitutes the first step in embryo patterning. In zebrafish oocytes, extensive ooplasmic streaming leads to the segregation of ooplasm from yolk granules along the animal-vegetal axis of the oocyte. Here, we show that this process does not rely on cortical actin reorganization, as previously thought, but instead on a cell-cycle-dependent bulk actin polymerization wave traveling from the animal to the vegetal pole of the oocyte. This wave functions in segregation by both pulling ooplasm animally and pushing yolk granules vegetally. Using biophysical experimentation and theory, we show that ooplasm pulling is mediated by bulk actin network flows exerting friction forces on the ooplasm, while yolk granule pushing is achieved by a mechanism closely resembling actin comet formation on yolk granules. Our study defines a novel role of cell-cycle-controlled bulk actin polymerization waves in oocyte polarization via ooplasmic segregation.


Subject(s)
Actins/metabolism , Cell Cycle/physiology , Oocytes/metabolism , Actins/physiology , Animals , Cell Polarity/physiology , Cytoplasm/metabolism , Egg Yolk/physiology , Polymerization , Zebrafish/embryology , Zebrafish/metabolism , Zebrafish Proteins/metabolism , Zygote
7.
Cell ; 176(6): 1379-1392.e14, 2019 03 07.
Article in English | MEDLINE | ID: mdl-30773315

ABSTRACT

Cell fate specification by lateral inhibition typically involves contact signaling through the Delta-Notch signaling pathway. However, whether this is the only signaling mode mediating lateral inhibition remains unclear. Here we show that in zebrafish oogenesis, a group of cells within the granulosa cell layer at the oocyte animal pole acquire elevated levels of the transcriptional coactivator TAZ in their nuclei. One of these cells, the future micropyle precursor cell (MPC), accumulates increasingly high levels of nuclear TAZ and grows faster than its surrounding cells, mechanically compressing those cells, which ultimately lose TAZ from their nuclei. Strikingly, relieving neighbor-cell compression by MPC ablation or aspiration restores nuclear TAZ accumulation in neighboring cells, eventually leading to MPC re-specification from these cells. Conversely, MPC specification is defective in taz-/- follicles. These findings uncover a novel mode of lateral inhibition in cell fate specification based on mechanical signals controlling TAZ activity.


Subject(s)
Intracellular Signaling Peptides and Proteins/metabolism , Oogenesis/physiology , Zebrafish Proteins/metabolism , Adaptor Proteins, Signal Transducing/metabolism , Animals , Cell Communication/physiology , Cell Differentiation/physiology , Cell Lineage , Cell Nucleus/metabolism , Female , Granulosa Cells/metabolism , Intracellular Signaling Peptides and Proteins/antagonists & inhibitors , Oocytes/metabolism , Oocytes/physiology , Protein Serine-Threonine Kinases/metabolism , Signal Transduction , Transcription Factors/metabolism , Transcriptional Activation/physiology , Transcriptional Coactivator with PDZ-Binding Motif Proteins , Zebrafish/metabolism , Zebrafish Proteins/antagonists & inhibitors
8.
Cell ; 172(5): 993-1006.e13, 2018 02 22.
Article in English | MEDLINE | ID: mdl-29456083

ABSTRACT

The fate and function of epigenetic marks during the germline-to-embryo transition is a key issue in developmental biology, with relevance to stem cell programming and transgenerational inheritance. In zebrafish, DNA methylation patterns are programmed in transcriptionally quiescent cleavage embryos; paternally inherited patterns are maintained, whereas maternal patterns are reprogrammed to match the paternal. Here, we provide the mechanism by demonstrating that "Placeholder" nucleosomes, containing histone H2A variant H2A.Z(FV) and H3K4me1, virtually occupy all regions lacking DNA methylation in both sperm and cleavage embryos and reside at promoters encoding housekeeping and early embryonic transcription factors. Upon genome-wide transcriptional onset, genes with Placeholder become either active (H3K4me3) or silent (H3K4me3/K27me3). Notably, perturbations causing Placeholder loss confer DNA methylation accumulation, whereas acquisition/expansion of Placeholder confers DNA hypomethylation and improper gene activation. Thus, during transcriptionally quiescent gametic and embryonic stages, an H2A.Z(FV)/H3K4me1-containing Placeholder nucleosome deters DNA methylation, poising parental genes for either gene-specific activation or facultative repression.


Subject(s)
Cellular Reprogramming/genetics , DNA Methylation/genetics , Embryo, Nonmammalian/metabolism , Germ Cells/metabolism , Nucleosomes/metabolism , Animals , Histones/metabolism , Male , Mutation/genetics , Spermatozoa/metabolism , Zebrafish/genetics , Zebrafish Proteins/metabolism
9.
Cell ; 170(3): 483-491.e8, 2017 Jul 27.
Article in English | MEDLINE | ID: mdl-28735752

ABSTRACT

The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel evolved from an ATP-binding cassette transporter. CFTR channel gating is strictly coupled to phosphorylation and ATP hydrolysis. Previously, we reported essentially identical structures of zebrafish and human CFTR in the dephosphorylated, ATP-free form. Here, we present the structure of zebrafish CFTR in the phosphorylated, ATP-bound conformation, determined by cryoelectron microscopy to 3.4 Å resolution. Comparison of the two conformations shows major structural rearrangements leading to channel opening. The phosphorylated regulatory domain is disengaged from its inhibitory position; the nucleotide-binding domains (NBDs) form a "head-to-tail" dimer upon binding ATP; and the cytoplasmic pathway, found closed off in other ATP-binding cassette transporters, is cracked open, consistent with CFTR's unique channel function. Unexpectedly, the extracellular mouth of the ion pore remains closed, indicating that local movements of the transmembrane helices can control ion access to the pore even in the NBD-dimerized conformation.


Subject(s)
Cystic Fibrosis Transmembrane Conductance Regulator/chemistry , Zebrafish Proteins/chemistry , Adenosine Triphosphate/metabolism , Amino Acid Sequence , Cryoelectron Microscopy , Cystic Fibrosis Transmembrane Conductance Regulator/metabolism , Humans , Models, Molecular , Protein Domains , Sequence Alignment , Zebrafish Proteins/metabolism
10.
Cell ; 167(6): 1586-1597.e9, 2016 Dec 01.
Article in English | MEDLINE | ID: mdl-27912062

ABSTRACT

The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel evolved from the ATP-binding cassette (ABC) transporter family. In this study, we determined the structure of zebrafish CFTR in the absence of ATP by electron cryo-microscopy to 3.7 Å resolution. Human and zebrafish CFTR share 55% sequence identity, and 42 of the 46 cystic-fibrosis-causing missense mutational sites are identical. In CFTR, we observe a large anion conduction pathway lined by numerous positively charged residues. A single gate near the extracellular surface closes the channel. The regulatory domain, dephosphorylated, is located in the intracellular opening between the two nucleotide-binding domains (NBDs), preventing NBD dimerization and channel opening. The structure also reveals why many cystic-fibrosis-causing mutations would lead to defects either in folding, ion conduction, or gating and suggests new avenues for therapeutic intervention.


Subject(s)
Cystic Fibrosis Transmembrane Conductance Regulator/chemistry , Zebrafish Proteins/chemistry , Zebrafish/metabolism , Animals , Cryoelectron Microscopy , Cystic Fibrosis/genetics , Cystic Fibrosis/metabolism , Cystic Fibrosis Transmembrane Conductance Regulator/genetics , Cystic Fibrosis Transmembrane Conductance Regulator/metabolism , Humans , Models, Molecular , Mutation , Protein Folding , Sequence Homology, Amino Acid , Zebrafish Proteins/metabolism
11.
Cell ; 165(1): 139-152, 2016 Mar 24.
Article in English | MEDLINE | ID: mdl-27015311

ABSTRACT

A zebrafish genetic screen for determinants of susceptibility to Mycobacterium marinum identified a hypersusceptible mutant deficient in lysosomal cysteine cathepsins that manifests hallmarks of human lysosomal storage diseases. Under homeostatic conditions, mutant macrophages accumulate undigested lysosomal material, which disrupts endocytic recycling and impairs their migration to, and thus engulfment of, dying cells. This causes a buildup of unengulfed cell debris. During mycobacterial infection, macrophages with lysosomal storage cannot migrate toward infected macrophages undergoing apoptosis in the tuberculous granuloma. The unengulfed apoptotic macrophages undergo secondary necrosis, causing granuloma breakdown and increased mycobacterial growth. Macrophage lysosomal storage similarly impairs migration to newly infecting mycobacteria. This phenotype is recapitulated in human smokers, who are at increased risk for tuberculosis. A majority of their alveolar macrophages exhibit lysosomal accumulations of tobacco smoke particulates and do not migrate to Mycobacterium tuberculosis. The incapacitation of highly microbicidal first-responding macrophages may contribute to smokers' susceptibility to tuberculosis.


Subject(s)
Disease Susceptibility , Lysosomes/metabolism , Macrophages/immunology , Macrophages/pathology , Mycobacterium Infections/immunology , Mycobacterium Infections/pathology , Animals , Granuloma/metabolism , Macrophages/cytology , Macrophages, Alveolar/immunology , Mycobacterium marinum , Pulmonary Alveoli/immunology , Smoking , Transcription Factors/genetics , Transcription Factors/metabolism , Transport Vesicles/metabolism , Tuberculosis/immunology , Tuberculosis/pathology , Zebrafish , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
12.
Nature ; 628(8009): 863-871, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38570687

ABSTRACT

Vertebrate organs require locally adapted blood vessels1,2. The gain of such organotypic vessel specializations is often deemed to be molecularly unrelated to the process of organ vascularization. Here, opposing this model, we reveal a molecular mechanism for brain-specific angiogenesis that operates under the control of Wnt7a/b ligands-well-known blood-brain barrier maturation signals3-5. The control mechanism relies on Wnt7a/b-dependent expression of Mmp25, which we find is enriched in brain endothelial cells. CRISPR-Cas9 mutagenesis in zebrafish reveals that this poorly characterized glycosylphosphatidylinositol-anchored matrix metalloproteinase is selectively required in endothelial tip cells to enable their initial migration across the pial basement membrane lining the brain surface. Mechanistically, Mmp25 confers brain invasive competence by cleaving meningeal fibroblast-derived collagen IV α5/6 chains within a short non-collagenous region of the central helical part of the heterotrimer. After genetic interference with the pial basement membrane composition, the Wnt-ß-catenin-dependent organotypic control of brain angiogenesis is lost, resulting in properly patterned, yet blood-brain-barrier-defective cerebrovasculatures. We reveal an organ-specific angiogenesis mechanism, shed light on tip cell mechanistic angiodiversity and thereby illustrate how organs, by imposing local constraints on angiogenic tip cells, can select vessels matching their distinctive physiological requirements.


Subject(s)
Brain , Neovascularization, Physiologic , Animals , Basement Membrane/metabolism , Blood-Brain Barrier/metabolism , Blood-Brain Barrier/cytology , Brain/cytology , Brain/blood supply , Brain/metabolism , Cell Movement , Collagen Type IV/metabolism , CRISPR-Cas Systems/genetics , Endothelial Cells/metabolism , Endothelial Cells/cytology , Meninges/cytology , Meninges/blood supply , Meninges/metabolism , Organ Specificity , Wnt Proteins/metabolism , Wnt Signaling Pathway , Zebrafish/genetics , Zebrafish/metabolism , Zebrafish Proteins/metabolism , Zebrafish Proteins/genetics
13.
Mol Cell ; 82(5): 986-1002.e9, 2022 03 03.
Article in English | MEDLINE | ID: mdl-35182480

ABSTRACT

Upon fertilization, embryos undergo chromatin reprogramming and genome activation; however, the mechanisms that regulate these processes are poorly understood. Here, we generated a triple mutant for Nanog, Pou5f3, and Sox19b (NPS) in zebrafish and found that NPS pioneer chromatin opening at >50% of active enhancers. NPS regulate acetylation across core histones at enhancers and promoters, and their function in gene activation can be bypassed by recruiting histone acetyltransferase to individual genes. NPS pioneer chromatin opening individually, redundantly, or additively depending on sequence context, and we show that high nucleosome occupancy facilitates NPS pioneering activity. Nucleosome position varies based on the input of different transcription factors (TFs), providing a flexible platform to modulate pioneering activity. Altogether, our results illuminate the sequence of events during genome activation and offer a conceptual framework to understand how pioneer factors interpret the genome and integrate different TF inputs across cell types and developmental transitions.


Subject(s)
Chromatin , Nucleosomes , Animals , Chromatin/genetics , Genome/genetics , Histones/genetics , Histones/metabolism , Nucleosomes/genetics , SOX Transcription Factors/genetics , SOX Transcription Factors/metabolism , Zebrafish/genetics , Zebrafish/metabolism , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
14.
Immunity ; 53(5): 934-951.e9, 2020 11 17.
Article in English | MEDLINE | ID: mdl-33159854

ABSTRACT

Inflammatory signaling is required for hematopoietic stem and progenitor cell (HSPC) development. Here, we studied the involvement of RIG-I-like receptors (RLRs) in HSPC formation. Rig-I or Mda5 deficiency impaired, while Lgp2 deficiency enhanced, HSPC emergence in zebrafish embryos. Rig-I or Mda5 deficiency reduced HSPC numbers by inhibiting inflammatory signals that were in turn enhanced in Lgp2 deficient embryos. Simultaneous reduction of Lgp2 and either Rig-I or Mda5 rescued inflammatory signals and HSPC numbers. Modulating the expression of the signaling mediator Traf6 in RLR deficient embryos restored HSPC numbers. Repetitive element transcripts could be detected in hemogenic endothelial cells and HSPCs, suggesting a role as RLR ligands. Indeed, ectopic expression of repetitive elements enhanced HSPC formation in wild-type, but not in Rig-I or Mda5 deficient embryos. Manipulation of RLR expression in mouse fetal liver HSPCs indicated functional conservation among species. Thus, repetitive elements transcribed during development drive RLR-mediated inflammatory signals that regulate HSPC formation.


Subject(s)
Gene Expression Regulation, Developmental , Hematopoietic Stem Cells/metabolism , Repetitive Sequences, Nucleic Acid , Signal Transduction , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism , Animals , Biomarkers , Chromatin Assembly and Disassembly , DNA Transposable Elements , Disease Susceptibility , Hematopoiesis/genetics , Hematopoietic Stem Cells/cytology , Immunity, Innate , Immunohistochemistry , Inflammation/etiology , Inflammation/metabolism , Inflammation/pathology , RNA Helicases/deficiency , RNA Helicases/genetics , RNA-Binding Proteins/metabolism , TNF Receptor-Associated Factor 6/metabolism , Valproic Acid/pharmacology , Zebrafish
15.
Cell ; 158(2): 263-276, 2014 Jul 17.
Article in English | MEDLINE | ID: mdl-24998929

ABSTRACT

Autism spectrum disorder (ASD) is a heterogeneous disease in which efforts to define subtypes behaviorally have met with limited success. Hypothesizing that genetically based subtype identification may prove more productive, we resequenced the ASD-associated gene CHD8 in 3,730 children with developmental delay or ASD. We identified a total of 15 independent mutations; no truncating events were identified in 8,792 controls, including 2,289 unaffected siblings. In addition to a high likelihood of an ASD diagnosis among patients bearing CHD8 mutations, characteristics enriched in this group included macrocephaly, distinct faces, and gastrointestinal complaints. chd8 disruption in zebrafish recapitulates features of the human phenotype, including increased head size as a result of expansion of the forebrain/midbrain and impairment of gastrointestinal motility due to a reduction in postmitotic enteric neurons. Our findings indicate that CHD8 disruptions define a distinct ASD subtype and reveal unexpected comorbidities between brain development and enteric innervation.


Subject(s)
Child Development Disorders, Pervasive/genetics , Child Development Disorders, Pervasive/physiopathology , DNA-Binding Proteins/genetics , Transcription Factors/genetics , Adolescent , Amino Acid Sequence , Animals , Brain/growth & development , Brain/pathology , Child , Child Development Disorders, Pervasive/classification , Child Development Disorders, Pervasive/pathology , Child, Preschool , DNA-Binding Proteins/metabolism , Female , Gastrointestinal Tract/innervation , Gastrointestinal Tract/physiopathology , Humans , Macaca mulatta , Male , Megalencephaly/pathology , Molecular Sequence Data , Mutation , Sequence Alignment , Transcription Factors/metabolism , Zebrafish , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
16.
Cell ; 157(3): 651-63, 2014 Apr 24.
Article in English | MEDLINE | ID: mdl-24766810

ABSTRACT

Neurodegenerative diseases can occur so early as to affect neurodevelopment. From a cohort of more than 2,000 consanguineous families with childhood neurological disease, we identified a founder mutation in four independent pedigrees in cleavage and polyadenylation factor I subunit 1 (CLP1). CLP1 is a multifunctional kinase implicated in tRNA, mRNA, and siRNA maturation. Kinase activity of the CLP1 mutant protein was defective, and the tRNA endonuclease complex (TSEN) was destabilized, resulting in impaired pre-tRNA cleavage. Germline clp1 null zebrafish showed cerebellar neurodegeneration that was rescued by wild-type, but not mutant, human CLP1 expression. Patient-derived induced neurons displayed both depletion of mature tRNAs and accumulation of unspliced pre-tRNAs. Transfection of partially processed tRNA fragments into patient cells exacerbated an oxidative stress-induced reduction in cell survival. Our data link tRNA maturation to neuronal development and neurodegeneration through defective CLP1 function in humans.


Subject(s)
Cerebellum/growth & development , Cerebellum/pathology , Cleavage And Polyadenylation Specificity Factor/metabolism , Nuclear Proteins/genetics , Phosphotransferases/genetics , RNA Splicing , RNA, Transfer/genetics , Transcription Factors/genetics , Zebrafish Proteins/metabolism , Animals , Brain/metabolism , Brain/pathology , Cleavage And Polyadenylation Specificity Factor/genetics , Female , Humans , Male , Mice , Models, Molecular , Neurodegenerative Diseases/genetics , Neurodegenerative Diseases/pathology , Nuclear Proteins/metabolism , Pedigree , Phosphotransferases/metabolism , RNA, Transfer/metabolism , Saccharomyces cerevisiae/metabolism , Transcription Factors/metabolism , Zebrafish , Zebrafish Proteins/genetics
17.
Nature ; 613(7942): 153-159, 2023 01.
Article in English | MEDLINE | ID: mdl-36517597

ABSTRACT

Sequential segmentation creates modular body plans of diverse metazoan embryos1-4. Somitogenesis establishes the segmental pattern of the vertebrate body axis. A molecular segmentation clock in the presomitic mesoderm sets the pace of somite formation4. However, how cells are primed to form a segment boundary at a specific location remains unclear. Here we developed precise reporters for the clock and double-phosphorylated Erk (ppErk) gradient in zebrafish. We show that the Her1-Her7 oscillator drives segmental commitment by periodically lowering ppErk, therefore projecting its oscillation onto the ppErk gradient. Pulsatile inhibition of the ppErk gradient can fully substitute for the role of the clock, and kinematic clock waves are dispensable for sequential segmentation. The clock functions upstream of ppErk, which in turn enables neighbouring cells to discretely establish somite boundaries in zebrafish5. Molecularly divergent clocks and morphogen gradients were identified in sequentially segmenting species3,4,6-8. Our findings imply that versatile clocks may establish sequential segmentation in diverse species provided that they inhibit gradients.


Subject(s)
Body Patterning , Extracellular Signal-Regulated MAP Kinases , Periodicity , Somites , Zebrafish Proteins , Zebrafish , Animals , Basic Helix-Loop-Helix Transcription Factors/metabolism , Gene Expression Regulation, Developmental , Somites/drug effects , Somites/embryology , Somites/enzymology , Somites/metabolism , Zebrafish/embryology , Zebrafish/metabolism , Zebrafish Proteins/antagonists & inhibitors , Zebrafish Proteins/metabolism , Biological Clocks , Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors , Extracellular Signal-Regulated MAP Kinases/metabolism
18.
Mol Cell ; 81(24): 4979-4993.e7, 2021 12 16.
Article in English | MEDLINE | ID: mdl-34798058

ABSTRACT

The characteristics of the sleep drivers and the mechanisms through which sleep relieves the cellular homeostatic pressure are unclear. In flies, zebrafish, mice, and humans, DNA damage levels increase during wakefulness and decrease during sleep. Here, we show that 6 h of consolidated sleep is sufficient to reduce DNA damage in the zebrafish dorsal pallium. Induction of DNA damage by neuronal activity and mutagens triggered sleep and DNA repair. The activity of the DNA damage response (DDR) proteins Rad52 and Ku80 increased during sleep, and chromosome dynamics enhanced Rad52 activity. The activity of the DDR initiator poly(ADP-ribose) polymerase 1 (Parp1) increased following sleep deprivation. In both larva zebrafish and adult mice, Parp1 promoted sleep. Inhibition of Parp1 activity reduced sleep-dependent chromosome dynamics and repair. These results demonstrate that DNA damage is a homeostatic driver for sleep, and Parp1 pathways can sense this cellular pressure and facilitate sleep and repair activity.


Subject(s)
Behavior, Animal , Brain , DNA Damage , DNA Repair , Neurons , Poly (ADP-Ribose) Polymerase-1 , Sleep , Zebrafish Proteins , Animals , Female , Male , Animals, Genetically Modified , Brain/enzymology , Brain/pathology , Brain/physiopathology , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Ku Autoantigen/genetics , Ku Autoantigen/metabolism , Mice, Inbred C57BL , Neurons/enzymology , Neurons/pathology , Poly (ADP-Ribose) Polymerase-1/genetics , Poly (ADP-Ribose) Polymerase-1/physiology , Rad52 DNA Repair and Recombination Protein/genetics , Rad52 DNA Repair and Recombination Protein/metabolism , Time Factors , Zebrafish/embryology , Zebrafish/genetics , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
19.
EMBO J ; 43(10): 1990-2014, 2024 May.
Article in English | MEDLINE | ID: mdl-38605226

ABSTRACT

Prenatal lethality associated with mouse knockout of Mettl16, a recently identified RNA N6-methyladenosine (m6A) methyltransferase, has hampered characterization of the essential role of METTL16-mediated RNA m6A modification in early embryonic development. Here, using cross-species single-cell RNA sequencing analysis, we found that during early embryonic development, METTL16 is more highly expressed in vertebrate hematopoietic stem and progenitor cells (HSPCs) than other methyltransferases. In Mettl16-deficient zebrafish, proliferation capacity of embryonic HSPCs is compromised due to G1/S cell cycle arrest, an effect whose rescue requires Mettl16 with intact methyltransferase activity. We further identify the cell-cycle transcription factor mybl2b as a directly regulated by Mettl16-mediated m6A modification. Mettl16 deficiency resulted in the destabilization of mybl2b mRNA, likely due to lost binding by the m6A reader Igf2bp1 in vivo. Moreover, we found that the METTL16-m6A-MYBL2-IGF2BP1 axis controlling G1/S progression is conserved in humans. Collectively, our findings elucidate the critical function of METTL16-mediated m6A modification in HSPC cell cycle progression during early embryonic development.


Subject(s)
Hematopoietic Stem Cells , Methyltransferases , RNA Methylation , RNA-Binding Proteins , Transcription Factors , Zebrafish , Animals , Humans , Mice , Adenosine/analogs & derivatives , Adenosine/metabolism , Adenosine/genetics , Cell Cycle , Cell Cycle Proteins/metabolism , Cell Cycle Proteins/genetics , Cell Proliferation , Embryonic Development/genetics , Gene Expression Regulation, Developmental , Hematopoietic Stem Cells/metabolism , Hematopoietic Stem Cells/cytology , Methyltransferases/metabolism , Methyltransferases/genetics , RNA-Binding Proteins/metabolism , RNA-Binding Proteins/genetics , Zebrafish/metabolism , Zebrafish/embryology , Zebrafish/genetics , Zebrafish Proteins/metabolism , Zebrafish Proteins/genetics , Transcription Factors/genetics , Transcription Factors/metabolism , RNA Methylation/genetics
20.
Immunity ; 51(1): 50-63.e5, 2019 07 16.
Article in English | MEDLINE | ID: mdl-31174991

ABSTRACT

Chronic inflammatory diseases are associated with altered hematopoiesis that could result in neutrophilia and anemia. Here we report that genetic or chemical manipulation of different inflammasome components altered the differentiation of hematopoietic stem and progenitor cells (HSPC) in zebrafish. Although the inflammasome was dispensable for the emergence of HSPC, it was intrinsically required for their myeloid differentiation. In addition, Gata1 transcript and protein amounts increased in inflammasome-deficient larvae, enforcing erythropoiesis and inhibiting myelopoiesis. This mechanism is evolutionarily conserved, since pharmacological inhibition of the inflammasome altered erythroid differentiation of human erythroleukemic K562 cells. In addition, caspase-1 inhibition rapidly upregulated GATA1 protein in mouse HSPC promoting their erythroid differentiation. Importantly, pharmacological inhibition of the inflammasome rescued zebrafish disease models of neutrophilic inflammation and anemia. These results indicate that the inflammasome plays a major role in the pathogenesis of neutrophilia and anemia of chronic diseases and reveal druggable targets for therapeutic interventions.


Subject(s)
Anemia/immunology , Fish Diseases/immunology , GATA1 Transcription Factor/metabolism , Inflammasomes/metabolism , Inflammation/immunology , Neutrophils/immunology , Zebrafish Proteins/metabolism , Zebrafish/physiology , Animals , Animals, Genetically Modified , Caspase 1/genetics , Caspase 1/metabolism , Cell Differentiation , Erythroid Cells/cytology , GATA1 Transcription Factor/genetics , Gene Expression Regulation, Developmental , Hematopoiesis , Humans , Inflammasomes/genetics , K562 Cells , Male , Mice , Mice, Inbred C57BL , Proteolysis , Zebrafish Proteins/genetics
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