RESUMEN
The NLRP3 inflammasome responds to microbes and danger signals by processing and activating proinflammatory cytokines, including interleukin 1ß (IL-1ß) and IL-18. We found here that activation of the NLRP3 inflammasome was restricted to interphase of the cell cycle by NEK7, a serine-threonine kinase previously linked to mitosis. Activation of the NLRP3 inflammasome required NEK7, which bound to the leucine-rich repeat domain of NLRP3 in a kinase-independent manner downstream of the induction of mitochondrial reactive oxygen species (ROS). This interaction was necessary for the formation of a complex containing NLRP3 and the adaptor ASC, oligomerization of ASC and activation of caspase-1. NEK7 promoted the NLRP3-dependent cellular inflammatory response to intraperitoneal challenge with monosodium urate and the development of experimental autoimmune encephalitis in mice. Our findings suggest that NEK7 serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division.
Asunto(s)
Proteínas Portadoras/inmunología , Macrófagos/inmunología , Mitosis/inmunología , Proteínas Serina-Treonina Quinasas/inmunología , Animales , Apoptosis , Proteínas Reguladoras de la Apoptosis , Proteínas Adaptadoras de Señalización CARD , Proteínas Portadoras/genética , Caspasa 1 , Cromatografía en Gel , Ensayo de Unidades Formadoras de Colonias , Citocinas , Proteínas del Citoesqueleto , Células Dendríticas , Encefalomielitis Autoinmune Experimental/inmunología , Femenino , Citometría de Flujo , Células HEK293 , Humanos , Inmunoprecipitación , Técnicas In Vitro , Inflamasomas/genética , Inflamasomas/inmunología , Macrófagos Peritoneales/inmunología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Mitocondrias/metabolismo , Monocitos , Quinasas Relacionadas con NIMA , Proteína con Dominio Pirina 3 de la Familia NLR , Proteínas Serina-Treonina Quinasas/genética , Especies Reactivas de Oxígeno , Médula Espinal/inmunologíaRESUMEN
Integrating anatomic nomenclature with geometric anatomic data via a web interface and providing illustration and visualization tools presents numerous challenges. We have developed a library of anatomic models and methods for navigating anatomic nomenclature. Based on the library and tools, we have developed a simple, yet powerful, open web-based tool that uses tree navigation and selection for assembling and downloading self-documenting anatomic scenes in Virtual Reality Modeling Language.
Asunto(s)
Internet , Modelos Anatómicos , Terminología como Asunto , Simulación por Computador , Humanos , Almacenamiento y Recuperación de la Información , Lenguajes de Programación , Estados Unidos , Interfaz Usuario-ComputadorRESUMEN
Creating a library of binary segmentation mask sequences for the abdominal anatomy hierarchy of SNOMED and developing a quick, flexible automatic method for generating iso-surface models from these named structure masks has been a primary goal in our research. This paper describes our development of a clear path for computing visualizations of arbitrary groups of organs from these masks (typically generated from Visible Human data). One use of these methods is teaching the anatomy of various organ systems in the human body using virtual reality environments.
Asunto(s)
Simulación por Computador , Diagnóstico por Imagen/instrumentación , Modelos Anatómicos , Cuerpo Humano , Imagenología Tridimensional , Bibliotecas Médicas , Systematized Nomenclature of Medicine , Estados UnidosRESUMEN
BACKGROUND: Understanding the spatial relationships among the liver segments, and intrahepatic portal and hepatic veins is essential for surgical treatment of liver diseases. Teleimmersive virtual reality enables improved visualization over conventional media because it supports stereo vision, viewer-centered perspective, large angles of view, and interactivity with remote locations. We report a successful pilot study teaching hepatic surgical principles using teleimmersion. METHODS: We developed a teleimmersive environment for teaching with biomedical models including virtual models of the liver segments and portal and hepatic veins. Using the environment, 1 instructor gave a workshop to 6 senior general surgery residents at 2 physical locations. A 24-question (36-point) examination was administered before and after the workshop. RESULTS: The workshop produced significant improvements in the mean test scores between the pretests and posttests (17.67 to 23.67, P <.02). We found no differences between residents who were with the instructor and those at the remote location. Six-month delayed testing demonstrated complete retention of new knowledge. CONCLUSIONS: The teleimmersive environment enabled surgeons to overcome some of the barriers to teaching complex surgical anatomic principles. Using teleimmersive environments, surgical educators and trainees can interact from locations worldwide using virtual anatomic information to achieve their educational goals.