An Overlooked Entry Pathway of Microplastics into Agricultural Soils from Application of Sludge-Based Fertilizers.

The widespread application of sewage sludge produced from wastewater treatment plants for agricultural use has been regarded as a primary source of microplastics (MPs) into soils. However, little is known regarding MPs in sludge-based fertilizers and their relevant fate in soils as being applied in agriculture. We comprehensively investigated the abundance, polymer size, type, and morphology of MPs in dewatered sludge, sewage sludge composts, sludge-based fertilizer-amended soils, and earthworms by stereoscopy and micro Fourier transform infrared (µ-FTIR) spectrometry methods. The results clearly showed that the quantity of MPs in soils exhibited a close correlation with the application rate of sludge-based fertilizers. The total abundances of MPs were 545.9 and 87.6 items/kg in soils after annual amendment with 30 (field A) and 15 t/ha (field B) of sludge composts, which is significantly higher than that without compost application (field C, 5.0 items/kg). Correspondingly, MPs were found in earthworms with low quantities of 1.8 and 0.4 items/individual in fields A and B, respectively, while no MP was detected in field C. We speculate that sludge composts may act as a vehicle of MPs into soils and then enter soil biota and in turn influence the spread of MPs in the environment.

The Effects and Mechanisms of Periplaneta americana Extract Reversal of Multi-Drug Resistance in BEL-7402/5-FU Cells.

The present study reports the reversing effects of extracts from P. americana on multidrug resistance of BEL-7402/5-FU cells, as well as a preliminary investigation on their mechanism of action. A methylthiazolyl tetrazolium (MTT) method was applied to determine the multidrug resistance of BEL-7402/5-FU, while an intracellular drug accumulation assay was used to evaluate the effects of a column chromatography extract (PACC) and defatted extract (PADF) from P. americana on reversing multi-drug resistance. BEL-7402/5-FU reflected high resistance to 5-FU; PACC and PADF could promote drug accumulation in BEL-7402/5-FU cells, among which PADF was more effective than PACC. Moreover, results from the immunocytochemical method showed that PACC and PADF could downregulate the expression of drug resistance-associated proteins (P-gp, MRP, LRP); PACC and PADF had no effects on the expression of multidrug resistance-associated enzymes (GST-π), but PACC could increase the expression of multidrug resistance-associated enzymes (PKC). Results of real-time fluorescence quantitative PCR revealed that PACC and PADF were able to markedly inhibit the expression of multidrug resistance-associated genes (MDR1, LRP and MRP1); PACC presented a significant impact on the gene expression of multidrug resistance-associated enzymes, which increased the gene expression of GST-π and PKC. However, PADF had little impact on the expression of multidrug resistance-associated enzymes. These results demonstrated that PACC and PADF extracted from P. americana could effectively reverse MDR in BEL-7402/5-FU cells, whose mechanism was to inhibit the expression of P-gp, MRP, and LRP, and that PADF was more effective in the reversal of MDR than did PACC. In addition, some of extracts from P. americana altered (sometimes increasing) the expression of multidrug resistance-associated enzymes.

Effects of abiotic mineral transformation of FeS on the dynamic immobilization of Cr(VI) in oxic aquatic environments.

Iron sulfide (FeS) can reductively convert soluble Cr(VI) into insoluble Cr(III) under anoxic conditions. However, the fate and transformation of FeS and the stability of immobilized Cr under various oxic environmental conditions are poorly understood. The results show that FeS transforms into pyrrhotite and pyrite intermediates principally and finally lepidocrocite and elemental sulfur, accordingly accounting for 66.1% and 33.9%. Temperature, fulvic acid as natural organic matter and coexisted ions of nitrate, bicarbonate, and calcium affect the evolution of FeS insignificantly. Transformation of FeS involves surface-mediated oxidation of FeS solids, and minor proton-promoted dissolution and oxidation, accompanying synergistic oxidation of Fe(II) and S(-II). Cr(VI) removal performances of oxygenated FeS with increasing duration showed a rise-fall trend. Reduction dominates Cr(VI) uptake first and finally, sorption prevails with the gradual FeS oxygenation. Cr(VI) removal correlates linearly with Cr(VI) reduction, and the reduced Cr species can be predicted based on the known Cr(VI) removal performance. As the FeS oxygenation time increases, newly generated pyrite improves Cr(VI) reduction and removal, and then a decreasing ability to reduce Cr(VI) causes a drop in Cr(VI) removal. These findings provide new insight into the oxidative transformation of FeS in oxic aquatic environments and its impact on Cr(VI) levels.

Trichloroethylene remediation using zero-valent iron with kaolin clay, activated carbon and bacteria.

Nanoscale particles of zero-valent iron were used to form a permeable reactive barrier whose performance in dechlorinating a solution of trichloroethylene was compared with that of a barrier formed from limestone. The iron was combined with kaolin by calcination. The test liquid contained sewage sludge, and also added NH4Cl and KH2PO4. The average removal rates of trichloroethylene and phosphorus over 365 days both exceeded 94%. Chemical oxygen demand was reduced by 92% and ammonium nitrogen by 43.6%. All were significantly greater than the removals with the limestone barrier. The ceramsite barrier retained 85% of its effectiveness even after 365 days of use. Dechloromonas sp. was the main dechlorinating bacterium, but its removal ability is limited. The removal of trichloroethylene in such a barrier mainly depends on reduction by the zero-valent iron and biodegradation. The results show that the prepared ceramsite is stable and effective in removing trichloroethylene from water. It is a promising in-situ remediation material for groundwater.

Microplastics in freshwater and wild fishes from Lijiang River in Guangxi, Southwest China.

Microplastics (MPs) are ubiquitous contaminants of emerging concern that have gained great attention recently due to their widespread appearance in the environment and potential adverse effects on living biota. Lijiang in Guangxi in China is a world-famous place of tourist attraction and attracted thousands of visitors every year. However, little is known regarding occurrence and distribution of MPs in freshwater and wild fishes in the Lijiang River. In this study, we used stereoscopy and micro Fourier transform infrared spectrometry (µ-FTIR) methods to investigate the abundance, morphotype, size distribution, and polymer type of MPs in freshwater collected by plankton nets and bulk sampling by pumping and filtration. Results showed that abundance of MPs in freshwater with bulk sampling by pumping (67.5 ± 65.6 items/m3) was significantly higher than those using plankton nets (0.67 ± 0.41 items/m3 and 0.15 ± 0.15 items/m3 for mesh sizes of 75 µm and 300 µm, respectively). An average abundance of MPs detected in wild fishes was 0.6 ± 0.6 items/individual, of which, a majority was found in the gastrointestinal tracts. Large-sized (>0.3 mm) and colored MPs in morphotypes of flakes and fibers dominated in both freshwater and wild fishes. Polypropylene-polyethylene copolymer and polyethylene were the top two abundant polymer types of MPs in freshwater, while polyethylene terephthalate dominated in wild fishes. This study provides evidences for our better understanding of pollution status of MPs in the Lijiang River.

Distribution of microplastics in surface water and sediments of Qin river in Beibu Gulf, China.

As a series of pollutants, microplastics have recently attracted widespread attention worldwide. In this study, different surface water and sediment samples were collected at 12 sampling sites along the urban section of Qin River in Guangxi Province, China. Water samples were collected using plankton nets with two different pore sizes (75 µm and 300 µm) and Teflon pump system with 25 µm mesh screen. The results showed that there was no significant difference in the abundance, particle size, and type of microplastics (F = 0.302, P = 0.588) between the two plankton nets, but the microplastic abundance in the samples collected by the Teflon pump was significantly higher (F = 9.12, P = 0.001), especially for microplastic fibers (F = 9.525, P = 0.001). Compared with the plankton net sampling method, the Teflon pump sampling had a smaller sample volume, resulting in a higher estimate of microplastic abundance in the water column. The microplastic abundance in the sediments was 0-97 items·kg-1 dry weight. PE and PP were the main types of microplastics in Qin River, accounting for 67.2% and 72.3% in the water column and sediments, respectively. The microplastics in the water column and sediments were dominated by large-sized particles in the range of 1-5 mm. The sources of microplastic pollution in Qin River may be affected by many factors, including population density, industrial structure, and aquaculture activities. However, aquaculture activities were generally the main source of microplastic pollution in Qin River estuary. Wastewater treatment plants (WWTPs) could effectively decrease the microplastic abundance in urban sewage, reducing the impact of urban sewage discharge on microplastic accumulation in Qin River.

[Impact of a Sewage Treatment Plant on the Accumulation of Microplastics in Freshwater Organisms in the Lijiang River of the Guilin Urban Section].

Microplastics (MPs, particle size<5 mm), as a new pollutant, have attracted wide attention in recent years. The distributions of MPs in effluent of a sewage treatment plant (STP) were examined. Surface water, sediment, and freshwater organism samples were taken from the STP discharge outlet in the Lijiang River tributary (S1), the confluence of tributaries and main streams in the Lijiang River (S2), and downstream locations in the Lijiang River (S3). The impact of STP discharge effluent on the characteristics and spatial distribution of MPs pollution in freshwater organisms was studied. The results showed that the freshwater organisms had a probability of uptake of MPs by 94.2%. The mean abundance of MPs in S1 (2.7 n·ind-1) was significantly higher than that of S3 (1.9 n·ind-1, P<0.05). The MPs found in S1 and S3 were mainly <0.10 mm, accounting for 46.0% and 30.5%, respectively. The fiber type of MPs was observed in the body of freshwater organisms. Polyethylene terephthalate was the major polymer form in S1 organisms, while polypropylene was the major polymer form in S3. The effluent discharged from the STP led to the accumulation of MPs in freshwater organisms.

[Automobile versus pedestrian accidents analysis by fixed-parameters computer simulation].

OBJECTIVE: Using computer simulation to analyze the effects of speed, type of automobile and impacted position on crash-course and injuries of pedestrians in automobile vs. pedestrian accidents. METHODS: Automobiles (bus, minibus, car and truck) and pedestrian models were constructed with multi-body dynamics computing method. The crashes were simulated at different impact speeds (20, 30, 40, 50 and 60 km/h) and different positions (front, lateral and rear of pedestrians). Crash-courses and their biomechanical responses were studied. RESULTS: If the type of automobile and impact position were the same, the crash-courses were similar (impact speed < or = 60 km/h). There were some characteristics in the head acceleration, upper neck axial force and leg axial force. CONCLUSION: Multi-body dynamics computer simulation of crash can be applied to analyze crash-course and injuries (head, neck and leg) of pedestrians.