RESUMEN
Anisotropic gradient materials are considered as promising and novel in that they have numerous functional properties and are able to transform into hierarchical microstructures. We report a facile method of gradient inorganic thin film synthesis through diffusion-controlled deposition at the gas-solution interface. To investigate the reaction of interfacial phase boundary controllable hydrolysis by gaseous ammonium, an aqueous solution of FeCl3 and MnCl2 was chosen, as the precipitation pH values for the hydroxides of these metals differ gradually. As a result of synthesis using the gas-solution interface technique (GSIT), a thin film is formed on the surface of the solution that consists of Mn2+(Fe,Mn)23+O4 nanoparticles with hausmannite crystal structure. The ratio between iron and manganese in the film can be adjusted over a wide range by varying the synthetic procedure. Specific conditions are determined that allow the formation of a Mn-Fe mixed oxide film with a gradient of composition, morphology, and properties, as well as its further transformation into microscrolls with a diameter of 10-20 µm and a length of up to 300 µm, showing weak superparamagnetic properties. The technique reported provides a new interfacial route for the development of functional gradient materials with tubular morphology.
RESUMEN
Non-coding RNA expression has shown to have cell type-specificity. The regulatory characteristics of these molecules are impacted by changes in their expression levels. We performed next-generation sequencing and examined small RNA-seq data obtained from 6 different types of blood cells separated by fluorescence-activated cell sorting of severe COVID-19 patients and healthy control donors. In addition to examining the behavior of piRNA in the blood cells of severe SARS-CoV-2 infected patients, our aim was to present a distinct piRNA differential expression portrait for each separate cell type. We observed that depending on the type of cell, different sorted control cells (erythrocytes, monocytes, lymphocytes, eosinophils, basophils, and neutrophils) have altering piRNA expression patterns. After analyzing the expression of piRNAs in each set of sorted cells from patients with severe COVID-19, we observed 3 significantly elevated piRNAs - piR-33,123, piR-34,765, piR-43,768 and 9 downregulated piRNAs in erythrocytes. In lymphocytes, all 19 piRNAs were upregulated. Monocytes were presented with a larger amount of statistically significant piRNA, 5 upregulated (piR-49039 piR-31623, piR-37213, piR-44721, piR-44720) and 35 downregulated. It has been previously shown that piR-31,623 has been associated with respiratory syncytial virus infection, and taking in account the major role of piRNA in transposon silencing, we presume that the differential expression patterns which we observed could be a signal of indirect antiviral activity or a specific antiviral cell state. Additionally, in lymphocytes, all 19 piRNAs were upregulated.
Asunto(s)
COVID-19 , Citometría de Flujo , ARN Interferente Pequeño , SARS-CoV-2 , Humanos , COVID-19/genética , COVID-19/virología , ARN Interferente Pequeño/metabolismo , ARN Interferente Pequeño/genética , SARS-CoV-2/genética , Masculino , Femenino , Persona de Mediana Edad , Monocitos/metabolismo , Adulto , Células Sanguíneas/metabolismo , ARN de Interacción con PiwiRESUMEN
Severe COVID-19 alters the biochemical and morphological characteristics of blood cells in a wide variety of ways. To date, however, the vast majority of research has been devoted to the study of leukocytes, while erythrocyte morphological changes have received significantly less attention. The aim of this research was to identify erythrocyte morphology abnormalities that occur in COVID-19, compare the number of different poikilocyte types, and measure erythrocyte sizes to provide data on size dispersion. Red blood cells obtained from 6 control donors (800-2200 cells per donor) and 5 COVID-19 patients (800-1900 cells per patient) were examined using low-voltage scanning electron microscopy. We did not discover any forms of erythrocyte morphology abnormalities that would be specific to COVID-19. Among COVID-19 patients, we observed an increase in the number of acanthocytes (p = 0.01) and a decrease in the number of spherocytes (p = 0.03). In addition, our research demonstrates that COVID-19 causes an increase in the median (p = 0.004) and interquartile range (p = 0.009) when assessing erythrocyte size. The limitation of our study is a small number of participants.
RESUMEN
We report here the growth and functional properties of silicon-based nanowhisker (NW) diodes produced by the vapor-liquid-solid process using a pulsed laser deposition technique. For the first time, we demonstrate that this method could be employed to control the size and shape of silicon NWs by using a two-component catalyst material (Au/Cu ≈ 60:1). During the NW growth, copper is distributed on the outer surface of the NW, whereas gold sticks as a droplet to its top. The length of NWs is defined by the total amount of copper in the catalyst alloy droplet. The measurements of the electrical transport properties revealed that in contact with the substrate, individual NWs demonstrate typical I-V diode characteristics. Our approach can become an important new tool in the design of novel electronic components.