RESUMEN
In the present study, we analyzed the intracellular accumulation of 6-(methylsulfinyl)hexyl isothiocyanate (6MITC) and its analogs in proinflammatory stimuli-activated J774.1 cells to predict the biological potencies of the ITCs. Our present analyses exhibited that the intracellular accumulation was in the order of 6MITC>2b>2e≈2c>2g>2d>2f>2h. Investigation of reactivity of the ITCs with glutathione (GSH) in the tumor cells revealed partial inhibition of GSH by the ITCs. Furthermore, the inhibition of nitric oxide (NO) production in the tumor cells was ascribed to the intracellularly accumulated ITCs. The NO suppression was correlated with the inhibition of tumor cell growth. Our present results suggest that the intracellular accumulation of the ITCs can be used to predict their biological potencies, such as inhibition of NO production that was correlated with suppression of tumor cell growth. To the best of our knowledge, this is the first report to predict the biological potency of 6MITC and its analogs with their intracellular accumulation.
Asunto(s)
Isotiocianatos/química , Óxido Nítrico/antagonistas & inhibidores , Humanos , Macrófagos/efectos de los fármacos , Óxido Nítrico/biosíntesisRESUMEN
The present study deals with in silico prediction and in vitro evaluation of the selective cytotoxic effects of triterpenoids on tumorigenic human c-Ha-ras and mouse c-myc cotransfected highly metastatic serum-free mouse embryo-1 (r/m HM-SFME-1) cells. Ligand fitting of five different triterpenoids to 11ß-hydroxysteroid dehydrogenase type 2 (11ßHSD2) was analyzed with a molecular modeling method, and glycyrrhetinic acid (GA) was the best-fitted triterpenoid to the ligand binding site in 11ßHSD2. Analysis of antiproliferative effects revealed that GA, oleanolic acid, and ursolic acid had selective toxicity against the tumor cells and that GA was the most potent triterpenoid in its selectivity. The toxic activity of the tested triterpenoids against the tumor cells showed good correlations with the partition coefficient (logP) and polar surface area values. Time-lapse microscopy, fluorescence staining, and confocal laser scanning microscopic observation revealed that GA induced morphologic changes typical of apoptosis such as cell shrinkage and blebbing and also disrupted the cytoskeletal proteins. Furthermore, GA exhibited a strong inhibitory effect on 11ßHSD2 activity in the tumor cells. Our current results suggest that analysis of the ligand-receptor interaction between triterpenoids and 11ßHSD2 can be utilized to predict their antitumor effects and that GA can be used as a possible chemopreventive and therapeutic antitumor agent. To the best of our knowledge, this is the first report on in silico prediction of the toxic effects of triterpenoids on tumor cells by 11ßHSD2 inhibition.
Asunto(s)
11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2/antagonistas & inhibidores , Antineoplásicos/farmacología , Inhibidores Enzimáticos/farmacología , Ácido Glicirretínico/farmacología , Proteínas de Neoplasias/antagonistas & inhibidores , Neoplasias Experimentales/tratamiento farmacológico , 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2/química , 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2/genética , 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2/metabolismo , Animales , Antiinflamatorios/química , Antiinflamatorios/farmacología , Antineoplásicos/química , Línea Celular Tumoral , Ensayos de Selección de Medicamentos Antitumorales/métodos , Inhibidores Enzimáticos/química , Ácido Glicirretínico/química , Humanos , Ligandos , Ratones , Proteínas de Neoplasias/química , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Neoplasias Experimentales/enzimología , Neoplasias Experimentales/genética , Neoplasias Experimentales/patologíaRESUMEN
Inflammatory bowel disease (IBD) describes a set of disorders involving alterations to gastrointestinal physiology and mucosal immunity. Unravelling its complex pathophysiology is important since many IBD patients are refractory to or suffer adverse side effects from current treatments. Isothiocyanates (ITCs), such as 6-(methylsulfinyl)hexyl ITC (6-MITC) in Wasabia japonica, have potential anti-inflammatory activity. We aimed to elucidate the pathways through which 6-MITC alleviates inflammation by examining its role in the nuclear factor-kappa B (NF-κB) pathway through inhibition of glycogen synthase kinase 3 beta (GSK-3ß) using a chemically induced murine model of IBD, cell-based and in silico techniques. The effects of 6-MITC and two NF-κB inhibitors, sulfasalazine (SS), pyrrolidine dithiolcarbamate (PDTC) were investigated on a dextran sulfate sodium (DSS)-induced murine mouse model of acute and chronic colitis using macroscopic measurements and pro-inflammatory markers. The effect of 6-MITC on NF-κB induction was assessed using a murine macrophage cell line. Complexes of GSK-3ß-6-MITC and GSK-3ß-ATP were generated in silico to elucidate the mechanism of 6-MITC's direct inhibition of GSK-3ß. Changes in pro-inflammatory markers, inducible nitric oxide synthase (iNOS) (increased) and interleukin-6 (IL-6) (decreased) demonstrated that iNOS regulation occurred at the translational level. Intraperitoneal (ip) injection of 6-MITC to the colitis-induced mice ameliorated weight loss whereas oral administration had negligible effect. Fecal blood and colon weight/length ratio parameters improved on treatment with 6-MITC and the other NF-κB inhibitors. Levels of NF-κB decreased upon addition of 6-MITC in vitro while structural studies showed 6-MITC acts competitively to inhibit GSK-3ß at the ATP binding site. In this study we demonstrated that 6-MITC inhibits NF-κB signaling via GSK-3ß inhibition ameliorating fecal blood, colonic alterations and DSS-induced weight loss indirectly indicating reduced intestinal stress. Taken together these results suggest a role for 6-MITC in the treatment of IBD acting to alleviate inflammation through the GSK-3ß/NF-κB pathway. Furthermore, the GSK-3ß-6-MITC model can be utilized as a basis for development of novel therapeutics targeting GSK-3ß for use in other disorders including cancer.
Asunto(s)
Antiinflamatorios/química , Glucógeno Sintasa Quinasa 3 beta/antagonistas & inhibidores , Isotiocianatos/química , Wasabia/química , Animales , Antiinflamatorios/metabolismo , Antiinflamatorios/farmacología , Antiinflamatorios/uso terapéutico , Línea Celular , Sulfato de Dextran/toxicidad , Regulación hacia Abajo/efectos de los fármacos , Femenino , Glucógeno Sintasa Quinasa 3 beta/metabolismo , Humanos , Enfermedades Inflamatorias del Intestino/inducido químicamente , Enfermedades Inflamatorias del Intestino/tratamiento farmacológico , Enfermedades Inflamatorias del Intestino/patología , Interleucina-6/metabolismo , Isotiocianatos/metabolismo , Isotiocianatos/farmacología , Isotiocianatos/uso terapéutico , Lipopolisacáridos/farmacología , Macrófagos/citología , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Ratones , Ratones Endogámicos BALB C , FN-kappa B/antagonistas & inhibidores , FN-kappa B/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , Transducción de Señal/efectos de los fármacos , Regulación hacia Arriba/efectos de los fármacos , Wasabia/metabolismoRESUMEN
We analyzed the effects of glycyrrhetinic acid (GA), a licorice compound, on the induction of anoikis-like death and cytoskeletal disruption in the central nervous system (CNS) tumorigenic cells. GA was cytotoxic in time- and dose-dependent manners, and the tumorigenic cells shed floating cells upon the GA treatment and even some of the adherent cells were easily detached from the fibronectin-coated culture dish by gentle shaking and aspiration. Reculture of the detached cells revealed that the longer the duration of GA exposure, the less the number of the proliferatable cells. These results indicate that GA perturbs cell adhesion and induces anoikis-like cell death. Further, GA also induced morphologic changes and disturbed cytoskeletal proteins.
Asunto(s)
Anoicis/efectos de los fármacos , Neoplasias del Sistema Nervioso Central/tratamiento farmacológico , Citoesqueleto/efectos de los fármacos , Ácido Glicirretínico/farmacología , Animales , Anoicis/fisiología , Muerte Celular/efectos de los fármacos , Muerte Celular/fisiología , Neoplasias del Sistema Nervioso Central/patología , Citoesqueleto/patología , Relación Dosis-Respuesta a Droga , Ácido Glicirretínico/uso terapéutico , Humanos , Ratones , Ratones Endogámicos BALB C , Células Tumorales CultivadasRESUMEN
Magnolia species are widely cultivated in Japan as garden plants, and have been found to contain various compounds, including alkaloids, terpenoids, lignans, and neolignans. The constituents of the mature fruits of M. denudata were investigated, and two new phenolic derivatives, named denudalide and denudaquinol, were isolated and characterized, together with a known neolignan compound (denudatin A). Denudalide and denudaquinol showed cytotoxicity against the SFME and r/mHM-SFME-1 cell lines.
Asunto(s)
Frutas/química , Magnolia/química , Fenol/química , Fenol/toxicidad , Línea Celular , Proliferación Celular/efectos de los fármacos , Frutas/crecimiento & desarrollo , Espectroscopía de Resonancia Magnética , Magnolia/crecimiento & desarrollo , Fenol/aislamiento & purificaciónRESUMEN
We analyzed the effects of thiol compounds on the biological activities of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC). Thiol compounds abolished the cytotoxic activity of 6-MITC, but did not abolish its activity augmenting cellular total glutathione levels and gamma-glutamylcysteine ligase gene expression. Thiol compounds might play an important role in the augmentation of several significant biological activities by overcoming the inherent limitations of 6-MITC.
Asunto(s)
Supervivencia Celular/efectos de los fármacos , Glutatión/metabolismo , Isotiocianatos/farmacología , Compuestos de Sulfhidrilo/farmacología , Animales , Línea Celular , RatonesRESUMEN
Denudatin A and B, denudadione B, fargesone A and machilin G were isolated from Magnolia denudata. These compounds showed inhibitory effects on nitric oxide (NO) production in the lipopolysaccharide plus interferon-gamma activated-murine macrophage cell line, J774.1. Some but not all of the inhibition of NO production by machilin G, and denudatin A and B was apparently through the decreased expression of the inducible NO synthase (iNOS) gene.
Asunto(s)
Frutas/química , Lignanos/química , Lignanos/farmacología , Magnolia/química , Óxido Nítrico/antagonistas & inhibidores , Óxido Nítrico/biosíntesis , Animales , Línea Celular , Frutas/crecimiento & desarrollo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Lignanos/clasificación , Lignanos/aislamiento & purificación , Magnolia/crecimiento & desarrollo , Ratones , Estructura Molecular , Óxido Nítrico Sintasa de Tipo II/genética , Óxido Nítrico Sintasa de Tipo II/metabolismoRESUMEN
The effects of bisphenol A (BPA) on the differentiation of serum-free mouse embryo (SFME) cells, the astrocyte progenitor cells in the central nervous system, were examined. SFME cells were exposed to 10 ng/ml leukemia inhibitory factor (LIF) and 10ng/ml bone morphogenetic protein 2 (BMP2) to increase glial fibrillary acidic protein (GFAP) expression and induce cell differentiation. Various concentrations of BPA (0.1 pg/ml-1 microg/ml) were then added to determine their effects on the cell differentiation. SFME cells were effectively differentiated by LIF and BMP2 in completely serum-free cultures. Cell proliferation following cell differentiation was not significantly affected by low-level BPA. However, GFAP expression was significantly increased in SFME cells in the presence of 1-100 pg/ml BPA. These increases were due to excessive activation of signal transducer and activator of transcription 3 (STAT3) and mothers against decapentaplegic homolog 1 (Smad1) by the low-level BPA.
Asunto(s)
Contaminantes Ocupacionales del Aire/farmacología , Astrocitos/metabolismo , Proteína Ácida Fibrilar de la Glía/biosíntesis , Fenoles/farmacología , Factor de Transcripción STAT3/metabolismo , Proteína Smad1/metabolismo , Células Madre/metabolismo , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo , Compuestos de Bencidrilo , Biotransformación/efectos de los fármacos , Western Blotting , Proteína Morfogenética Ósea 2 , Proteínas Morfogenéticas Óseas/metabolismo , Diferenciación Celular/efectos de los fármacos , Línea Celular , Medio de Cultivo Libre de Suero , Receptor alfa de Estrógeno/metabolismo , Receptor beta de Estrógeno/metabolismo , Técnica del Anticuerpo Fluorescente , Interleucina-6/metabolismo , Factor Inhibidor de Leucemia , Ratones , Proteínas del Tejido Nervioso/metabolismo , Factor de Transcripción 2 de los Oligodendrocitos , Proteína smad6/metabolismo , Factor de Crecimiento Transformador beta/metabolismoRESUMEN
We examined both the induction of quinone reductase (QR) by 6-(methylsulfinyl)hexyl isothiocyanate and its cytotoxicity in Hepa1c1c7 cells, and compared the sensitivity of these two responses to NAC. QR activity was increased by 6-(methylsulfinyl)hexyl isothiocyanate in a dose-dependent manner. At 80 microM, the compound was significantly toxic to cells, but the resulting QR inhibition was dose-dependently overcome by NAC. Augmentation of QR activity by 6-(methylsulfinyl)hexyl isothiocyanate seemed to be due to augmented expression of QR mRNA, which was significantly increased by the compound. Inhibition of QR gene expression was seen at 80 microM and could be overcome by NAC. Optimal induction of QR gene expression by the compound (at 40 microM) was slightly but significantly inhibited by 10 mM NAC but not by 1 mM. The present study suggests that induction of Phase 2 detoxification enzymes by isothiocyanate compounds may be further enhanced by suppression of their inherent cytotoxic activity.
Asunto(s)
Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Isotiocianatos/farmacología , NAD(P)H Deshidrogenasa (Quinona)/biosíntesis , Acetilcisteína/farmacología , Animales , Línea Celular Tumoral , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Inducción Enzimática/efectos de los fármacos , Isotiocianatos/antagonistas & inhibidores , Ratones , NAD(P)H Deshidrogenasa (Quinona)/genéticaRESUMEN
The effects of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) on gene expression and synthesis of glial fibrillary acidic protein (GFAP) in differentiation-disrupted serum-free mouse embryo (SFME) cells were examined. SFME cells were exposed to fetal calf serum (FCS) and dimethyl sulfoxide (DMSO) to induce differentiation and increase GFAP synthesis. Disruption of differentiation was caused by low-level toluene, significantly inhibiting GFAP synthesis. TCDD at 0.01, 0.1 and 1 pg/ml in the presence of low-level toluene increased GFAP synthesis in the SFME cells, while expression of GFAP mRNA showed no significant change. The TCDD-treated SFME cells detached from the culture substratum, indicating an apparent change in cell adhesion. These results suggest that low-level TCDD further disrupts differentiation of SFME cells in the presence of low-level toluene by upregulating GFAP synthesis and by altering the ability in cell adhesion and that GFAP synthesis is not disrupted at transcription but at translation.
RESUMEN
Recent findings that describe endocrine disruption caused by exposure to low levels of certain chemicals in the environment have led to a paradigm shift in the way toxicology studies are designed. Toluene at high levels damages the human central nervous system; however, the effects of toluene at low levels have not been studied. The authors used serum-free mouse embryo cells-a precursor of astrocytes-to predict the effect of chemicals on developing brain cells. When serum-free mouse embryo cells were exposed to low levels of toluene, induction of glial fibrillary acidic protein was inhibited. This study demonstrated that environmentally relevant low levels of toluene could disrupt normal prenatal brain development.
Asunto(s)
Contaminantes Atmosféricos/toxicidad , Astrocitos/efectos de los fármacos , Exposición a Riesgos Ambientales/efectos adversos , Tolueno/toxicidad , Animales , Encéfalo/efectos de los fármacos , Encéfalo/embriología , Diferenciación Celular/efectos de los fármacos , Células Cultivadas/efectos de los fármacos , Electroforesis en Gel de Poliacrilamida , Proteína Ácida Fibrilar de la Glía/metabolismo , Immunoblotting , RatonesRESUMEN
In the present study, we performed in silico and in vitro analyses to evaluate the chemosensitizing effects of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) on tumor cells. Our in silico analyses of the ligand-receptor interactions between 6-MITC and the glutamate cysteine ligase (GCL) catalytic subunit (GCLC) revealed that 6-MITC possibly inhibited GCL enzyme activity, and that Cys-249 and Gln-251 were important residues for stable binding of ligands to GCLC. It was further found that 6-MITC interfered with the hydrogen bonds of the cysteinyl and glutamyl moieties of GSH with Cys-249 and Gln-251, respectively, and possibly overrode the feedback inhibition of GCL enzyme activity by GSH. To the best of our knowledge, this is the first in silico analysis to suggest an overriding effect of 6-MITC on GSH-induced feedback inhibition of GCL. In our in vitro analyses, combined treatment with 6-MITC and L-buthionine-S,R-sulfoximine (BSO) depleted GSH within 4 h in tumorigenic human c-Ha-ras and mouse c-myc-cotransfected highly metastatic serum-free mouse embryo-1 (r/m HM-SFME-1) cells, but did not deplete GSH in normal SFME cells. Furthermore, exposure to 6-MITC plus BSO for 4h, followed by glycyrrhetinic acid (GA) treatment for 3h, eradicated the tumor cells with minimal damage to the normal cells. The present findings suggest that 6-MITC in combination therapies could be used to sensitize tumor cells to antitumor agents, thereby leading to their eradication.
Asunto(s)
Antineoplásicos/farmacología , Butionina Sulfoximina/farmacología , Glutamato-Cisteína Ligasa/antagonistas & inhibidores , Ácido Glicirretínico/farmacología , Isotiocianatos/farmacología , Animales , Línea Celular , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Simulación por Computador , Sinergismo Farmacológico , Glutamato-Cisteína Ligasa/metabolismo , Glutatión/metabolismo , Humanos , Ratones , Simulación del Acoplamiento Molecular , Subunidades de Proteína/antagonistas & inhibidores , Subunidades de Proteína/metabolismoRESUMEN
Molecular docking and structural analysis of the cofactor-protein interaction between NAD(+) and human (h) or mouse (m) 11ß-hydroxysteroid dehydrogenase type 2 (11ßHSD2) were performed with the molecular operating environment (MOE). 11ßHSD1 (PDB code: 3HFG) was selected as a template for the 3D structure modeling of 11ßHSD2. The MOE docking (MOE-dock) and the alpha sphere and excluded volume-based ligand-protein docking (ASE-dock) showed that both NAD(+)-h11ßHSD2 and NAD(+)-m11ßHSD2 models have a similar binding orientation to the template cofactor-protein model. Our present study also revealed that Asp91, Phe94, Tyr232 and Thr267 could be of importance in the interaction between NAD(+) and 11ßHSD2. NADP(+) was incapable of entering into the cofactor-binding site of the 11ßHSD2 models. The present study proposes the latest models for 11ßHSD2 and its cofactor NAD(+), and to the best of our knowledge, this is the first report of a m11ßHSD2 model with NAD(+).
Asunto(s)
11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2/química , NAD/química , 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2/metabolismo , Animales , Sitios de Unión , Simulación por Computador , Humanos , Ratones , Estructura Molecular , NAD/metabolismo , Unión ProteicaRESUMEN
11ß-hydroxysteroid dehydrogenase type 2 (11ßHSD2) was homology-modeled by a Boltzmann-weighted randomized modeling procedure, using the X-ray crystal structure of 11ßHSD1 (PDB code: 3HFG) as a template. The model exhibited significant 3D similarities to 11ßHSD1. The contact energy profiles of the 11ßHSD2 model were in good agreement with that of the X-ray structure of 11ßHSD1. The secondary structure of the 11ßHSD2 model exhibited a central 6-stranded all-parallel ß-sheet sandwich-like structure, flanked on both sides by 3-helices. Ramachandran plots revealed that only 1.9% of the amino acid residues were in the disfavored region for 11ßHSD2. Furthermore, the ligand-binding site (LBS) volume was calculated to be 845 Å(3), which suggests that the LBS of 11ßHSD2 is sufficiently large to contain cofactors and substrates (ligands), such as NAD(+) and cortisol. The electrostatic analysis revealed that the 11ßHSD2 model had a positive potential at the LBS, which indicates that 11ßHSD2 possibly attracts negatively charged ligands at the LBS. These results indicate that the model was successfully evaluated and analyzed. Consequently, it is proposed that the 11ßHSD2 model in the present study will be suitable for further in silico structure-based de novo antitumor drug designing. To the best of our knowledge, this is the latest report of an accurate 11ßHSD2 model to target 11ßHSD2 for the development of anticancer drugs.
Asunto(s)
11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2/química , Modelos Moleculares , Homología de Secuencia de Aminoácido , 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2/metabolismo , Secuencia de Aminoácidos , Sitios de Unión , Ligandos , Datos de Secuencia Molecular , Estructura Secundaria de Proteína , Programas Informáticos , Electricidad Estática , Estereoisomerismo , TermodinámicaRESUMEN
Mouse (m) 11ß-hydroxysteroid dehydrogenase type 2 (11ßHSD2) was homology-modeled, and its structure and ligand-receptor interaction were analyzed. The modeled m11ßHSD2 showed significant 3D similarities to the human (h) 11ßHSD1 and 2 structures. The contact energy profiles of the m11ßHSD2 model were in good agreement with those of the h11ßHSD1 and 2 structures. The secondary structure of the m11ßHSD2 model exhibited a central 6-stranded all-parallel ß-sheet sandwich-like structure, flanked on both sides by 3-helices. Ramachandran plots revealed that only 1.1% of the amino acid residues were in the disfavored region for m11ßHSD2. Further, the molecular surfaces and electrostatic analyses of the m11ßHSD2 model at the ligand-binding site exhibited that the model was almost identical to the h11ßHSD2 model. Furthermore, docking simulation and ligand-receptor interaction analyses revealed the similarity of the ligand-receptor bound conformation between the m11ßHSD2 and h11ßHSD2 models. These results indicate that the m11ßHSD2 model was successfully evaluated and analyzed. To the best of our knowledge, this is the first report of a m11ßHSD2 model with detailed analyses, and our data verify that the mouse model can be utilized for application to the human model to target 11ßHSD2 for the development of anticancer drugs.
RESUMEN
With the intensive need for the development of more effective and safer agents for chemoprevention and therapy of human cancer, natural products from plants have been expected to play significant roles in creating new and better chemopreventive and therapeutic agents. Selectivity is also an important issue in cancer prevention and therapy. In the present study, normal serum-free mouse embryo (SFME) and tumorigenic human c-Ha-ras and mouse c-myc cotransfected highly metastatic serum-free mouse embryo-1 (r/m HM-SFME-1) cells were treated with various concentrations of clinically available antitumor agents or glycyrrhetinic acid (GA), and the antiproliferative effects of these compounds were determined by the MTT assay. Western blotting analysis, RT-PCR, fluorescence staining and confocal laser scanning microscopic observation were adopted to analyze H-Ras regulation. GA exhibited the tumor cell-selective toxicity through H-Ras downregulation, and its selectivity was superior to those of all the clinically available antitumor agents examined. For the selective toxicity of tumor cells, GA was most effective at 10 microM. Interestingly, this concentration was the same as the previously reported maximum plasma GA level reached in humans ingesting licorice. These results in the present study suggest that GA with its cytotoxic effects could be utilized as a promising chemopreventive and therapeutic antitumor agent.
Asunto(s)
Antineoplásicos/farmacología , Genes ras/efectos de los fármacos , Ácido Glicirretínico/farmacología , Neoplasias Experimentales/tratamiento farmacológico , Animales , Antibióticos Antineoplásicos/farmacología , Western Blotting , Proliferación Celular/efectos de los fármacos , Regulación hacia Abajo/efectos de los fármacos , Doxorrubicina/farmacología , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Humanos , Ratones , Neoplasias Experimentales/metabolismo , Polienos/farmacología , Alcamidas Poliinsaturadas/farmacología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células Tumorales CultivadasRESUMEN
Licorice extracts are used worldwide in foods and medicines, and glycyrrhetinic acid (GA) is a licorice component that has been reported to induce various important biological activities. In the present study, we show that GA induces actin disruption and has tumor cell-selective toxic properties, and that its selectivity is superior to those of all the clinically available antitumor agents tested. The cytotoxic activity of GA and the tested antitumor agents showed better correlation with the partition coefficient (log P) values rather than the polar surface area (PSA) values. For selective toxicity against tumor cells, GA was most effective at 10 microM that was the same concentration as the previously reported maximum plasma GA level reached in humans ingesting licorice. These results suggest that GA could be utilized as a promising chemopreventive and therapeutic antitumor agent. The underlying mechanisms involved in the selective toxicity to tumor cells by GA are also preliminarily discussed.
Asunto(s)
Actinas/metabolismo , Antineoplásicos/farmacología , Sistema Nervioso Central/patología , Ácido Glicirretínico/farmacología , Neoplasias/patología , Células Madre/efectos de los fármacos , Células Madre/patología , Animales , Antineoplásicos/toxicidad , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Genes myc/genética , Genes ras/genética , Ácido Glicirretínico/toxicidad , Humanos , Ratones , Células Madre/metabolismo , TransfecciónRESUMEN
AIM: We have reported the in vitro and in vivo anticancer activities of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) derived from a Japanese spice, wasabi. In order to obtain some clues about the mechanism of the anticancer activity, we have studied the effect of alkyl isothiocyanates (MITCs) on protein kinase activities. METHODS: The anti-autophosphorylation activity of MITCs with respect to the epidermal growth factor (EGF)-stimulated receptor kinase of A431 epidermoid carcinoma cells was examined by incorporation of radioactive ATP into an acid-insoluble fraction. Their anti-phosphorylation activity with respect to the non-receptor protein kinase was analyzed by a standard SDS-PAGE method. RESULTS: All the tested MITCs interfered with the EGF-stimulated receptor kinase activity in a dose-dependent manner, although their effects were less than 1/10 of that of erbstatin in microg/ml. On the other hand, the MITCs did not interfere with non-receptor kinases (kinase A, kinase C, tyrosine kinase and calmodulin dependent kinase III), but enhanced non-receptor tyrosine kinase. DISCUSSION: A possible anticancer mechanism of MITCs may involve the suppression of EGF receptor kinase activity and augmentation of non-receptor PTK.
Asunto(s)
Antineoplásicos/farmacología , Receptores ErbB/efectos de los fármacos , Isotiocianatos/farmacología , Proteínas Tirosina Quinasas/efectos de los fármacos , Antineoplásicos/administración & dosificación , Antineoplásicos/química , Carcinoma de Células Escamosas/metabolismo , Línea Celular Tumoral , Relación Dosis-Respuesta a Droga , Electroforesis en Gel de Poliacrilamida , Receptores ErbB/metabolismo , Humanos , Hidroquinonas/farmacología , Isotiocianatos/administración & dosificación , Isotiocianatos/química , Células K562 , Fosforilación/efectos de los fármacos , Proteínas Tirosina Quinasas/metabolismoRESUMEN
There is increasing demand for novel anti-inflammatory drugs with different mechanisms of action. We synthesized a series of isothiocyanates 2b-h based on 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) found in the pungent spice Wasabia japonica. Inhibitory activities against in-vitro growth of tumor cells and production of nitric oxide (NO) using the mouse macrophage-like cell line J774.1 were noted. All isothiocyanates were optimized by Hartree-Fock/3-21G model, and the logP values and the polar surface area (PSA) values were calculated. Substitution of the methylsulfinyl group (CH(3)S(O)-R) in 6-MITC with a formyl (CHO-R), a methylsulfanyl (CH(2)S-R) or a methyl (CH(3)-R) group reduced the activities of the parent isothiocyanate. Substitution with a formyl group resulted in lower lipophilicity (logP value) whereas substitution with a methylsulfanyl or methyl group resulted in a lower PSA value. The inhibitory activity of isothiocyanates showed better correlation with their PSA values rather than their partition coefficient (logP) values. Isothiocyanates with higher PSA values and some degree of logP value may have potent biological activity.
Asunto(s)
Antiinflamatorios/farmacología , Isotiocianatos/uso terapéutico , Óxido Nítrico/antagonistas & inhibidores , Animales , Antiinflamatorios/química , Línea Celular , Ensayos de Selección de Medicamentos Antitumorales , Ratones , Óxido Nítrico/química , Óxido Nítrico/metabolismo , Preparaciones de Plantas/química , Solubilidad , Relación Estructura-Actividad , Wasabia/químicaRESUMEN
Serum-free mouse embryo (SFME) cells, the astrocyte progenitor cells in the central nervous system (CNS), were exposed to 10 ng/ml leukemia inhibitory factor (LIF) and 10 ng/ml bone morphogenic protein 2 (BMP2) to induce differentiation, and expression of cell-type specific markers. Nestin, a marker of early neural lineage, betaIII-tubulin, a marker of neuronal lineage, oligodendrocyte marker O4 (O4), a marker of oligodendrocytic lineage and glial fibrillary acidic protein (GFAP), a marker of astrocytic lineage, were analyzed. Characteristics of SFME cells, as a CNS progenitor, were identified and a possible mechanism, underlying SFME cell specification into an astrocytic lineage upon differentiation, was investigated. These markers were present, both at the initial proliferative phase and after induction of differentiation. GFAP expression increased strongly upon differentiation, while expression of the other markers changed very little. These results indicate that astrocytic differentiation is associated with the asymmetric production of these markers, rather than through induction of astrocytic markers.