[Study the role of lysosome-associated membrane protein type 2A for immune-mediated liver injury of primary biliary cholangitis].

Objective: To investigate the role of lysosome-associated membrane protein type 2A (LAMP-2A) for immune-mediated liver injury of primary biliary cholangitis (PBC). Methods: The association between LAMP-2A expression and PBC was examined by immunohistochemistry and electron microscopy in liver tissue samples from patients with PBC. Furthermore, the immunological damage of LAMP-2A overexpression on mouse liver was observed by adeno-associated virus (AAV) overexpression technique. The expression level of mRNA was analyzed by Student's t-test. The data were graphed and analyzed statistically using graphpad prism 5 (GraphPad Software).A value of p < 0.05 was considered statistically significant. Results: The expression of LAMP-2A in liver tissue of PBC patients was increased, and the autophagosome formation was observed in hepatocytes. C57BL/6 mice were injected into the caudal vein with LAMP-2A AAV for 6 weeks. The formation of autophagosomes in mouse hepatocytes was increased significantly. The expression of related molecules was abnormal; simultaneously, the degree of lymphocyte infiltration in the liver tissue of mice was significantly higher than the control group. Conclusion: An overexpression of LAMP-2A in the liver of patients with PBC may induce and/or promote the hepatic inflammatory response, especially the portal inflammatory infiltrate.

Western mental health training for traditional Chinese medicine practitioners.

OBJECTIVE: To investigate the impact of a Western mental health training course for Traditional Chinese Medicine (TCM) practitioners. METHOD: A combined qualitative and quantitative approach was applied to examine the changes in the TCM practitioners' clinical practice characteristics and attitudes. Focus groups and structured questionnaire surveys were conducted to compare their responses before and after the Course. RESULTS: After a 10-week training course conducted by psychiatrists and family physicians, there were significant changes in confidence of the TCM practitioners for diagnosis (33% being confident before the Course vs. 76% after the Course) and management (24% vs. 55%) of common mental health problems. The causal effects of better classifications to recognition of mental health problems were explained by the qualitative responses. Proportion of TCM practitioners being confident of referring mental health patients to other healthcare professionals doubled from 25% to 50% after the Course. Nonetheless, there was no significant change in percentage of these patients being recommended to Western doctors owing to a lack of formal referral channel. CONCLUSIONS: Western mental health training for TCM practitioners has positive impact on their clinical practice. However, the practical barriers in making referrals highlight the need of closer collaboration between conventional and traditional medicine.

Antibiotic use: do parents act differently for their children?

BACKGROUND: Many children in the community take antibiotics inappropriately. Previous studies comparing parents with other adults suggest that parents are more judicious with antibiotics for their children. This study aims to explore the difference between parents' use of antibiotics for themselves and for their children. METHODS: The study adopted a combined qualitative and quantitative approach. Eight focus groups were conducted with 56 participants purposively recruited from community centres and of different socio-economic strata. The qualitative data collected were used to construct a questionnaire for the telephone survey, which recruited 2471 adults randomly selected from the local household directory, of whom 547 had ever brought their children or grand children for medical consultation. RESULTS: Both the qualitative and quantitative approaches showed that parents were more cautious with antibiotics for children than for themselves. The main reason was their concern of side effects. Fever was the most important drive for their desire of antibiotics for children. The misconception of antibiotics' effectiveness for sore throat was another determinant of the desire. These attitudes and behaviour were not affected by the respondents' sex, age, education or household income. CONCLUSION: On the whole, parents did to their children what they would do for themselves, but to a lesser extent. Parents' better knowledge and attitudes will lead to more appropriate use of antibiotics for their children.