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1.
Intern Med J ; 54(3): 511-515, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38475863

RESUMO

Although reports of outbreaks of dengue-like diseases in the Asia Pacific region were frequent from about 1870, the disease probably did not become endemic in Australia until about 1885. Several seminal discoveries about this disease were made in Queensland and later in Sydney. These included a refined case definition for dengue, identification of the mosquito vector, demonstration of a viraemia and its duration, quantification of the incubation time, demonstration of immunity after experimental infection and recognition that the virus could cause a fatal haemorrhagic fever, and this was more frequent in second or subsequent infections. Australian research was foundational to the modern understanding of dengue.


Assuntos
Dengue , Animais , Humanos , Austrália/epidemiologia , Dengue/epidemiologia , Surtos de Doenças , Queensland/epidemiologia
2.
Emerg Infect Dis ; 26(12): 3061-3065, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33219791

RESUMO

During 2017-2018, Barmah Forest virus was recovered from mosquitoes trapped in military training areas in Australia and from a soldier infected at 1 of these areas. Phylogenies of the nucleotide sequences of the envelope glycoprotein gene E2 and the 3' untranslated region suggest that 2 lineages are circulating in eastern Australia.


Assuntos
Alphavirus , Arbovírus , Culicidae , Militares , Alphavirus/genética , Animais , Austrália/epidemiologia , Humanos
3.
PLoS Comput Biol ; 15(11): e1006668, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31710599

RESUMO

The titre of virus in a dengue patient and the duration of this viraemia has a profound effect on whether or not a mosquito will become infected when it feeds on the patient and this, in turn, is a key driver of the magnitude of a dengue outbreak. The assessment of the heterogeneity of viral dynamics in dengue-infected patients and its precise treatment are still uncertain. Infection onset, patient physiology and immune response are thought to play major roles in the development of the viral load. Research has explored the interference and spontaneous generation of defective virus particles, but have not examined both the antibody and defective particles during natural infection. We explore the intrinsic variability in the within-host dynamics of viraemias for a population of patients using the method of population of models (POMs). A dataset from 208 patients is used to initially calibrate 20,000 models for the infection kinetics for each of the four dengue virus serotypes. The calibrated POMs suggests that naturally generated defective particles may interfere with the viraemia, but the generated defective virus particles are not adequate to reduce high fever and viraemia duration. The effect of adding excess defective dengue virus interfering particles to patients as a therapeutic is evaluated using the calibrated POMs in a bang-bang (on-off or two-step) optimal control setting. Bang-bang control is a class of binary feedback control that turns either 'ON' or 'OFF' at different time points, determined by the system feedback. Here, the bang-bang control estimates the mathematically optimal dose and duration of the intervention for each model in the POM set.


Assuntos
Vírus da Dengue/fisiologia , Dengue/virologia , Interações entre Hospedeiro e Microrganismos/fisiologia , Animais , Culicidae , Vírus Defeituosos , Humanos , Modelos Teóricos , Carga Viral/fisiologia , Viremia , Vírion , Replicação Viral
4.
Emerg Infect Dis ; 25(10): 1793-1801, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31538560

RESUMO

Two outbreaks of epidemic polyarthritis occurred among Australian Defence Force personnel during and following short military exercises in the Shoalwater Bay Training Area, northeastern Australia, in 2016 and 2017. Ross River virus (RRV) IgM was detected in acute-phase serum samples from most patients (28/28 in 2016 and 25/31 in 2017), and RRV was recovered from 4/38 serum samples assayed (1/21 in 2016 and 3/17 in 2017). Phylogenetic analyses of RRV envelope glycoprotein E2 and nonstructural protein nsP3 nucleotide sequences segregated the RRV isolates obtained in 2016 and 2017 outbreaks into 2 distinct sublineages, suggesting that each outbreak was caused by a different strain of RRV. The spatiotemporal characteristics of the 2016 outbreak suggested that some of the infections involved human-mosquito-human transmission without any intermediate host. These outbreaks highlight the importance of personal protective measures in preventing vectorborne diseases for which no vaccine or specific prophylaxis exists.


Assuntos
Infecções por Alphavirus/epidemiologia , Artrite/epidemiologia , Epidemias , Militares , Ross River virus , Adulto , Infecções por Alphavirus/virologia , Artrite/virologia , Epidemias/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Militares/estatística & dados numéricos , Filogenia , Queensland/epidemiologia , Ross River virus/genética , Adulto Jovem
5.
Emerg Infect Dis ; 25(4): 827-830, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30882332

RESUMO

A unique outbreak of Ross River virus (RRV) infection was reported in Fiji in 1979. In 2013, RRV seroprevalence among residents was 46.5% (362/778). Of the residents who were seronegative in 2013 and retested in 2015, 10.9% (21/192) had seroconverted to RRV, suggesting ongoing endemic circulation of RRV in Fiji.


Assuntos
Infecções por Alphavirus/diagnóstico , Ross River virus/imunologia , Infecções por Alphavirus/sangue , Infecções por Alphavirus/epidemiologia , Anticorpos Antivirais/sangue , Fiji/epidemiologia , Humanos , Ross River virus/isolamento & purificação , Estudos Soroepidemiológicos
6.
7.
Emerg Infect Dis ; 22(12): 2146-2148, 2016 12.
Artigo em Inglês | MEDLINE | ID: mdl-27869598

RESUMO

Dengue virus type 2 was isolated from a tourist who returned from Borneo to Australia. Phylogenetic analysis identified this virus as highly divergent and occupying a basal phylogenetic position relative to all known human and sylvatic dengue virus type 2 strains and the most divergent lineage not assigned to a new serotype.


Assuntos
Vírus da Dengue/classificação , Vírus da Dengue/genética , Dengue/epidemiologia , Dengue/virologia , Viagem , Austrália/epidemiologia , Bornéu/epidemiologia , Dengue/transmissão , Evolução Molecular , Variação Genética , Genoma Viral , Genótipo , Humanos , Filogenia , RNA Viral , Sorogrupo
8.
PLoS Pathog ; 9(2): e1003193, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23468631

RESUMO

Intra-host sequence data from RNA viruses have revealed the ubiquity of defective viruses in natural viral populations, sometimes at surprisingly high frequency. Although defective viruses have long been known to laboratory virologists, their relevance in clinical and epidemiological settings has not been established. The discovery of long-term transmission of a defective lineage of dengue virus type 1 (DENV-1) in Myanmar, first seen in 2001, raised important questions about the emergence of transmissible defective viruses and their role in viral epidemiology. By combining phylogenetic analyses and dynamical modeling, we investigate how evolutionary and ecological processes at the intra-host and inter-host scales shaped the emergence and spread of the defective DENV-1 lineage. We show that this lineage of defective viruses emerged between June 1998 and February 2001, and that the defective virus was transmitted primarily through co-transmission with the functional virus to uninfected individuals. We provide evidence that, surprisingly, this co-transmission route has a higher transmission potential than transmission of functional dengue viruses alone. Consequently, we predict that the defective lineage should increase overall incidence of dengue infection, which could account for the historically high dengue incidence reported in Myanmar in 2001-2002. Our results show the unappreciated potential for defective viruses to impact the epidemiology of human pathogens, possibly by modifying the virulence-transmissibility trade-off, or to emerge as circulating infections in their own right. They also demonstrate that interactions between viral variants, such as complementation, can open new pathways to viral emergence.


Assuntos
Vírus Defeituosos/fisiologia , Vírus da Dengue/fisiologia , Dengue/virologia , Surtos de Doenças , Interações Hospedeiro-Patógeno , Dengue/epidemiologia , Dengue/transmissão , Doenças Endêmicas , Humanos , Epidemiologia Molecular , Mianmar/epidemiologia , Filogeografia , Especificidade da Espécie
9.
Emerg Infect Dis ; 20(6): 1034-6, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24856252

RESUMO

After an 18-year absence, dengue virus serotype 3 reemerged in the South Pacific Islands in 2013. Outbreaks in western (Solomon Islands) and eastern (French Polynesia) regions were caused by different genotypes. This finding suggested that immunity against dengue virus serotype, rather than virus genotype, was the principal determinant of reemergence.


Assuntos
Vírus da Dengue/genética , Dengue/epidemiologia , Surtos de Doenças , Proteínas Virais/genética , Aedes/virologia , Animais , Dengue/transmissão , Dengue/virologia , Vírus da Dengue/isolamento & purificação , Vetores de Doenças , Genótipo , Humanos , Melanesia/epidemiologia , Polinésia/epidemiologia , Sorogrupo
10.
Virol J ; 11: 61, 2014 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-24684835

RESUMO

BACKGROUND: The epidemiology of dengue in the South Pacific has been characterized by transmission of a single dominant serotype for 3-5 years, with subsequent replacement by another serotype. From 2001 to 2008 only DENV-1 was reported in the Pacific. In 2008, DENV-4 emerged and quickly displaced DENV-1 in the Pacific, except in New Caledonia (NC) where DENV-1 and DENV-4 co-circulated in 2008-2009. During 2012-2013, another DENV-1 outbreak occurred in NC, the third DENV-1 outbreak in a decade. Given that dengue is a serotype-specific immunizing infection, the recurrent outbreaks of a single serotype within a 10-year period was unexpected. FINDINGS: This study aimed to inform this phenomenon by examining the phylogenetic characteristics of the DENV-1 viruses in NC and other Pacific islands between 2001 and 2013. As a result, we have demonstrated that NC experienced introductions of viruses from both the Pacific (genotype IV) and South-east Asia (genotype I). Moreover, whereas genotype IV and I were co-circulating at the beginning of 2012, we observed that from the second half of 2012, i.e. during the major DENV-1 outbreak, all analyzed viruses were genotype I suggesting that a genotype switch occurred. CONCLUSIONS: Repeated outbreaks of the same dengue serotype, as observed in NC, is uncommon in the Pacific islands. Why the earlier DENV-1 outbreaks did not induce sufficient herd immunity is unclear, and likely multifactorial, but the robust vector control program may have played a role by limiting transmission and thus maintaining a large susceptible pool in the population.


Assuntos
Vírus da Dengue/classificação , Vírus da Dengue/genética , Dengue/epidemiologia , Dengue/virologia , Análise por Conglomerados , Vírus da Dengue/isolamento & purificação , Humanos , Epidemiologia Molecular , Dados de Sequência Molecular , Nova Caledônia/epidemiologia , Filogenia , RNA Viral/genética , Análise de Sequência de DNA
11.
Transfus Apher Sci ; 50(1): 59-62, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24332363

RESUMO

Risk of dengue virus in the blood supply has been demonstrated in recent studies. In this paper, Chabahar in Sistan and Baluchestan province, south east of Iran, was selected for studying dengue infection because of its climatic and geographical situation in the middle of the transit way between East Asia and other countries. The blood samples were taken from volunteer healthy donors who were referred to the Chabahar Blood Center for blood donation. The presence of dengue virus (DENV) was studied by detecting IgG to DENV by enzyme linked immune sorbent assay (ELISA). Reactive ELISA results were confirmed by an immune flouorescence assay (IFA). According to the results, some of the healthy donors were infected by DENV, which could not been recognized in donor selection. Therefore, special attention should be paid to the criteria of donor selection and additional screening tests are recommended.


Assuntos
Doadores de Sangue , Dengue/epidemiologia , Dengue/virologia , Dengue/sangue , Vírus da Dengue , Ensaio de Imunoadsorção Enzimática , Feminino , Imunofluorescência , Geografia , Voluntários Saudáveis , Humanos , Imunoglobulina G/sangue , Irã (Geográfico)/epidemiologia , Masculino
12.
Am J Reprod Immunol ; 89(3): e13668, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36484330

RESUMO

PROBLEM: HSV-2 infected more than 491 million people aged 15-49 world-wide in 2016. The morbidity associated with recurrent infections and the increased risk of HIV infection make this a major health problem. To date there is no effective vaccine. Because HSV-2 ascends to the dorsal route ganglion within 12-18 h of infection, an effective vaccine will need to elicit a strong local resident CD8+ T cell response to prevent the infection from becoming life-long. METHOD OF STUDY: Using a mouse model we investigated the potential of oral immunization with a novel lipid adjuvant (LiporaleTM ) followed by local vaginal application of an inflammatory agents to protect against primary HSV-2 infections. RESULTS: Oral vaccination of mice with live-attenuated HSV-2 in Liporale followed by vaginal application of DNFB or CXCL9/10 led to recruitment of tissue-resident CD8+ memory cells into the genital epithelia. This prime and pull vaccination strategy provided complete protection against wild-type HSV-2 challenge and prevented viral dissemination to the spinal cords. CONCLUSIONS: Activation of mucosal immunity by oral immunization, combined with induction of transient local genital inflammation can recruit long-lived tissue resident CD8+ T cells into the genital epithelium, providing significant protection against primary HSV-2 infection.


Assuntos
Infecções por HIV , Herpes Genital , Feminino , Humanos , Herpesvirus Humano 2 , Linfócitos T CD8-Positivos , Herpes Genital/prevenção & controle , Vagina , Vacinação
13.
J Virol ; 85(11): 5674-8, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21430052

RESUMO

We examined the structure and extent of genetic diversity in intrahost populations of Ross River virus (RRV) in samples from six human patients, focusing on the nonstructural (nsP3) and structural (E2) protein genes. Strikingly, although the samples were collected from contrasting ecological settings 3,000 kilometers apart in Australia, we observed multiple viral lineages in four of the six individuals, which is indicative of widespread mixed infections. In addition, a comparison with previously published RRV sequences revealed that these distinct lineages have been in circulation for at least 5 years, and we were able to document their long-term persistence over extensive geographical distances.


Assuntos
Infecções por Alphavirus/virologia , Variação Genética , Ross River virus/classificação , Ross River virus/genética , Austrália , Análise por Conglomerados , Genótipo , Humanos , Ross River virus/isolamento & purificação , Análise de Sequência de DNA , Proteínas não Estruturais Virais/genética , Proteínas Estruturais Virais/genética
14.
Microbiol Resour Announc ; 10(46): e0083821, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34792379

RESUMO

Ross River virus recovered from a South Australian patient during an outbreak of epidemic polyarthritis in 1971 is the earliest known genome sequence with the duplicated 12-amino-acid motif in the nsP3 protein that was found in strains responsible for the outbreak of epidemic polyarthritis in the Pacific region from 1979 to 1980.

15.
Commun Biol ; 4(1): 557, 2021 05 11.
Artigo em Inglês | MEDLINE | ID: mdl-33976375

RESUMO

Dengue virus (DENV) is spread from human to human through the bite of the female Aedes aegypti mosquito and leads to about 100 million clinical infections yearly. Treatment options and vaccine availability for DENV are limited. Defective interfering particles (DIPs) are considered a promising antiviral approach but infectious virus contamination has limited their development. Here, a DENV-derived DIP production cell line was developed that continuously produced DENV-free DIPs. The DIPs contained and could deliver to cells a DENV serotype 2 subgenomic defective-interfering RNA, which was originally discovered in DENV infected patients. The DIPs released into cell culture supernatant were purified and could potently inhibit replication of all DENV serotypes in cells. Antiviral therapeutics are limited for many viral infection. The DIP system described could be re-purposed to make antiviral DIPs for many other RNA viruses such as SARS-CoV-2, yellow fever, West Nile and Zika viruses.


Assuntos
Vírus Defeituosos , Vacinas contra Dengue/uso terapêutico , Vírus da Dengue/crescimento & desenvolvimento , Dengue/prevenção & controle , Replicação Viral , Animais , Linhagem Celular Tumoral , Chlorocebus aethiops , Vírus Defeituosos/genética , Vírus Defeituosos/metabolismo , Dengue/virologia , Vírus da Dengue/genética , Vírus da Dengue/metabolismo , Genes Reporter , Células HEK293 , Interações Hospedeiro-Patógeno , Humanos , Proteínas Luminescentes/biossíntese , Proteínas Luminescentes/genética , RNA Viral/biossíntese , RNA Viral/genética , Células Vero , Carga Viral
16.
Emerg Infect Dis ; 16(1): 123-5, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20031057

RESUMO

Since 2000-2001, dengue virus type 1 has circulated in the Pacific region. However, in 2007, type 4 reemerged and has almost completely displaced the strains of type 1. If only 1 serotype circulates at any time and is replaced approximately every 5 years, DENV-3 may reappear in 2012.


Assuntos
Vírus da Dengue , Dengue/epidemiologia , Dengue/virologia , Vírus da Dengue/genética , Humanos , Ilhas do Pacífico/epidemiologia , Filogenia , Reação em Cadeia da Polimerase , Proteínas do Envelope Viral/genética
17.
J Gen Virol ; 91(Pt 1): 182-8, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19759236

RESUMO

Ross River virus (RRV) is a mosquito-borne member of the genus Alphavirus that causes epidemic polyarthritis in humans, costing the Australian health system at least US$10 million annually. Recent progress in RRV vaccine development requires accurate assessment of RRV genetic diversity and evolution, particularly as they may affect the utility of future vaccination. In this study, we provide novel RRV genome sequences and investigate the evolutionary dynamics of RRV from time-structured E2 gene datasets. Our analysis indicates that, although RRV evolves at a similar rate to other alphaviruses (mean evolutionary rate of approx. 8x10(-4) nucleotide substitutions per site year(-1)), the relative genetic diversity of RRV has been continuously low through time, possibly as a result of purifying selection imposed by replication in a wide range of natural host and vector species. Together, these findings suggest that vaccination against RRV is unlikely to result in the rapid antigenic evolution that could compromise the future efficacy of current RRV vaccines.


Assuntos
Infecções por Alphavirus/virologia , Evolução Molecular , Variação Genética , Ross River virus/classificação , Ross River virus/genética , Vacinas Virais/imunologia , Infecções por Alphavirus/epidemiologia , Infecções por Alphavirus/prevenção & controle , Animais , Austrália/epidemiologia , Proteínas do Capsídeo/genética , Análise por Conglomerados , Culicidae , Vetores de Doenças , Genoma Viral , Humanos , Dados de Sequência Molecular , Filogenia , RNA Viral/genética , Ross River virus/isolamento & purificação , Análise de Sequência de DNA , Homologia de Sequência , Proteínas do Envelope Viral/genética
18.
Hum Vaccin ; 6(11): 906-14, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20864814

RESUMO

A randomized, double-blind, study was conducted to evaluate the safety, tolerability and immunogenicity of a live attenuated Japanese encephalitis chimeric virus vaccine (JE-CV) co-administered with live attenuated yellow fever vaccine (YF-17D strain; Stamaril®, Sanofi Pasteur) or administered successively. Participants (n = 108) were randomized to receive: YF followed by JE-CV 30 days later, JE followed by YF 30 days later, or the co-administration of JE and YF followed or preceded by placebo 30 days later or earlier. Placebo was used in a double-dummy fashion to ensure masking. Neutralizing antibody titers against JE-CV, YF-17D and selected wild-type JE strains was determined using a 50% serum-dilution plaque reduction neutralization test. Seroconversion was defined as the appearance of a neutralizing antibody titer above the assay cut-off post-immunization when not present pre-injection at day 0, or a least a four-fold rise in neutralizing antibody titer measured before the pre-injection day 0 and later post vaccination samples. There were no serious adverse events. Most adverse events (AEs) after JE vaccination were mild to moderate in intensity, and similar to those reported following YF vaccination. Seroconversion to JE-CV was 100% and 91% in the JE/YF and YF/JE sequential vaccination groups, respectively, compared with 96% in the co-administration group. All participants seroconverted to YF vaccine and retained neutralizing titers above the assay cut-off at month six. Neutralizing antibodies against JE vaccine were detected in 82-100% of participants at month six. These results suggest that both vaccines may be successfully co-administered simultaneously or 30 days apart.


Assuntos
Encefalite Japonesa/prevenção & controle , Vacinas contra Encefalite Japonesa/administração & dosagem , Vacinas contra Encefalite Japonesa/imunologia , Vacinação/métodos , Vacina contra Febre Amarela/administração & dosagem , Vacina contra Febre Amarela/imunologia , Febre Amarela/prevenção & controle , Adolescente , Adulto , Anticorpos Neutralizantes , Anticorpos Antivirais/sangue , Método Duplo-Cego , Feminino , Humanos , Vacinas contra Encefalite Japonesa/efeitos adversos , Masculino , Pessoa de Meia-Idade , Testes de Neutralização , Placebos/administração & dosagem , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/efeitos adversos , Vacinas Atenuadas/imunologia , Vacinas Combinadas/administração & dosagem , Vacinas Combinadas/efeitos adversos , Vacinas Combinadas/imunologia , Ensaio de Placa Viral , Vacina contra Febre Amarela/efeitos adversos , Adulto Jovem
19.
Hum Vaccin ; 6(12): 1038-46, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21150279

RESUMO

In a randomized, double-blind study, 202 healthy adults were randomized to receive a live, attenuated Japanese encephalitis chimeric virus vaccine (JE-CV) and placebo 28 days apart in a cross-over design. A subgroup of 98 volunteers received a JE-CV booster at month 6. Safety, immunogenicity, and persistence of antibodies to month 60 were evaluated. There were no unexpected adverse events (AEs) and the incidence of AEs between JE-CV and placebo were similar. There were three serious adverse events (SAE) and no deaths. A moderately severe case of acute viral illness commencing 39 days after placebo administration was the only SAE considered possibly related to immunization. 99% of vaccine recipients achieved a seroprotective antibody titer ≥ 10 to JE-CV 28 days following the single dose of JE-CV, and 97% were seroprotected at month 6. Kaplan Meier analysis showed that after a single dose of JE-CV, 87% of the participants who were seroprotected at month 6 were still protected at month 60. This rate was 96% among those who received a booster immunization at month 6. 95% of subjects developed a neutralizing titer ≥ 10 against at least three of the four strains of a panel of wild-type Japanese encephalitis virus (JEV) strains on day 28 after immunization. At month 60, that proportion was 65% for participants who received a single dose of JE-CV and 75% for the booster group. These results suggest that JE-CV is safe, well tolerated and that a single dose provides long-lasting immunity to wild-type strains.


Assuntos
Vacinas contra Encefalite Japonesa/imunologia , Adolescente , Adulto , Anticorpos Antivirais/sangue , Estudos Cross-Over , Método Duplo-Cego , Encefalite Japonesa/prevenção & controle , Feminino , Experimentação Humana , Humanos , Imunização Secundária/métodos , Vacinas contra Encefalite Japonesa/administração & dosagem , Vacinas contra Encefalite Japonesa/efeitos adversos , Masculino , Pessoa de Meia-Idade , Placebos/administração & dosagem , Fatores de Tempo , Vacinação/métodos , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/efeitos adversos , Vacinas Atenuadas/imunologia , Adulto Jovem
20.
Viruses ; 12(5)2020 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-32443524

RESUMO

The host-vector shuttle and the bottleneck in dengue transmission is a significant aspect with regard to the study of dengue outbreaks. As mosquitoes require 100-1000 times more virus to become infected than human, the transmission of dengue virus from human to mosquito is a vulnerability that can be targeted to improve disease control. In order to capture the heterogeneity in the infectiousness of an infected patient population towards the mosquito population, we calibrate a population of host-to-vector virus transmission models based on an experimentally quantified infected fraction of a mosquito population. Once the population of models is well-calibrated, we deploy a population of controls that helps to inhibit the human-to-mosquito transmission of the dengue virus indirectly by reducing the viral load in the patient body fluid. We use an optimal bang-bang control on the administration of the defective virus (transmissible interfering particles (TIPs)) to symptomatic patients in the course of their febrile period and observe the dynamics in successful reduction of dengue spread into mosquitoes.


Assuntos
Vírus Defeituosos/fisiologia , Vírus da Dengue/fisiologia , Dengue/prevenção & controle , Dengue/transmissão , Mosquitos Vetores/virologia , Aedes/virologia , Animais , Dengue/virologia , Humanos , Modelos Teóricos , Carga Viral , Viremia/virologia
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