Multivariate approach in popcorn genotypes using the Ward-MLM strategy: morpho-agronomic analysis and incidence of Fusarium spp.

The multivariate analyses are useful tools to estimate the genetic variability between accessions. In the breeding programs, the Ward-Modified Location Model (MLM) multivariate method has been a powerful strategy to quantify variability using quantitative and qualitative variables simultaneously. The present study was proposed in view of the dearth of information about popcorn breeding programs under a multivariate approach using the Ward-MLM methodology. The objective of this study was thus to estimate the genetic diversity among 37 genotypes of popcorn aiming to identify divergent groups associated with morpho-agronomic traits and traits related to resistance to Fusarium spp. To this end, 7 qualitative and 17 quantitative variables were analyzed. The experiment was conducted in 2014, at Universidade Estadual do Norte Fluminense, located in Campos dos Goytacazes, RJ, Brazil. The Ward-MLM strategy allowed the identification of four groups as follows: Group I with 10 genotypes, Group II with 11 genotypes, Group III with 9 genotypes, and Group IV with 7 genotypes. Group IV was distant in relation to the other groups, while groups I, II, and III were near. The crosses between genotypes from the other groups with those of group IV allow an exploitation of heterosis. The Ward-MLM strategy provided an appropriate grouping of genotypes; ear weight, ear diameter, and grain yield were the traits that most contributed to the analysis of genetic diversity.

Genetic control and combining ability of agronomic attributes and northern leaf blight-related attributes in popcorn.

The present study was conducted to investigate the genetic control and to estimate the general and specific combining abilities of popcorn for agronomic attributes and attributes related to resistance to northern leaf blight (NLB). The 56 hybrids (F1 and reciprocals), together with the eight parent lines and six controls, were evaluated in two harvests, in a randomized-block design with four replications. Dominance components were more expressive than the additive components for grain yield and expression of resistance, and hybridization was the most suitable option for obtaining resistant and productive genotypes. For grain yield, popping expansion, and resistance to NLB, there was no significance for reciprocal effects, which indicates that the direction in which the cross is performed does not interfere with the hybrid's performance. Then, the superior hybrids recommended for more profitable growth were P8 x L61, L61 x L76, and L61 x L77.

Stability of annual biomass energy production of elephant grass (Pennisetum purpureum Schum.) genotypes in the Northern region of the Rio de Janeiro State, Brazil.

The objectives of this study were to estimate the stability of dry biomass production of elephant grass genotypes under an annual harvest regime, in soil-climatic conditions of the Northern region of the Rio de Janeiro State, Brazil, and to compare methodologies for stability analyses of Yates and Cochran (YC), Plaisted and Peterson (PP), Annicchiarico (ANN), Lin and Binns (LB), Huenh (HU), and Kang and Phan (KP). A randomized block design with 83 treatments and two replicates was adopted. Four annual harvests were performed (2012-2015) and dry matter yield (DMY, t.ha-1.year-1) was evaluated. Individual and combined analyses of variance for DMY revealed significant effects for genotypes, harvests, and for the genotype x harvest interaction at the probability levels of 1 and 5%. Genotypes indicated by the YC and PP methods are associated with higher stability and lower DMY. The weighting of KP with YC and PP was highly effective in associating stability with DMY. The LB and ANN methods showed strong agreement with each other and produced similar classifications as to phenotypic stability, and so we recommend using one or the other. Genotypes Elefante Cachoeiro do Itapemirim, Cuba-116, Taiwan A-46, P241 Piracicaba, Taiwan A-144, Cameroon - Piracicaba, 10 AD IRI, Guaçu/I,Z,2, Mineirão IPEACO, Taiwan A-121, IJ7125 cv EMPASC308, 903-77, Mole de Volta Grande, and Porto Rico 534-B showed high stability and DMY, standing out as promising genotypes for the soil-climatic conditions of the Northern region of the Rio de Janeiro State. The methodologies based on ANOVA and non-parametric analyses were complementary and increased reliability in the recommendation of genotypes.

Indices estimated using REML/BLUP and introduction of a super-trait for the selection of progenies in popcorn.

Selection indices commonly utilize economic weights, which become arbitrary genetic gains. In popcorn, this is even more evident due to the negative correlation between the main characteristics of economic importance - grain yield and popping expansion. As an option in the use of classical biometrics as a selection index, the optimal procedure restricted maximum likelihood/best linear unbiased predictor (REML/BLUP) allows the simultaneous estimation of genetic parameters and the prediction of genotypic values. Based on the mixed model methodology, the objective of this study was to investigate the comparative efficiency of eight selection indices estimated by REML/BLUP for the effective selection of superior popcorn families in the eighth intrapopulation recurrent selection cycle. We also investigated the efficiency of the inclusion of the variable "expanded popcorn volume per hectare" in the most advantageous selection of superior progenies. In total, 200 full-sib families were evaluated in two different areas in the North and Northwest regions of the State of Rio de Janeiro, Brazil. The REML/BLUP procedure resulted in higher estimated gains than those obtained with classical biometric selection index methodologies and should be incorporated into the selection of progenies. The following indices resulted in higher gains in the characteristics of greatest economic importance: the classical selection index/values attributed by trial, via REML/BLUP, and the greatest genotypic values/expanded popcorn volume per hectare, via REML. The expanded popcorn volume per hectare characteristic enabled satisfactory gains in grain yield and popping expansion; this characteristic should be considered super-trait in popcorn breeding programs.

Selection index based on the relative importance of traits and possibilities in breeding popcorn.

One of the major difficulties faced by popcorn breeders is the negative correlation between popping expansion (PE) and grain yield (GY). It is necessary to overcome this difficulty to obtain promising genotypes. One helpful tool in this process is a selection index because it allows multiple features of interest to be selected. Thus, the present study proposes a new and comprehensive selection index applied in 169 half-sib families in UEM-Co1 and UEM-Co2 composites during two cycles of recurrent selection. An experiment was conducted in a 13 x 13 lattice square in the 2004/2005 and 2006/2007 crop years in Maringá, Paraná State, and PE and GY were evaluated. To calculate Fi statistics, the following relative importance (RI) assignments were used: 0.5 for both PE and GY, and 0.70 and 0.30 for PE and GY, respectively. Families were classified according to Fi values such that Fi = 0 indicated that genotypes met the average of those selected by direct selection, Fi < 0 indicated that genotypes fell below the average of those selected, and Fi > 0 indicated that genotypes exceeded the average of those selected. Thus, desirable values of Fi were positive, indicating that the selected families were higher than those families that would be selected by direct selection for both traits. Therefore, we concluded that the novel Fi statistic was satisfactory for family selection because simultaneous and higher gains for both traits in both composites were obtained.

Biometric analysis of arabica coffee grown in low potassium nutrient solution under greenhouse conditions.

Genetic parameters and associations between morpho-agronomic traits and nutritional efficiencies of arabica coffee cultivars were estimated to identify promising traits to assist in the selection of coffee genotypes efficient in potassium use, under limiting conditions of this nutrient. The experiment was conducted in a greenhouse with 20 arabica coffee cultivars grown in nutrient solution with a low potassium level (1.5 mM), using a randomized block design with three replicates. The traits evaluated were plant height, number of leaves, number of nodes, internode length, stem diameter, leaf area, rooting efficiency, potassium absorption efficiency, potassium translocation efficiency, biomass production efficiency, and potassium use efficiency. Genetic variability among coffee cultivars for all the evaluated traits was found. The phenotypic variance for all traits showed a higher contribution of genetic variance compared to environmental variance. Plant height, internode length, stem diameter, leaf area, biomass production efficiency, and potassium use efficiency had a genotypic determination coefficient (H2) above 80% and variation index greater than one. Leaf area and stem diameter had significant and positive genetic correlations with rooting, biomass production, and potassium use efficiencies. Stem diameter has great potential for use in breeding programs with a goal of indirect selection of cultivars that have greater potassium use efficiency in environments with restrictions of this nutrient.

Combining ability of S3 progenies for key agronomic traits in popcorn: comparison of testers in top-crosses.

The successful development of hybrid cultivars depends on the reliability of estimated combining ability of the parent lines. The objectives of this study were to assess the combining ability of partially inbred S3 families of popcorn derived from the open-pollinated variety UENF 14, via top-crosses with four testers, and to compare the testers for their ability to discriminate the S3 progenies. The experiment was conducted in the 2015/2016 crop season, in an incomplete-block (Lattice) design with three replications. The following agronomic traits were evaluated: average plant height, grain yield (GY), popping expansion (PE), and expanded popcorn volume per hectare. The top-cross hybrid, originating from the BRS-Angela vs S3 progeny 10 combination, was indicated as promising, showing high values for specific combining ability for GY and PE. For the S3 progenies that showed high and positive GCA values for GY and PE, the continuity of the breeding program is recommended, with the advance of self-pollination generations. Fasoulas' differentiation index discriminated the BRS-Angela tester as the most suitable for identifying the superior progenies.

Proposal of a super trait for the optimum selection of popcorn progenies based on path analysis.

A challenge faced by popcorn breeding programs is the existence of a negative correlation between the two main traits, popping expansion and yield, which hinders simultaneous gains. The objective of this study was to investigate the use of a new variable or super trait, which favors the reliable selection of superior progenies. The super trait 'expanded popcorn volume per hectare' was introduced in the evaluation of 200 full-sib families of the eighth recurrent intrapopulation selection cycle, which were arranged in randomized blocks with three replicates in two environments. Although the inability to obtain simultaneous gains through selection via popping expansion or yield was confirmed, the super trait was positively associated with both yield and popping expansion, allowing simultaneous gains via indirect selection using 'expanded popcorn volume per hectare' as the main trait. This approach is recommended because this super trait can be used in breeding programs to optimize selective gains for the crop.

Genetic gains in the UENF-14 popcorn population with recurrent selection.

The popcorn breeding program of Universidade Estadual do Norte Fluminense Darcy Ribeiro aims to provide farmers a cultivar with desirable agronomic traits, particularly with respect to grain yield (GY) and popping expansion (PE). We evaluated full-sib families from the seventh cycle of recurrent selection and estimated the genetic progress with respect to GY and PE. Eight traits were evaluated in 200 full-sib families that were randomized into blocks with two replicates per set in two contrasting environments, Campos dos Goytacazes and Itaocara, located in north and northwest Rio de Janeiro State, respectively. There were significant differences between sets in families with respect to all traits evaluated, which indicates genetic variability that may be explored in future cycles. Using random economic weights in the selection of superior progenies, the Mulamba and Mock index showed gains for PE and GY of 5.11 and 7.78%, respectively. Significant PE and GY increases were found when comparing the evolution of mean values of these two parameters that were assessed at cycles C0-C6 and predicted for C7. Thus, an advanced-cycle popcorn cultivar with genotypic superiority for the main traits of economic interest can be made available to farmers in Rio de Janeiro State.

Prospecting of popcorn hybrids for resistance to fall armyworm.

The fall armyworm, Spodoptera frugiperda, is the pest that causes the greatest economic losses for both common corn and popcorn crops, and the use of resistant plant genotypes is an important tool for integrated pest management. The goal of the present study was to evaluate the damage caused by S. frugiperda on single-cross popcorn hybrids under field conditions with natural infestation as well as to study the effect of 11 popcorn hybrids on the S. frugiperda life cycle under laboratory conditions. A completely randomized block design with 4 replicates was used for the field experiment, and a completely randomized design with 10 replicates was used for the laboratory experiment. In the field experiment, the damage caused by fall armyworm, grain yield, and popping expansion were quantified, and a diallel analysis was performed to select the best hybrids. For the laboratory experiment, caterpillars were obtained from laboratory cultures kept on an artificial diet and were fed with leaves from the 11 hybrids. Hybrids P7.0 x P9.4, P7.1 x P9.6, P7.2.0 x P9.3, P7.4.0 x P9.1 and P7.4.1 x P9.4 exhibited negative specific combining ability for injury by fall armyworm and positive specific combining ability for yield and popping expansion. In the laboratory experiment, the hybrids influenced the mean larval stage duration, mean larval mass, final larval mass, pupal stage duration, mean pupal mass, and adult longevity.

Genetically modified crops: Brazilian law and overview.

In Brazil, the first genetically modified (GM) crop was released in 1998, and it is estimated that 84, 78, and 50% of crop areas containing soybean, corn, and cotton, respectively, were transgenic in 2012. This intense and rapid adoption rate confirms that the choice to use technology has been the main factor in developing national agriculture. Thus, this review focuses on understanding these dynamics in the context of farmers, trade relations, and legislation. To accomplish this goal, a survey was conducted using the database of the National Cultivar Registry and the National Service for Plant Variety Protection of the Ministry of Agriculture, Livestock and Supply [Ministério da Agricultura, Pecuária e Abastecimento (MAPA)] between 1998 and October 13, 2013. To date, 36 events have been released: five for soybeans, 18 for corn, 12 for cotton, and one for beans. From these events, 1395 cultivars have been developed and registered: 582 for soybean, 783 for corn and 30 for cotton. Monsanto owns 73.05% of the technologies used to develop these cultivars, while the Dow AgroScience - DuPont partnership and Syngenta have 16.34 and 4.37% ownership, respectively. Thus, the provision of transgenic seeds by these companies is an oligopoly supported by legislation. Moreover, there has been a rapid replacement of conventional crops by GM crops, whose technologies belong almost exclusively to four multinational companies, with the major ownership by Monsanto. These results reflect a warning to the government of the increased dependence on multinational corporations for key agricultural commodities.

Experimental optimization of the number of blocks by means of algorithms parameterized by confidence interval in popcorn breeding.

The objective of this study was to determine the optimal number of repetitions to be used in competition trials of popcorn traits related to production and quality, including grain yield and expansion capacity. The experiments were conducted in 3 environments representative of the north and northwest regions of the State of Rio de Janeiro with 10 Brazilian genotypes of popcorn, consisting by 4 commercial hybrids (IAC 112, IAC 125, Zélia, and Jade), 4 improved varieties (BRS Ângela, UFVM-2 Barão de Viçosa, Beija-flor, and Viçosa) and 2 experimental populations (UNB2U-C3 and UNB2U-C4). The experimental design utilized was a randomized complete block design with 7 repetitions. The Bootstrap method was employed to obtain samples of all of the possible combinations within the 7 blocks. Subsequently, the confidence intervals of the parameters of interest were calculated for all simulated data sets. The optimal number of repetition for all of the traits was considered when all of the estimates of the parameters in question were encountered within the confidence interval. The estimates of the number of repetitions varied according to the parameter estimated, variable evaluated, and environment cultivated, ranging from 2 to 7. It is believed that only the expansion capacity traits in the Colégio Agrícola environment (for residual variance and coefficient of variation), and number of ears per plot, in the Itaocara environment (for coefficient of variation) needed 7 repetitions to fall within the confidence interval. Thus, for the 3 studies conducted, we can conclude that 6 repetitions are optimal for obtaining high experimental precision.

Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum.

Pathogenesis-related proteins (PRs) are among the defense mechanisms of plants that work as an important barrier to the development of pathogens. These proteins are classified into 17 families according to their amino acid sequences, serology, and/or biological or enzyme activity. The present study aimed to identify PRs associated with the pathosystem of Capsicum baccatum var. pendulum: Pepper yellow mosaic virus (PepYMV). Forty-five-day-old plants from accession UENF 1624, previously identified as resistant to PepYMV, were inoculated with the virus. Control and infected leaves were collected for analysis after 24, 48, 72, and 96 h. The inoculated and control plants were grown in cages covered with anti-aphid screens. Proteins were extracted from leaf tissue and the presence of ß-1,3-glucanase, chitinase, peroxidase, and lipid transport protein was verified. No difference was observed between the protein pattern of control and infected plants when ß-1,3-glucanase, chitinase, and lipid transport protein were compared. However, increased peroxidase expression was observed in infected plants at 48 and 72 h after inoculation, indicating that this PR is involved in the response of resistance to PepYMV in C. baccatum var. pendulum.

Snap bean recommendation based on different methods of phenotypic stability.

In order to recommend the best strains of snap beans from the Universidade Estadual do Norte Fluminense (UENF) breeding program, different methods of analysis of phenotypic stability were adopted to evaluate the performance of 14 lines (F(9) and F(10)) of indeterminate growth habit, which were compared with 3 controls, namely, 2 commercial varieties (Feltrin and Top Seed Blue Line) and 1 parent (UENF-1445). The experiments were conducted in Bom Jesus do Itabapoana in 2010 and 2011, and in Cambuci in 2011. The experiment was arranged in a randomized block design with 4 replications. To complement the information obtained by different methods, the UENF 7-5-1 strain was indicated for favorable environments (Bom Jesus do Itabapoana; 2010 and 2011), UENF 7-10-1, UENF 7-14-1, and UENF 7-20-1 strains were indicated for an unfavorable environment (Cambuci), and UENF 7-12-1 was indicated for both.

Genetic progress in the UNB-2U population of popcorn under recurrent selection in Rio de Janeiro, Brazil.

As part of the Universidade Estadual do Norte Fluminense recurrent selection program of popcorn, we evaluated full-sib families of the sixth cycle of recurrent selection and estimated genetic progress for grain yield and expansion capacity. We assessed 200 full-sib families for 10 agronomic traits, in a randomized block design, with two replications within sets in two environments: Campos dos Goytacazes and Itaocara, in the State of Rio de Janeiro, Brazil. There were significant differences for families/"sets" for all traits, indicating genetic variability that could be exploited in future cycles. In the selection of superior progenies, the Mulamba and Mock index gave the best gains for popping expansion (PE) and grain yield (GY), with values of 10.97 and 15.30%, respectively, using random economic weights. By comparing the evolution of the means obtained for PE and GY in the cycles C(0), C(1), C(2), C(3), C(4), C(5), and predicted for C(6), a steady increase was observed for both PE and GY, with the addition of 1.71 mL/g (R(2) = 0.93) and 192.87 kg/ha (R(2) = 0.88), respectively, in each cycle. Given the good performance of this popcorn population in successive cycles of intrapopulation recurrent selection, we expect that a productive variety with high expansion capacity will soon be available for producers in the north and northwest regions of Rio de Janeiro State, Brazil.

Use of transgenic seeds in Brazilian agriculture and concentration of agricultural production to large agribusinesses.

We identified the commercial releases of genetically modified organisms (GMOs) in Brazil, their characteristics, the types of genetic transformation used, and the companies responsible for the development of these GMOs, classifying them into two categories: private companies, subdivided into multinational and national, and public institutions. The data came from the data bank of the national registration of cultivars and the service of national protection of cultivars of the Ministry of Agriculture, Fishing and Supply (MAPA). This survey was carried out from 1998 to February 12, 2011. Until this date, 27 GMOs had been approved, including five for soybean, 15 for maize and seven for cotton cultivars. These GMOs have been used for the development of 766 cultivars, of which, 305 are soybean, 445 are maize, and 13 are cotton cultivars. The Monsato Company controls 73.2% of the transgenic cultivars certified by the MAPA; a partnership between Dow AgroSciences and DuPont accounts for 21.4%, and Syngenta controls 4.96%. Seed supply by these companies is almost a monopoly supported by law, giving no choice for producers and leading to the fast replacement of conventional cultivars by transgenic cultivars, which are expensive and exclude small producers from the market, since seeds cannot be kept for later use. This situation concentrates production in the hands of a few large national agribusiness entrepreneurs.

Full-sib reciprocal recurrent selection in the maize populations Cimmyt and Piranão.

We estimated the genetic gains of the 12th cycle of reciprocal recurrent selection for maize traits of agronomic interest. We used 23 ISSR molecular markers in an attempt to maximize genetic variability among and within populations based on selection of S(1) progenies. To this end, 138 full-sib families were evaluated in a randomized block design in two environments (the municipalities of Campos dos Goytacazes and Itaocara, in the State of Rio de Janeiro, Brazil), with replications within sets. Direct selection for grain yield was used for the selection of the families. To assess genetic diversity among and within populations, we examined plants produced from part of the S(1s) seeds from the parents that originated the 42 full-sib families that were selected from the agronomic traits. Direct selection for grain yield provided good gains for the traits evaluated, with estimated improvement of -0.87 days for days to flowering, 0.35 plants, 1.79 ears per plot, 0.58 g per 100-grain weight, 308.21 g ear weight per plot, and 261.83 kg/ha grain yield. Application of molecular markers at the stage of superior progeny selection led to increased genetic distance among populations, which is a very important factor for utilization of heterosis and providing greater longevity to the reciprocal recurrent selection program.

RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass.

Considering the expected genetic variability of elephant grass (Pennisetum purpureum), due to its cultivation in different continents, we characterized and estimated the genetic divergences between 46 accessions of elephant grass with different edaphoclimatic adaptations, using RAPD and ISSR markers. We evaluated, comparatively, the consistency of the information achieved with these markers. Twenty-six RAPD and 25 ISSR primers were employed. The RAPD markers produced 185 bands, 72% of which were polymorphic, with a mean of 5.11 polymorphic bands per primer. The 25 ISSR starters produced 216 bands; 76% were polymorphic, with a mean of 6.56 polymorphic bands per primer. The correlation between the genetic distances achieved by the RAPD and ISSR markers was 0.76, which is highly significant by the Mantel test. Based on UPGMA grouping, considering the point of sudden change, five and six groups were formed for the data from the RAPD and ISSR markers, respectively. These markers provided partially concordant groups, indicating that these techniques can provide consistent information and consequently could be used in studies of genetic diversity among accessions.

Revisiting the Brazilian scenario of registry and protection of cultivars: an analysis of the period from 1998 to 2010, its dynamics and legal observations.

During the last 20 years, the national production of grains has increased 156.1%; productivity increased 93.8% and there has been an increase of 29.1% in cultivated area. Currently, agribusiness is responsible for 40% of Brazilian exports. Nevertheless, there is little quantitative information on the main plant species of economic interest that have been registered and protected in the Agriculture, Fisheries and Food Supply Ministry (MAPA) by public and private companies, as well as by public-private partnerships. Consequently, we investigated the registry and protection of 27 species of economic interest, including the 15 that are the basis of the Brazilian diet, based on the information available on the site CultivarWeb, of MAPA, for the period from 1998 to August 30, 2010. We also examined the legislation that regulates registration and protection procedures and its implications for plant breeding and plant product development. It was found that the private sector controls 73.1% of the registrations and 53.56% of the protections, while 10.73% of the protections were of material developed overseas. Public-private partnerships contributed little to the development of new cultivars, with 0.5% of the registries and 3.61% of the protections. We conclude that plant protection directed private investment to development of wheat and rice varieties, with the greatest public investments directed to corn and sorghum. After the Cultivar Protection Law was implemented, there was restriction of access to germplasm banks, which could inhibit advances in Brazilian plant breeding programs, indicating a need for revision of this legal barrier.

Combining ability of tropical maize lines for seed quality and agronomic traits.

Studies of genetic effects of early selection of maize based on seed quality traits are rare, especially those that use materials from different heterotic groups. These studies are also useful in tropical environments and for the advancement of sustainable agriculture with cropping during seasons not commonly used for cultivation. We estimated, through diallel crosses, the predominant genetic effects on the expression of agronomic traits and seed quality and on the general combining ability of nine maize lines from commercial hybrids and the specific combining ability of hybrid combinations among them. In the evaluation of seed quality, seven tests were used: first count and final count of seed germination, seedling vigor classification, cold tolerance, seedling emergence rate in a sand seedbed, speed of emergence in a sand seedbed, and speed of emergence index. Plant height, first ear height and grain yield were the estimated agronomic traits. In the diallel analysis, method 3 (model I) proposed by Griffing was used. There was a greater significance of non-additive genetic effects in the genetic control of seed quality of the various lines. The Flash, Dekalb 350 and P 30F80 lines combined high seed quality and high grain yield. For growth during the normal planting season, the combinations CD 3121-1 x P 30F80, Speed x CD 3121-2, Dow 8330 x AG 8080 and Dekalb 350 x CD 3121-2 were the most promising for both seed quality and agronomic traits.