Antibiotics and microbial community-induced antibiotic-resistant genes distribution in soil and sediment in the eastern coastline of China.

The health risk of antibiotic-resistant genes (ARGs) has been a global concern, while the report on occurrence and prevalence of ARGs in coastal zone is relatively scarce. This study investigated typical ARGs in soil and sediment in coastal line of eastern China and assessed its relationship with antibiotics and heavy metals as well as microbial community. Results showed that eight ARGs were all detected in the samples, and ß-lactam resistance gene blaTEM reached the highest absolute abundance (6.28 × 107 ~ 6.48 × 108 copies/g) and relative abundance (2.3 × 10-2 copies/16S rRNA) among samples. Amoxicillin and tetracycline were most frequently detected with the average concentration of 2.28 µg/kg and 3.48 µg/kg, respectively. Cr and Zn were found to be most abundant heavy metals with average value of 82.1 and 59.1 mg/kg, respectively. Proteobacteria, Campilobacterota, Bacteroidota, and Firmicutes were dominant phyla in most samples, while bacterial community varied significantly among samples. Redundancy analyses (RDA) showed that microbial community and antibiotics (amoxicillin and tetracycline) were driving factors of ARGs distribution, while heavy metals were not significantly correlated with ARGs. This study is helpful to understand the fate of ARGs in coastal zone.

An electrochemical aptasensor for the milk allergen ß-lactoglobulin detection based on a target-induced nicking site reconstruction strategy.

Food allergy is an immune system reaction to a particular food, milk being the most common one. ß-Lactoglobulin (ß-Lg) is the main ingredient of milk protein and the main cause of infant milk allergy. On such an occasion, the determination of ß-Lg is very important and the electrochemical sensors are a good alternative for this purpose since they are sensitive, selective and inexpensive. In this work, an electrochemical aptasensor was fabricated for the quantitative detection of ß-Lg in hypoallergenic formula (HF) milk. A tri-functional hairpin (HP) was designed, which was composed of an aptamer sequence, a nicking site and a DNA sequence (T1). In the absence of ß-Lg, the aptamer part hybridized with T1 to form a stable stem-loop structure. However, in the presence of ß-Lg, the capture of the aptamer sequence towards ß-Lg caused the reconstruction of HP and thus the nicking sites were exposed. Then, the nicking enzyme was activated and T1 could be released, which bound with the end of the hairpin 1-methylene blue (HP1-MB)/HP2-MB conjugation on the Au nanoparticle (AuNP) modified electrode surface. Thus, the insulating property of the electrode was enhanced and the current response of MB decreased, which built the quantitative basis for ß-Lg detection. In this way, the proposed aptasensor exhibited a wide linear range of 0.01-100 ng mL-1 and a low detection limit of 5.7 pg mL-1. This aptasensor also displayed high selectivity, reproducibility and stability, and became a promising platform for ß-Lg detection in real food samples.

Controlled Synthesis of Ag2 Te@Ag2 S Core-Shell Quantum Dots with Enhanced and Tunable Fluorescence in the Second Near-Infrared Window.

Fluorescence in the second near-infrared window (NIR-II, 900-1700 nm) has drawn great interest for bioimaging, owing to its high tissue penetration depth and high spatiotemporal resolution. NIR-II fluorophores with high photoluminescence quantum yield (PLQY) and stability along with high biocompatibility are urgently pursued. In this work, a Ag-rich Ag2 Te quantum dots (QDs) surface with sulfur source is successfully engineered to prepare a larger bandgap of Ag2 S shell to passivate the Ag2 Te core via a facile colloidal route, which greatly enhances the PLQY of Ag2 Te QDs and significantly improves the stability of Ag2 Te QDs. This strategy works well with different sized core Ag2 Te QDs so that the NIR-II PL can be tuned in a wide range. In vivo imaging using the as-prepared Ag2 Te@Ag2 S QDs presents much higher spatial resolution images of organs and vascular structures as compared with the same dose of Ag2 Te nanoprobes administrated, suggesting the success of the core-shell synthetic strategy and the potential biomedical applications of core-shell NIR-II nanoprobes.

Emerging and legacy perfluoroalkyl and polyfluoroalkyl substances (PFAS) in surface water around three international airports in China.

Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are a large category of crucial environmental contaminants of global concerns. There are limited data on PFAS in surface water around international airports in China. The present study investigated the concentrations, distributions, and sources of emerging and legacy PFAS in surface waters around Beijing Capital International Airport (BC), Shanghai Pudong International Airport (SP), and Guangzhou Baiyun International Airport (GB) in China. Twenty-seven target compounds were quantified. The Σ27PFAS concentrations ranged from 19.0 to 62.8 ng/L (mean 36.1 ng/L) in BC, 25.6-342 ng/L (mean 76.0 ng/L) in SP, 7.35-72.7 ng/L (mean 21.6 ng/L) in GB. The dominant compound was perfluorooctanoic acid (PFOA), which accounted for an average of 27% (5%-65%) of the Σ27PFAS concentrations. The alternatives with -C6F12- group had detection frequencies ranging from 72% to 100%. The partition coefficient results indicate that the longer chain PFAS (C > 8) tend to be more distributed in the particle phase. Fifty suspect and nontarget PFAS were identified. In GB, 44 PFAS were identified, more than SP of 39 and BC of 38. An ultra short-chain (C = 2) precursor, N-methylperfluoroethanesulfonamido acetic acid (MeFEtSAA), was identified and semi-quantified. Domestic wastewater discharges might be the main sources around BC, while industrial and aviation activities might be the main sources around SP and GB.

Mechanical influence of tissue scaffolding design with different geometries using finite element study.

The mechanical properties of tissue scaffolds are essential in providing stability for tissue repair and growth. Thus, the ability of scaffolds to withstand specific loads is crucial for scaffold design. Most research on scaffold pores focuses on grids with pore size and gradient structure, and many research models are based on scaffolding with vertically arranged holes. However, little attention is paid to the influence of the distribution of holes on the mechanical properties of the scaffold. To address this gap, this research investigates the effect of pore distribution on the mechanical properties of tissue scaffolds. The study involves four types of scaffold designs with regular and staggered pore arrangements and porosity ranging from 30% to 80%. Finite element analysis (FEA) was used to compare the mechanical properties of different scaffold designs, with von-Mises stress distribution maps generated for each scaffold. The results show that scaffolds with regular vertical holes exhibit a more uniform stress distribution and better mechanical performance than those with irregular holes. In contrast, the scaffold with a staggered arrangement of holes had a higher probability of stress concentration. The study emphasized the importance of balancing porosity and strength in scaffold design.

Facile synthesis of noncytotoxic PEGylated dendrimer encapsulated silver sulfide quantum dots for NIR-II biological imaging.

Near-infrared-II (NIR-II, 1000-1700 nm) bioimaging features high penetration depth and high spatio-temporal resolution compared to traditional fluorescence imaging, but the key is to develop stable and biocompatible NIR-II fluorophores suitable for in vivo applications. Silver sulfide quantum dots (Ag2S QDs) have been demonstrated to be excellent for in vivo NIR-II imaging with unique optical properties and decent biocompatibility, but they often require complex post modifications for in vivo applications. Herein we demonstrate a facile one-pot strategy to synthesize PEGylated dendrimer-encapsulated Ag2S QDs useful for in vivo NIR-II imaging. Silver ions were first loaded into the core of an acylthiourea-functionalized dendrimer (PEG-PATU) through coordination between silver ions and acylthiourea groups, followed by the addition of sodium sulfide to form Ag2S QDs in situ. The resulting PEG-PATU Ag2S QDs exhibit excellent NIR-II fluorescence signals, and thus could be used for high efficiency labelling and tracking of A549 cancer cell mobility in vivo and real time visualization of the vast circulatory network of a mouse.