Browning-induced changes in trophic functioning of planktonic food webs in temperate and boreal lakes: insights from fatty acids.

The effects of lake browning on trophic functioning of planktonic food webs are not fully understood. We studied the effects of browning on the response patterns of polyunsaturated fatty acids and n-3/n-6 ratio in seston and compared them between boreal and temperate lakes. We also compared the regional differences and the effects of lake browning on the reliance of zooplankton on heterotrophic microbial pathways and the mass fractions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in zooplankton. Lake browning was associated with increasing phytoplankton biomass and concentrations of EPA and DHA in both temperate and boreal lakes, but the seston n-3/n-6 ratio was lower in temperate than boreal lakes, most likely due the differences in phytoplankton community composition. The browning-induced increase in phytoplankton biomass was associated with increased reliance of zooplankton on a heterotrophic microbial pathway for both cladocerans and copepods in boreal and temperate lakes. This increased reliance on the heterotrophic microbial diet was correlated with a decrease in the EPA and DHA mass fractions in temperate copepods and a decrease in the n-3/n-6 ratio in boreal cladocerans and copepods. Our results indicate that although phytoplankton responses to lake browning were similar across regions, this did not directly cascade to the next trophic level, where zooplankton responses were highly taxa- and region-specific. These results indicate that lake browning should be considered as an overarching moderator that is linked to, e.g., nutrient increases, which have more immediate consequences on trophic interactions at the phytoplankton-zooplankton interface.

Parasites and their freshwater snail hosts maintain their nutritional value for essential fatty acids despite altered algal diets.

Despite their ubiquity and considerable biomass, the roles played by parasites in aquatic food webs are still not well understood, especially those of their free-living infectious stages. For instance, cercariae, the motile larvae of parasitic flukes (trematodes) may be a key source of nutrients and energy for consumers. As cercariae clonally reproduce within the digestive-gonadal gland complex of gastropod intermediate hosts that acquire nutritionally important polyunsaturated fatty acids (PUFA) mainly from their diets (e.g., by grazing on primary producers), cercariae could transfer snail-derived PUFA if consumed. Through fatty acid (FA) analysis, we explored whether a change in the diet of parasitized hosts altered the FA profiles of both snail-only and trematode-containing snail tissue, thereby affecting their nutritional values. Freshwater snails (Stagnicola elodes) infected with Plagiorchis sp. were fed three different diets (cyanobacteria, green algae, and diatoms) that differed in nutritional quality with respect to FA profiles. While diet influenced the overall FA composition of both snail-only tissue and snail tissue containing trematodes, levels of certain PUFA (mainly omega-3) were largely unaffected. Trematode-containing snail tissue also generally contained more PUFA relative to snail-only tissue. Notably, both tissue types had far higher levels of PUFA than found in their diets. Our results suggest that freshwater snail hosts, and possibly their associated trematode parasites, could be trophic upgraders of key PUFA despite anthropogenically induced changes in algal communities that may lead to overall diminished PUFA contents. As such, cercariae-mediated trophic transfers of PUFA may play important roles in aquatic food webs.

Impacts of water level fluctuations on mercury concentrations in hydropower reservoirs: A microcosm experiment.

Hydropower generation, a renewable source of electricity, has been linked to elevated methylmercury (MeHg) concentrations in impoundments and aquatic biota. This study investigates the impact of water level fluctuations (WLF) on MeHg concentrations in water, sediment, and fish. Using a set of controlled microcosm experiments emulating the drawdown/refill dynamics and subsequent sediment exposure to air experienced in reservoirs, we demonstrate that less frequent WLFs, and/or increased exposure of sediment to air, can lead to elevated MeHg concentrations in sediment, and total mercury (THg) and MeHg concentrations in water. In examining the effects of WLF frequency (two-day, weekly, and monthly), the monthly treatment displayed the highest THg and MeHg water levels, while the weekly treatment was characterized by the highest MeHg levels in the sediment. Our work supports emerging evidence that longer duration between WLF creates a larger surface area of sediment exposed to air leading to conditions conducive to higher MeHg concentrations in sediments and water. In contrast, THg, MeHg, and fatty acid trends in fish were largely inconclusive characterized by similar among-treatment effects and minimal temporal variability over the course of our experiment. This result could partly be attributed to overall low mercury levels and simple "worm-forage fish" food web in our experiment. To elucidate the broader impacts of water fluctuations on aquatic chemistry and biota, other factors (e.g., longer WLF cycles, dissolved organic matter, temperature, more complex food webs) which modulate both methylation rates and food web dynamics must be considered.

Feeding habitat and silvering stage affect lipid content and fatty acid composition of European eel Anguilla anguilla tissues.

Lipids, particularly fatty acids (FAs), are major sources of energy and nutrients in aquatic ecosystems and play key roles during vertebrate development. The European eel Anguilla anguilla goes through major biochemical and physiological changes throughout its lifecycle as it inhabits sea- (SW), and/or brackish- (BW) and/or freshwater (FW) habitats. With the ultimate goal being to understand the reasons for eels adopting a certain life history strategy (FW or SW residency vs. 'habitat shifting'), we explored differences in lipid content and FA composition of muscle, liver and eyes from eels collected across Norwegian SW, BW and FW habitats, and at different lifecycle stages (yellow to silver). FW and SW eels had a higher lipid content overall compared to BW eels, reflecting differences in food availability and life history strategies. SW eels had higher proportions of certain monounsaturated FAs (MUFAs; 18:1n-9, 20:1n-9), and of the essential polyunsaturated FAs 20:5n-3 (eicosapentaenoic acid, EPA) and 22:6n-3 (docosahexaenoic acid) than FW eels, reflecting a marine-based diet. In contrast, the muscle of FW eels had higher proportions of 18:3n-3, 18:2n-6 and 20:4n-6 (arachidonic acid), as is typical of FW organisms. MUFA proportions increased in later stage eels, consistent with the hypothesis that the eels accumulate energy stores prior to migration. In addition, the decrease of EPA with advancing stage may be associated with the critical role that this FA plays in eel sexual development. Lipid and FA information provided further understanding of the habitat use and overall ecology of this critically endangered species.

Parasite infectious stages provide essential fatty acids and lipid-rich resources to freshwater consumers.

Free-living parasite infectious stages, such as motile cercariae of trematodes (flatworms), can constitute substantial biomass within aquatic ecosystems and are frequently eaten by various consumers, potentially serving as an important source of nutrients and energy. However, quantitative data on their nutritional value (e.g., essential fatty acids [EFA]) are largely lacking. As EFA are leading indicators of nutritional quality and underpin aquatic ecosystem productivity, we performed fatty acid (FA) analysis on an aggregate of ~ 30,000 cercariae of the freshwater trematode, Ribeiroia ondatrae. Individual cercariae contained 15 ng of total FA, and considerable quantities of EFA, including eicosapentaenoic (EPA, at 0.79 ng cercaria-1) and docosahexaenoic (DHA, at 0.01 ng cercaria-1) acids. We estimated annual EFA production by R. ondatrae cercariae for a series of ponds in California to be 40.4-337.0 µg m-2 yr-1 for EPA and 0.7-6.2 µg m-2 yr-1 for DHA. To investigate viability of cercariae as prey, we also compared growth and FA profiles of dragonfly larvae (naiads of Leucorrhinia intacta) fed equivalent masses of either R. ondatrae or zooplankton (Daphnia spp.) for 5 weeks. Naiads raised on the two diets grew equally well, with no significant differences found in their EFA profiles. While zooplankton are widely recognized as a vital source of energy, and an important conduit for the movement of EFA between algae and higher trophic levels, we suggest a similar role for trematode cercariae by 'unlocking' EFA from the benthic environment, highlighting their potential importance as a nutrient source that supports animal health.

Genetically Engineered Oil Seed Crops and Novel Terrestrial Nutrients: Ethical Considerations.

Genetically engineered (GE) organisms have been at the center of ethical debates among the public and regulators over their potential risks and benefits to the environment and society. Unlike the currently commercial GE crops that express resistance or tolerance to pesticides or herbicides, a new GE crop produces two bioactive nutrients (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) that heretofore have largely been produced only in aquatic environments. This represents a novel category of risk to ecosystem functioning. The present paper describes why growing oilseed crops engineered to produce EPA and DHA means introducing into a terrestrial ecosystem a pair of highly bioactive nutrients that are novel to terrestrial ecosystems and why that may have ecological and physiological consequences. More importantly perhaps, this paper argues that discussion of this novel risk represents an opportunity to examine the way the debate over genetically modified crops is being conducted.

Compound-specific isotope analysis resolves the dietary origin of docosahexaenoic acid in the mouse brain.

DHA (22:6n-3) may be derived from two dietary sources, preformed dietary DHA or through synthesis from α-linolenic acid (ALA; 18:3n-3). However, conventional methods cannot distinguish between DHA derived from either source without the use of costly labeled tracers. In the present study, we demonstrate the proof-of-concept that compound-specific isotope analysis (CSIA) by GC-isotope ratio mass spectrometry (IRMS) can differentiate between sources of brain DHA based on differences in natural 13C enrichment. Mice were fed diets containing either purified ALA or DHA as the sole n-3 PUFA. Extracted lipids were analyzed by CSIA for natural abundance 13C enrichment. Brain DHA from DHA-fed mice was significantly more enriched (-23.32‰ to -21.92‰) compared with mice on the ALA diet (-28.25‰ to -27.49‰). The measured 13C enrichment of brain DHA closely resembled the dietary n-3 PUFA source, -21.86‰ and -28.22‰ for DHA and ALA, respectively. The dietary effect on DHA 13C enrichment was similar in liver and blood fractions. Our results demonstrate the effectiveness of CSIA, at natural 13C enrichment, to differentiate between the incorporation of preformed or synthesized DHA into the brain and other tissues without the need for tracers.

Climate warming is predicted to reduce omega-3, long-chain, polyunsaturated fatty acid production in phytoplankton.

Phytoplankton are the main source of energy and omega-3 (n-3) long-chain essential fatty acids (EFA) in aquatic ecosystems. Their growth and biochemical composition are affected by surrounding environmental conditions, including temperature, which continues to increase as a result of climate warming. Increasing water temperatures may negatively impact the production of EFA by phytoplankton through the process of homeoviscous adaptation. To investigate this, we conducted an exploratory data synthesis with 952 fatty acid (FA) profiles from six major groups of marine and freshwater phytoplankton. Temperature was strongly correlated with a decrease in the proportion of n-3 long-chain polyunsaturated FA (LC-PUFA) and an increase in omega-6 FA and saturated FA. Based on linear regression models, we predict that global n-3 LC-PUFA production will be reduced by 8.2% for eicosapentaenoic acid (EPA) and 27.8% for docosahexaenoic acid (DHA) with an increase in water temperature of 2.5 °C. Using a previously published estimate of the global production of EPA by diatoms, which contribute to most of the world's supply of EPA, we predict a loss of 14.2 Mt of EPA annually as a result of ocean warming. The n-3 LC-PUFA are vitally important for an array of key physiological functions in aquatic and terrestrial organisms, and these FA are mainly produced by phytoplankton. Therefore, reduced production of these EFA, as a consequence of climate warming, is predicted to negatively affect species that depend on these compounds for optimum physiological function. Such profound changes in the biochemical composition of phytoplankton cell membranes can lead to cascading effects throughout the world's ecosystems.

The jellification of north temperate lakes.

Calcium (Ca) concentrations are decreasing in softwater lakes across eastern North America and western Europe. Using long-term contemporary and palaeo-environmental field data, we show that this is precipitating a dramatic change in Canadian lakes: the replacement of previously dominant pelagic herbivores (Ca-rich Daphnia species) by Holopedium glacialis, a jelly-clad, Ca-poor competitor. In some lakes, this transformation is being facilitated by increases in macro-invertebrate predation, both from native (Chaoborus spp.) and introduced (Bythotrephes longimanus) zooplanktivores, to which Holopedium, with its jelly coat, is relatively invulnerable. Greater representation by Holopedium within cladoceran zooplankton communities will reduce nutrient transfer through food webs, given their lower phosphorus content relative to daphniids, and greater absolute abundances may pose long-term problems to water users. The dominance of jelly-clad zooplankton will likely persist while lakewater Ca levels remain low.

Arcobacter lanthieri sp. nov., isolated from pig and dairy cattle manure.

A study was undertaken to determine the prevalence and diversity of species of the genus Arcobacter in pig and dairy cattle manure, which led to the identification of strains AF1440T, AF1430 and AF1581. Initially identified as Arcobacter butzleri based on colony morphology and initial PCR-confirmation tests, analyses of 16S rRNA gene sequences of these strains confirmed that they belonged to the genus Arcobacter and were different from all known species of the genus. The isolates formed a distinct group within the genus Arcobacter based on their 16S rRNA, gyrB, rpoB, cpn60, gyrA and atpA gene sequences and fatty acid profiles. Their unique species status was further supported by physiological properties and DNA-DNA hybridization that allowed phenotypic and genotypic differentiation of the strains from other species of the genus Arcobacter. The isolates were found to be oxidase, catalase and esterase positive and urease negative; they grew well at 30 °C under microaerophilic conditions and produced nitrite and acetoin. Based on their common origin and various physiological properties, it is proposed that the isolates are classified as members of a novel species with the name Arcobacter lanthieri sp. nov. The type strain is AF1440T ( = LMG 28516T = CCUG 66485T); strains AF1430 ( = LMG 28515 = CCUG 66486) and AF1581 ( = LMG 28517 = CCUG 66487) are reference strains.

Risk-benefit of consuming Lake Erie fish.

BACKGROUND: Consumption of fish is promoted as a healthy way to obtain essential fatty acids (EFA) in the diet, yet the risk of ingesting harmful contaminants remains a concern. A recent study concluded that the risk-benefit of consuming fish from the North American Laurentian Great Lakes, which sustain important commercial and recreational fisheries, is currently unclear. We report the fatty acid (FA) content in skin-off fillets of fifteen fish species from Lake Erie and assess whether recommended dietary requirements for two EFA (EPA and DHA) can be met by safely consuming Lake Erie fishes, as an example of a risk-benefit analysis. METHODS: A total of 146 samples were analyzed for FA and contaminant content. A simulated fish consumption advisory (maximum recommended number of meals per month, up to 32) was calculated for each sample, and used to calculate the maximum amount of EPA+DHA that would be consumed if the consumption advisory was followed. RESULTS: All fifteen species had nutritionally desirable PUFA:SAFA (>0.4) and n-3:n-6 (>1). Large, fatty species had the highest EPA+DHA content, but had the most restrictive consumption advisories due to high PCB concentrations. To minimize contaminant exposure while maximizing EPA+DHA intake, consumers should consider small lake whitefish and lake trout, small panfish species, and/or walleye. However, very few species had an EPA+DHA content sufficient to safely meet the highest dietary guidelines while following advisories. CONCLUSIONS: Consumption of certain Lake Erie fish, an important recreational and commercial fishery, within the limits of our simulated fish consumption advisories, can be a good supplemental source of beneficial n-3 long chain PUFA.

Nutrient loading associated with agriculture land use dampens the importance of consumer-mediated niche construction.

The linkages between biological communities and ecosystem function remain poorly understood along gradients of human-induced stressors. We examined how resource provisioning (nutrient recycling), mediated by native freshwater mussels, influences the structure and function of benthic communities by combining observational data and a field experiment. We compared the following: (1) elemental and community composition (algal pigments and macroinvertebates) on live mussel shells and on nearby rocks across a gradient of catchment agriculture and (2) experimental colonisation of benthic communities on live vs. sham shells controlling for initial community composition and colonisation duration. We show that in near pristine systems, nutrient heterogeneity mediated by mussels relates to greater biodiversity of communities, which supports the notion that resource heterogeneity can foster biological diversity. However, with increased nutrients from the catchment, the relevance of mussel-provisioned nutrients was nearly eliminated. While species can persist in disturbed systems, their functional relevance may be diminished or lost.

Seasonal pollutant levels in littoral high-Arctic amphipods in relation to food sources and terrestrial run-off.

Increasing terrestrial run-off from melting glaciers and thawing permafrost to Arctic coastal areas is expected to facilitate re-mobilization of stored legacy persistent organic pollutants (POPs) and mercury (Hg), potentially increasing exposure to these contaminants for coastal benthic organisms. We quantified chlorinated POPs and Hg concentrations, lipid content and multiple dietary markers, in a littoral deposit-feeding amphipod Gammarus setosus and sediments during the melting period from April to August in Adventelva river estuary in Svalbard, a Norwegian Arctic Aarchipelago. There was an overall decrease in concentrations of ∑POPs from April to August (from 58 ± 23 to 13 ± 4 ng/g lipid weight; lw), Hg (from 5.6 ± 0.7 to 4.1 ± 0.5 ng/g dry weight; dw) and Methyl Hg (MeHg) (from 5 ± 1 to 0.8 ± 0.7 ng/g dw) in G. setosus. However, we observed a seasonal peak in penta- and hexachlorobenzene (PeCB and HCB) in May (2.44 ± 0.3 and 23.6 ± 1.7 ng/g lw). Sediment concentrations of POPs and Hg (dw) only partly correlated with the contaminant concentrations in G. setosus. Dietary markers, including fatty acids and carbon and nitrogen stable isotopes, indicated a diet of settled phytoplankton in May-July and a broader range of carbon sources after the spring bloom. Phytoplankton utilization and chlorobenzene concentrations in G. setosus exhibited similar seasonal patterns, suggesting a dietary uptake of chlorobenzenes that is delivered to the aquatic environment during spring snowmelt. The seasonal decrease in contaminant concentrations in G. setosus could be related to seasonal changes in dietary contaminant exposure and amphipod ecology. Furthermore, this decrease implies that terrestrial run-off is not a significant source of re-mobilized Hg and legacy POPs to littoral amphipods in the Adventelva river estuary during the melt season.

Additive effects of enhanced ambient ultraviolet B radiation and increased temperature on immune function, growth and physiological condition of juvenile (parr) Atlantic Salmon, Salmo salar.

Climate change models predict increased ultraviolet B (UVB) radiation levels due to stratospheric ozone depletion and global warming. In order to study the impact of these two environmental stressors acting simultaneously on the physiology of fish, Atlantic salmon parr were exposed, for 8 weeks in outdoor tanks, to different combinations of UVB radiation (depleted and enhanced) and temperature (standard rearing temperature of 14 °C or 19 °C). The immune function (plasma IgM, lysozyme activity and complement bacteriolytic activity), growth (body weight) and physiological condition (haematocrit and plasma protein concentration) of the fish were determined. Increased UVB level, regardless of water temperature, had a negative effect on immune function parameters, growth and physiological condition. Higher temperature increased plasma IgM concentration but had a negative effect on complement bacteriolytic activity under both spectral treatments. Increased temperature, irrespective of UVB level, increased fish growth but negatively affected haematocrit and plasma protein. Exposing the fish to enhanced UVB at elevated temperature increased plasma IgM concentration and slightly improved growth. However, complement activity and physiological condition parameters decreased more than when the fish were exposed to each stressor separately. The changes were mainly additive; no interactive or synergistic effects were observed. The negative impact of multiple stressors on immune function, together with predicted increases in pathogen load in warmer waters resulting from global climate change, suggest an increased risk to diseases in fishes.

Omega-3 PUFA profoundly affect neural, physiological, and behavioural competences - implications for systemic changes in trophic interactions.

In recent decades, much conceptual thinking in trophic ecology has been guided by theories of nutrient limitation and the flow of elements, such as carbon and nitrogen, within and among ecosystems. More recently, ecologists have also turned their attention to examining the value of specific dietary nutrients, in particular polyunsaturated fatty acids (PUFA), among which the omega-3 PUFA, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) play a central role as essential components of neuronal cell membranes in many organisms. This review focuses on a new neuro-ecological approach stemming from the biochemical (mechanistic) and physiological (functional) role of DHA in neuronal cell membranes, in particular in conjunction with G-protein coupled receptors (GPCRs). We link the co-evolution of these neurological functions to metabolic dependency on dietary omega-3 PUFA. We outline ways in which deficiencies in dietary DHA supply may affect, cognition, vision, and behaviour, and ultimately, the biological fitness of consumers. We then review emerging evidence that changes in access to dietary omega-3 PUFA may ultimately have profound impacts on trophic interactions leading to potential changes in community structure and ecosystem functioning that, in turn, may affect the supply of DHA within and across ecosystems, including the supply for human consumption.

Schizochytrium sp. (T18) Oil as a Fish Oil Replacement in Diets for Juvenile Rainbow Trout (Oncorhynchus mykiss): Effects on Growth Performance, Tissue Fatty Acid Content, and Lipid-Related Transcript Expression.

In this study, we evaluated whether oil extracted from the marine microbe, Schizochytrium sp. (strain T18), with high levels of docosahexaenoic acid (DHA), could replace fish oil (FO) in diets for rainbow trout (Oncorhynchus mykiss). Three experimental diets were tested: (1) a control diet with fish oil (FO diet), (2) a microbial oil (MO) diet with a blend of camelina oil (CO) referred to as MO/CO diet, and (3) a MO diet (at a higher inclusion level). Rainbow trout (18.8 ± 2.9 g fish-1 initial weight ± SD) were fed for 8 weeks and evaluated for growth performance, fatty acid content and transcript expression of lipid-related genes in liver and muscle. There were no differences in growth performance measurements among treatments. In liver and muscle, eicosapentaenoic acid (EPA) was highest in trout fed the FO diet compared to the MO/CO and MO diets. Liver DHA was highest in trout fed the MO/CO diet compared to the FO and MO diets. Muscle DHA was highest in trout fed the MO and MO/CO diets compared to the FO diet. In trout fed the MO/CO diet, compared to the MO diet, fadsd6b was higher in both liver and muscle. In trout fed the FO or MO/CO diets, compared to the MO diet, cox1a was higher in both liver and muscle, cpt1b1a was higher in liver and cpt1a1a, cpt1a1b and cpt1a2a were higher in muscle. Schizochytrium sp. (T18) oil was an effective source of DHA for rainbow trout.

Characterization of Pseudomonas aeruginosa fatty acid profiles in biofilms and batch planktonic cultures.

The fatty acid composition of Pseudomonas aeruginosa PAO1 was compared between biofilm and batch planktonic cultures. Strain PAO1 biofilms were able to maintain a consistent fatty acid profile for up to 6 days, whereas strain PAO1 batch planktonic cultures showed a gradual loss of cis-monounsaturated fatty acids over 4 days. Biofilms exhibited a greater proportion of hydroxy fatty acids but a lower proportion of both cyclopropane fatty acids and saturated fatty acids (SAFAs). SAFAs with >=16 carbons, in particular, decreased in biofilms when compared with that in batch planktonic cultures. A reduced proportion of SAFAs and a decline in overall fatty acid chain length indicate more fluidic biophysical properties for cell membranes of P. aeruginosa in biofilms. Separating the biofilms into 2 partitions and comparing their fatty acid compositions revealed additional trends that were not observed in the whole biofilm: the shear-nonremovable layer consistently showed greater proportions of hydroxy fatty acid than the bulk liquid + shear-removable portion of the biofilm. The shear-nonremovable portion demonstrated a relatively immediate decline in the proportion of monounsaturated fatty acids between days 2 and 4; which was offset by an increase in the proportion of cyclopropane fatty acids, specifically 19:0cyc(11,12). Simultaneously, the shear-removable portion of the biofilm showed an increase in the proportion of trans-monounsaturated fatty acids and cyclopropane fatty acids.

Projected declines in global DHA availability for human consumption as a result of global warming.

Docosahexaenoic acid (DHA) is an essential, omega-3, long-chain polyunsaturated fatty acid that is a key component of cell membranes and plays a vital role in vertebrate brain function. The capacity to synthesize DHA is limited in mammals, despite its critical role in neurological development and health. For humans, DHA is most commonly obtained by eating fish. Global warming is predicted to reduce the de novo synthesis of DHA by algae, at the base of aquatic food chains, and which is expected to reduce DHA transferred to fish. We estimated the global quantity of DHA (total and per capita) currently available from commercial (wild caught and aquaculture) and recreational fisheries. The potential decrease in the amount of DHA available from fish for human consumption was modeled using the predicted effect of established global warming scenarios on algal DHA production and ensuing transfer to fish. We conclude that an increase in water temperature could result, depending on the climate scenario and location, in a ~ 10 to 58% loss of globally available DHA by 2100, potentially limiting the availability of this critical nutrient to humans. Inland waters show the greatest potential for climate-warming-induced decreases in DHA available for human consumption. The projected decrease in DHA availability as a result of global warming would disproportionately affect vulnerable populations (e.g., fetuses, infants), especially in inland Africa (due to low reported per capita DHA availability). We estimated, in the worst-case scenario, that DHA availability could decline to levels where 96% of the global population may not have access to sufficient DHA.

Biochemical tracers reveal intra-specific differences in the food webs utilized by individual seabirds.

Food web structure regulates the pathways and flow rates of energy, nutrients, and contaminants to top predators. Ecologically and physiologically meaningful biochemical tracers provide a means to characterize and quantify these transfers within food webs. In this study, changes in the ratios of stable N isotopes (e.g., delta(15)N), fatty acids (FA), and persistent contaminants were used to trace food web pathways utilized by herring gulls (Larus argentatus) breeding along the shores of the St Lawrence River, Canada. Egg delta(15)N values varied significantly among years and were used as an indicator of gull trophic position. Temporal trends in egg delta(15)N values were related to egg FA profiles. In years when egg delta(15)N values were greater, egg FA patterns reflected the consumption of more aquatic prey. Egg delta(15)N values were also correlated with annual estimates of prey fish abundance. These results indicated that temporal changes in aquatic prey availability were reflected in the gull diet (as inferred from ecological tracer profiles in gull eggs). Analysis of individual eggs within years confirmed that birds consuming more aquatic prey occupied higher trophic positions. Furthermore, increases in trophic position were associated with increased concentrations of most persistent organic contaminants in eggs. However, levels of highly brominated polybrominated diphenyl ether congeners, e.g, 2,2',3,3',4,4',5,5',6,6'-decabromoDE (BDE-209), showed a negative relationship with trophic position. These contrasting findings reflected differences among contaminant groups/homologs in terms of their predominant routes of transfer, i.e., aquatic versus terrestrial food webs. High trophic level omnivores, e.g., herring gulls, are common in food webs. By characterizing ecological tracer profiles in such species we can better understand spatial, temporal, and individual differences in pathways of contaminant, energy, and nutrient flow.

Terrestrial organic matter increases zooplankton methylmercury accumulation in a brown-water boreal lake.

Increases in terrestrial organic matter (tOM) transport from catchments to boreal lakes can affect methylmercury (MeHg) accumulation in aquatic biota both directly by increasing concentrations of aqueous MeHg, and indirectly through effects on MeHg bioavailability and on energy pathways in the lower food web. We carried out a detailed seasonal study of water chemistry, zooplankton diet, and MeHg accumulation in zooplankton in two lakes with contrasting tOM concentrations. Between-lake differences explained 51% of the variability in our water chemistry data, with no observed effect of season or sampling depth, contrary to our expectations. Higher tOM was correlated with higher aqueous Hg concentrations, lower areal pelagic primary productivity, and an increased contribution of terrestrial particles to pelagic particulate organic matter. Based on dietary marker analysis (δ13C, δ15N, and fatty acid [FA] composition), zooplankton diet was strongly linked to feeding mechanism, with dietary reliance on phytoplankton highest in the selective-feeding calanoid copepods, and lowest in filter feeding cladocerans. Zooplankton dietary reliance on phytoplankton and their concentrations of high-quality lipids, including polyunsaturated fatty acids, were higher in the clear-water lake than in the brown-water lake, where bacterial and terrestrial food sources were more prevalent. MeHg was highest in zooplankton from the brown-water lake, with highest concentrations in the 200-500 µm zooplankton size fraction for both lakes. Contrary to our expectations, there was no effect of season on zooplankton dietary markers or MeHg. Our results suggest that, overall, higher tOM results in higher MeHg concentrations in water and zooplankton, and reduces zooplankton dietary reliance on phytoplankton. Increased tOM thus leads to a decrease in the nutritional quality of zooplankton (i.e. higher MeHg concentrations, and lower concentrations of essential fatty acids), which may cascade up the food web with negative implications for higher trophic levels.