The emergence and diversification of a zoonotic pathogen from within the microbiota of intensively farmed pigs.

The expansion and intensification of livestock production is predicted to promote the emergence of pathogens. As pathogens sometimes jump between species, this can affect the health of humans as well as livestock. Here, we investigate how livestock microbiota can act as a source of these emerging pathogens through analysis of Streptococcus suis, a ubiquitous component of the respiratory microbiota of pigs that is also a major cause of disease on pig farms and an important zoonotic pathogen. Combining molecular dating, phylogeography, and comparative genomic analyses of a large collection of isolates, we find that several pathogenic lineages of S. suis emerged in the 19th and 20th centuries, during an early period of growth in pig farming. These lineages have since spread between countries and continents, mirroring trade in live pigs. They are distinguished by the presence of three genomic islands with putative roles in metabolism and cell adhesion, and an ongoing reduction in genome size, which may reflect their recent shift to a more pathogenic ecology. Reconstructions of the evolutionary histories of these islands reveal constraints on pathogen emergence that could inform control strategies, with pathogenic lineages consistently emerging from one subpopulation of S. suis and acquiring genes through horizontal transfer from other pathogenic lineages. These results shed light on the capacity of the microbiota to rapidly evolve to exploit changes in their host population and suggest that the impact of changes in farming on the pathogenicity and zoonotic potential of S. suis is yet to be fully realized.

Mutation rate dynamics reflect ecological change in an emerging zoonotic pathogen.

Mutation rates vary both within and between bacterial species, and understanding what drives this variation is essential for understanding the evolutionary dynamics of bacterial populations. In this study, we investigate two factors that are predicted to influence the mutation rate: ecology and genome size. We conducted mutation accumulation experiments on eight strains of the emerging zoonotic pathogen Streptococcus suis. Natural variation within this species allows us to compare tonsil carriage and invasive disease isolates, from both more and less pathogenic populations, with a wide range of genome sizes. We find that invasive disease isolates have repeatedly evolved mutation rates that are higher than those of closely related carriage isolates, regardless of variation in genome size. Independent of this variation in overall rate, we also observe a stronger bias towards G/C to A/T mutations in isolates from more pathogenic populations, whose genomes tend to be smaller and more AT-rich. Our results suggest that ecology is a stronger correlate of mutation rate than genome size over these timescales, and that transitions to invasive disease are consistently accompanied by rapid increases in mutation rate. These results shed light on the impact that ecology can have on the adaptive potential of bacterial pathogens.

The Temporal Uses of Moral Things: Manifesting, Anchoring and Conserving Caring Relations within the Sensorium.

In this article we argue that menthol-containing products, like chewing gums, vapour rubs and mouthwashes, are used as moral things within everyday practices. They take on moral functions because of how their material qualities contribute to sensory experiences. Specifically, we focus on scenarios in which menthol products become associated with the moral work of care and highlight the temporal dimension of what people do with moral things. We review the literature on morality as a practical, everyday accomplishment and stress the embodied nature of caring practices to outline how care is bound up with sensory experience. We draw on rich qualitative data generated through creative methods, including film, photography and sketching, as part of object-elicitation interviews, focus groups, home tours and 'pop-up stalls'. We develop three concepts regarding the function of moral things: manifesting, anchoring and conserving moral relations to describe how time, morality and the sensory are entwined.

Digital technologies as truth-bearers in health care.

In this paper, we explore the idea of digital technologies as truth-bearers in health care and argue that devices like SenseCam, which facilitate reflection and memory recall, have a potentially vital role in healthcare situations when questions of veracity are at stake (e.g., when best interest decisions are being made). We discuss the role of digital technologies as truth-bearers in the context of nursing people with dementia, as this is one area of health care in which the topic of truth-telling has been hotly debated. People with dementia have been excluded from research studies and decisions that affect their lives because they are not regarded as truth-bearers-that is, as being capable of giving truthful accounts of their experiences. Also, considerable research has focused on the ethics of lying to and deceiving people with dementia. Given their increasing prominence in healthcare settings, there has been surprisingly little discussion of what role digital technologies might play in relation to these questions of truth and deception. Drawing on theories from science and technology studies (STS), we explore their possible future role in some of the truth-making processes of health care. In particular, we discuss the potential value of constraints on use of SenseCam to support the accounts of people with dementia as part of their care.

Situating anticipation in everyday life: Using sensory methods to explore public expectations of synthetic biology.

Public involvement in technological anticipation is a common feature of contemporary sociotechnical innovation. However, most engagements abstract sociotechnical futures, rather than situating them in the everyday practices in which people are routinely engaged. Recent developments in synthetic biology have established the potential for 'drop in' replacements for ingredients in consumer products, particularly in flavour and fragrance markets. This article explains how a sensory methodology can be used to explore citizens' everyday experiences and how these can be used to ground anticipation of possible sociotechnical futures. The article uses a socio-historical approach to analyse and compare two practice domains - caring for families and hygiene and personal care - to show how biosynthetic futures can disrupt existing relations between people, objects and ideas. The implications for conceptualising publics in synthetic biology and for approaches to public engagement and participation are discussed more broadly.

Engaging the Senses, Understanding Publics: Research Methods, Science Engagement, and Synthetic Biology.

Scientists and government actors often fear a 'public rejection' of biotechnology, especially regarding genetic modification. Through a research project aimed at engaging people's senses, we support an alternative way for scientists to consider non-scientists in their research.

Left to their own devices: Post-ELSI, ethical equipment and the International Genetically Engineered Machine (iGEM) Competition.

In this article, we evaluate a novel method for post-ELSI (ethical, legal and social implications) collaboration, drawing on 'human practices' (HP) to develop a form of reflexive ethical equipment that we termed 'sociotechnical circuits'. We draw on a case study of working collaboratively in the International Genetically Engineered Machine Competition (iGEM) and relate this to the parts-based agenda of synthetic biology. We use qualitative methods to explore the experience of undergraduate students in the Competition, focussing on the 2010 University of Sheffield team. We examine how teams work collaboratively across disciplines to produce novel microorganisms. The Competition involves a HP component and we examine the way in which this has been narrowly defined within the ELSI framework. We argue that this is a much impoverished style of HP when compared with its original articulation as the development of 'ethical equipment'. Inspired by this more theoretically rich HP framework, we explore the relations established between team members and how these were shaped by the norms, materials and practices of the Competition. We highlight the importance of care in the context of post-ELSI collaborations and report on the implications of our case study for such efforts and for the relation of the social sciences to the life sciences more generally.