RESUMO
In temperate and boreal ecosystems, seasonal cycles of growth and dormancy allow perennial plants to adapt to winter conditions. We show, in hybrid aspen trees, that photoperiodic regulation of dormancy is mechanistically distinct from autumnal growth cessation. Dormancy sets in when symplastic intercellular communication through plasmodesmata is blocked by a process dependent on the phytohormone abscisic acid. The communication blockage prevents growth-promoting signals from accessing the meristem. Thus, precocious growth is disallowed during dormancy. The dormant period, which supports robust survival of the aspen tree in winter, is due to loss of access to growth-promoting signals.
Assuntos
Ácido Abscísico/fisiologia , Comunicação Celular/fisiologia , Fotoperíodo , Dormência de Plantas/fisiologia , Reguladores de Crescimento de Plantas/fisiologia , Populus/crescimento & desenvolvimento , Árvores/crescimento & desenvolvimento , Ritmo Circadiano , Meristema/citologia , Meristema/crescimento & desenvolvimento , Populus/citologia , Populus/genética , Estações do Ano , Árvores/citologia , Árvores/genéticaRESUMO
The regulation of the light-harvesting antennae, the phycobilisome (Pbs), and the cpcB1A1-cpcH-cpcI-cpcD operon encoding the structural proteins of the Pbs rod, was studied in the cyanobacterium, Synechococcus sp. PCC 6301, when grown at different light intensities (li). Pbs were purified and their linker protein (LP) profiles analyzed on SDS-polyacrylamide gels. At increasing li, the amount of the distal 30-kDa LP decreased prior to any change in the amount of the proximal 33-kDa LP, indicating a sequential increase in the Pbs rod length. While the amount of LP in the rod decreased with increasing li, the levels of the LP mRNAs increased. Post-transcriptional regulation of the expression of the polycistronic cpcB1A1-cpcH-cpcI-cpcD mRNA was inferred from these observations. The half-life of the mRNAs studied was typically found to be 7 min with four exceptions: (1 and 2) the half-lives for the 3.4- and 3.7-kb polycistronic LP mRNAs were 16 and 1 min at the low (lli) and high li (hli), respectively; (3) the half-life of the 1.4-kb cpcB1A1 mRNA was 2 min at lli; and (4) the 1.3-kb cpcB1A1 transcript had a half-life of 10 min at lli. At hli, it was found that the 1.3-kb cpcB1A1 transcript did not start to disappear until the amount of the 1.4-kb cpcB1A1 transcript had reached the level equal to that of the 1.3-kb mRNA, implying that the 1.4-kb transcript might be processed to the 1.3-kb form.
Assuntos
Proteínas de Bactérias/genética , Cianobactérias/genética , Regulação Bacteriana da Expressão Gênica , Proteínas de Plantas/genética , RNA Mensageiro/metabolismo , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Sequência de Bases , Cianobactérias/metabolismo , DNA Bacteriano , Regulação Bacteriana da Expressão Gênica/efeitos da radiação , Cinética , Luz , Complexos de Proteínas Captadores de Luz , Dados de Sequência Molecular , Peptídeos/metabolismo , Ficobilissomas , Proteínas de Plantas/metabolismo , RNA Bacteriano/genéticaRESUMO
We report the draft genome of the black cottonwood tree, Populus trichocarpa. Integration of shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction of the genome. More than 45,000 putative protein-coding genes were identified. Analysis of the assembled genome revealed a whole-genome duplication event; about 8000 pairs of duplicated genes from that event survived in the Populus genome. A second, older duplication event is indistinguishably coincident with the divergence of the Populus and Arabidopsis lineages. Nucleotide substitution, tandem gene duplication, and gross chromosomal rearrangement appear to proceed substantially more slowly in Populus than in Arabidopsis. Populus has more protein-coding genes than Arabidopsis, ranging on average from 1.4 to 1.6 putative Populus homologs for each Arabidopsis gene. However, the relative frequency of protein domains in the two genomes is similar. Overrepresented exceptions in Populus include genes associated with lignocellulosic wall biosynthesis, meristem development, disease resistance, and metabolite transport.
Assuntos
Duplicação Gênica , Genoma de Planta , Populus/genética , Análise de Sequência de DNA , Arabidopsis/genética , Mapeamento Cromossômico , Biologia Computacional , Evolução Molecular , Etiquetas de Sequências Expressas , Expressão Gênica , Genes de Plantas , Análise de Sequência com Séries de Oligonucleotídeos , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/genética , Polimorfismo de Nucleotídeo Único , Populus/crescimento & desenvolvimento , Populus/metabolismo , Estrutura Terciária de Proteína , RNA de Plantas/análise , RNA não Traduzido/análiseRESUMO
Two open reading frames denoted as cpcE and cpcF were cloned and sequenced from Synechococcus sp. PCC 6301. The cpcE and cpcF genes are located downstream of the cpcB2A2 gene cluster in the phycobilisome rod operon and can be transcribed independently of the upstream cpcB2A2 gene cluster. The cpcE and cpcF genes were separately inactivated by insertion of a kanamycin resistance cassette in Synechococcus sp. PCC 7942 to generate mutants R2EKM and R2FKM, respectively, both of which display a substantial reduction in spectroscopically detectable phycocyanin. The levels of beta- and alpha-phycocyanin polypeptides were reduced in the R2EKM and R2FKM mutants although the phycocyanin and linker genes are transcribed at normal levels in the mutants as in the wild type indicating the requirement of the functional cpcE and cpcF genes for normal accumulation of phycocyanin. Two biliprotein fractions were isolated on sucrose density gradient from the R2EKM/R2FKM mutants. The faster sedimenting fraction consisted of intact phycobilisomes. The slower sedimenting biliprotein fraction was found to lack phycocyanin polypeptides, thus no free phycocyanin was detected in the mutants. Characterization of the phycocyanin from the mutants revealed that it was chromophorylated, had a lambda max similar to that from the wild type and could be assembled into the phycobilisome rods. Thus, although phycocyanin levels are reduced in the R2EKM and R2FKM mutants, the remaining phycocyanin seems to be chromophorylated and similar to that in the wild type with respect to phycobilisome rod assembly and energy transfer to the core.
Assuntos
Proteínas de Bactérias/genética , Cianobactérias/genética , Genes Bacterianos/genética , Sequência de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/fisiologia , Sequência de Bases , Mapeamento Cromossômico , Clonagem Molecular , Regulação Bacteriana da Expressão Gênica , Complexos de Proteínas Captadores de Luz , Dados de Sequência Molecular , Mutação/fisiologia , Fases de Leitura Aberta/genética , Óperon/genética , Ficobilissomas , Ficocianina/biossíntese , Ficocianina/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/isolamento & purificação , RNA Bacteriano/análise , RNA Mensageiro/análise , Análise de Sequência de DNA , Espectrometria de Fluorescência , EspectrofotometriaRESUMO
The distribution and biosynthesis of indole-3-acetic acid (IAA) was investigated during early plant development in Arabidopsis. The youngest leaves analysed, less than 0.5 mm in length, contained 250 pg mg(-1) of IAA and also exhibited the highest relative capacity to synthesize this hormone. A decrease of nearly one hundred-fold in IAA content occurred as the young leaves expanded to their full size, and this was accompanied by a clear shift in both pool size and IAA synthesis capacity. The correlation between high IAA content and intense cell division was further verified in tobacco leaves, where a detailed analysis revealed that dividing mesophyll tissue contained ten-fold higher IAA levels than tissue growing solely by elongation. We demonstrated that all parts of the young Arabidopsis plant can potentially contribute to the auxin needed for growth and development, as not only young leaves, but also all other parts of the plant such as cotyledons, expanding leaves and root tissues have the capacity to synthesize IAA de novo. We also observed that naphthylphthalamic acid (NPA) treatment induced tissue-dependent feedback inhibition of IAA biosynthesis in expanding leaves and cotyledons, but intriguingly not in young leaves or in the root system. This observation supports the hypothesis that there is a sophisticated tissue-specific regulatory mechanism for auxin biosynthesis. Finally, a strict requirement for maintaining the pool sizes of IAA was revealed as reductions in leaf expansion followed both decreases and increases in the IAA levels in developing leaves. This indicates that leaves are not only important sources for IAA synthesis, but that normal leaf expansion depends on rigorous control of IAA homeostasis.
Assuntos
Arabidopsis/crescimento & desenvolvimento , Ácidos Indolacéticos/metabolismo , Reguladores de Crescimento de Plantas/metabolismo , Divisão Celular , Retroalimentação Fisiológica , Homeostase , Mutação , Ftalimidas , Folhas de Planta/citologia , Brotos de Planta , Nicotiana/crescimento & desenvolvimentoRESUMO
We have constructed a mutant Synechococcus sp. PCC 7942, termed R2HECAT, in which the entire phycobilisome rod operon has been deleted. In the whole cell absorption spectra of R2HECAT, the peak corresponding to phycocyanin (PC), λmax≈620 nm, could not be detected. However, a single pigment-protein fraction with λmax=654 nm could be isolated on sucrose gradients from R2HECAT. Analysis of this pigment-protein fraction by non-denaturing PAGE indicates an apparent molecular mass of about 1200-1300 kDa. On exposure to low temperature, the isolated pigment-protein complex dissociated to a protein complex with a molecular mass of about 560 kDa. When analysed by SDS-PAGE, the pigment-protein fraction was found to consist of the core polypeptides but lacked PC, 27, 33, 30, and the 9 kDa polypeptides which are a part of the rods. All the chromophore bearing polypeptides of the core were found to be chromophorylated. CD as well as absorption spectra showed the expected maxima around 652 and 675 nm from allophycocyanin (APC) and allophycocyanin B (APC-B) chromophores. Low temperature fluorescence and excitation spectra also showed that the core particles were fully functional with respect to the energy transfer between the APC chromophores. We conclude that PC and therefore the rods are dispensable for the survival of Synechococcus sp. PCC 7942. The results indicate that stable and functional core can assemble in absence of the rods. These rod-less phycobilisome core is able to transfer energy to Photosystem II.
RESUMO
The metabolism of indole-3-acetic acid (IAA) was investigated in 14-d-old Arabidopsis plants grown in liquid culture. After ruling out metabolites formed as an effect of nonsterile conditions, high-level feeding, and spontaneous interconversions, a simple metabolic pattern emerged. Oxindole-3-acetic acid (OxIAA), OxIAA conjugated to a hexose moiety via the carboxyl group, and the conjugates indole-3-acetyl aspartic acid (IAAsp) and indole-3-acetyl glutamate (IAGlu) were identified by mass spectrometry as primary products of IAA fed to the plants. Refeeding experiments demonstrated that none of these conjugates could be hydrolyzed back to IAA to any measurable extent at this developmental stage. IAAsp was further oxidized, especially when high levels of IAA were fed into the system, yielding OxIAAsp and OH-IAAsp. This contrasted with the metabolic fate of IAGlu, since that conjugate was not further metabolized. At IAA concentrations below 0.5 microM, most of the supplied IAA was metabolized via the OxIAA pathway, whereas only a minor portion was conjugated. However, increasing the IAA concentrations to 5 microM drastically altered the metabolic pattern, with marked induction of conjugation to IAAsp and IAGlu. This investigation used concentrations for feeding experiments that were near endogenous levels, showing that the metabolic pathways controlling the IAA pool size in Arabidopsis are limited and, therefore, make good targets for mutant screens provided that precautions are taken to avoid inducing artificial metabolism.
Assuntos
Arabidopsis/metabolismo , Ácidos Indolacéticos/metabolismo , Reguladores de Crescimento de Plantas/metabolismo , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Cromatografia Líquida de Alta Pressão , Ácidos Indolacéticos/química , Cinética , Mutação , Reguladores de Crescimento de Plantas/química , Espectrometria de Massas de Bombardeamento Rápido de ÁtomosRESUMO
The phycobilisome rod linker genes in the two closely related cyanobacteria Synechococcus sp. PCC 6301 and Synechococcus sp. PCC 7942 were studied. Southern blot analysis showed that the genetic organization of the phycobilisome rod operon is very similar in the two strains. The phycocyanin gene pair is duplicated and separated by a region of about 2.5 kb. The intervening region between the duplicated phycocyanin gene pair was cloned from Synechococcus sp. PCC 6301 and sequenced. Analysis of this DNA sequence revealed the presence of three open reading frames corresponding to 273, 289 and 81 amino acids, respectively. Insertion of a kanamycin resistance cassette into these open reading frames indicated that they corresponded to the genes encoding the 30, 33 and 9 kDa rod linkers, respectively, as judged by the loss of specific linkers from the phycobilisomes of the insertional mutants. Amino acid compositions of the 30 and 33 kDa linkers derived from the DNA sequence were found to deviate from those of purified 33 and 30 kDa linkers in the amounts of glutamic acid/glutamine residues. On the basis of similarity of the amino acid sequence of the rod linkers between Synechococcus sp. PCC 6301 and Calothrix sp. PCC 7601 we name the genes encoding the 30, 33 and 9 kDa linkers cpcH, cpcI and cpcD, respectively. The three linker genes were found to be co-transcribed on an mRNA of 3700 nucleotides. However, we also detected a smaller species of mRNA, of 3400 nucleotides, which would encode only the cpcH and cpcI genes.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Proteínas de Bactérias/genética , Cianobactérias/genética , Genes Bacterianos/genética , Proteínas de Plantas/genética , Sequência de Aminoácidos , Proteínas de Bactérias/química , Sequência de Bases , Southern Blotting , Mapeamento Cromossômico , Complexos de Proteínas Captadores de Luz , Dados de Sequência Molecular , Família Multigênica/genética , Mutagênese Insercional , Fases de Leitura Aberta , Óperon , Ficobilissomas , Ficocianina , Proteínas de Plantas/química , RNA Mensageiro/genética , Análise de Sequência de DNA , Transcrição GênicaRESUMO
The activation of cyclin-dependent kinases (CDKs) requires phosphorylation of a threonine residue within the T-loop catalyzed by CDK-activating kinases (CAKs). Thus far no functional CAK homologue has been reported in plants. We screened an Arabidopsis cDNA expression library for complementation of a budding yeast CAK mutant. A cDNA, cak1At, was isolated that suppressed the CAK mutation in budding yeast, and it also complemented a fission yeast CAK mutant. cak1At encodes a protein related to animal CAKs. The CAK similarity was restricted to the conserved kinase domains, leading to classification of Cak1At as a distinct CDK in the phylogenetic tree. Immunoprecipitates with the anti-Cak1At antibody phosphorylated human CDK2 at the threonine residue (T160) within the T-loop and activated its activity to phosphorylate histone H1. Whereas CAKs in animals and fission yeast are involved in regulation of the cell cycle and basal transcription by phosphorylating the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II, Cak1At did not phosphorylate the CTD. An Arabidopsis CTD-kinase isolated separately from Cak1At was shown to interact with the yeast protein p13(suc1), but it had no CDK2-kinase activity. Therefore, the CTD of RNA polymerase II is probably phosphorylated by a Cdc2-related kinase distinct from Cak1At. cak1At is a single-copy gene in Arabidopsis and is highly expressed in proliferating cells of suspension cultures.
Assuntos
Proteínas de Arabidopsis , Arabidopsis/enzimologia , Quinases relacionadas a CDC2 e CDC28 , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Sequência de Aminoácidos , Divisão Celular , Clonagem Molecular , Quinase 2 Dependente de Ciclina , Quinases Ciclina-Dependentes/metabolismo , DNA Complementar/genética , Ativação Enzimática , Expressão Gênica , Genes de Plantas , Teste de Complementação Genética , Humanos , Dados de Sequência Molecular , Fosforilação , Filogenia , Proteínas Serina-Treonina Quinases/química , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Quinase Ativadora de Quinase Dependente de CiclinaRESUMO
cDNAs encoding cyclin H homologs were isolated from poplar (Populus tremula X tremuloides) and rice (Oryza sativa) plants, and were designated Pt;cycH;1 and Os;cycH;1, respectively. The deduced amino-acid sequences showed 40-60% similarity to human cyclin H and Schizosaccharomyces pombe Mcs2, with higher similarity in the cyclin box region. While Pt;cycH;1 and Os;cycH;1 were expressed in all tissues examined, the transcripts accumulated abundantly in dividing cells. Expression of Os;cycH;1 was abundant in the S-phase in partially synchronized suspension cells, and was induced by submergence in internodes of deepwater rice. A yeast two-hybrid assay demonstrated that both Pt;CycH;1 and Os;CycH;1 were able to interact with rice R2 kinase, which is structurally and functionally similar to cyclin-dependent kinase (CDK)-activating kinase (CAK) of vertebrates. Moreover, an in vitro pull-down assay showed that Os;CycH;1 specifically bound to R2 but not to other rice CDKs. When R2 was expressed in budding yeast CAK mutant, the suppression activity in terms of temperature-sensitivity was enhanced by co-expression with Os;cycH;1. Furthermore, in vitro kinase assay indicated that the kinase activities of R2 on CDKs and the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II were markedly elevated by binding to Os;CycH;1. Our results suggest that cyclin H is a regulatory subunit of CAK, which positively controls CDK- and CTD-kinase activities in plant cells.
Assuntos
Quinases relacionadas a CDC2 e CDC28 , Ciclo Celular , Ciclinas/genética , Ciclinas/metabolismo , Oryza/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Árvores/metabolismo , Sequência de Aminoácidos , Animais , Clonagem Molecular , Quinase 2 Dependente de Ciclina , Quinases Ciclina-Dependentes/genética , Quinases Ciclina-Dependentes/metabolismo , Ciclinas/química , Ativação Enzimática , Humanos , Dados de Sequência Molecular , Oryza/citologia , Oryza/genética , Filogenia , Proteínas Serina-Treonina Quinases/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Árvores/citologia , Árvores/genética , Quinase Ativadora de Quinase Dependente de CiclinaRESUMO
Recent research has highlighted the importance of auxin concentration gradients during plant development. Establishment of these gradients is believed to involve polar auxin transport through specialized carrier proteins. We have used an experimental system, the wood-forming tissue of hybrid aspen, which allows tissue-specific expression analysis of auxin carrier genes and quantification of endogenous concentrations of the hormone. As part of this study, we isolated the putative polar auxin transport genes, PttLAX1-PttLAX3 and PttPIN1-PttPIN3, belonging to the AUX1-like family of influx and PIN1-like efflux carriers, respectively. Analysis of PttLAX and PttPIN expression suggests that specific positions in a concentration gradient of the hormone are associated with different stages of vascular cambium development and expression of specific members of the auxin transport gene families. We were also able demonstrate positive feedback of auxin on polar auxin transport genes. Entry into dormancy at the end of a growing season leads to a loss of auxin transport capacity, paralleled by reduced expression of PttLAX and PttPIN genes. Furthermore, data from field experiments show that production of the molecular components of the auxin transport machinery is governed by environmental controls. Our findings collectively demonstrate that trees have developed mechanisms to modulate auxin transport in the vascular meristem in response to developmental and environmental cues.
Assuntos
Ácidos Indolacéticos/metabolismo , Árvores/metabolismo , Sequência de Bases , Transporte Biológico Ativo/genética , Clonagem Molecular , DNA de Plantas/genética , Meio Ambiente , Expressão Gênica , Genes de Plantas , Dados de Sequência Molecular , Estações do Ano , Árvores/genética , Árvores/crescimento & desenvolvimentoRESUMO
Lateral root development in Arabidopsis provides a model for the study of hormonal signals that regulate postembryonic organogenesis in higher plants. Lateral roots originate from pairs of pericycle cells, in several cell files positioned opposite the xylem pole, that initiate a series of asymmetric, transverse divisions. The auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) arrests lateral root development by blocking the first transverse division(s). We investigated the basis of NPA action by using a cell-specific reporter to demonstrate that xylem pole pericycle cells retain their identity in the presence of the auxin transport inhibitor. However, NPA causes indoleacetic acid (IAA) to accumulate in the root apex while reducing levels in basal tissues critical for lateral root initiation. This pattern of IAA redistribution is consistent with NPA blocking basipetal IAA movement from the root tip. Characterization of lateral root development in the shoot meristemless1 mutant demonstrates that root basipetal and leaf acropetal auxin transport activities are required during the initiation and emergence phases, respectively, of lateral root development.
Assuntos
Arabidopsis/crescimento & desenvolvimento , Ácidos Indolacéticos/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Arabidopsis/metabolismo , Arabidopsis/fisiologia , Transporte Biológico , Diferenciação Celular , Divisão Celular , Polaridade Celular , Ácidos Indolacéticos/antagonistas & inibidores , Ácidos Indolacéticos/fisiologia , Meristema/anatomia & histologia , Meristema/crescimento & desenvolvimento , Meristema/metabolismo , Ftalimidas/farmacologia , Raízes de Plantas/anatomia & histologia , Raízes de Plantas/metabolismo , Transdução de SinaisRESUMO
Mutation of the PRL1 gene, encoding a regulatory WD protein, results in glucose hypersensitivity and derepression of glucose-regulated genes in Arabidopsis. The yeast SNF1 protein kinase, a key regulator of glucose signaling, and Arabidopsis SNF1 homologs AKIN10 and AKIN11, which can complement the Deltasnf1 mutation, were found to interact with an N-terminal domain of the PRL1 protein in the two-hybrid system and in vitro. AKIN10 and AKIN11 suppress the yeast Deltasnf4 mutation and interact with the SNF4p-activating subunit of SNF1. PRL1 and SNF4 bind independently to adjacent C-terminal domains of AKIN10 and AKIN11, and these protein interactions are negatively regulated by glucose in yeast. AKIN10 and AKIN11, purified in fusion with glutathione S-transferase, undergo autophosphorylation and phosphorylate a peptide of sucrose phosphate synthase in vitro. The sucrose phosphate synthase-peptide kinase activity of AKIN complexes detected by immunoprecipitation is stimulated by sucrose in light-grown Arabidopsis plants. In comparison with wild type, the activation level of AKIN immunocomplexes is higher in the prl1 mutant, suggesting that PRL1 is a negative regulator of Arabidopsis SNF1 homologs. This conclusion is supported by the observation that PRL1 is an inhibitor of AKIN10 and AKIN11 in vitro.